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GeneRead Library Quantification Mastermix

GeneRead qPCR SYBR Green Mastermix

Included Reference Dye*


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2 96-well plates /
1 384-well plate






FAST with ROX (for RGQ)

2 100-well RGQ discs


*Please check your instrument to determine which reference dye is required for your instrument.

The GeneRead qPCR SYBR® Green Mastermixes contain all of the optimized reagents and buffers needed for SYBR® Green based real-time polymerase chain reactions in all real-time PCR instrumentation. Each Mastermix includes real-time PCR buffer, a high-performance HotStart DNA Taq polymerase, nucleotides, and a reference dye (ROX or Fluorescein) if and when needed to normalize a specific instruments' optics. Simply add the Mastermix to PCR tubes along with your template and primers. The chemically-modified and tightly controlled HotStart enzyme uniquely provides more accurate SYBR® Green results by preventing the amplification of primer dimers and other non-specific products.

Two (2) tubes each containing 1.35 mL of 2X solution and enough for 100 standard 25-µl reactions

Please check the kit components immediately after you receive this package. We are not responsible for any missing items not reported within two (2) business days upon receipt.

Storage Conditions:
The kit is shipped on dry ice or cold packs. For long-term storage, keep at -20 ºC. If entire volume is not be used all at once, divide into aliquots and store at -20 ºC. Avoid repeated freezing and thawing.

Shelf Life:
All reagents are stable for 6 months after receipt if stored at the recommended temperature.

SYBR® is a registered trademark of Molecular Probes, Inc.; SmartCycler® is a registered trademark of Cepheid; LightCycler® is a registered trademark of Roche Diagnostics; ABI® is a registered trademark of Applera Corporation; FAM™ is a trademark of Applera Corporation; ROX™ is a trademark of Applera Corporation; Bio-Rad® and iCycler® are registered trademarks of Bio-Rad Laboratories; Chromo4™ iQ™, MyiQ™ and Opticon™ are trademarks of Bio-Rad Laboratories; Stratagene® is a registered trademark of Stratagene Corporation; Eppendorf® and Mastercycler® are registered trademarks of Eppendorf AG.

Use of this product is covered by one of more of the following US patents and corresponding patent claims outside the US: 5,079,352, 5,789,224, 5,618,711, 6,127,155, 5,677,152 (claims 1 to 23 only), 5,773,258 (claims 1 and 6 only), 5,407,800, 5,322,770, 5,310,652, 5,994,056, 6,171,785, and claims outside the US corresponding to US Patent No. 4,889,818. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

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