QIAGEN Website    Quick Order    Online Seminar    Contact    My Account
Home  >  Products and Services  >  PCR Home  >  Fast SYBR® Green Mastermix

RT² Fast SYBR® Green Mastermix

The RT² Fast SYBR Green qPCR Mastermix have been optimized to use a fast PCR protocol, without compromising specificity or sensitivity, using SYBR Green based detection. The RT² Fast SYBR Green qPCR Mastermix contain all of the reagents and buffers required for fast real-time polymerase chain reactions on instrumentation, including an improved proprietary real-time PCR buffer, a high-performance HotStart DNA Taq polymerase, nucleotides, SYBR Green dye, and if necessary, reference dyes needed to normalize the instruments' optics. Simply add the master mix to PCR tubes or plates along with your template and primers, and you are ready to run your real-time assay.

RT² FAST SYBR Green/ ROX: For all ABI, Eppendorf, QIAGEN Rotor-Gene Q, QIAGEN Rotor-Gene 6000 (Corbett), Stratagene, and TaKaRa instrumentation
Cat. No Size
330620
330622
330623
330621
330629
For 2 RT² Profiler™ PCR Arrays OR 200 25-µl reactions (2.5 ml)
For 12 RT² Profiler™ PCR Arrays
For 24 RT² Profiler™ PCR Arrays
For 4 pack of 384-well PCR Arrays
2000 25-µl reactions (25 ml)
RT² FAST SYBR Green/ Fluorescein: For Bio-Rad iCycler, iQ5, MyiQ, MyiQ2
Cat. No Size
330610
330612
330613
330611
330619
For 2 RT² Profiler™ PCR Arrays OR 200 25-µl
For 12 RT² Profiler™ PCR Arrays
For 24 RT² Profiler™ PCR Arrays
For 4 pack of 384-well PCR Arrays
2000 25-µl reactions (25 ml)
RT² SYBR FAST Green: For instrumentation not requiring reference dye
For Bio-Rad CFX 96/384, Chromo4, Opticon2, Roche LightCycler 480 and all other real-time instruments
Cat. No Size
330600
330602
330603
330601
330609
For 2 RT² Profiler™ PCR Arrays OR 200 25-µl
For 12 RT² Profiler™ PCR Arrays
For 24 RT² Profiler™ PCR Arrays
For 4 pack of 384-well PCR Arrays
2000 25-µl reactions (25 ml)

 
Performance Protocol Components
 

Sharper dissociation curves

Dissociation curves generated by regular cycling SYBR Green Master Mix or RT² FAST SYBR Green Master Mix. The dissociation curve obtained by the RT² FAST SYBR Green Mastermix has lower background and sharper shape compared with the dissociation curve generated by regular cycling SYBR Green Master Mix

Regular PCR Program
Fast PCR Program

PPC (Positive PCR Control designed by SA Biosciences) is amplified on the ABI -7500 qRT-PCR instrument, by Regular Cycling SYBR Green Mastermix or FAST SYBR Green Mastermix, respectively. The PCR protocol for RT² Mastermix is the Standard Mode with regular cycling procedure, while the protocol for RT² Fast Mastermix is the Fast Mode with fast cycling procedure.

Superior Performance

SABiosciences Fast qPCR kit outperforms when compared to another leading fast qPCR kit. The SABiosciences Fast qPCR kit consistently produces lower NTC (non-template control) backgrounds and
and earlier Cts in the presence of template DNA.

The SABiosciences Fast and Competitor Q Fast qPCR master mixes were applied on the same PCR plate to compare their performance side by side on an ABI 7500 qRT-PCR instrument.

Back to Top
 

Performance Protocol Components
 

Ensure that you do not contaminate the 2X real-time PCR cocktail by dividing into aliquots containing the amount of cocktail necessary for the number of reactions you are preparing each day. The rest of the cocktail should be kept in storage away from any sources of template DNA.

When using RT² qPCR Mastermix with RT² Profiler™ PCR Arrays, RT² qPCR Primer Assays, refer to the appropriate User Manuals for reaction mixtures.

Back to Top

 

Performance Protocol Components
 

Please check the kit components immediately after you receive this package. We are not responsible for any missing items not reported within two (2) business days upon receipt.

Contents:

  • Tube Format (Each tube contains 1.4 mL of 2X solution, sufficient for one PCR Array or for 100 25-µL reactions)
    o 330600, 330610, 330620: Two (2) tubes
    o 330602, 330612, 330622: Twelve (12) tubes
    o 330603, 330613, 330623: Twenty-four (24) tubes
    o 330601, 330611, 330621: Eight (8) tubes
  • Bottle Format (25 mL of 2X solution, sufficient for 2000 25-µL reactions)
    o 330609, 330619, 330629: One (1) bottle

Storage Conditions
The kit is shipped on dry ice or cold packs. For long-term storage, keep at -20ºC. If entire volume is not be used all at once, divide into aliquots and store at -20ºC. Avoid repeated freezing and thawing.

Shelf Life
All reagents are stable for 6 months after receipt if stored at the recommended temperature.

NOTICE TO PURCHASER: LIMITED LICENSE

Use of this product is covered by one of more of the following US patents and corresponding patent claims outside the US: 5,079,352, 5,789,224, 5,618,711, 6,127,155, 5,677,152 (claims 1 to 23 only), 5,773,258 (claims 1 and 6 only), 5,407,800, 5,322,770, 5,310,652, 5,994,056, 6,171,785, and claims outside the US corresponding to US Patent No. 4,889,818. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Back to Top