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Hypoxia Signaling Pathway miScript miRNAs PCR Array

Human Mouse Rat Other Species
Human Hypoxia Signaling Pathway miScript miRNAs PCR Array: MIHS-121Z
The Human Hypoxia Signaling Pathway miScript miRNA PCR Array profiles the expression of 84 miRNAs relevant to cellular responses to hypoxic conditions. Mammalian cells can be exposed to hypoxic conditions during development, in stem cell niches, or in pathological states such as in solid cancer tumors or during ischemia due to physical trauma, heart attack or stroke. Cells respond to low oxygen concentrations by adjusting gene expression. The primary sensor and regulator of hypoxic responses is the hypoxia-inducible factor α (HIFα). In the presence of normal oxygen levels, HIFα protein is rapidly hydroxylated which targets it for ubiquitination and degradation by the von Hippel Lindau protein (VHL). Under hypoxic conditions, HIFα is stable and is able to heterodimerize with its transcriptional co-activator ARNT (HIFβ), translocate to the nucleus, and alter gene expression. In addition to changes in mRNA expression, the HIFα/ARNT complex induces changes in miRNA expression that create feedback loops to regulate the expression of genes in the HIFα pathway and that also regulate genes involved in cellular responses to hypoxia. This array contains assays that target miRNA whose roles in the hypoxia signaling pathway have been experimentally demonstrated. The miRNA represented by the array increase or decrease under hypoxic conditions, regulate genes in the HIFα pathway, or regulate genes important in cellular responses to hypoxia. Differences in the expression levels of these miRNA detected by this array may yield insights into the molecular mechanisms underlying differential responses to hypoxia and may provide useful molecular markers for cells that are responding to hypoxic conditions. A set of controls present on each array enables data analysis using the ΔΔCT method of relative quantification, assessment of reverse transcription performance, and assessment of PCR performance. Using SYBR Green real-time PCR, the expression of a focused panel of miRNAs related to hypoxia signaling can be easily and reliably analyzed with this miScript miRNA PCR Array.

96-well, 384-well ( 4 x 96 ), and Rotor-Disc 100 formats are available.
See the complete miRNA PCR Array list


Functional miRNA Grouping How It Works Manual & Resources Reagents & Software
Pathway Source References     Gene Table

Regulators of HIF Signaling Pathway:
Regulators of HIF1A: miR-107, miR-130a-3p, miR-130b-3p, miR-138-5p, miR-155-5p, miR-17-5p, miR-199a-5p, miR-199b-5p, mir-20a-5p, mir-20b-5p, miR-92a-3p, miR-935.
Regulators of ARNT: miR-107, miR-215-5p, miR-324-5p, miR-877-3p.
Regulators of HIF1A Inhibitors: miR-24-3p, miR-93-5p.

Regulated by Hypoxia:
Down-Regulated by Hypoxia: let-7a-5p, let-7c-5p, let-7d-5p, let-7f-5p, miR-101-3p, miR-122-5p, miR-135a-5p, miR-141-3p, miR-15b-5p, miR-16-5p, miR-186-5p, miR-19a-3p, miR-199a-5p, miR-200b-3p, miR-22-3p, miR-224-5p, miR-29b-3p, miR-30e-5p, miR-320a, miR-331-3p, miR-378a-3p, miR-449a, miR-92a-3p.
Up-Regulated by Hypoxia: let-7b-5p, let-7e-5p, let-7g-5p, let-7i-5p, miR-10b-5p, miR-103a-3p, miR-107, miR-125a-5p, miR-125b-5p, miR-130a-3p, miR-130b-3p, miR-146a-5p, miR-146b-5p, miR-148a-3p, miR-148b-3p, miR-15a-5p, miR-150-5p, miR-155-5p, miR-181a-5p, miR-181b-5p, miR-181c-5p, miR-188-5p, miR-191-5p, miR-192-5p, miR-195-5p, miR-200a-3p, miR-204-5p, miR-205-5p, miR-21-5p, miR-210-3p, miR-221-3p, miR-23a-3p, miR-23b-3p, miR-24-3p, miR-26a-5p, miR-26b-5p, miR-27a-3p, miR-30b-5p, miR-31-5p, miR-335-5p, miR-429, miR-451a, miR-491-5p, miR-504-5p, miR-7-5p, miR-9-5p, miR-93-5p, miR-98-5p.

Regulators of Hypoxia Responsive Genes: let-7b-5p, miR-10b-5p, miR-103a-3p, miR-125a-5p, miR-125b-5p, miR-146a-5p, miR-146b-5p, miR-15b-5p, miR-150-5p, miR-155-5p, miR-16-5p, miR-181c-5p, miR-184, miR-20a-5p, miR-20b-5p, miR-203a, miR-21-5p, miR-210-3p, miR-221-3p, miR-23b-3p, miR-26a-5p, miR-324-5p, miR-331-3p, miR-34a-5p, miR-378a-3p, miR-504-5p, miR-9-5p, miR-93-5p, miR-99a-5p.

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Functional miRNA Grouping How It Works Manual & Resources Reagents & Software

What miScript miRNA PCR Arrays offer?
   Guaranteed Performance* - ready-to-use for gene expression analysis
   Time & Cost Saving - less than 30 min hands-on time to analyze 84 or 372 miRNA
   Simple Data Analysis - easy-to-use Excel-Based Data Analysis Template

Layout and Controls: miScript miRNA PCR Arrays are available in 96-well, 384-well, and Rotor-Disc 100 formats. Included on each array is a set of six normalization controls as well as controls to assess RNA recovery, reverse transcription performance, and PCR performance.

You can easily perform a PCR Array experiment in your own laboratory, or send your samples to us and take advantage of our PCR Array Gene Expression Analysis Services.

*: when using the Complete miRNA PCR Array System.

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Functional miRNA Grouping How It Works Manual & Resources Reagents & Software

User Manual

miScript miRNA PCR Array Handbook  (PDF)

White Paper

miRNA Profiling From Blood – Challenges and Recommendations (PDF)

Data Analysis

Free miRNA PCR Array Data Analysis Software (Web & Excel based)

Required Reagents and Setup Instructions

Materials & Equipment Required for miScript miRNA PCR Products
miRNA PCR Array Instrument Setup Instructions & Protocol Files

Web Seminars

Attend a live on-line seminar hosted by an Applications Scientist about PCR

Technical Brochures

miScript PCR System Brochure (PDF)

Pathway Central

Pathway reviews and presentation-ready pathway maps

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Functional miRNA Grouping How It Works Manual & Resources Reagents & Software

The complete miScript miRNA PCR Array System Includes:

Data Analysis: miRNA PCR Array Data Analysis Software
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