Genome-Wide RT² miRNA qPCR Assays
The RT² miRNA PCR Array System was retired on October 1, 2011 and replaced
with the miScript miRNA PCR Array System.
Important: miScript miRNA PCR System components are NOT interchangeable with
RT² miRNA PCR Array components or cDNAs prepared from RT² miRNA First Strand
To finish established projects initiated with the RT² miRNA System,
researchers may continue to reorder for a limited time RT² miRNA PCR Arrays, RT²
miRNA qPCR Assays and RT² miRNA First Strand Kits by contacting SABiosciences at
888-503-3187 or firstname.lastname@example.org.
To continue to the new miScript Primer Assay web page on QIAGEN GeneGlobe for
the latest assays designed to miRBase version 16, please click here.
The RT² miRNA qPCR Assays are the most reliable and accurate SYBR Green-based
quantitative real-time PCR assay for analyzing the expression of any miRNA
sequence. Our experimentally verified primer design algorithm distinguishes
miRNA family members with single nucleotide mismatches. The resulting
miRNA-specific qPCR assays are experimentally certified for their specificity
and uniformly high amplification efficiencies under standardized cycling
conditions. The high performance of every RT² miRNA qPCR Assay is 100% guaranteed
when performed using the RT² miRNA First Strand Kit and RT² SYBR Green qPCR Master
Search for Your miRNA of Interest:
Why miRNA PCR Assays?
- High performance: Each
assay is experimentally validated to amplify a single amplicon with
uniform PCR efficiency. The performance of these SYBR Green assays
for miRNA expression analysis is similar to that of TaqMan®, miScript,
NCode™, miRCURY LNA™, and mirVana™ Assays.
- Complete Genome Coverage:
Assays are available for every over 400 majority miRNA sequences.
The uniform PCR efficiency and PCR conditions of the assays allow
for an easy, scalable transition from single gene analysis to
multiple gene expression analyses.
- Simple & Convenient:
The universal reverse transcription reaction converts all miRNA
sequences into cDNA in a one-step reaction. Assays deliver
guaranteed performance when used with SABiosciences' optimized
instrument-specific qPCR master mixes for ABI, Bio-Rad, Stratagene
and other real-time PCR instruments.
HOW IT WORKS:
A miRNA-specific primer and a universal PCR primer amplify miRNA cDNA
during real-time PCR for SYBR Green-based detection. The universal PCR
priming sequence incorporated during reverse transcription and the
uniformly high amplification efficiencies provide a scalable solution
for analyzing the expression of multiple miRNA sequences in the same
cDNA preparation under standardized conditions. The nature of the
universal priming sequence requires the use of the RT² miRNA First Strand Kit.
Specificity: Discrimination of Single Nucleotide Mismatches
Our proprietary primer design and reaction formulation distinguishes miRNA
family members with single nucleotide mismatches, providing greater
discrimination or specificity than other assays.
Sensitivity & Dynamic Range
Patent-pending chemistry preferentially reverse transcribes mature miRNA,
thereby decreasing non-specific background and increasing sensitivity over other
assays. The wide dynamic range typical for real-time PCR allows miRNA
sequences at a wide variety of expression levels to be detected simultaneously.
The high degree of technical reproducibility demonstrated by the miRNA PCR
Arrays means that results can be reliably compared across cycling runs, array
plates, and samples.
You can easily perform a PCR Array experiment in your own laboratory,
or send your samples to us and take advantage of our RT²
qPCR Gene Expression Analysis Services.
Necessary qPCR Reagents for Guaranteed Performance:
RT² qPCR-Grade miRNA Isolation Kit
To enrich RNA samples for miRNA
RT² miRNA First Strand Kit
For cDNA sequences compatible with universal assay
RT² SYBR Green qPCR Master Mix
Special formulations optimized for your real-time PCR
Housekeeping qPCR Assays
For small nuclear RNA assays and data normalization