SureSilencing shRNA Plasmid for Rat Tnfsf10

Gene Informtion

Description: Tumor necrosis factor (ligand) superfamily, member 10  Detailed info member of the tumor necrosis factor-alpha family of cytokines; may induce apoptosis [RGD] Gene Symbol: Tnfsf10 UniGene #: Rn.83627 Refseq Accession #: NM_145681 The RefSeq Accession number refers to the representative sequence used to design the enclosed shRNA.

Description

The SureSilencing™ shRNA Plasmids are designed to specifically knock down the expression of individual genes by RNA interference under either transient (with GFP) or stable transfection (for hygromycin, neomycin or puromycin-resistance) conditions after performance of the appropriate enrichment or selection procedures, respectively. Each vector contains the shRNA under control of the U1 promoter and either the GFP gene, for the enrichment of transiently transfected cells, or the neomycin or puromycin resistance genes, for the selection of stably transfected cells.

Useful Links

Pricing and Ordering qPCR Primer Slide Presentation User Manual White Paper Citations

Protocol

First time users, please refer to the complete protocol in the SureSilencing™ shRNA Plasmid User Manual. Transform 1 µl provided plasmids separately into 50 µl competent E. coli cells according to manufacturer's instructions. Pick colony for large-scale overnight bacterial culture. Prepare stock of high-quality transfection-grade plasmid using a purification kit according to manufacturer's instructions. Transfect plasmids separately into replicate wells of your cell line of interest using conditions previously optimized for the transfection of plasmids into your cell of interest. Enrich the transfected cell population by FACS for GFP or by selection for neomycin or puromycin resistance. Assay the extent of knock-down by real-time RT-PCR in triplicate for each transfection. Perform your biochemical, cell or molecular biological gene function assay.

Materials Included

Please check the kit components immediately after you receive this package. SABiosciences is only responsible for missing items reported within two (2) business days of receipt. Kit Contents: Pre-designed shRNA Plasmids include four tubes each containing individual shRNA Plasmids specific for the same gene. A fifth tube contains a negative control shRNA Plasmid. All plasmids provided are at concentration of 30 ng/µl - 50 ng/µl         Catalog Numbers ending in "G" contain plasmids with the GFP marker.         Catalog Numbers ending in "H" contain plasmids with Hygromycin-resistance marker.         Catalog Numbers ending in "N" contain plasmids with the Neomycin-resistance marker.         Catalog Numbers ending in "P" contain plasmids with the Puromycin-resistance marker. Storage Conditions: The SureSilencing™ shRNA Plasmids are shipped on dry ice or cold packs. Store all tubes at -20 ºC. This product is made under license from The Carnegie Institution of Washington. However, the purchase of this material by a non-academic or for-profit organization will require a license to use the material from Carnegie. License queries may be directed to Gloria Brienza or Gary Kowalczyk at The Carnegie Institution of Washington, 1530 P Street NW, Washington, DC 20005. This product contains proprietary technology owned and/or licensed exclusively for certain fields of use by Promega Corporation (US Patents 7,413,874 & 7,291,711 &/or US Patent Applications 10/564,020, 09/645,706 & 10/314,827). Except as provided herein below, use of this product is restricted to the use by the purchaser of this product in the purchaser’s own research. Such researchers may transfer modified derivatives and modified progeny of this product to their research colleagues who agree in writing to be bound by the terms and conditions of this label license. No transfer of unmodified product is authorized. No other use or transfer of this product or its derivatives is authorized without the express, written consent of each of Promega Corporation and SABiosciences Corporation. Such prohibition includes, without limitation, any and all non-research uses of this product in any procedure, application, product manufacture, or product transfer for which monetary or other consideration is received from another party. With respect to such commercial use, or any therapeutic or diagnostic uses, please contact Promega Corporation for supply and licensing information.