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Protein Phosphorylation Assays

Monitoring protein phosphorylation of key molecules provides insight into the activation of signal transduction pathways. SABiosciences provides a comprehensive suite of tools for monitoring protein phosphorylation based on two techniques. The first is a cell based assay for measuring related phosphorylation. The second set of assays are ELISA-based for measuring up to eight analytes simultaneously.

CASE™ kits - Cellular Activation of Signaling ELISA
Each CASE Kit is a cell-based ELISA and includes a complete antibody-based detection system for the colorimetric quantification of the phosphorylated form and the total amount of a specific protein. Use these kits to directly determine the extent of protein phosphorylation and pathway activation after treatment with a specific drug or inhibitor without the need to prepare cell lysates. See the product listing

Quantitative: Cell-based ELISA determines level of total and activated form of protein at same time.
Simple: Easy, quantitative, and non-radioactive protocol with minimal hands-on time
No Extractions: Directly measure protein phosphorylation state on cells fixed in a 96-well culture plate

PhosphoELISArray™ Kits - Lysate-Based ELISA
PhosphoELISArray Kits provide the simplest way to monitor phosphorylation of key signaling proteins. Multi-Analyte PhosphoELISArray Kits profile relative phosphorylation of a panel of proteins in multiple samples at the same time. Single Analyte PhosphoELISArray kits measure amounts of individual phosphorylated proteins in up to 96 samples. The combination of Multi-Analyte and Single Analyte PhosphoELISArray Kits enables rapid screening for changes in several signaling pathways and then to follow up with more rigorous studies. See the product listing

Pathway Focused: Multi-Analyte PhosphoELISArrays determine relative phosphorylation of multiple signaling proteins simultaneously.
Rapid: Easy, quantitative, and non-radioactive ELISA protocol with no additional reagents required.
Scalable: Directly measure protein phosphorylation state on 96 cell lysates.

SABiosciences provides a full range of solutions for your needs to study the function of genes:
Find a CASE for your favorite protein by browsing through our Product Listing.
Find a PhosphoELISArray for your favorite protein or pathway through our Product Home page.
Find the right kinase inhibitor to control for specificity in your assay.

Phosphorylation CASE Kits
CASE Product Listing
PhosphoELISArray