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RT² Profiler™ PCR Array System and Protocol Guide

SABiosciences' RT² Profiler PCR Arrays serve as a complete system for pathway-focused gene expression analysis for cell and tissue samples (0.1 - 5 µg total RNA), small samples (< 25 ng total RNA), and FFPE samples. Together with RT² reagents, PCR Arrays are guaranteed to provide you with accurate and reliable gene expression data for each and every experiment. This sample guide highlights the reagents and protocols necessary for the high performance of RT² Profiler Arrays. See the basic protocol.

RT² Profiler PCR Arrays have been optimized for RNA prepared from the following samples:

  • Fresh and frozen cells & tissues
  • Small samples (FNA/FNB, LCM, FACS Cells, Stem Cells)
  • FFPE samples

Learn how to prepare your sample and analyze your samples on RT² Profiler PCR arrays by selecting your sample from below.

Cells & Tissues
(0.1-5 µg RNA)
FFPE Samples
(0.1-5 µg FFPE RNA)
Small Samples
(1-100 ng RNA)
 

Regular Sample (0.1-5 µg total RNA) PCR Array Analysis

How RT² Profiler PCR Arrays Work

Protocol:

Required Reagents & Tools:

Optional Reagents:

 

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Cells & Tissues
(0.1-5 µg RNA)
FFPE Samples
(0.1-5 µg FFPE RNA)
Small Samples
(1-100 ng RNA)
 

FFPE Sample PCR Array Analysis

How RNeasy FFPE RNA Extraction and RT² PreAMP Work with RT² Profiler PCR Arrays

Protocol:

Required Reagents & Tools:

Optional Reagents:

 

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Cells & Tissues
(0.1-5 µg RNA)
FFPE Samples
(0.1-5 µg FFPE RNA)
Small Samples
(1-100 ng RNA)
 

Small Sample (1-100 ng total RNA) PCR Array Analysis

How Nano PreAMP Works with RT² Profiler PCR Arrays

Protocol:

Required Reagents & Tools:

Optional Reagents:

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