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Chromatin IP (ChIP) and qPCR Assay System
The EpiTect ChIP qPCR System provides a complete platform for the in
vivo analysis of the dynamic interaction between DNA and nuclear proteins that
play a central role in gene regulation. This technology is the first integrated
system in the world for chromatin IP DNA preparation and real-time PCR analysis.
How it works
The EpiTect ChIP PCR Array is a panel of 96 primer assays
for pathway or disease-focused genes in a 96-well or 384-well
plate. These primer assays are designed and optimized for the
pathway-focused analysis of in vivo protein-DNA interactions.
The EpiTect ChIP PCR array performs ChIP DNA analysis with
real-time PCR sensitivity and the multi-genomic loci
profiling capability of ChIP-on-chip. Simply mix your ChIP
DNA sample with the appropriate RT² SYBR™ Green PCR master
mix, aliquot equal volumes into each well of the array plate,
and then run the real-time PCR cycling program. Following
real-time PCR, analyze your data using the free, downloadable
Excel template.
WHY USE THE EpiTect ChIP
qPCR SYSTEM?
- Complete Solution:
Standardized system from chromatin IP to qPCR including Data Analysis
- Scalable:
Focus on one region with ChIP qPCR Assays or multiple regions with ChIP
PCR Arrays
- Fast and Accurate
From Sample Isolation to Fold Change Results in 1 to 2 days.
- EpiTect ChIP PCR Array
Pathway-focused analysis of DNA-histone, DNA-transcription factors, and
other DNA-protein interactions, choose from catalog pathways or create a
custom array
- EpiTect ChIP One-Day Kit
Streamlined, optimized, 6-hour ChIP protocol with a high
yield of high quality DNA
- EpiTect ChIP Antibody Kits
Includes experimentally validated ChIP-grade antibodies with
control IgG and positive and negative control PCR primer
sets
- EpiTect ChIP PCR Primers
Experimentally validated genome-wide qPCR primers
specifically designed for the analysis of ChIP-enriched
genomic DNA
- ChIP PCR Data Analysis
Free Excel-based tool for conversion of your raw Ct values
into easy to use and practical readouts of percentage of
input and fold difference
- EpiTect ChIP Search Portal
Free, easy-to-use Web tool to find potential regulators of
your gene of interest and PCR primers for the associated
promoter binding sites by searching gene name, transcription
factor, or chromosomal position
- RT² SYBR™ Green PCR Mastermix
Ready-to-use and validated for ChIP DNA analysis, available with and without
reference dye.
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