NOTE: To access Data Analysis templates of the original EpiTect Methyl qPCR Arrays (Before May 25, 2012), please click here.
Please NOTE: The new EpiTect Methyl II PCR Array System was introduced effective May 25, 2012. It has replaced the EpiTect Methyl qPCR Array System, with the older system set for discontinuation December 1, 2012.
These integrated Excel-based templates automatically perform all ΔCt based calculations from your raw threshold cycle (Ct) values to determine gene specific DNA methylation status, using MethylScreen™ technology. The Excel template normalizes the Ct values of both digests with the mock digestion values to calculate and report the percentage of the DNA that is methylated and unmethylated.
Quickly Understand the Biology:
Results automatically calculated, analyzed, and annotated
No Software to download, license or install:
Simply paste in your raw Ct values
EpiTect Methyl II PCR Arrays provide gene methylation status as percentage
unmethylated (UM) and percentage methylated (M) fraction of input DNA.
"UM" represents the fraction of input genomic DNA containing no
methylated CpG sites in the amplified region of a gene. "M" represents
the fraction of input genomic DNA containing two or more methylated CpG sites in
the targeted region of a gene. The number of CpG sites methylated in a targeted
region can vary within the fraction of methylated input DNA.
In the figure above, each horizontal bar represents the targeted region of a gene from one genome. Biological samples usually contain many genomes derived from many cell types. For simplicity, five such genomes are depicted. Light and dark circles represent unmethylated and methylated CpG site, respectively.
Find the Correct Template for Your Array:
Use the pull down menu to select your EpiTect Methyl II PCR Array
Download the correct version of the template specific for your plate