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Home > Products and Services > PCR Home > Fast SYBR® Green Mastermix
RT² Fast SYBR® Green Mastermix
The RT² Fast SYBR Green qPCR Mastermix have been optimized to use a fast PCR protocol, without compromising specificity or sensitivity, using SYBR Green based detection. The RT² Fast SYBR Green qPCR Mastermix contain all of the reagents and buffers required for fast real-time polymerase chain reactions on instrumentation, including an improved proprietary real-time PCR buffer, a high-performance HotStart DNA Taq polymerase, nucleotides, SYBR Green dye, and if necessary, reference dyes needed to normalize the instruments' optics. Simply add the master mix to PCR tubes or plates along with your template and primers, and you are ready to run your real-time assay.
| RT² FAST SYBR Green/ ROX:
For all ABI, Eppendorf, QIAGEN Rotor-Gene Q, QIAGEN Rotor-Gene 6000 (Corbett), Stratagene, and TaKaRa instrumentation |
| Cat. No |
Size |
330620
330622
330623
330621
330629 |
For 2 RT² Profiler™ PCR
Arrays OR 200 25-µl reactions (2.5 ml)
For 12 RT² Profiler™ PCR Arrays
For 24 RT² Profiler™ PCR Arrays
For 4 pack of 384-well PCR Arrays
2000 25-µl reactions (25 ml) |
| RT² FAST SYBR Green/
Fluorescein: For Bio-Rad iCycler, iQ5, MyiQ, MyiQ2 |
| Cat. No |
Size |
330610
330612
330613
330611
330619 |
For 2 RT² Profiler™ PCR
Arrays OR 200 25-µl
For 12 RT² Profiler™ PCR Arrays
For 24 RT² Profiler™ PCR Arrays
For 4 pack of 384-well PCR Arrays
2000 25-µl reactions (25 ml) |
RT² SYBR FAST Green:
For instrumentation not requiring reference dye
For Bio-Rad CFX 96/384, Chromo4, Opticon2, Roche LightCycler 480 and
all other real-time instruments |
| Cat. No |
Size |
330600
330602
330603
330601
330609 |
For 2 RT² Profiler™ PCR
Arrays OR 200 25-µl
For 12 RT² Profiler™ PCR Arrays
For 24 RT² Profiler™ PCR Arrays
For 4 pack of 384-well PCR Arrays
2000 25-µl reactions (25 ml) |
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Sharper dissociation curves
Dissociation curves generated by regular cycling SYBR Green Master Mix or RT² FAST SYBR Green Master Mix. The dissociation curve
obtained by the RT² FAST SYBR Green Mastermix has lower background and sharper
shape compared with the dissociation curve generated by regular cycling SYBR
Green Master Mix
Regular PCR Program
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Fast PCR Program
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PPC (Positive PCR Control designed by SA Biosciences) is amplified on the
ABI -7500 qRT-PCR instrument, by Regular Cycling SYBR Green Mastermix
or FAST SYBR Green Mastermix, respectively. The PCR protocol for
RT² Mastermix is the Standard Mode with regular cycling procedure, while the protocol
for RT² Fast Mastermix is the Fast Mode with fast cycling procedure.
Superior Performance
SABiosciences Fast qPCR kit outperforms when compared to another leading fast
qPCR kit. The SABiosciences Fast qPCR kit consistently produces lower NTC
(non-template control) backgrounds and
and earlier Cts in the presence of template DNA.
The SABiosciences Fast and Competitor Q Fast qPCR master
mixes were applied on the same PCR plate to compare their performance side by
side on an ABI 7500 qRT-PCR instrument.
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- Ensure that you do not contaminate the 2X real-time PCR cocktail by
dividing into aliquots containing the amount of cocktail necessary for
the number of reactions you are preparing each day. The rest of the
cocktail should be kept in storage away from any sources of template
DNA.
- For general real-time PCR purposes, mix the following components in a
PCR tube:
12.5 µl RT² Fast SYBR® Green qPCR Mastermix
10.5 µl ddH2O
1.0 µl template cDNA (up to 250 ng)
1.0 µl gene-specific 10 µM PCR primer pair stock
25.0 µl final volume
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Recommended real-time thermal cycler program:
NOTE: The 10 min step at 95 ºC is required to activate the
HotStart Taq DNA polymerase.
95 ºC, 10 min; 40 cycles of (95 ºC, 10 sec;
60 ºC, 30 sec).
For ABI 7500, select the "Fast 7500" in the Run Mode panel.
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Program the real-time thermal cycler to detect and record the SYBR® Green
signal from every reaction at the end of the 60ºC annealing /
extension step of each cycle.
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Run your instrument's default melting curve program immediately after the
above PCR program, and generate a first derivative dissociation curve. No
more than one peak should appear in each reaction
If your instrument does not have a default melting curve program, run
the following program instead:
95ºC, 1 min; 65ºC, 2 min (OPTICS OFF); 65ºC to 95ºC
at 2ºC / min (OPTICS ON).
NOTE: Be sure to visually inspect the plate after the run for any signs
of evaporation from any of the wells. If evaporation is observed, make a note of
which wells so that you may qualify your data analysis appropriately.
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Please check the kit components immediately after you receive this package.
We are not responsible for any missing items not reported within two (2)
business days upon receipt.
Contents:
- Tube Format (Each tube contains 1.4 mL of 2X solution, sufficient for one
PCR Array or for 100 25-µL reactions)
o 330600, 330610, 330620: Two (2) tubes
o 330602, 330612, 330622: Twelve (12) tubes
o 330603, 330613, 330623: Twenty-four (24) tubes
o 330601, 330611, 330621: Eight (8) tubes
- Bottle Format (25 mL of 2X solution, sufficient for 2000 25-µL reactions)
o 330609, 330619, 330629: One (1) bottle
Storage Conditions
The kit is shipped on dry ice or cold packs. For long-term storage, keep at -20ºC.
If entire volume is not be used all at once, divide into aliquots and store at
-20ºC. Avoid repeated freezing and thawing.
Shelf Life
All reagents are stable for 6 months after receipt if stored at the recommended
temperature.
NOTICE TO PURCHASER: LIMITED LICENSE
Use of this product is covered by one of more of the following
US patents and corresponding patent claims outside the US: 5,079,352, 5,789,224,
5,618,711, 6,127,155, 5,677,152 (claims 1 to 23 only), 5,773,258 (claims 1 and 6
only), 5,407,800, 5,322,770, 5,310,652, 5,994,056, 6,171,785, and claims outside
the US corresponding to US Patent No. 4,889,818. The purchase of this product
includes a limited, non-transferable immunity from suit under the foregoing
patent claims for using only this amount of product for the purchaser's own
internal research. No right under any other patent claim (such as apparatus or
system claims in US Patent No. 6,814,934) and no right to perform commercial
services of any kind, including without limitation reporting the results of
purchaser's activities for a fee or other commercial consideration, is conveyed
expressly, by implication, or by estoppel. This product is for research use
only. Diagnostic uses under Roche patents require a separate license from Roche.
Further information on purchasing licenses may be obtained by contacting the
Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster
City, California 94404, USA.
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