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Fibrosis miRNA PCR Array

Human Mouse Rat Other Species
 
Mouse Fibrosis miRNA PCR Array: MIMM-117Z
The Mouse Fibrosis miScript miRNA PCR Array profiles the expression of 84 miRNAs known to play a role in fibrosis. During tissue remodeling that occurs as part of wound healing, apoptosis removes myofibroblasts, and the deposited extracellular matrix (ECM) is remodeled to resemble the original tissue. Fibrosis occurs when inappropriate tissue remodeling results in excess ECM deposition due to inappropriate survival of myofibroblasts or lack of ECM proteolytic degradation. The inflammation and enhanced TGFβ signaling often present in fibrotic tissues causes cells to differentiate into myofibroblasts via epithelial-to-mesenchymal transition (EMT). miRNAs may play a role in conducting the pathogenesis of fibrosis by modulating the expression of genes involved in ECM remodeling and cell adhesion, inflammation, angiogenesis, TGFβ/CTGF signaling, and EMT. This array includes miRNAs that are differentially expressed during fibrosis and are known or predicted to modulate the expression of pro- and anti-fibrotic genes common to different fibrotic disorders such as systemic sclerosis, as well as liver, cardiac, kidney, and idiopathic pulmonary fibrosis. The profiling results from this array may yield insights into the molecular mechanisms behind the pathogenesis of fibrosis. A set of controls present on each array enables data analysis using the ΔΔCT method of relative quantification, assessment of reverse transcription performance, and assessment of PCR performance. Using SYBR Green real-time PCR, the expression of a focused panel of miRNAs involved in fibrosis can be easily and reliably analyzed with this miScript miRNA PCR Array.

miScript miRNA PCR Arrays are intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

96-well, 384-well ( 4 x 96 ), and Rotor-Disc 100 formats are available.
See the complete miRNA PCR Array list

 

Functional miRNA Grouping How It Works Manual & Resources Reagents & Software
 
Pathway Source References     Gene Table

Pro-Fibrotic: miR-142-3p, miR-145a-5p, miR-138-5p, miR-205-5p, miR-21a-5p, miR-216a-5p, miR-27a-3p, miR-27b-3p, miR-32-5p, miR-338-5p, miR-34a-5p, miR-377-3p, miR-382-5p, miR-99a-5p, miR-192-5p, miR-30a-5p, miR-208a-3p, miR-147-3p.

Anti-Fibrotic: let-7d-5p, miR-107-3p, miR-132-3p, miR-133a-3p, miR-141-3p, miR-15b-5p, miR-16-5p, miR-17-5p, miR-18a-5p, miR-194-5p, miR-19a-3p, miR-19b-3p, miR-200a-3p, miR-200b-3p, miR-204-5p, miR-20a-5p, miR-26a-5p, miR-26b-5p, miR-29b-3p, miR-335-5p, miR-449a-5p, miR-590-5p.

Extracellular Matrix Remodeling & Cell Adhesion: miR-199a-5p, miR-29a-3p, miR-29b-3p, miR-29c-3p, miR-449a-5p, miR-21a-5p, miR-204-5p, miR-25-3p, miR-7a-5p, miR-196a-5p, miR-203-3p, miR-27b-3p, miR-27a-3p, miR-451a, miR-145a-5p, miR-143-3p, miR-1a-3p, miR-10a-5p, miR-10b-5p, miR-30a-5p, miR-16-5p, miR-365-3p, miR-669d-3p, miR-1961.

Inflammation: let-7d-5p, miR-204-5p, miR-122-5p, miR-146a-5p, miR-199a-5p, miR-466l-3p, miR-21a-5p, miR-129-5p, miR-142-3p, miR-32-5p, miR-181b-5p.

Angiogenesis: let-7d-5p, miR-378a-3p, miR-322-5p, miR-34a-5p, miR-29b-3p, miR-20a-5p, miR-200b-3p, miR-195a-5p, miR-17-5p, miR-16-5p, miR-15b-5p, miR-150-5p, miR-145a-5p, miR-126-3p, miR-107-3p, miR-338-5p, miR-375-3p, miR-467a-3p.

Signal Transduction & Transcriptional Regulation: miR-26a-5p, miR-18a-5p, miR-122-5p, miR-133a-3p, miR-20a-5p, miR-203-3p, miR-19a-3p, miR-17-5p, miR-146a-5p, miR-377-3p, miR-491-5p, miR-29b-3p, miR-141-3p, miR-29a-3p, miR-204-5p, miR-590-5p, miR-138-5p, miR-302b-3p, miR-19b-3p, miR-21a-5p, miR-1a-3p, miR-23a-3p, miR-328-3p, miR-744-5p, miR-148a-3p, miR-451a, miR-125b-5p, miR-449a-5p, miR-29c-3p, miR-195a-5p, miR-192-5p, miR-16-5p, miR-15b-5p, miR-99a-5p, miR-30a-5p, miR-378a-3p, miR-34a-5p, miR-145a-5p, miR-146b-5p, miR-27a-3p, miR-324-5p, miR-467a-3p, miR-223-3p, miR-101a-3p, miR-216a-5p, miR-3094-5p.

Epithelial-to-Mesenchymal Transition: let-7d-5p, miR-107-3p, miR-297b-3p, miR-199a-5p, miR-330-3p, miR-200a-3p, miR-200b-3p, miR-128-3p, miR-29a-3p, miR-29b-3p, miR-29c-3p, miR-382-5p, miR-136-5p, miR-874-3p.

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Functional miRNA Grouping How It Works Manual & Resources Reagents & Software
 

What miScript miRNA PCR Arrays offer?
   Guaranteed Performance* - ready-to-use for gene expression analysis
   Time & Cost Saving - less than 30 min hands-on time to analyze 84 or 372 miRNA
   Simple Data Analysis - easy-to-use Excel-Based Data Analysis Template

Layout and Controls: miScript miRNA PCR Arrays are available in 96-well, 384-well, and Rotor-Disc 100 formats. Included on each array is a set of six normalization controls as well as controls to assess RNA recovery, reverse transcription performance, and PCR performance.

You can easily perform a PCR Array experiment in your own laboratory, or send your samples to us and take advantage of our PCR Array Gene Expression Analysis Services.

*: when using the Complete miRNA PCR Array System.

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Functional miRNA Grouping How It Works Manual & Resources Reagents & Software
 

User Manual

miScript miRNA PCR Array Handbook  (PDF)

White Paper

miRNA Profiling From Blood Challenges and Recommendations (PDF)

Data Analysis

Free miRNA PCR Array Data Analysis Software (Web & Excel based)

Required Reagents and Setup Instructions

Materials & Equipment Required for miScript miRNA PCR Products
miRNA PCR Array Instrument Setup Instructions & Protocol Files

Web Seminars

Attend a live on-line seminar hosted by an Applications Scientist about PCR

Technical Brochures

miScript PCR System Brochure (PDF)

Pathway Central

Pathway reviews and presentation-ready pathway maps

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Functional miRNA Grouping How It Works Manual & Resources Reagents & Software
 

The complete miScript miRNA PCR Array System Includes:

Data Analysis: miRNA PCR Array Data Analysis Software
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