Learn about the EpiTect Methyl qPCR Arrays Update
We are pleased to announce that we have updated the EpiTect Methyl qPCR
System line of products, QIAGEN's DNA methylation profiling system. The updated
line of products is now branded under the EpiTect Methyl II PCR Array System
The new DNA methylation profiling system includes features that enhance data
reliability and will continue to have all of the advantages that researchers
have come to depend upon: sensitive profiling of DNA methylation using PCR
Arrays and individual DNA methylation assays, and convenient ease-of-use without
any bisulfite conversion of DNA.
As a result of the EpiTect Methyl II PCR Array System upgrade, several
changes have occurred:
- The 5x Digestion Buffer in the DNA restriction kit has been spiked with
two plasmids, which serve as controls for monitoring the efficiency of
restriction enzymes A and B
- Two new control assays have been added to each DNA methylation array to
monitor digestion efficiency by restriction enzymes
- Biological content in DNA methylation arrays have been updated to
accommodate the inclusion of the two control assays.
- Some assays in our databases have been changed to reflect updates to the
human genome database.
- New data analysis modules have been established to incorporate more
stringent quality control criteria for analyzing EpiTect Methyl II PCR
The workflow of the system has not changed. DNA profiling of several targets
simultaneously using this system can still be accomplished within a day without
bisulfite conversion of DNA, but now with increased reliability and
reproducibility, through the inclusion of the 2 control plasmids along with
their respective assays. The data analysis modules have been updated to use
these controls to generate a "Pass" or "Fail" call for the
quality of template used for real-time PCR.
We will continue to support the existing EpiTect Methyl qPCR products through
November 1, 2012, but we encourage researchers to move new DNA methylation
profiling projects to the new EpiTect Methyl II PCR product family. For
researchers who are in the middle of important research products, we will
continue to support you as you complete your study. We do ask that you contact
our applications scientists to discuss your projects and how we can help you
transition to the new EpiTect Methyl II PCR System.
Email Application Scientists: firstname.lastname@example.org
Call Application Scientists: 1-888-503-3187, option 2
We understand that any change in reagents during a research project can be
challenging and we are committed to providing unparalleled technical and
biological support during and after this transition. If you have any question,
comments or concerns, please contact our technical support so we can help with
your DNA methylation detection needs.
To continue to the NEW EpiTect Methyl II PCR System page please click here