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Apoptosis miRNA PCR Array

Human Mouse Rat Other Species
Rat Apoptosis miRNA PCR Array: MIRN-114Z
The Rat Apoptosis miScript miRNA PCR Array profiles the expression of 84 miRNAs that regulate programmed cell death by inhibiting pro-apoptotic and anti-apoptotic gene expression. This array provides researchers with a convenient way to quickly analyze the miRNAs most relevant to apoptosis. Apoptosis plays a critical role in normal biological processes requiring cell removal including differentiation, development, and homeostasis. Stress responses (such as heat shock, ischemia, unfolded proteins, and viral infection) cause badly damaged cells to undergo apoptosis. In cell culture, growth factor withdrawal and many known experimental compounds have a similar effect. An acquired defect in apoptosis activation often leads to uncontrolled cell growth, oncogenesis, and cancer. Ligand-bound tumor necrosis factor (TNF) receptors initiate apoptosis by recruiting FADD and other death domain adaptor proteins that then recruit and activate caspases. Environmental stresses trigger BCL2 protein oligomerization and insertion into the mitochondrial membrane, releasing APAF1 and other CARD family members that also oligomerize to recruit and activate caspases. Caspases promote a proteolytic cascade that degrades cellular protein targets, while the IAP protein family directly inhibits caspases. Besides these post-translational events, miRNAs provide another mechanism of regulating apoptosis by attenuating gene expression. This array represents miRNAs experimentally verified to regulate many pro- and anti-apoptotic genes and to stimulate or attenuate apoptosis upon their overexpression or inhibiton. Monitoring their expression helps determine the mechanisms behind programmed cell death in any model system and the propensity of a cell type to undergo apoptosis normally. A set of controls present on each array enables data analysis using the ΔΔCT method of relative quantification, assessment of reverse transcription performance, and assessment of PCR performance. Using SYBR® Green real-time PCR, the expression of a focused panel of miRNAs related to apoptosis can be easily and reliably analyzed with this miScript miRNA PCR Array.

miScript miRNA PCR Arrays are intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

96-well, 384-well ( 4 x 96 ), and Rotor-Disc 100 formats are available.

See the complete miRNA PCR Array list


Functional miRNA Grouping How It Works Manual & Resources Reagents & Software
Pathway Source References     Gene Table

Anti-Apoptotic: miR-106b-5p, miR-133a-3p, miR-141-3p, miR-181a-5p, miR-183-5p, miR-186-5p, miR-21-5p, miR-210-3p, miR-214-3p, miR-25-3p, miR-30a-5p, miR-30d-5p, miR-338-5p, miR-378a-3p.

Either Anti-Apoptotic or Pro-Apoptotic: let-7a-5p, let-7e-5p, miR-23a-3p, miR-24-3p, miR-29b-3p, miR-34b-5p, miR-34c-5p, miR-34c-3p.

Other: miR-152-3p, miR-200b-3p, miR-215, miR-338-3p.

Pro-Apoptotic: let-7c-5p, miR-1-3p, miR-128-3p, miR-133b-3p, miR-143-3p, miR-144-3p, miR-153-3p, miR-15b-5p, miR-16-5p, miR-181b-5p, miR-195-5p, miR-200c-3p, miR-203a-3p, miR-204-5p, miR-205, miR-206-3p, miR-212-3p, miR-224-5p, miR-26a-5p, miR-26b-5p, miR-27a-3p, miR-29a-3p, miR-29c-3p, miR-30c-5p, miR-30e-5p, miR-31a-5p, miR-34a-5p, miR-365-3p, miR-409a-3p, miR-449a-5p, miR-497-5p, miR-708-5p.

Targets Anti-Apoptotic Genes: let-7c-5p, miR-1-3p, miR-101a-3p, miR-122-5p, miR-133a-3p, miR-133b-3p, miR-194-5p, miR-204-5p, miR-542-3p, miR-7a-5p, miR-9a-5p.

Targets Both Anti-Apoptotic & Pro-Apoptotic Genes: let-7a-5p, miR-125a-5p, miR-125b-5p, miR-145-5p, miR-146a-5p, miR-153-3p, miR-15b-5p, miR-16-5p, miR-17-5p, miR-181a-5p, miR-181b-5p, miR-181c-5p, miR-181d-5p, miR-185-5p, miR-192-5p, miR-195-5p, miR-203a-3p, miR-20a-5p, miR-21-5p, miR-25-3p, miR-298-5p, miR-29a-3p, miR-29b-3p, miR-29c-3p, miR-30a-5p, miR-30b-5p, miR-30d-5p, miR-34a-5p, miR-351-5p, miR-451-5p, miR-497-5p, miR-503-5p, miR-92a-3p.

Targets Pro-Apoptotic Genes: miR-134-5p, miR-221-3p, miR-222-3p, miR-32-5p.

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Functional miRNA Grouping How It Works Manual & Resources Reagents & Software

What miScript miRNA PCR Arrays offer?
   Guaranteed Performance* - ready-to-use for gene expression analysis
   Time & Cost Saving - less than 30 min hands-on time to analyze 84 or 372 miRNA
   Simple Data Analysis - easy-to-use Excel-Based Data Analysis Template

Layout and Controls: miScript miRNA PCR Arrays are available in 96-well, 384-well, and Rotor-Disc 100 formats. Included on each array is a set of six normalization controls as well as controls to assess RNA recovery, reverse transcription performance, and PCR performance.

You can easily perform a PCR Array experiment in your own laboratory, or send your samples to us and take advantage of our PCR Array Gene Expression Analysis Services.

*: when using the Complete miRNA PCR Array System.

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Functional miRNA Grouping How It Works Manual & Resources Reagents & Software

User Manual

miScript miRNA PCR Array Handbook  (PDF)

White Paper

miRNA Profiling From Blood – Challenges and Recommendations (PDF)

Data Analysis

Free miRNA PCR Array Data Analysis Software (Web & Excel based)

Required Reagents and Setup Instructions

Materials & Equipment Required for miScript miRNA PCR Products
miRNA PCR Array Instrument Setup Instructions & Protocol Files

Web Seminars

Attend a live on-line seminar hosted by an Applications Scientist about PCR

Technical Brochures

miScript PCR System Brochure (PDF)

Pathway Central

Pathway reviews and presentation-ready pathway maps

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Functional miRNA Grouping How It Works Manual & Resources Reagents & Software

The complete miScript miRNA PCR Array System Includes:

Data Analysis: miRNA PCR Array Data Analysis Software
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