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GeneRead Library Quant Array

NGS Platform

Plate Format

# Plates

# Samples

Catalog #

Illumina

96-/100-well (A/C/D/F/R)

2

26

NGIL-002Z

384-well (E/G)

1

45

NGIL-3002Z

Ion Torrent

96-/100-well (A/C/D/F/R)

2

26

NGIT-002Z

384-well (E/G)

1

45

NGIT-3002Z

One of the most important factors in a next-generation sequencing experiment is the accurate quantification of the prepared library.

The GeneRead Library Quant Array uses real-time PCR to quantify NGS libraries by specifically quantifying DNA molecules with adaptors at both ends, which are the only amplifiable molecules during emulsion PCR (Ion Torrent platform) or bridge PCR (Illumina platform) and therefore provides highly accurate quantification of amplifiable library molecules. The high sensitivity of real-time PCR allows quantification of libraries with very low concentrations, even below the detection threshold of conventional spectrophotometric methods.

The GeneRead Library Quant Array contains five pre-dispensed, sequential 10-fold dilutions of DNA Standard mixed with a PCR primer assay in triplicate, as well as PCR primer assays in the remaining wells of a 96-well, 384-well PCR plate or 100-wellring. The pre-dispensed serially diluted DNA standards and PCR primer assay provide a highly convenient method for the quantification of amplifiable library molecules.

The GeneRead Library Quant Array is optimized with GeneRead qPCR SYBR� Green Mastermixes to provide superior sensitivity and wide dynamic ranges.  

Why GeneRead Library Quant Array?

  • Ready-to-use, pre-dispensed sequentially diluted DNA standard eliminates manual titration
  • Data analysis Excel sheet automatically calculates library concentration
  • Simple protocol, high sensitivity and wide dynamic range
  • Compatible with all major NGS platforms and qPCR instruments

How it works


Figure 1.� GeneRead Library Quant Array workflow.
Performance Consistency


Figure 2. Reliable NGS library quantification with minimal variability of DNA standards from lot-to-lot.

The DNA standard for Illumina MiSeq platform from three different lots were used to prepare five sequential 10-fold dilutions. The diluted DNA standards were mixed with library specific PCR primer assay and GeneRead qPCR SYBR Green mastermix (from three different lots), and subjected to real-time PCR. The minimal variation in Ct values for diluted DNA standards from three different lots confirms the reliability of GeneRead Library Quant System.

Application Data

GeneRead Library Quantification System enables quantification of libraries with concentration below detection limit of conventional methods


NGS libraries were constructed for Ion Torrent PGM platform using Human Comprehensive Cancer GeneRead DNAseq Gene Panel from universal genomic DNA (NGS-L1) and genomic DNA isolated from FFPE lung carcinoma sample (NGS-L2) and subjected to quantification by High Sensitive DNA Assay Kit (BioAnalyzer). BioAnalyzer can quantify only NGS-L1 whereas concentration of NGS-L2 was too low to quantify (A). GeneRead Library Quant System�s high sensitivity and broad dynamic range enables the quantification of both NGS-L1 and NGS-L2. The result of library quantification for NGS-L1(blue arrow) and NGS-L2(red arrow) with 1:2000 dilution is shown in figure B. Amount of NGS-L1 and NGS-L2 amount obtained by both methods are summarized in Figure C.

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