EpiTect Methyl II PCR Primer Assays

Search EpiTect Methyl II PCR Primers for Your Genes

Note: If you need any information on an EpiTect Methyl qPCR Primer that you purchased prior to May 25, 2012, please call or email our Tech Support.

EpiTect Methyl II PCR Assays are a simple and reliable method for quickly detecting the DNA methylation status of the CpG islands associated with individual genes, using MethylScreen™ technology. QIAGEN has developed cataloged EpiTect Methyl II PCR Primers for analyzing any human, mouse, or rat promoter CpG island.

 Search your EpiTect Methyl II PCR Primers:

*Custom EpiTect Methyl II PCR Primers are available for other CpG loci that are not covered by our cataloged products. Please inquire here.

Benefits:

• Simple & Fast: No bisulfite conversion; Process a large number of samples/genes in a single day.

• Reliable: Our rigorous qPCR primer validation and quality control manufacturing process guarantee reliable assay performance.

• Genome-wide Coverage: Cataloged EpiTect Methyl II PCR Primers are available for human, mouse and rat promoter CpG islands.

How It Works:

Primer Design Principles
Cataloged EpiTect Methyl II PCR Primers are available for more than 37,000 promoter CpG islands, annotated based on the combination of UCSC genome bioinformatics predictions and the CpG islands with published evidence. Each EpiTect Methyl II PCR primer assay corresponds to one distinct CpG island in a promoter region, defined as from 5 kb upstream to 3 kb downstream of transcription start site (TSS). All EpiTect Methyl II PCR primers are first designed by a rigorously optimized computer algorithm that accounts for the GC-rich sequences in genomic DNA and particularly in CpG islands. The design algorithm also insures that every amplicon contains sufficient cutting sites for both methyl-sensitive and methyl-dependent enzymes to maximize methylation detection sensitivity.

Manuals & Resources

EpiTect Methyl II PCR Assay Manual

EpiTect Methyl II DNA Restriction Kit
Required for a complete and successful experiment
Includes enzymes and a digestion buffer optimized for complete digestion by both enzymes under the same conditions

RT² SYBR® Green qPCR Master Mixes
Instrument-specific PCR master mixes insure high-efficiency, sequence-specific amplification

EpiTect Methyl II PCR Arrays
Analyze the DNA Methylation Status of 22 or 94 Genes Simultaneously

Data Analysis Software (Excel template)

The EpiTect Methyl II PCR Arrays use the MethylScreen™ Technology provided under license from Orion Genomics, LLC.