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Serum & Plasma miRNA PCR Array

Human Mouse Rat
 
Mouse Serum & Plasma miRNA PCR Array: MIMM-106Z
The Mouse Serum & Plasma miScript miRNA PCR Array profiles the expression of 84 miRNAs detectable and differentially expressed in serum, plasma, and other bodily fluids. This array provides disease and toxicology researchers with a convenient way to quickly analyze the miRNAs most relevant to pathophysiological conditions. These miRNAs have been carefully selected based on results published in peer-reviewed journals that suggest a correlation with serum expression levels and specific diseases. The discovery of these master regulatory molecules in serum and biological fluids, and their apparently regulated levels there, suggest a mechanistic role for miRNA in both normal and pathophysiological processes. Their presence in noninvasive, easily accessible samples provides readily available source material for research purposes. The profiling results from this array can potentially serve as a useful molecular marker for heart and liver injury or disease, atherosclerosis, diabetes, and a number of organ-specific cancers. Other miRNAs routinely present in serum are included to serve as positive controls for the successful detection of serum miRNA expression. This array can also be used with miRNeasy Serum/Plasma Spike-In Control (catalog number 219610). When spiked into the sample before nucleic acid isolation and detected with the control assay, it provides a more useful normalization method for this application than the typical reference short noncoding RNAs not normally detected in serum. A set of controls present on this array enables data analysis using the ΔΔCT method of relative quantification, assessment of reverse transcription performance, and assessment of PCR performance. Using SYBR Green-based real-time PCR, the expression of a focused panel of miRNAs found in serum, plasma, and other bodily fluids can be easily and reliably analyzed with this miScript miRNA PCR Array.

miScript miRNA PCR Arrays are intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

96-well, 384-well ( 4 x 96 ), and Rotor-Disc 100 formats are available.
See the complete miRNA PCR Array list


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Functional miRNA Grouping How It Works Manual & Resources Reagents & Software
 
Pathway Source References     Gene Table

Injury:
Heart Injury: miR-1a-3p, miR-133a-3p, miR-423-5p, miR-499-5p.
Liver Injury: miR-122-5p, miR-192-5p.

Disease:
Atherosclerosis: miR-150-5p.
Diabetes: miR-124-3p, miR-146a-5p, miR-29a-3p, miR-30d-5p, miR-34a-5p, miR-375-3p, miR-9-5p.
Heart Disease: miR-133a-3p.
Liver Disease: miR-146a-5p, miR-215-5p, miR-224-5p, miR-574-3p, miR-92a-3p.

Cancer:
Adenocarcinoma: miR-29a-3p, miR-92a-3p.
Breast: let-7a-5p, miR-106a-5p, miR-10b-5p, miR-141-3p, miR-155-5p, miR-195a-5p, miR-21a-5p, miR-34a-5p.
Colon: miR-134-5p, miR-146a-5p, miR-17-3p, miR-221-3p, miR-222-3p, miR-23a-3p, miR-29a-3p, miR-92a-3p.
Gastric: let-7a-5p, miR-1a-3p, miR-106a-5p, miR-106b-5p, miR-17-5p, miR-17-3p, miR-20a-5p, miR-21a-5p, miR-27a-3p, miR-34a-5p, miR-423-5p.
Leukemia & B Cell Lymphoma: miR-155-5p, miR-21a-5p, miR-210-3p.
Liver: miR-122-5p, miR-199a-5p, miR-21a-5p, miR-223-3p.
Lung: miR-134-5p, miR-146a-5p, miR-17-3p, miR-21a-5p, miR-210-3p, miR-221-3p, miR-222-3p, miR-223-3p, miR-23a-3p, miR-25-3p.
Ovarian: miR-126-3p, miR-141-3p, miR-200a-3p, miR-200b-3p, miR-200c-3p, miR-203-3p, miR-205-5p, miR-21a-5p, miR-214-3p, miR-29a-3p, miR-92a-3p, miR-93-5p.
Pancreatic: miR-196a-5p, miR-200a-3p, miR-200b-3p, miR-21a-5p, miR-210-3p.
Prostate: miR-100-5p, miR-125b-5p, miR-141-3p, miR-143-3p, miR-18a-5p, miR-19a-3p, miR-19b-3p, miR-20a-5p, miR-21a-5p, miR-296-5p, miR-375-3p.
Renal: miR-124-3p.
Rhabdomyosarcoma: miR-1a-3p, miR-133a-3p, miR-133b-3p.

Other Cancer-Related miRNA: let-7c-5p, miR-107-3p, miR-10a-5p, miR-128-3p, miR-130b-3p, miR-145a-5p, miR-148a-3p, miR-15a-5p, miR-181a-5p, miR-181b-5p, miR-184-3p, miR-193a-3p, miR-204-5p, miR-206-3p, miR-211-5p, miR-26b-5p, miR-30e-5p, miR-31-5p, miR-34c-5p, miR-376c-3p, miR-7a-5p, miR-96-5p.

Routinely Detectable in Normal Serum: miR-103-3p, miR-15b-5p, miR-16-5p, miR-191-5p, miR-22-3p, miR-24-3p, miR-26a-5p.

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Functional miRNA Grouping How It Works Manual & Resources Reagents & Software
 

What miScript miRNA PCR Arrays offer?
   Guaranteed Performance* - ready-to-use for gene expression analysis
   Time & Cost Saving - less than 30 min hands-on time to analyze 84 or 372 miRNA
   Simple Data Analysis - easy-to-use Excel-Based Data Analysis Template

Layout and Controls: miScript miRNA PCR Arrays are available in 96-well, 384-well, and Rotor-Disc 100 formats. Included on each array is a set of six normalization controls as well as controls to assess RNA recovery, reverse transcription performance, and PCR performance.

You can easily perform a PCR Array experiment in your own laboratory, or send your samples to us and take advantage of our PCR Array Gene Expression Analysis Services.

*: when using the Complete miRNA PCR Array System.

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Functional miRNA Grouping How It Works Manual & Resources Reagents & Software
 

User Manual

miScript miRNA PCR Array Handbook  (PDF)

White Paper

miRNA Profiling From Blood Challenges and Recommendations (PDF)

Data Analysis

Free miRNA PCR Array Data Analysis Software (Web & Excel based)

Required Reagents and Setup Instructions

Materials & Equipment Required for miScript miRNA PCR Products
miRNA PCR Array Instrument Setup Instructions & Protocol Files

Web Seminars

Attend a live on-line seminar hosted by an Applications Scientist about PCR

Technical Brochures

miScript PCR System Brochure (PDF)

Pathway Central

Pathway reviews and presentation-ready pathway maps

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Functional miRNA Grouping How It Works Manual & Resources Reagents & Software
 

The complete miScript miRNA PCR Array System Includes:

Data Analysis: miRNA PCR Array Data Analysis Software
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