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EpiTect Methyl II PCR Data Analysis

NOTE: To access Data Analysis templates of the original EpiTect Methyl qPCR Arrays (Before May 25, 2012), please click here. Please NOTE: The new EpiTect Methyl II PCR Array System was introduced effective May 25, 2012. It has replaced the EpiTect Methyl qPCR Array System, with the older system set for discontinuation December 1, 2012.

These integrated Excel-based templates automatically perform all ΔCt based calculations from your raw threshold cycle (Ct) values to determine gene specific DNA methylation status, using MethylScreen™ technology. The Excel template normalizes the Ct values of both digests with the mock digestion values to calculate and report the percentage of the DNA that is methylated and unmethylated.

Benefits:

  • Quickly Understand the Biology:
    Results automatically calculated, analyzed, and annotated
  • No Software to download, license or install:
    Simply paste in your raw Ct values

Data Interpretation
EpiTect Methyl II PCR Arrays provide gene methylation status as percentage unmethylated (UM) and percentage methylated (M) fraction of input DNA. "UM" represents the fraction of input genomic DNA containing no methylated CpG sites in the amplified region of a gene. "M" represents the fraction of input genomic DNA containing two or more methylated CpG sites in the targeted region of a gene. The number of CpG sites methylated in a targeted region can vary within the fraction of methylated input DNA.

In the figure above, each horizontal bar represents the targeted region of a gene from one genome. Biological samples usually contain many genomes derived from many cell types. For simplicity, five such genomes are depicted. Light and dark circles represent unmethylated and methylated CpG site, respectively.

Find the Correct Template for Your Array:

  1. Use the pull down menu to select your EpiTect Methyl II PCR Array
  2. Download the correct version of the template specific for your plate layout.
Gene Panel (#) Plate Type (Format)
Signature (22) 96-Well (A, C, D, F)
Signature (22) 384-Well (E, G)
Complete (94) 96-Well (A, C, D, F)
Complete (94) 384-Well (E, G)
EpiTect Methyl II Custom PCR Array  96-Well (A, C, D, F)
EpiTect Methyl II Custom PCR Array  384-Well (E, G)
EpiTect Methyl II PCR Assay All

Graphical Analysis of EpiTect Methyl II Data  NEW!

The EpiTect Methyl II PCR System use the MethylScreen™ Technology provided under license from Orion Genomics, LLC.

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