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RT² qPCR Mastermix

Benefits of SABiosciences' SYBR® Green qPCR Mastermix:

Accuracy: Guarantees single gene-specific products with minimal primer dimers.
Reliability: Maximizes amplification efficiencies.
Convenience: Specially formulated all-in-one mixes that match the instrument in your lab.

To learn more about their performance, see our SYBR Green Master Mix Brochure.

Find the qPCR master mix for the instrument available in your lab:
RT² SYBR Green/ ROX: For all ABI, Eppendorf, Stratagene, and TaKaRa instrumentation
Cat. No. Size
330520
330522
330523
330521
330529
For 2 RT² Profiler™ PCR Arrays OR 200 25-µl reactions 
For 12 RT² Profiler™ PCR Arrays
For 24 RT² Profiler™ PCR Arrays
For 4 pack of 384-well PCR Arrays
2000 25-µl reactions (25 ml)
RT² SYBR Green/ Fluorescein: For Bio-Rad iCycler, iQ5, MyiQ, MyiQ2
Cat. No. Size
330510
330512
330513
330511
330519
For 2 RT² Profiler™ PCR Arrays OR 200 25-µl reactions 
For 12 RT² Profiler™ PCR Arrays
For 24 RT² Profiler™ PCR Arrays
For 4 pack of 384-well PCR Arrays
2000 25-µl reactions (25 ml)
RT² SYBR Green: For instrumentation not requiring reference dye
For Bio-Rad CFX 96/384, Chromo4, Opticon2, Roche LightCycler 480 and all other real-time instruments
Cat. No. Size
330500
330502
330503
330501
330509
For 2 RT² Profiler™ PCR Arrays OR 200 25-µl reactions
For 12 RT² Profiler™ PCR Arrays
For 24 RT² Profiler™ PCR Arrays
For 4 pack of 384-well PCR Arrays
2000 25-µl reactions (25 ml)

Master Mix quality plays a critical role in the performance

The RT² SYBR® Green qPCR Mastermix contain all of the optimized reagents and buffers needed for SYBR® Green based real-time polymerase chain reactions in all real-time PCR instrumentation

RT² SYBR® Green qPCR Mastermix, from the experts in high-performance quantitative PCR, provide:

Accuracy: Guaranteed single gene-specific products with minimal primer dimers
Reliability: Maximized amplification efficiencies
Convenience: Matched to the instrument in your lab with specially formulated all-in-one mixes

 
Performance Protocol Components
 

High Amplification Efficiency over a Wide Dynamic Range:

Brighter SYBR Green Signal Means Greater Sensitivity:

Tighter Control of Polymerase Activity Yields Greater Specificity:

SABiosciences Master Mix

Competitor Master Mix

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Performance Protocol Components
 

Ensure that you do not contaminate the 2X real-time PCR cocktail by dividing into aliquots containing the amount of cocktail necessary for the number of reactions you are preparing each day. The rest of the cocktail should be kept in storage away from any sources of template DNA.

When using RT² qPCR Mastermix with RT² Profiler™ PCR Arrays, RT² qPCR Primer Assays, refer to the appropriate User Manuals for reaction mixtures.

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Performance Protocol Components
 

Please check the kit components immediately after you receive this package. We are not responsible for any missing items not reported within two (2) business days upon receipt.

Contents:

330500, 330510, 330520: Two (2) tubes
330502, 330512, 330522: Twelve (12) tubes
330503, 330513, 330523: Twenty-four (24) tubes
330501, 330511, 330521: Eight (8) tubes

Each tube contains 1.25 mL of 2X solution, sufficient for one PCR Array or for 100 25-µL reactions

330509, 330519, 330529: One (1) bottle

Bottle contains 25 mL of 2X solution, sufficient for 2000 25-µL reactions

NOTICE TO PURCHASER: LIMITED LICENSE

Use of this product is covered by one of more of the following US patents and corresponding patent claims outside the US: 5,079,352, 5,789,224, 5,618,711, 6,127,155, 5,677,152 (claims 1 to 23 only), 5,773,258 (claims 1 and 6 only), 5,407,800, 5,322,770, 5,310,652, 5,994,056, 6,171,785, and claims outside the US corresponding to US Patent No. 4,889,818. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Storage Conditions:
The kit is shipped on dry ice or cold packs. For long-term storage, keep at -20 ºC. If entire volume is not be used all at once, divide into aliquots and store at -20 ºC. Avoid repeated freezing and thawing.

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