| Description |
| The Rat IFNγ Single Analyte ELISArray
Kit is designed to quantitatively measure the amount of this cytokine in
cell culture supernatant, serum or plasma using a conventional
enzyme-linked immunosorbent assay (ELISA). Each 96-well plate (8
wells/strip, 12 strips) is coated with the protein-specific capture
antibody. We screened all commercially available antibodies to identify
the best capture and detection antibodies. The high sensitivity, good
linearity and low background of the Single Analyte ELISArray Kits provide
reliable and reproducible results for your cytokine and chemokine
analyses.
|
|
|
| Product Resources |
|
|
| More information on IFNγ from
NCBI: |
| "In contrast to interferon-α and interferon-β which can be expressed by all cells, IFN-γ is secreted by T lymphocytes and NK cells only. Also known as immune interferon, IFN-γ is the only Type II interferon. It is serologically distinct from Type I interferons and it is acid-labile, while the type I variants are acid-stable. IFN-γ has antiviral, immunoregulatory, and anti-tumour properties. It alters transcription in up to 30 genes producing a variety of physiological and cellular responses. Activation by IFN-γ is achieved by its interaction with a heterodimeric receptor consisting of IFNGR1 & IFNGR2 (interferon gamma receptors). IFN-γ binding to the receptor activates the JAK-STAT pathway. In addition, IFN-γ activates APCs and promotes Th1 differentiation by upregulating the transcription factor T-bet. IFN-γ is the hallmark cytokine of Th1 cells (Th2 cells produce IL-4). NK cells and CD8+ cytotoxic T cells also produce IFN-γ. IFN-γ suppresses osteoclast formation by rapidly degrading the RANK adaptor protein TRAF6 in the RANK-RANKL signaling pathway, which otherwise stimulates the production of NFκB." |
| Kit Contents / Storage Conditions/
Shelf Life |
| Please check the kit components immediately after you receive this package. SABiosciences is only responsible for missing items reported within two (2) business days of receipt.
Enough reagents are provided to process the included plate of 12 ELISA
strips.
| Component / Description |
Quantity |
| BOX 1: Shipped on blue ice packs. Store
at -20 ºC. |
| Antigen Standard (1 µg/ml) |
1.5-ml tube |
| Detection Antibody |
1.5-ml tube |
| Avidin-HRP Conjugate |
1.5-ml tube |
| 10% BSA |
15 ml bottle |
| BOX 2: Shipped at ambient temperature.
Store at 4 ºC. |
| Pre-coated Capture Antibody 8-well strips |
One plate of 12 strips in a pouch |
| Sample Dilution Buffer Stock |
60 ml bottle |
| Assay Buffer Stock |
60 ml bottle |
| Wash Buffer (10 × Concentrate) |
125 ml bottle |
| Development Solution |
25 ml bottle |
| Stop Solution |
60 ml bottle |
Shelf Life: Do not use kit beyond the expiration date printed on the label. |
| Brief Protocol |
The Brief Protocol is meant for experience
users only. First-time users should refer to the User
Manual.
- Prepare replicate serial dilutions of the Antigen Standard and your
experimental samples.
- Pipette 50 µl of Assay Buffer into each well of the 8-well ELISA
strips.
- Transfer 50 µl samples and/or standards to the appropriate wells of
the ELISA strips.
- Gently shake or tap plate for 10 seconds. Incubate for 2 hours at room
temperature.
- Washing ELISA Wells:
Decant or aspirate well contents. Add 350 µl 1 × Washing Buffer.
Gently shake or tap plate for 10 seconds. Decant or aspirate. Blot array
upside down on absorbent paper to remove any residual buffer. Repeat
wash twice more.
- Pipette 100 µl of Detection Antibody solution. Incubate 1 hour at room
temperature.
- Wash ELISA wells as described above.
- Add 100 µl Avidin-HRP solution to all wells. Incubate for 30 minutes at
room temperature.
- Wash ELISA wells for a total of 4 washes.
- Add 100 µl of Development Solution to each well. Incubate the plate
for 15 minutes at room temperature in the dark.
- Add 100 µl of Stop Solution to each well. The color changes from
blue to yellow.
- Read absorbance at 450 nm within 30 minutes of stopping the reaction. If
wavelength correction is available, subtract readings at 570 nm from
the reading at 450 nm.
|
Complete Array List
