RT² Profiler™ PCR Array
PCR Arrays are the most reliable tools for analyzing the expression of a
focused panel of genes. Each 96-well plate, 384-well plate, or 100-well disc PCR Array includes SYBR Green-optimized primer
assays for a thoroughly researched panel of relevant, pathway- or
disease-focused genes. PCR Arrays can also be customized to contain a panel of
genes tailored to your specific research interests. Our high-quality primer
design and master mix formulation enable the PCR Array to amplify 96 or 384 different
gene-specific products simultaneously under uniform cycling conditions. This
combination provides the PCR Array with the specificity and the high
amplification efficiencies required for accurate real-time SYBR Green results.
The simplicity of the PCR Arrays makes them accessible for routine use in every
New developments in PCR Array technology enable optimal performance of RT² Profiler PCR Arrays using RNA prepared from regular samples (0.1 - 5 ug RNA), FFPE samples, and small samples (1 - 100 ng RNA). See the PCR Array Protocol Guide to learn more.
Why PCR Arrays?
the sensitivity of the RT² First Strand Kit, as little as 1 ng or as much as
5 µg of total RNA per array plate provides greater than 80 percent
present call rates.
The high reproducibility of the system, with replicate correlation coefficients
> 0.99, means that experimental samples can be reliably compared across
plates and runs.
specificity of the system, imparted by the combination of SYBR Green primers
and PCR master mixes, guarantees a single product of the predicted size from
every reaction without secondary products such as primer dimers.
The complete PCR Array System guarantees
the optimal performance of the PCR Array. Including the optional RT² RNA QC
PCR Array into the complete system maximizes control over consistent RNA
The PCR Arrays have been used for cancer, immunology, stem cells, toxicology, and many other areas of biological and medical research. To view application examples for cancer and toxicology research, click [ here ]. To see a list of publications citing the use of the PCR Array, click [ here
How It Works
Simply mix your cDNA
template with the appropriate ready-to-use PCR master mix, aliquot equal
volumes to each well of the same plate, and then run the real-time PCR cycling
program. PCR Arrays are compatible with all ABI, Bio-Rad, Eppendorf, QIAGEN, Roche, and Stratagene instrumentation. Find the correct setup instructions and protocol files [ here
Designed Layout and Controls
RT² Profiler PCR Arrays are available in 96-well plate, 384-well plate, and 100-well disc formats, and are used to
monitor the expression of 84 or 370 genes related to a disease state or pathway plus
five housekeeping genes. Controls are also included on each array for genomic
DNA contamination, RNA quality, and general PCR performance. Click [ here
] to learn how the control features work.
Download an easy-to-use Excel-based data analysis template [
here ]. Data analysis is based on the
ΔΔCt method with normalization of the raw data to either housekeeping genes or
an external RNA control.
You can easily perform
a PCR Array experiment in your own laboratory, or send your samples to us and
take advantage of our PCR Array Services.
Presentations and Publications