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Materials and Equipment Required for miRNA Analysis

The following is required to analyze miRNA expression using miScript miRNA PCR Arrays:

miRNA sample isolation kits (select one according to your sample type)
       miRNeasy Mini Kit (217004)
       miRNeasy 96 Kit (217061)
       miRNeasy Micro Kit (217084)
       miRNeasy FFPE Kit (217504)
       miRNeasy Serum/Plasma Kit (217184)
       PAXgene« Tissue miRNA Kit (766134)
       PAXgene« Blood miRNA Kit (763134)

miRNA cDNA synthesis (two sizes available)
       miScript II Reverse Transcription Kit for 12 samples (218160)
       miScript II Reverse Transcription Kit for 50 samples (218161)

miRNA PCR arrays
       miRNome miScript miRNA PCR Arrays for miRNome profiling
       miScript miRNA PCR Arrays for biologically relevant, focused assay panels
       miScript miRNA HC PCR Arrays for an economical alternative to miRNome profiling
       Custom miScript miRNA Arrays for almost any configuration of assays
              
Remember to select the correct plate format for your qPCR instrument

miRNA preamplification reagents for limiting samples (two sizes available)
      miScript PreAMP PCR Kit for 12 samples (331451)
      miScript PreAMP PCR Kit for 60 samples (331452)
      miScript PreAMP miRNome Primer Mix
      miScript PreAMP Pathway Primer Mix
      miScript PreAMP Pathway HC Primer Mix

Real-time PCR master Mix (two sizes available)
       miScript SYBR Green PCR Kit, 200 x 50 Ál reactions (218073)
            Enough reagents for:
                4 x 96-well arrays (110 Ál residual) or
                2 x 384-well pathway arrays (1210 Ál residual) or
                2 x 384-well HC/miRNome arrays (1510 Ál residual) or
                5 x Rotor-Disc 100 arrays (110 Ál residual)
       miScript SYBR Green PCR Kit, 1000 x 50 Ál reactions (218075)
            Enough reagents for:
                20 x 96-well arrays (0 Ál residual) or
                12 x 384-well pathway arrays (1100 Ál residual) or
                13 x 384-well HC/miRNome arrays (850 Ál residual) or
                25 x Rotor-Disc 100 arrays (0 Ál residual)

Required Equipment and Reagents

       Thin-walled, DNase-free, RNase-free PCR tubes (for 20 Ál reactions)
       Nuclease-free pipet tips and tubes
       Single channel pipettors
       Multichannel pipettor
       Microcentrifuge
       Thermal cycler, heating blocks, or water baths (capable of reaching 95░C)
       Real-time PCR instrument and instrument specific details are available [here].

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