RT² Profiler PCR Array Update
May 28, 2012
We are pleased to announce that we have upgraded several features of the RT²
Profiler PCR Array system. These upgrades were designed to enhance performance
and reliability of this very popular gene expression analysis system.
As of May 28, 2012, the following changes have been made to the RT² Profiler
PCR Array system. Rest assured, for those of you who have started experiments
with our older system, those products will be available for at least six months
from the launch of our upgraded system. However, we anticipate that when you do
start new experiments, or should you choose to integrate the upgraded PCR Arrays
into current experiments, that you will appreciate the improvements we have made
to the system.
Upgrades to the RT² Profiler PCR Arrays (effective
May 28, 2012)
- Pathway Content
The content for all pathways come from literature, including data from
previously published research. As research over the past several years has
shed light on new and exciting developments in the realm of different
biological situations, we have made a concerted effort to update our pathway
content to better reflect the most recent findings. Essentially, better
science = better content.
- Species Specific Housekeeping Gene (HKG) selection
Our original selection of Housekeeping Genes for the PCR Arrays reflected
the then current understanding of gene expression from a comprehensive
review of published data.
As research methodologies continue to improve, the field of gene
expression analysis has spread to more areas of research, and findings
in some of the experimental systems show potential variation in the
expression of genes that had been widely used as housekeeping genes used
to normalize data. The improved RT² Profiler PCR Array system includes
HKGs that have been selected on more stringent Quality Control criteria,
which provides for genes that are consistently expressed across a
greater range of sample types, experimental conditions, and replicates.
- Assay Design Algorithm
Based our extensive experience working with thousands of primer assays, we
have improved our assay design algorithm, taking considerations of RNA
secondary structures and updated SNP information, to further increase the
detection sensitivity of our assays.
- Inert Dye
An inert dye has been added to every well of the RT² Profiler PCR Array. This dye improves our manufacturing quality control, but does not interfere with assay performance.
We will continue to manufacture the existing RT² Profiler PCR Array products
through December 1, 2012, but we encourage researchers to move to the updated
RT² Profiler PCR Arrays. For researchers who are in the middle of important
research experiments, we will continue to support you as you complete your
study. We do ask that you contact our applications scientists to discuss your
projects and how we can help you transition to the updated RT² Profiler PCR
Please contact our Application Scientists with any questions at:
Shankar Sellappan, Ph.D.
Global Product Manager