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GeneRead Library Quantification System

The GeneRead Library Quantification System uses real time PCR to quantify NGS libraries. The GeneRead Library Quantification System specifically quantifies DNA molecules with adaptors at both ends, which are the only amplifiable molecules during emulsion PCR and therefore provides highly accurate quantification of amplifiable library molecules. The high sensitivity of real time PCR allows quantification of libraries with very low concentration, even below the detection threshold of conventional spectrophotometric methods.

The GeneRead Library Quantification System is optimized with GeneRead SYBR Green Mastermixes to provide superior sensitivity and wide linear dynamic ranges. It can be easily automated for high throughput applications.

The GeneRead Library Quantification System is compatible with the following NGS platforms:

  1. Illumina
    1. HiSeq (1000, 2000, 2500)
    2. MiSeq
    3. Genome Analyze IIx
  2. Life Technologies
    1. Ion Torrent PGM
    2. Ion Proton

The GeneRead Library Quantification System is available for the following applications:

  1. Quantification of DNA after targeted PCR-enabled amplification with the GeneRead DNAseq Gene Panel System
    1. Monitor efficiency of PCR-enrichment of targeted genes of interest with integrated controls
  2. Quantification of DNA for any applications
    1. Whole genome
    2. Exome
    3. RNA-seq / transcriptome
    4. ChIP-seq
    5. Methylation
    6. Targeted amplification of GOIs with any other system

The GeneRead Library Quantification System is available in the following formats:

  1. GeneRead DNAseq Library Quant Array
  2. PCR Array format for QC of target enrichment process and quantification of DNA library in GeneRead DNAseq Gene Panel application; compatible with either block (96-/384-well plate) or disc (100-well-disc) RT-PCR instruments; no titration of DNA standard needed. �

  3. GeneRead Library Quant Array
  4. PCR Array format for quantification of DNA library in all other NGS applications; compatible with either block (96-/384-well plate) or disc (100-well-disc) RT-PCR instruments; no titration of DNA standard needed.

  5. GeneRead Library Quant Kit
  6. Tube assay format for quantification of DNA library in all other NGS applications, compatible with most RT-PCR instruments; titration of DNA standard required.

NGS System Home
DNAseq Targeted Panels
DNAseq Mix-n-Match Panels
DNAseq Custom Panels
DNAseq Panel Mastermix
GeneRead Library Quantification
Library Quantification Mastermix