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miRNA PCR Array Service

 
PCR Arrays miRNA PCR Arrays SYBR Single Gene Assays
 
SABiosciences service core offers miRNA (microRNA) PCR array and assay services based on the miScript miRNA PCR Array technology. These arrays and assays employ SABiosciences' proprietary miRNA technology that ingeniously integrates a universal tailing and reverse transcription reaction specific for miRNA with the accurate expression level measurement of distinct miRNA sequences that may only differ by a single nucleotide base. In addition, this miRNA detection technology enables uniformly high PCR amplification efficiencies, allowing simultaneous detection of miRNA under uniform cycling conditions. With these technologies, either a comprehensive survey of miRNA expression can easily be obtained, or the expression level of individual miRNAs can be measured. SABiosciences' validated procedures and optimized processes yield the highest data quality and fastest turn-around time.

For FFPE (formalin-fixed paraffin-embedded) samples, LCM (laser capture microdissection), FNAB (fine needle aspiration biopsies) and other small samples, require special isolation.

Description of services

miRNA isolation or enrichment service (optional)

miRNA PCR service
Accurately analyzing the expression of multiple microRNA sequences at the same time with high sensitivity and specificity using:

Catalogued miRNA PCR arrays

Individual miRNA PCR Assays

Data analysis

 

Sample Processing Data Delivery Pricing
 

Submitted Samples are Cells or Tissue:
     Option of RNA isolation service
     Recommended Sample Isolation and Storage Procedure

Submitted Samples are Total RNA:
     Recommended RNA Isolation methods
     Quantification and estimating RNA purity using a NanoDrop spectrophotometer
     Assessing RNA quality using an Agilent 2100 Bioanalyzer with a RNA 6000 NanoChip

Reverse-transcription
Real-time detection on cataloged or custom miRNA PCR array or individual miRNA assays
Data analysis using SABiosciences' data analysis software
Data report delivered via email or secure FTP site

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Sample Processing Data Delivery Pricing
  Data analysis is performed using SABiosciences qPCR data analysis software. Data in an electronic form can be downloaded from a secure FTP website or delivered via email. A complete data and experiment report includes:

Raw Ct values
ΔΔCt Analysis
Excel spreadsheet (data analysis using pair-wise comparisons, including raw data, tabular results as well as Scatter, 3D, and Volcano plots)

View a sample data set (.PDF) (email: support@sabiosciences.com)

Custom data format service is also available.

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Sample Processing Data Delivery Pricing
 

Contact a Technical Support representative to discuss your specific needs.
For complete details or to get a specific quotation, please contact a Technical Support representative at support@sabiosciences.com or 301-682-9200

Prices include the cost of arrays and reagents. Service prices are volume-dependent. Progressive discounts for fees and services are available. All prices are in US Dollars. Prices are subject to change at any time without notice.

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