DNA Methylation Related Products
Complement your PCR-based DNA methylation analysis with other experiments
using our pathway-focused and genome-wide gene expression and/or gene function
analysis solutions. Confirm whether changes in the methylation status of genes
affect their expression using real-time RT-PCR. Or explore the function of the
methylated genes of interest with RNA interference and cell-based functional
RT² Profiler™ PCR Arrays & RT?qPCR Primers
Verify that hypermethylating or hypomethylating your gene promoters of interest
decreases or increases their transcript levels, respectively, and/or alters the
expression of other genes in the same biological pathway using real-time RT-PCR.
The RT?qPCR Primers analyze the transcript levels of individual genes, while
the RT?Profiler PCR Arrays profile a panel of pathway-focused or
disease-specific mRNAs. Both methods are optimized for high performance SYBR
Green based qRT-PCR detection.
Get gene expression profiling results even more quickly and easily by letting
our in-house experts perform the real-time RT-PCR or PCR Array analysis for you.
Stay focused on your methylation analyses by leaving your mRNA measurements to
SureSilencing™ shRNA Plasmids & siRNA Arrays
Determine the oncogenic role played by your hypermethylated genes of interest,
or genes in the same of related pathways, by knocking down their expression in a
normal cell culture model. The SureSilencing shRNA Plasmids are designed to
knock down the expression of any gene in the human, mouse, or rat genome by
plasmid-based RNA Interference. The SureSilencing siRNA Arrays allow you to
knock down the expression of 42 genes in the same biological pathway.
Cignal™ & Cignal Lenti Reporter Arrays
Confirm that changes in the methylation patterns signal transduction pathway
genes alter their ability to be activated in a normal cell culture model. Use
Cignal or Cignal Lenti GFP or luciferase based reporter assays to study the
regulation of these cellular signaling pathways at the level of transcription
ELISArray™ Kits, Single- & Multi-Analyte
If your hypermethylated or hypomethylated genes of interest encode for cytokines
or chemokines, verify their changes in expression at the protein level using
standard sandwich ELISA kits.