Home > Products and Services > miRNA Home
miRNA expression profiling and quantification using the miScript PCR System
Mature microRNAs (miRNAs) are naturally occurring, 22-nucleotide, noncoding RNAs that mediate posttranscriptional gene regulation. miRNAs play an important role in many biological processes, including differentiation and development, cell signaling, and response to infection. Overwhelming evidence indicates that dysregulation of miRNA expression is a cause or indicator of several disease processes, including many cancers. The discovery that circulating miRNAs are detectable in serum and plasma, and that their expression varies as a result of disease, presents great potential for circulating miRNA expression signatures to be used as biomarkers in disease diagnosis and prevention. Success of miRNA research relies on access to high-performance tools and technologies. QIAGEN is the one-stop provider for all your miRNA purification, quantification, and functional characterization needs.
The miScript PCR System allows sensitive and specific detection and quantification of miRNA using SYBR Green real-time PCR. Regardless of whether you are interested in the expression of one miRNA or the entire miRNome, or whether you have only 1 ng of total RNA or 10 µg of total RNA, the miScript PCR System is the ideal miRNA expression quantification technology. Combined with industry-leading solutions for miRNA purification, the miScript PCR System delivers a sample-to-results solution for miRNA expression analysis. Each system component has been rigorously optimized to ensure unparalleled performance and results. With these technologies, you can accurately quantify miRNA expression in your cell line, tissue, FFPE, or body fluid of interest and be at the forefront of a rapidly growing, exciting scientific field.
The miScript PCR System offers:
- Straight-forward Workflows — Sample-to-results in less than one day, including free data analysis, regardless of sample type or the amount of sample available
- Leading miRNA Expression Profiling Solutions — miRNome and biologically relevant pathway-focused panels of mature miRNA primer assays
- Up-to-date Assay Coverage for Mature miRNAs — Individual primers assays for the most relevant miRNAs annotated in miRBase
- 100% Validated Primer Assays — All primers assays are designed, bench validated, and quality controlled to ensure sensitive and specific miRNA detection
The miScript PCR System comprises:
- miScript II RT Kit — this kit enables simple, single-step cDNA synthesis. A single cDNA synthesis reaction can be used for detection of hundreds of miRNAs. The dual buffer system meets the distinctive needs of miRNA quantification using real-time PCR. The unique, patent-pending formulation of miScript HiSpec Buffer facilitates the selective conversion of mature miRNAs into cDNA, which can then be used for miRNA quantification with either miScript miRNA PCR Arrays or miScript Primer Assays. Using miScript HiSpec Buffer, the conversion of long RNAs, such as mRNAs, is suppressed. As a result, background signals potentially contributed by long RNAs are nonexistent, making a reverse-transcription reaction buffered using miScript HiSpec Buffer an excellent choice for profiling the expression of mature miRNAs. miScript HiFlex Buffer is highly suited to low-through miRNA quantification experiments. Use of miScript HiFlex Buffer promotes conversion of all RNA species (mature miRNA, precursor miRNA, other noncoding RNA, and mRNA) into cDNA, and this cDNA can then be used to quantify each RNA species (using appropriate primer assays). 2’-O-methylation of is a hallmark of piRNAs and plant miRNAs, which prevents efficient and consistent addition of poly(A) homoribopolymer tails. As a result, the miScript II RT Kit is not recommended for reverse transcription of piRNAs and plant miRNAs.
- miScript PreAMP PCR Kit and Primer Mixes — this kit and primer mixes enable unbiased preamplification of limited miRNA amounts, allowing subsequent miRNA profiling with miScript miRNA PCR Arrays. This is particularly important when working with samples such as body fluids, formalin-fixed, paraffin-embedded (FFPE) samples, and small cell number samples such as laser capture microdissection (LCM) samples, flow-sorted cells, circulating tumor cells, and fine needle biopsies, where the low RNA yields obtained are often insufficient for reliable miRNA profiling experiments.
- miScript Primer Assay or miScript Precursor Assay — miScript Primer Assays are miRNA-specific forward primers designed to detect mature miRNAs. miScript Precursor Assays consist of forward and reverse primers targeting precursor miRNA stem-loop sequences. Assays are designed using the most up-to-date sequence information from miRBase.
- miScript SYBR Green PCR Kit — this kit includes QuantiTect SYBR Green PCR Master Mix and the miScript Universal Primer, a reverse primer that allows detection of miRNAs in combination with a miScript Primer Assay or miScript miRNA PCR Array.
- miScript miRNA PCR Array data analysis tool — this complimentary, Web-based tool simplifies the ΔΔCT method of relative quantification for miScript miRNA PCR Arrays.
Close
*Important: miScript PCR System components are NOT interchangeable with RT˛ miRNA PCR Array components or cDNAs prepared from RT˛ miRNA First Strand Kits. To finish established projects initiated with the RT˛ miRNA System, researchers may continue to reorder (for a limited time) RT˛ miRNA PCR Arrays, miRNA qPCR Assays and RT˛ miRNA First Strand Kits by contacting SABiosciences at 888-503-3187 or support@sabiosciences.com. To learn more about the RT˛ miRNA PCR Array Update, click here.
|
|
Complete Array List
