RT² Profiler™ PCR Array System and Protocol Guide

SABiosciences' RT² Profiler PCR Arrays serve as a complete system for pathway-focused gene expression analysis for cell and tissue samples (0.1 - 5 µg total RNA), small samples (< 25 ng total RNA), and FFPE samples. Together with RT² reagents, PCR Arrays are guaranteed to provide you with accurate and reliable gene expression data for each and every experiment. This sample guide highlights the reagents and protocols necessary for the high performance of RT² Profiler Arrays. See the basic protocol.

RT² Profiler PCR Arrays have been optimized for RNA prepared from the following samples:

• Fresh and frozen cells & tissues
• Small samples (FNA/FNB, LCM, FACS Cells, Stem Cells)
• FFPE samples

Learn how to prepare your sample and analyze your samples on RT² Profiler PCR arrays by selecting your sample from below.

Cells & Tissues (0.1-5 µg RNA)

FFPE Samples (0.1-5 µg FFPE RNA)

Small Samples (1-100 ng RNA)

Regular Sample (0.1-5 µg total RNA) PCR Array Analysis How RT² Profiler PCR Arrays Work Protocol: RT² Profiler PCR Array User Manual Instrument Set Up Guides Required Reagents & Tools: RT² Profiler PCR Arrays (Select from complete list of PCR Arrays) RT² First-Stand cDNA Synthesis Kit RT² qPCR SYBR Green Master Mixes RT² FAST qPCR SYBR Green Master Mixes (for Rotor-Gene Q & Rotor-Gene 6000) RT² PCR Data Analysis Software (Free) Optional Reagents: RT² qRT-PCR Primer Assays RNeasy Mini Kit RNase-Free DNase Kit   Back to Top

Cells & Tissues (0.1-5 µg RNA)

FFPE Samples (0.1-5 µg FFPE RNA)

Small Samples (1-100 ng RNA)

FFPE Sample PCR Array Analysis How RNeasy FFPE RNA Extraction and RT² PreAMP Work with RT² Profiler PCR Arrays Protocol: RNeasy FFPE Kit Handbook RT² PreAMP cDNA Synthesis Kit User Manual RT² Profiler PCR Array User Manual Instrument Set Up Guides Required Reagents & Tools: RT² Profiler PCR Arrays (Select from complete list of PCR Arrays) RT² PreAMP cDNA Synthesis Kit RT² PreAMP Primer Mix (Select to match with your PCR Array) RT² qPCR SYBR Green Master Mixes RT² FAST qPCR SYBR Green Master Mixes (for Rotor-Gene Q & Rotor-Gene 6000) RT² PCR Data Analysis Software (Free) Optional Reagents: RNeasy FFPE Kit   Back to Top

Cells & Tissues (0.1-5 µg RNA)

FFPE Samples (0.1-5 µg FFPE RNA)

Small Samples (1-100 ng RNA)

Small Sample (1-100 ng total RNA) PCR Array Analysis How Nano PreAMP Works with RT² Profiler PCR Arrays Protocol: RT² PreAMP cDNA Synthesis Kit User Manual RT² Profiler PCR Array User Manual Instrument Set Up Guides Required Reagents & Tools: RT² Profiler PCR Arrays (Select from complete list of PCR Arrays) RT² PreAMP cDNA Synthesis Kit RT² PreAMP Primer Mix RT² qPCR SYBR Green Master Mixes RT² FAST qPCR SYBR Green Master Mixes (for Rotor-Gene Q & Rotor-Gene 6000) RT² PCR Data Analysis Software (Free) Optional Reagents: RNeasy Micro Kit Back to Top