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Serum & Plasma 384HC

Human Mouse
Human Serum & Plasma 384HC miRNA PCR Array: MIHS-3106Z
The Human Serum & Plasma 384HC miScript miRNA PCR Array profiles the expression of 372 miRNAs detectable in serum and plasma using the miScript PCR System. This high-content array also includes the 84 miRNAs detectable and differentially expressed in serum, plasma, and other bodily fluids that are found on the Human Serum & Plasma miScript miRNA PCR Array. The serum and plasma 384HC array provides disease and toxicology researchers with a convenient way to quickly analyze the extracellular, circulating miRNAs most relevant to pathophysiological conditions. The discovery of miRNAs in serum and plasma, and their apparently regulated levels there, suggest a mechanistic role for these master regulatory molecules in both normal and pathophysiological processes. Their presence in serum and plasma samples provides readily available source material for research purposes. The profiling results from this array can potentially serve as a useful molecular marker for heart and liver injury or disease, atherosclerosis, diabetes, and a number of organ-specific cancers. This array can also be used with miRNeasy Serum/Plasma Spike-In Control (catalog number 219610). When spiked into the sample before nucleic acid isolation and detected with the control assay, it provides a more useful normalization method for this application than the typical reference short noncoding RNAs, which are not normally detected in serum. A set of controls present on this array enables data analysis using the ΔΔCT method of relative quantification, assessment of reverse transcription performance, and assessment of PCR performance. Using SYBR Green-based real-time PCR, the expression of a focused panel of miRNAs found in serum and plasma can be easily and reliably analyzed with this miScript miRNA PCR Array.

miScript miRNA PCR Arrays are intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Available in 384-well format.
See the complete miRNA PCR Array list

miRNA White Paper

Gene Table


How It Works Manual & Resources Reagents & Software

What miScript miRNA PCR Arrays offer?
   Guaranteed Performance* - ready-to-use for gene expression analysis
   Time & Cost Saving - less than 30 min hands-on time to analyze 84 or 372 miRNA
   Simple Data Analysis - easy-to-use Excel-Based Data Analysis Template

Layout and Controls: miScript miRNA PCR Arrays are available in 96-well, 384-well, and Rotor-Disc 100 formats. Included on each array is a set of six normalization controls as well as controls to assess RNA recovery, reverse transcription performance, and PCR performance.

You can easily perform a PCR Array experiment in your own laboratory, or send your samples to us and take advantage of our PCR Array Gene Expression Analysis Services.

*: when using the Complete miRNA PCR Array System.

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How It Works Manual & Resources Reagents & Software

User Manual

miScript miRNA PCR Array Handbook  (PDF)

White Paper

miRNA Profiling From Blood Challenges and Recommendations (PDF)

Data Analysis

Free miRNA PCR Array Data Analysis Software (Web & Excel based)

Required Reagents and Setup Instructions

Materials & Equipment Required for miScript miRNA PCR Products
miRNA PCR Array Instrument Setup Instructions & Protocol Files

Web Seminars

Attend a live on-line seminar hosted by an Applications Scientist about PCR

Technical Brochures

miScript PCR System Brochure (PDF)

Pathway Central

Pathway reviews and presentation-ready pathway maps

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How It Works Manual & Resources Reagents & Software

The complete miScript miRNA PCR Array System Includes:

Data Analysis: miRNA PCR Array Data Analysis Software
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