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RT² Profiler™ PCR Array Layout

RT² Profiler PCR Array      PCR Array 384HT

Wells A1 through G12 contain primers for genes from the same biological pathway or the same disease state or genes that are otherwise functionally related.
Wells H1 through H5 contain a housekeeping gene panel to normalize PCR Array data.
The product information included with each cataloged PCR Array contains a list of the pathway-focused and housekeeping genes on the array.

Well H6 contains a Genomic DNA Control (GDC) primer set that specifically detects non-transcribed genomic DNA contamination with a high level of sensitivity.

Wells H7 through H9 contain replicate Reverse Transcription Controls (RTC) to test the efficiency of the RT² First Strand Synthesis Kit (330401) reaction with a primer set to detect template synthesized from the kit's built-in external RNA control.

Wells H10 through H12 contain replicate Positive PCR Controls (PPC) to test the efficiency of the polymerase chain reaction itself using a pre-dispensed artificial DNA sequence and the primer set that detects it.

The two sets of replicate control wells (RTC and PPC) also test for inter-well, intra-plate consistency.

The 96 real-time assays found in the 96-well PCR plate-based arrays are located in wells 1 through 96 of the 100-well Rotor-Gene Q / Rotor-Gene 6000 disc (plate A1-A12 → Disc 1-12, plate B1-B12 → Disc 13-24, etc.). To maintain data analysis compatibility, wells 97-100 do not contain real-time assays but will contain master mix to account for weight balance.

The 384-well format of the PCR Arrays includes four replicates of the same 96-well format, in which a two-by-two set of wells (wells labeled 1 - 4 in gray above) contains the same primer sets represented by the 96-well designations.

Custom PCR Arrays have your specified layout, and the product information enclosed with the array reiterates that layout and the genes included.

PCR Array 384HT

Wells A1 through P10 (1-370) each contain a real-time PCR assay for a gene from the same biological pathway or disease state panel of 370 genes represented by the complete array. Wells P11 through P15 contain a housekeeping gene panel to normalize PCR Array data. The product information included with each cataloged PCR Array contains a list of the pathway-focused and housekeeping genes on the array.

Wells P16 through P18 contain replicate Genomic DNA Control (GDC) primer sets that specifically detect non-transcribed genomic DNA contamination with a high level of sensitivity.

Wells P19 through P21 contain replicate Reverse Transcription Controls (RTC) to test the efficiency of the RT² First Strand Kit (330401) reaction with a primer set to detect template synthesized from the kit's built-in external RNA control.

Wells P22 through P24 contain replicate Positive PCR Controls (PPC) to test the efficiency of the polymerase chain reaction itself using a pre-dispensed artificial DNA sequence and the primer set that detects it.

The three sets of replicate control wells (GDC, RTC, and PPC) also test for inter-well, intra-plate consistency.

Custom PCR Arrays have your specified layout, and the product information enclosed with the array reiterates that layout and the genes included.