Inhibitors for Functional Assays:

The Inhibitors for Functional Assays are designed to help you determine the specificity of the pathway activation observed with a Gene Function Assay, such as a CASE kit. These chemical compounds directly inhibit the enzymatic activation of a pathway's regulator. For example, the inhibition of a kinase (such as PI3K) prevents the phosphorylation and activation of the next kinase downstream (AKT) in its cascade. Test specificity by simply performing control assays in the presence of your experimental stimulus with or without the appropriate inhibitor. If signal is only detected in the absence of the inhibitor, then the upstream regulator specifically activated the pathway.

Product Listing:

Ready-to-Use Convenience:

The inhibitors are shipped already dissolved at convenient stock concentrations ready to dilute and use. There is no need to calculate volumes or re-suspend the chemicals; you only need to calculate the appropriate dilution factors. Add the dilutions directly to your cell culture medium to pre-treat cells before adding your experimental stimulus to activate the protein and / or pathway under study. (For best results, use serum-free medium.)