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Home > Products and Services > ChIP-qPCR > Custom EpiTect ChIP PCR Arrays
Custom EpiTect ChIP PCR Arrays
SABiosciences' Custom ChIP PCR Arrays are the first genome-wide,
laboratory-validated, qPCR tool to analyze any promoter region in human, mouse
and rat samples. Analyze multiple genomic loci and samples at one time using
Custom ChIP PCR Arrays in any 96 or 384 well real-time PCR Instrument.
- Laboratory-Validated qPCR Design for any 96 or 384 well qPCR Instrument
- Quickly validate ChIP-ChIP or ChIP-Seq experiments using qPCR
- Explore multiple transcription factor binding sites in one experiment
- Investigate the entire Chromatin Structure in one gene around a single
transcription start site
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The ChIP PCR array is a set of optimized real-time PCR primer assays on 96-well
or 384-well plates for pathway or disease focused analysis of in vivo
protein-DNA interactions. The ChIP PCR array performs ChIP DNA analysis with
real-time PCR sensitivity and the multi-genomic loci profiling capability of a
ChIP-on-chip. Simply mix your ChIP DNA samples with the appropriate ready-to-use
PCR master mix, aliquot equal volumes to each well of the same plate, and then
run the real-time PCR cycling program.
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Cancer Research
Signaling pathway activation of transcription factors (TFs) can occur by a
variety of mechanisms. Anti-cancer agents, such as 5-fluorouracil (5FU), have
been shown to induce apoptosis by activation of TFs such as the tumor
suppressor protein, p53. The p53 protein activation induces a site-specific
binding event that allows coordination of p53 interactions with chromatin and
transcriptional complexes at the promoter region of a target gene. These
interactions are often cell-type and tissue-specific. EpiTect ChIP PCR Arrays
were used to examine the cell type-specific p53 occupancy before and after 5FU
treatment, on the promoter region of a panel of genes implicated in apoptosis
and cell cycle regulation. Three cancer cell lines with wild-type p53 protein
(A549, HepG2, MCF7 cells) and with functional mutant p53 protein (PC3 cells)
were tested.
| Figure 1: |
A Model for Stimuli-Specific
Regulation or Tissue/Cell Type-Specific Response to Drug Treatment.
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| Figure 2: |
Association between Transcription
Binding Site Occupancy and Differential Expression. Four different cell
lines treated and untreated with 5FU were subjected to either; ChIP
assay using EpiTect ChIP
One-Day Kit and EpiTect ChIP
p53 Antibody Kit or gene expression analysis using RT²
p53 Signaling Pathway PCR Arrays. The results from both types of
Arrays were represented as the fold change upon 5FU treatment.
Comparison of the fold changes in both gene expression levels and
p53-binding site occupancy implies a coordinated cell type-specific and
stimuli-specific gene regulation by p53. The results show that the ChIP PCR
Arrays is a powerful tool in studying complex pathway signals affecting
gene transcription. |
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EpiTect ChIP PCR Arrays can be used to monitor differential
histone modifications across a gene.
| Figure 3: |
The Custom EpiTect ChIP
Promoter Binding Array Quickly Maps Histone Modifications Surrounding
the Transcription Start Site (TSS) of the CDKN1A Gene. EpiTect ChIP
Antibodies against modified histones (H3Ac, H3K4me2, H3K27me3), or NIS
(non-immune serum)were used to precipitate chromatin from one million
HeLa cells. Each ChIP DNA fraction was analyzed with a Custom
EpiTect ChIP Promoter Binding Array representing 30 one-kb tile
intervals across the promoter region of the CDKN1A gene. The results
indicate the enrichment of histone markers for actively transcribed
genes (H3Ac and H3K4me2) but not histone markers for transcriptional
inactive genes (H3K27me3) in the genomic region surrounding the TSS of
CDNK1A. |
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Complete Array List
