RT² qPCR Mastermix

Accuracy: Guarantees single gene-specific products with minimal primer dimers.
Reliability: Maximizes amplification efficiencies.
Convenience: Specially formulated all-in-one mixes that match the instrument in your lab.

To learn more about their performance, see our SYBR Green Master Mix Brochure.

Find the qPCR master mix for the instrument available in your lab:

RT² SYBR Green/ ROX: For all ABI, Eppendorf, Stratagene, and TaKaRa instrumentation
Cat. No.	Size
330520 330522 330523 330521 330529	For 2 RT² Profiler™ PCR Arrays OR 200 25-µl reactions  For 12 RT² Profiler™ PCR Arrays For 24 RT² Profiler™ PCR Arrays For 4 pack of 384-well PCR Arrays 2000 25-µl reactions (25 ml)
RT² SYBR Green/ Fluorescein: For Bio-Rad iCycler, iQ5, MyiQ, MyiQ2
Cat. No.	Size
330510 330512 330513 330511 330519	For 2 RT² Profiler™ PCR Arrays OR 200 25-µl reactions  For 12 RT² Profiler™ PCR Arrays For 24 RT² Profiler™ PCR Arrays For 4 pack of 384-well PCR Arrays 2000 25-µl reactions (25 ml)
RT² SYBR Green: For instrumentation not requiring reference dye For Bio-Rad CFX 96/384, Chromo4, Opticon2, Roche LightCycler 480 and all other real-time instruments
Cat. No.	Size
330500 330502 330503 330501 330509	For 2 RT² Profiler™ PCR Arrays OR 200 25-µl reactions For 12 RT² Profiler™ PCR Arrays For 24 RT² Profiler™ PCR Arrays For 4 pack of 384-well PCR Arrays 2000 25-µl reactions (25 ml)

Master Mix quality plays a critical role in the performance

The RT² SYBR® Green qPCR Mastermix contain all of the optimized reagents and buffers needed for SYBR® Green based real-time polymerase chain reactions in all real-time PCR instrumentation

RT² SYBR® Green qPCR Mastermix, from the experts in high-performance quantitative PCR, provide:

Accuracy: Guaranteed single gene-specific products with minimal primer dimers
Reliability: Maximized amplification efficiencies
Convenience: Matched to the instrument in your lab with specially formulated all-in-one mixes

　

Performance

Protocol

Components

High Amplification Efficiency over a Wide Dynamic Range: Brighter SYBR Green Signal Means Greater Sensitivity: Tighter Control of Polymerase Activity Yields Greater Specificity: SABiosciences Master Mix Competitor Master Mix Back to Top

Performance

Protocol

Components

Ensure that you do not contaminate the 2X real-time PCR cocktail by dividing into aliquots containing the amount of cocktail necessary for the number of reactions you are preparing each day. The rest of the cocktail should be kept in storage away from any sources of template DNA. When using RT² qPCR Mastermix with RT² Profiler™ PCR Arrays, RT² qPCR Primer Assays, EpiTect Methyl PCR Arrays, DNA Methylation qPCR Assays, RT² miRNA PCR Arrays, RT² miRNA qPCR Assays, or EpiTect ChIP PCR Arrays & Real-time ChIP-qPCR Primer Assays, refer to the appropriate User Manuals for reaction mixtures. For general PCR purposes, mix the following components in a PCR tube: 12.5 µl RT² SYBR® Green qPCR Mastermix 10.5 µl ddH2O 1.0 µl template cDNA (up to 250 ng) 1.0 µl gene-specific 10 µM PCR primer pair stock 25.0 µl final volume Recommended real-time thermal cycler program. Please find your product for the correct cycling conditions. *NOTE: The 10 min step at 95 ºC is required to activate the HotStart Taq DNA polymerase. RT² Profiler PCR Arrays & RT² Primer Assays Select the Cycling Program appropriate for your Real-Time Instrument: Two-Step Cycling Program: 95ºC, 10 min; 40 cycles of (95ºC, 15 sec; and 60ºC, 60 sec); or  Extended Two-Step Cycling Program: 95ºC, 10 min; 45 cycles of (95ºC, 15 sec; and 60ºC, 60 sec); or Three-Step Cycling Program: 95ºC, 10 min; 40 cycles of (95ºC, 15 sec; 55ºC, 30-40 sec, and 72ºC, 30 sec) For the following products, please refer to the corresponding user manuals for the correct cycling programs: EpiTect Methyl PCR Arrays & DNA Methylation qPCR Assays RT² miRNA PCR Arrays & miRNA qPCR Assays EpiTect ChIP PCR Arrays & Real-time ChIP-PCR Primers Program the real-time thermal cycler to detect and record the SYBR® Green signal from every reaction at the end of the 60ºC annealing / extension step of each cycle. Run your instrument's default melting curve program immediately after the above PCR program, and generate a first derivative dissociation curve. No more than one peak should appear in each reaction If your instrument does not have a default melting curve program, run the following program instead: 95ºC, 1 min; 65ºC, 2 min (OPTICS OFF); 65ºC to 95ºC at 2ºC/ min (OPTICS ON). NOTE: Be sure to visually inspect the plate after the run for any signs of evaporation from any of the wells. If evaporation is observed, make a note of which wells so that you may qualify your data analysis appropriately. Back to Top

Performance

Protocol

Components

Please check the kit components immediately after you receive this package. We are not responsible for any missing items not reported within two (2) business days upon receipt. Contents: 330500, 330510, 330520: Two (2) tubes 330502, 330512, 330522: Twelve (12) tubes 330503, 330513, 330523: Twenty-four (24) tubes 330501, 330511, 330521: Eight (8) tubes Each tube contains 1.25 mL of 2X solution, sufficient for one PCR Array or for 100 25-µL reactions 330509, 330519, 330529: One (1) bottle Bottle contains 25 mL of 2X solution, sufficient for 2000 25-µL reactions NOTICE TO PURCHASER: LIMITED LICENSE Use of this product is covered by one of more of the following US patents and corresponding patent claims outside the US: 5,079,352, 5,789,224, 5,618,711, 6,127,155, 5,677,152 (claims 1 to 23 only), 5,773,258 (claims 1 and 6 only), 5,407,800, 5,322,770, 5,310,652, 5,994,056, 6,171,785, and claims outside the US corresponding to US Patent No. 4,889,818. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. Storage Conditions: The kit is shipped on dry ice or cold packs. For long-term storage, keep at -20 ºC. If entire volume is not be used all at once, divide into aliquots and store at -20 ºC. Avoid repeated freezing and thawing. Back to Top