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Home > Products > ELISArray Kits > Multi-Analytes > Human Inflammatory Cytokines Multi-Analyte ELISArray Kit: MEH-004A
Human Inflammatory Cytokines Multi-Analyte ELISArray Kit: MEH-004A |
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| Description |
The Human Inflammatory Cytokines &
Chemokines Multi-Analyte ELISArray Kit analyzes a panel of 12
pro-inflammatory cytokines using a conventional ELISA protocol all at once
under uniform conditions. The cytokines & chemokines represented by
this array are IL1A, IL1B, IL2, IL4, IL6, IL8, IL10, IL12, IL17A, IFNg,
TNFa, and GM-CSF. The pro-inflammatory cytokines IL1, IL6, and TNFa are
also endogenous pyrogens. IL4 and IL10 can also act as anti-inflammatory
cytokines depending on their concentrations. The complete kit features the
best possible combination of capture and detection antibodies for each
protein in the panel and all of the needed colorimetric detection
reagents. Using the same ELISA protocol and development or incubation
time, you can easily profile the levels of a focused panel of 12 proteins
related to inflammation with this array.
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| Product Resources |
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| Protein Panel: |
| IL1A, IL1B, IL2, IL4, IL6, IL8, IL10, IL12, IL17A, IFNγ, TNFα,
GM-CSF |
| What is a Multi-Analyte ELISArray Kit? |
| The ELISArray Cytokine and Chemokine Kits are the simplest
simultaneous multi-analyte enzyme-linked immunosorbent assays (ELISA) in the
market. The ELISArray Kits are designed to survey a specific panel of
cytokines or chemokines involved in autoimmunity, inflammation, or T-cell
biology in cell culture supernatant, serum or plasma. The ELISArray Kits can
be used for autoimmune and immune disorder, cancer, immunology, infectious
disease, or any other area of biological and medical research normally using
individual ELISA kits.
Need more information about ELISArray Kits?
Please visit ELISArray Kit home page or send
an Email to Technical Support. |
| Kit Contents / Storage Conditions/
Shelf Life |
| Please check the kit components immediately after you receive this package. SABiosciences is only responsible for missing items reported within two (2) business days of receipt.
Enough reagents are provided to process the included plate of 12 ELISA
strips.
| Component / Description |
Quantity |
| BOX 1: Shipped on blue ice packs. Store
at -20 ºC. |
| Avidin-HRP Conjugate |
One 1.5-ml tubes |
| 10% BSA |
15 ml bottle |
| Donkey Serum |
15 ml bottle |
| BOX 2: Shipped at ambient temperature.
Store at 4 ºC. |
| 96-well pre-coated Capture Antibody microplate |
One plate of 12 strips in a pouch |
| Detection Antibody Dilution Tube Strip |
One strip of 12 tubes |
| Sample Dilution Buffer Stock |
60 ml bottle |
| Assay Buffer Stock |
60 ml bottle |
| Wash Buffer (10 × Concentrate) |
125 ml bottle |
| Development Solution |
25 ml bottle |
| Stop Solution |
60 ml bottle |
| BOX 3: Shipped on blue ice packs.
Store at -20 ºC. |
| Antigen Standards |
One box of 12 1.5-ml tubes |
| Detection Antibodies |
One box of 12 1.5-ml tubes |
Shelf Life: Do not use kit beyond the expiration date printed on the label. |
| Brief Protocol |
The Brief Protocol is meant for experience
users only. First-time users should refer to the User
Manual.
- Prepare replicate serial dilutions of the Antigen Standard and your
experimental samples.
- Pipette 50 µl of Assay Buffer into each well of the 8-well ELISA
strips.
- Transfer 50 µl samples and/or standards to the appropriate wells of
the ELISA strips.
- Gently shake or tap plate for 10 seconds. Incubate for 2 hours at room
temperature.
- Washing ELISA Wells:
Decant or aspirate well contents. Add 350 µl 1 × Washing Buffer.
Gently shake or tap plate for 10 seconds. Decant or aspirate. Blot array
upside down on absorbent paper to remove any residual buffer. Repeat
wash twice more.
- Pipette 100 µl of Detection Antibody solution. Incubate 1 hour at room
temperature.
- Wash ELISA wells as described above.
- Add 100 µl Avidin-HRP solution to all wells. Incubate for 30 minutes at
room temperature.
- Wash ELISA wells for a total of 4 washes.
- Add 100 µl of Development Solution to each well. Incubate the plate
for 15 minutes at room temperature in the dark.
- Add 100 µl of Stop Solution to each well. The color changes from
blue to yellow.
- Read absorbance at 450 nm within 30 minutes of stopping the reaction. If
wavelength correction is available, subtract readings at 570 nm from
the reading at 450 nm.
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