CASE™ Cellular Activation of Signaling ELISA

The Cellular Activation of Signaling ELISA or CASE Kits monitor the activation of signal transduction pathways by analyzing the phosphorylation of their upstream regulators. Each CASE™ Kit is a cell-based ELISA and includes a complete antibody-based detection system for the colorimetric quantification of the phosphorylated form and the total amount of a specific protein. A greater extent of phosphorylation indicates an increased stimulation of the corresponding downstream pathway. Use these kits to directly determine the extent of protein phosphorylation and pathway activation after treatment with a specific drug or inhibitor without the need to prepare cell lysates.

Quantitative: Cell-based ELISA determines level of total and activated form of protein at same time.

Simple: Easy, quantitative, and non-radioactive protocol with minimal hands-on time

No Extractions: Directly measure protein phosphorylation state on cells fixed in a 96-well culture plate

Learn more about CASE Kits, download our technology brochure [here].
For a complete overview of the CASE kit technology, click [here].

Features of the CASE™ Kits:

Western blot analysis and radioactive in vitro kinase assays have found widespread use in the monitoring of kinase activation. However, both methods suffer from distinct disadvantages. These tedious multi-step methods require a large amount of cells and treatment reagents, and are not suitable for high-throughput studies. They also involve making cell lysates, a process during which protein phosphorylation status can be lost.

CASE Kits overcome these difficulties, requiring no protein extraction and very little hands-on-time. Now you can assay phosphorylation of important proteins in a convenient 96-well plate format that is easily scaled up for screening experiments. The CASE kits may also be seen as high-throughput method for analyzing the phosphorylation status of specific proteins inside cells.

SABiosciences provides a full range of solutions for your needs to study the function of genes:

• Find a CASE for your favorite protein or pathway by browsing through our Product Listing.
  
• Find the right kinase inhibitor to control for specificity in your assay [here].
  
• Read [this] about the reagents & equipment required to use the CASE Kit in your lab.
  
• For CASE Kit Pricing and other Ordering Instructions, click [here].