Neuroblastoma RAS viral (v-ras) oncogene homolog Detailed info
The N-ras oncogene is a member of the RAS gene family. It is mapped on chromosome 1, and it is activated in HL60, a promyelocytic leukemia line. The order of nearby genes is as follows: cen--CD2--NGFB--NRAS--tel. The mammalian ras gene family consists of the harvey and kirsten ras genes (c-Hras1 and c-Kras2), an inactive pseudogene of each (c-Hras2 and c-Kras1) and the N-ras gene. They differ significantly only in the C-terminal 40 amino acids. These ras genes have GTP/GDP binding and GTPase activity, and their normal function may be as G-like regulatory proteins involved in the normal control of cell growth. Mutations which change amino acid residues 12, 13 or 61 activate the potential of N-ras to transform cultured cells and are implicated in a variety of human tumors. The N-ras gene specifies two main transcripts of 2Kb and 4.3Kb. The difference between the two transcripts is a simple extension through the termination site of the 2Kb transcript. The N-ras gene consists of seven exons (-I, I, II, III, IV, V, VI). The smaller 2Kb transcript contains the VIa exon, and the larger 4.3Kb transcript contains the VIb exon which is just a longer form of the VIa exon. Both transcipts encode identical proteins as they differ only the 3' untranslated region. The sequence of the shorter 2Kb transcript is presented here. The 4.3 Kb transcript sequence is not available.
Please click here to view the Band Size and Reference Positions information.
Band Size (bp): 87
Reference Position: 802
The Reference Position is a position contained within the sequence of the amplicon relative to the start of the relevant RefSeq sequence.
The RefSeq Accession number refers to the representative sequence used to design the enclosed primers.
These primers can also generate amplicons from the following splice variants:
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12.5
µl
RT² SYBR Green qPCR Master Mix
10.5
µl
H2O
1.0
µl
RT² First Strand cDNA (template)
1.0
µl
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25.0 µl final volume
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Assay-Specific
Pre-Mix
12.5
µl
RT² SYBR Green qPCR Master Mix
10.5
µl
H2O
1.0
µl
gene-specific RT² qPCR Primers
24.0 µl final volume
For each 25-µl PCR reaction,
mix the following in a PCR tube
24.0
µl
Assay-Specific Pre-Mix
1.0
µl
RT² First Strand cDNA (template)
25.0 µl final volume
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Acquisition.
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Complete Array List
For each 25-µl PCR reaction,