QIAGEN Website    Quick Order    Online Seminar    Contact    My Account
Home  >  Products  >  miRNA Home  >  miRNA PCR Array  >   Prostate Cancer

Prostate Cancer miRNA PCR Array

Human Mouse Rat Other Species
 
Human Prostate Cancer miRNA PCR Array: MIHS-112Z
The Human Prostate Cancer miScript miRNA PCR Array profiles the expression of 84 miRNAs known to alter their expression during prostate cancer progression and development. This array provides cancer researchers with a convenient way to quickly analyze the miRNAs most relevant to prostate cancer. As an adenocarcinoma or glandular cancer, prostate cancer originates from prostate gland cells. Most prostate cancers grow slowly and respond to androgen ablation therapy (androgen-dependent or AD). However, such tumors eventually become refractory to this therapy, and progress into androgen-independent (castration-resistant) prostate cancers. Perineural invasion, where cancer cells adhere to and wrap around nerves, is the predominant method for prostate cancer local invasion, extraprostatic spread, and metastasis. The molecular mechanisms behind these processes are not well understood, but the discovery of aberrantly regulated miRNA in biopsies from various prostate cancers and in circulation suggests a correlation with the progress of carcinogenesis. This array contains differentially regulated miRNAs based on microarray, real-time RT-PCR, and deep sequencing expression profiling studies involving a broad spectrum of prostate cancer clinical samples and cell lines. The profiling results from this array may yield insights into the molecular mechanisms behind the pathogenesis of prostate cancer. A set of controls present on this array enables data analysis using the ΔΔCT method of relative quantification, assessment of reverse transcription performance, and assessment of PCR performance. Using SYBR Green-based real-time PCR, the expression of a focused panel of miRNAs related to prostate cancer can be easily and reliably analyzed with this miScript miRNA PCR Array.

miScript miRNA PCR Arrays are intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

96-well, 384-well ( 4 x 96 ), and Rotor-Disc 100 formats are available.
See the complete miRNA PCR Array list

 

Functional miRNA Grouping How It Works Manual & Resources Reagents & Software
 
Pathway Source References     Gene Table

Prostate Cancer Versus Normal Tissue or Benign Prostatic Hyperplasia:
Up-regulated: let-7b-5p, let-7f-5p, miR-106b-5p, miR-141-3p, miR-148a-3p, miR-15b-5p, miR-181a-5p, miR-182-5p, miR-183-5p, miR-184, miR-194-5p, miR-196a-5p, miR-200c-3p, miR-20a-5p, miR-21-5p, miR-218-5p, miR-25-3p, miR-26a-5p, miR-26b-5p, miR-32-5p, miR-375, miR-425-5p, miR-96-5p.
Down-regulated: let-7a-5p, miR-100-5p, miR-101-3p, miR-125b-5p, miR-126-3p, miR-126-5p, miR-128, miR-133a, miR-143-3p, miR-145-5p, miR-17-3p, miR-181b-5p, miR-203a, miR-205-5p, miR-223-3p, miR-23b-3p, miR-27a-3p, miR-27b-3p, miR-296-5p, miR-29b-3p, miR-31-5p, miR-330-3p, miR-34a-5p, miR-34b-3p, miR-34c-5p, miR-449a, miR-494, miR-7-5p, miR-93-5p.
Regulated: let-7c, miR-125a-5p, miR-16-5p, miR-17-5p//miR-106a-5p, miR-22-3p, miR-221-3p, miR-222-3p, miR-24-3p, miR-92a-3p, miR-99b-5p.

Androgen-Independent (Castration Resistant Prostate Cancer) Versus Dependent Cancers:
Up-regulated: miR-135b-5p, miR-15a-5p, miR-184, miR-21-5p, miR-221-3p, miR-30c-5p, miR-361-5p, miR-455-5p, miR-616-3p.
Down Regulated: miR-100-5p, miR-125b-5p, miR-128, miR-146a-5p, miR-146b-5p, miR-19b-3p, miR-203a, miR-23b-3p, miR-27b-3p, miR-29b-3p, miR-331-3p, miR-365a-3p, miR-99a-5p.
Regulated: let-7c, miR-135a-5p, miR-16-5p, miR-17-5p//miR-106a-5p, miR-222-3p, miR-92a-3p, miR-99b-5p.

Androgen Receptor-Regulated miRNAs:
Up-regulated: miR-148a-3p, miR-15b-5p, miR-20a-5p, miR-20b-5p, miR-21-5p.
Down-regulated: miR-19b-3p, miR-29b-3p, miR-93-5p.
Regulated: miR-16-5p, miR-17-5p//miR-106a-5p

Perineural Invasion:
Up-regulated: miR-15a-5p, miR-181a-5p, miR-195-5p, miR-21-5p, miR-224-5p, miR-26a-5p, miR-30c-5p.
Down regulated: miR-100-5p, miR-125b-5p, miR-126-3p, miR-145-5p, miR-27a-3p, miR-27b-3p.
Regulated: miR-24-3p, miR-99b-5p.

Aggressive Versus Non-aggressive Prostate Cancer:
Up-regulated: miR-9-3p.
Down-regulated: miR-145-5p, miR-221-3p, miR-330-3p, miR-331-3p, miR-34c-5p.
Regulated: miR-16-5p.

Blood, Plasma, and Serum Markers:
Up-regulated: miR-141-3p, miR-200b-3p, miR-21-5p, miR-375, miR-9-3p.
Regulated: miR-221-3p, miR-26a-5p.

miRNAs Predicted to Regulate Prostate Cancer Genes: miR-3163, miR-3662, miR-3666, miR-374b-5p.

Back to Top
 

Functional miRNA Grouping How It Works Manual & Resources Reagents & Software
 

What miScript miRNA PCR Arrays offer?
   Guaranteed Performance* - ready-to-use for gene expression analysis
   Time & Cost Saving - less than 30 min hands-on time to analyze 84 or 372 miRNA
   Simple Data Analysis - easy-to-use Excel-Based Data Analysis Template

Layout and Controls: miScript miRNA PCR Arrays are available in 96-well, 384-well, and Rotor-Disc 100 formats. Included on each array is a set of six normalization controls as well as controls to assess RNA recovery, reverse transcription performance, and PCR performance.

You can easily perform a PCR Array experiment in your own laboratory, or send your samples to us and take advantage of our PCR Array Gene Expression Analysis Services.

*: when using the Complete miRNA PCR Array System.

Back to Top

 

Functional miRNA Grouping How It Works Manual & Resources Reagents & Software
 

User Manual

miScript miRNA PCR Array Handbook  (PDF)

White Paper

miRNA Profiling From Blood Challenges and Recommendations (PDF)

Data Analysis

Free miRNA PCR Array Data Analysis Software (Web & Excel based)

Required Reagents and Setup Instructions

Materials & Equipment Required for miScript miRNA PCR Products
miRNA PCR Array Instrument Setup Instructions & Protocol Files

Web Seminars

Attend a live on-line seminar hosted by an Applications Scientist about PCR

Technical Brochures

miScript PCR System Brochure (PDF)

Pathway Central

Pathway reviews and presentation-ready pathway maps

Back to Top
 

Functional miRNA Grouping How It Works Manual & Resources Reagents & Software
 

The complete miScript miRNA PCR Array System Includes:

Data Analysis: miRNA PCR Array Data Analysis Software
Back to Top