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Mouse Common Cytokines Multi-Analyte ELISArray Kit: MEM-006A

Description
The Mouse Common Cytokines Multi-Analyte ELISArray Kit analyzes a panel of 12 important cytokines using a conventional ELISA protocol all at once under uniform conditions. The cytokines represented by this array are IL1A, IL1B, IL2, IL4, IL5, IL6, IL10, IL12, IL13, IL17A, G-CSF, and GM-CSF. IL1A, IL1B, IL2, IL6, IL12, IL17A, and G-CSF, GM-CSF are all pro-inflammatory cytokines. IL4 and IL10 can act as either pro- or anti-inflammatory cytokines depending on their concentration, while IL13 is exclusivley and anti-inflammatory cytokine. IL1 and IL6 are also reported to be endogenous pyrogens. The complete kit features the best possible combination of capture and detection antibodies for each protein in the panel and all of the needed colorimetric detection reagents. Using the same ELISA protocol and development or incubation time, you can easily profile the levels of a focused panel of 12 common cytokines with this array.
Product Resources
Pricing and Ordering Related Array Products Modify this ELISArray Kit
Protein Panel:
IL1A, IL1B, IL2, IL4, IL5, IL6, IL10, IL12, IL13, IL17A, G-CSF, GM-CSF
What is a Multi-Analyte ELISArray Kit?
The ELISArray Cytokine and Chemokine Kits are the simplest simultaneous multi-analyte enzyme-linked immunosorbent assays (ELISA) in the market. The ELISArray Kits are designed to survey a specific panel of cytokines or chemokines involved in autoimmunity, inflammation, or T-cell biology in cell culture supernatant, serum or plasma. The ELISArray Kits can be used for autoimmune and immune disorder, cancer, immunology, infectious disease, or any other area of biological and medical research normally using individual ELISA kits.

Need more information about ELISArray Kits?
Please visit ELISArray Kit home page or send an Email to Technical Support.

Kit Contents / Storage Conditions/ Shelf Life
Please check the kit components immediately after you receive this package. SABiosciences is only responsible for missing items reported within two (2) business days of receipt.

Enough reagents are provided to process the included plate of 12 ELISA strips.

Component / Description  Quantity
BOX 1: Shipped on blue ice packs. Store at -20 ºC.
    Avidin-HRP Conjugate  One 1.5-ml tubes
    10% BSA 15 ml bottle
Donkey Serum 15 ml bottle
BOX 2: Shipped at ambient temperature. Store at 4 ºC.
96-well pre-coated Capture Antibody microplate One plate of 12 strips in a pouch
Detection Antibody Dilution Tube Strip  One strip of 12 tubes
Sample Dilution Buffer Stock  60 ml bottle
Assay Buffer Stock  60 ml bottle
Wash Buffer (10 × Concentrate)  125 ml bottle
Development Solution  25 ml bottle
Stop Solution 60 ml bottle
BOX 3: Shipped on blue ice packs. Store at -20 ºC.
Antigen Standards  One box of 12 1.5-ml tubes
Detection Antibodies One box of 12 1.5-ml tubes

Shelf Life: Do not use kit beyond the expiration date printed on the label.

Brief Protocol
The Brief Protocol is meant for experience users only. First-time users should refer to the User Manual.
  1. Prepare replicate serial dilutions of the Antigen Standard and your experimental samples.
  2. Pipette 50 µl of Assay Buffer into each well of the 8-well ELISA strips.
  3. Transfer 50 µl samples and/or standards to the appropriate wells of the ELISA strips.
  4. Gently shake or tap plate for 10 seconds. Incubate for 2 hours at room temperature.
  5. Washing ELISA Wells:
    Decant or aspirate well contents. Add 350 µl 1 × Washing Buffer. Gently shake or tap plate for 10 seconds. Decant or aspirate. Blot array upside down on absorbent paper to remove any residual buffer. Repeat wash twice more.
  6. Pipette 100 µl of Detection Antibody solution. Incubate 1 hour at room temperature.
  7. Wash ELISA wells as described above.
  8. Add 100 µl Avidin-HRP solution to all wells. Incubate for 30 minutes at room temperature.
  9. Wash ELISA wells for a total of 4 washes.
  10. Add 100 µl of Development Solution to each well. Incubate the plate for 15 minutes at room temperature in the dark.
  11. Add 100 µl of Stop Solution to each well. The color changes from blue to yellow.
  12. Read absorbance at 450 nm within 30 minutes of stopping the reaction. If wavelength correction is available, subtract readings at 570 nm from the reading at 450 nm.