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Custom EpiTect ChIP PCR Arrays

SABiosciences' Custom ChIP PCR Arrays are the first genome-wide, laboratory-validated, qPCR tool to analyze any promoter region in human, mouse and rat samples. Analyze multiple genomic loci and samples at one time using Custom ChIP PCR Arrays in any 96 or 384 well real-time PCR Instrument.

  • Laboratory-Validated qPCR Design for any 96 or 384 well qPCR Instrument
  • Quickly validate ChIP-ChIP or ChIP-Seq experiments using qPCR
  • Explore multiple transcription factor binding sites in one experiment
  • Investigate the entire Chromatin Structure in one gene around a single transcription start site

 
HOW TO ORDER HOW IT WORKS APPLICATION DATA RESOURCES
 
How to Order
  1. Click the Layout Picture from the choices below to download a Custom ChIP PCR Array excel template. If you would like a different format please contact our ChIP application specialists at 1-888-503-3187 for help.
  2. Populate your Excel Spreadsheet with individual ChIP qPCR Assays. Use the ChIP search portal to find your transcription factor binding site, chromosomal region or Gene of Interest.
  3. Call our ChIP Application Scientists at 1-888-503-3187 to confirm your order and upload your Custom ChIP Excel File to our secure server.

Upon receiving the Excel file with your gene list, your Personalized ChIP Application Scientist will contact you to confirm your Custom ChIP PCR Array design and provide a price quote. Custom PCR Arrays are manufactured, laboratory-verified, and will be shipped in 2-3 weeks from order verification.

PCR Array Plate Format

96 Well Formats 384 Well Formats

12 Loci / 8 Samples

16 Loci / 24 Samples

24 Loci / 4 Samples

24 Loci / 16 Samples

32 Loci / 3 Samples

32 Loci / 12 Samples

48 Loci / 2 Samples

96 Loci / 4 Samples

96 Loci / 1 Samples

384 Loci / 1 Samples
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HOW TO ORDER HOW IT WORKS APPLICATION DATA RESOURCES
 

The ChIP PCR array is a set of optimized real-time PCR primer assays on 96-well or 384-well plates for pathway or disease focused analysis of in vivo protein-DNA interactions. The ChIP PCR array performs ChIP DNA analysis with real-time PCR sensitivity and the multi-genomic loci profiling capability of a ChIP-on-chip. Simply mix your ChIP DNA samples with the appropriate ready-to-use PCR master mix, aliquot equal volumes to each well of the same plate, and then run the real-time PCR cycling program.

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HOW TO ORDER HOW IT WORKS APPLICATION DATA RESOURCES
  Cancer Research

Signaling pathway activation of transcription factors (TFs) can occur by a variety of mechanisms. Anti-cancer agents, such as 5-fluorouracil (5FU), have been shown to induce apoptosis by activation of TFs such as the tumor suppressor protein, p53. The p53 protein activation induces a site-specific binding event that allows coordination of p53 interactions with chromatin and transcriptional complexes at the promoter region of a target gene. These interactions are often cell-type and tissue-specific. EpiTect ChIP PCR Arrays were used to examine the cell type-specific p53 occupancy before and after 5FU treatment, on the promoter region of a panel of genes implicated in apoptosis and cell cycle regulation. Three cancer cell lines with wild-type p53 protein (A549, HepG2, MCF7 cells) and with functional mutant p53 protein (PC3 cells) were tested.

Figure 1: A Model for Stimuli-Specific Regulation or Tissue/Cell Type-Specific Response to Drug Treatment.

Figure 2: Association between Transcription Binding Site Occupancy and Differential Expression. Four different cell lines treated and untreated with 5FU were subjected to either; ChIP assay using EpiTect ChIP One-Day Kit and EpiTect ChIP p53 Antibody Kit or gene expression analysis using RT² p53 Signaling Pathway PCR Arrays. The results from both types of Arrays were represented as the fold change upon 5FU treatment. Comparison of the fold changes in both gene expression levels and p53-binding site occupancy implies a coordinated cell type-specific and stimuli-specific gene regulation by p53. The results show that the ChIP PCR Arrays is a powerful tool in studying complex pathway signals affecting gene transcription.

EpiTect ChIP PCR Arrays can be used to monitor differential histone modifications across a gene.

Figure 3: The Custom EpiTect ChIP Promoter Binding Array Quickly Maps Histone Modifications Surrounding the Transcription Start Site (TSS) of the CDKN1A Gene. EpiTect ChIP Antibodies against modified histones (H3Ac, H3K4me2, H3K27me3), or NIS (non-immune serum)were used to precipitate chromatin from one million HeLa cells. Each ChIP DNA fraction was analyzed with a Custom EpiTect ChIP Promoter Binding Array representing 30 one-kb tile intervals across the promoter region of the CDKN1A gene. The results indicate the enrichment of histone markers for actively transcribed genes (H3Ac and H3K4me2) but not histone markers for transcriptional inactive genes (H3K27me3) in the genomic region surrounding the TSS of CDNK1A.

 

HOW TO ORDER HOW IT WORKS APPLICATION DATA RESOURCES
 
User Manual EpiTect ChIP PCR Array User Manual (PDF)
Data Analysis Free ChIP PCR Array Data Analysis Software (Excel based)
Other Needed Materials Accessories, Equipment & Reagents Required for ChIP PCR Array
Web Seminars Attend a live on-line seminar on EpiTect ChIP PCR System
EpiTect ChIP One-Day Kit: (334471)Sufficient for 12 Chromatin Immunoprecipitations & DNA Purifications
RTē SYBR Green qPCR Master Mix Match with your instrument, 200-reaction scaleOrder one mix for each pair of 96-well or each individual 384-well PCR Array
EpiTect ChIP Antibody Kit Experimentally validated ChIP-grade antibody of interest, control IgG, and control PCR primer sets are included.
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