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T-Cell & B-Cell Activation miRNA PCR Array

Human Mouse Rat Other Species
 
Rat T-Cell & B-Cell Activation miRNA PCR Array: MIRN-111Z
The Rat T-Cell & B-Cell Activation miScript miRNA PCR Array profiles the expression of 84 miRNAs involved in the activation and differentiation of T cells and B cells. This array provides scientific researchers with a convenient way to quickly analyze the miRNAs most relevant to specific stages of T cell and B cell development. Hematopoietic stem cells differentiate into lymphoid and myeloid progenitor cells. To develop into T cells, lymphoid progenitor cells migrate to the thymus, where T cell lineage commitment starts with the double negative (CD4-/CD8-) stage. The cells proceed through the double positive (CD4+/CD8+) stage, and end with the single positive stage, generating either nave CD4+ or nave CD8+ T cells. During the adaptive immune response, nave CD8+ T cells interact with antigen and activate, differentiating into effector T cells and potentially memory T cells after antigen clearance, while nave CD4+ T cells activated by antigen differentiate into helper T cells. B cells mature in the spleen and remain nave until they encounter antigen. Activated B cells differentiate into plasma B cells or memory B cells, or undergo an intermediate differentiation step and switch antibody classes to become germinal center B cells. T cell and B cell activation involves many sequential steps and the careful timing of cellular signaling pathways. Specific miRNAs play roles in fine-tuning the differentiation and activation of these essential immune cells, and dysregulation of B cell or T cell functions can cause immunodeficiencies or cancer. A set of controls present on each array enables data analysis using the ΔΔCT method of relative quantification, assessment of reverse transcription performance, and assessment of PCR performance. Using SYBR Green-based real-time PCR, the expression of a focused panel of miRNAs involved in T cell and B cell activation can be easily and reliably analyzed with this miScript miRNA PCR Array.

miScript miRNA PCR Arrays are intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

96-well, 384-well ( 4 x 96 ), and Rotor-Disc 100 formats are available.

See the complete miRNA PCR Array list

 

Functional miRNA Grouping How It Works Manual & Resources Reagents & Software
 
Pathway Source References     Gene Table

T-Cell Differentiation*:
Double Negative (CD4-/CD8-): let-7d-5p, let-7e-5p, miR-10a-5p, miR-126a-3p, miR-128-3p, miR-146b-5p, miR-17-5p, miR-181c-5p, miR-191a-5p, miR-19a-3p, miR-199a-5p, miR-20a-5p, miR-20b-5p, miR-221-3p, miR-222-3p, miR-223-3p, miR-28-5p, miR-29c-3p, miR-30e-5p, miR-320-3p, miR-342-3p, miR-423-5p, miR-93-5p, miR-98-5p.
Double Positive (CD4+/CD8+): let-7b-5p, miR-181a-5p, miR-181b-5p, miR-181d-5p, miR-19b-3p.
CD4+ Nave: miR-132-3p, miR-146a-5p, miR-182, miR-184, miR-25-3p, miR-326-3p, miR-92a-3p, miR-96-5p.
CD8+ Nave: let-7a-5p, let-7c-5p, let-7f-5p, miR-101b-3p, miR-103-3p, miR-130b-3p, miR-139-5p, miR-142-3p, miR-150-5p, miR-16-5p, miR-26a-5p, miR-26b-5p, miR-29b-3p, miR-30b-5p, miR-30c-5p, miR-30d-5p, miR-378a-3p.
CD8+ Effector: miR-147, miR-18a-5p, miR-27a-3p, miR-27b-3p.
CD8+ Memory: let-7i-5p, miR-106b-5p, miR-142-5p, miR-15b-5p, miR-17-5p, miR-21-5p, miR-23a-3p, miR-23b-3p, miR-24-3p, miR-29a-3p, miR-31a-5p.

B-Cell Differentiation*:
Nave: let-7a-5p, let-7b-5p, let-7d-5p, let-7i-5p, miR-101a-3p, miR-132-3p, miR-142-3p, miR-142-5p, miR-150-5p, miR-181c-5p, miR-195-5p, miR-204-5p, miR-214-3p, miR-221-3p, miR-222-3p, miR-223-3p, miR-29b-3p, miR-30e-5p, miR-320-3p, miR-331-3p, miR-92a-3p.
Germinal Center: miR-103-3p, miR-106b-5p, miR-130b-3p, miR-132-3p, miR-15b-5p, miR-16-5p, miR-17-5p, miR-181a-5p, miR-181b-5p, miR-191a-5p, miR-19a-3p, miR-19b-3p, miR-210-3p, miR-23b-3p, miR-25-3p, miR-28-5p, miR-30d-5p, miR-93-5p, miR-98-5p.
Memory: miR-100-5p, miR-125b-5p, miR-145-5p, miR-146a-5p, miR-21-5p, miR-23a-3p, miR-24-3p, miR-26a-5p, miR-26b-5p, miR-27a-3p, miR-27b-3p, miR-29a-3p, miR-29c-3p, miR-30b-5p, miR-30c-5p, miR-34a-5p.

Differentially Expressed in Tregs: miR-100-5p, miR-125b-5p, miR-146a-5p, miR-181c-5p, miR-20b-5p, miR-21-5p, miR-31a-5p, miR-365-3p, miR-99a-5p.

T-Cell Activation: let-7e-5p, let-7i-5p, miR-106b-5p, miR-139-5p, miR-142-5p, miR-146a-5p, miR-146b-5p, miR-181a-5p, miR-181c-5p, miR-195-5p, miR-20b-5p, miR-214-3p, miR-223-3p, miR-23a-3p, miR-23b-3p, miR-25-3p, miR-26a-5p, miR-26b-5p, miR-27a-3p, miR-27b-3p, miR-29a-3p, miR-29b-3p, miR-29c-3p, miR-30a-5p, miR-30b-5p, miR-30e-5p, miR-320-3p, miR-342-3p, miR-346, miR-483-3p, miR-672-5p, miR-98-5p.

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Functional miRNA Grouping How It Works Manual & Resources Reagents & Software
 

What miScript miRNA PCR Arrays offer?
   Guaranteed Performance* - ready-to-use for gene expression analysis
   Time & Cost Saving - less than 30 min hands-on time to analyze 84 or 372 miRNA
   Simple Data Analysis - easy-to-use Excel-Based Data Analysis Template

Layout and Controls: miScript miRNA PCR Arrays are available in 96-well, 384-well, and Rotor-Disc 100 formats. Included on each array is a set of six normalization controls as well as controls to assess RNA recovery, reverse transcription performance, and PCR performance.

You can easily perform a PCR Array experiment in your own laboratory, or send your samples to us and take advantage of our PCR Array Gene Expression Analysis Services.

*: when using the Complete miRNA PCR Array System.

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Functional miRNA Grouping How It Works Manual & Resources Reagents & Software
 

User Manual

miScript miRNA PCR Array Handbook  (PDF)

White Paper

miRNA Profiling From Blood Challenges and Recommendations (PDF)

Data Analysis

Free miRNA PCR Array Data Analysis Software (Web & Excel based)

Required Reagents and Setup Instructions

Materials & Equipment Required for miScript miRNA PCR Products
miRNA PCR Array Instrument Setup Instructions & Protocol Files

Web Seminars

Attend a live on-line seminar hosted by an Applications Scientist about PCR

Technical Brochures

miScript PCR System Brochure (PDF)

Pathway Central

Pathway reviews and presentation-ready pathway maps

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Functional miRNA Grouping How It Works Manual & Resources Reagents & Software
 

The complete miScript miRNA PCR Array System Includes:

Data Analysis: miRNA PCR Array Data Analysis Software
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