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miScript miRNA PCR Array Data Analysis
miScript miRNA PCR
Array Data Analysis Web Portal
This integrated web-based software package for miScript miRNA PCR Arrays is free to all customers and automatically performs all fold-change calculations from your uploaded CT values using the ΔΔCT method of relative quantification. Simply select the array's catalog number from a pull down menu to annotate the results with the correct gene list. The web portal delivers results not only in a tabular format but also in scatter, volcano, cluster-gram, and multi-group plots. Perform any pair-wise comparison between groups of experimental replicates defining your own fold-change and statistical significance thresholds, or compare all of the groups side-by-side. The web portal also helps you correctly interpret the reverse transcription efficiency and positive PCR control assays. Make your miRNA expression analysis as quick and painless as possible with the miScript miRNA PCR Arrays and the miScript miRNA PCR Array Data Analysis Web Portal.
Benefits of miScript miRNA PCR Array Data Analysis Tools:
Free of Charge — Complementary data analysis software available for each miScript miRNA PCR Array, including Custom miScript miRNA PCR Arrays
Ease of Use — Simply upload your data and find your catalog number. Results automatically analyzed in multiple formats and annotated to your gene list.
Convenience — Web-based application requires no downloading or installation. Stay current with latest gene content and software updates.
Security — Single session-based operation means that your data is not stored on any server. Export all processed data and results to an Excel file.
To obtain more detailed instructions on using this web portal, reserve
yourself a seat at one of our scheduled live web seminars, the "PCR Array
Data Analysis Tutorial".
Downloadable Excel-based miScript PCR Array Data Analysis Templates
If you prefer a non-web based analysis alternative, download the appropriate
Excel file below. Be sure to save it to your local computer before opening the
file and entering miRNA PCR Array data for analysis.
| 1. |
96-well plate, miScript miRNA PCR Arrays |
96 |
| |
For 384-well plates using the 4 x 96 array
format, convert your 384-well raw data into four sets of 96-well data with
this Patch.
Then cut-and-paste into the appropriate worksheet of the Data Analysis
Template (above). |
| 2. |
miRNome miScript miRNA PCR Arrays (based on #
of plates to cover the miRNome) |
|
|
- 96-well plate format Arrays
|
|
- 12 Plates per miRNome Arrays
|
MIXX-2##_12Plate |
- 8 Plates per miRNome Arrays
|
MIXX-2##_8Plate |
- 4 Plates per miRNome Arrays
|
MIXX-2##_4Plate |
- 384-well plate format Arrays
|
|
-
3 Plates per miRNome Arrays
|
MIXX-32##_3Plate |
-
2 Plates per miRNome Arrays
|
MIXX-32##_2Plate |
-
1 Plate per miRNome Arrays
|
MIXX-32##_1Plate |
| 3. |
miScript miRNA QC PCR Arrays
|
96 |
Note: If you are using RT² miRNA PCR Arrays please use the link below
for the Data Analysis Web Portal or select a template from the table below.
RT² PCR Array Data
Analysis Web Portal
Downloadable Excel-based PCR Array Data Analysis Templates
If you prefer, download the appropriate Excel file below. Be sure to save it
to your local computer before opening it and entering miRNA PCR Array data for
analysis.
If you are using:
| 1. |
96-well Pathway-focused miRNA PCR Arrays |
96PM |
| |
For 384-well plates using the 4 x 96 array
format, convert your 384-well raw data into four sets of 96-well data with
this Patch.
Then cut-and-paste into the appropriate worksheet of the Data Analysis
Template (above). |
| 2. |
Genome V2.0 miRNA PCR Arrays (miRBase 14) |
|
|
|
|
|
|
MAH-100 |
MAH-200 |
| MAH-100&200 |
|
|
MAM-100 |
MAM-200 |
|
|
MAR-100 |
- 384-well miRNA PCR Arrays
|
|
-
Human: MAH-3200 (MAH-3100 & MAH-3101)
|
384
Human/Mouse |
-
Mouse: MAM-3200 (MAM-3100 & MAM-3101)
|
|
|
384
Rat miRNome |
| 3. |
384-well plates in the 4 x 96 format
|
384-4x96mi |
|
For 384-well plates in the 4 x 96 format, convert your 384-well raw data into
four sets of 96-well data with this Patch.
Then cut-and-paste into the correct worksheet and cells do the miRNA PCR
Array Data Analysis Template (above).
|
|