Protein Phosphorylation Assays

Monitoring protein phosphorylation of key molecules provides insight into the activation of signal transduction pathways. SABiosciences provides a comprehensive suite of tools for monitoring protein phosphorylation based on two techniques. The first is a cell based assay for measuring related phosphorylation. The second set of assays are ELISA-based for measuring up to eight analytes simultaneously.

CASE™ kits - Cellular Activation of Signaling ELISA
Each CASE Kit is a cell-based ELISA and includes a complete antibody-based detection system for the colorimetric quantification of the phosphorylated form and the total amount of a specific protein. Use these kits to directly determine the extent of protein phosphorylation and pathway activation after treatment with a specific drug or inhibitor without the need to prepare cell lysates. See the product listing

Quantitative: Cell-based ELISA determines level of total and activated form of protein at same time.
Simple: Easy, quantitative, and non-radioactive protocol with minimal hands-on time
No Extractions: Directly measure protein phosphorylation state on cells fixed in a 96-well culture plate

PhosphoELISArray™ Kits - Lysate-Based ELISA
PhosphoELISArray Kits provide the simplest way to monitor phosphorylation of key signaling proteins. Multi-Analyte PhosphoELISArray Kits profile relative phosphorylation of a panel of proteins in multiple samples at the same time. Single Analyte PhosphoELISArray kits measure amounts of individual phosphorylated proteins in up to 96 samples. The combination of Multi-Analyte and Single Analyte PhosphoELISArray Kits enables rapid screening for changes in several signaling pathways and then to follow up with more rigorous studies. See the product listing

Pathway Focused: Multi-Analyte PhosphoELISArrays determine relative phosphorylation of multiple signaling proteins simultaneously.
Rapid: Easy, quantitative, and non-radioactive ELISA protocol with no additional reagents required.
Scalable: Directly measure protein phosphorylation state on 96 cell lysates.

SABiosciences provides a full range of solutions for your needs to study the function of genes:
Find a CASE for your favorite protein by browsing through our Product Listing.
Find a PhosphoELISArray for your favorite protein or pathway through our Product Home page.
Find the right kinase inhibitor to control for specificity in your assay.