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qBiomarker SYBR Green Mastermixes

Master Mixes for SYBR® Green Real-time PCR

  • Available with ROX, Fluorescein
  • Chemically modified HotStart
SYBR® Green Master Mix
  • Superior Performance
  • Convenient
  • Cost-effective

Recommended for:

ABI, Bio-Rad, Agilent/Stratagene, Eppendorf, TaKaRa

FAST SYBR® Green Master Mix
  • Shorter Protocols
  • Higher Sensitivity
  • Sharper Dissociation Curves

Recommended for:

QIAGEN Rotor-Gene Q

Benefits of qBiomarker Mastermix:

  • Accuracy: Guarantees single locus-specific product with minimal primer dimers.
  • Reliability: Maximizes amplification efficiencies.
  • Convenience: Specially formulated all-in-one mixes that match the instrument in your lab.

Learn how we developed the qBiomarker Mastermix

Unique Elements of qBiomarker Mastermix
Master mix quality plays a critical role in the performance of real-time PCR. The key to obtaining accurate results is a tightly-controlled hot start enzyme that eliminates the amplification of primer dimers and other secondary products. Antibody-based technologies have worked well in the past for most PCR applications. However, these enzymes too often "leak" and synthesize too many non-specific amplicons to meet the more rigorous and demanding standards set by QIAGEN for real-time detection of message transcripts. After years of experience in designing and experimentally testing thousands of PCR primers for entire genomes, QIAGEN has developed a qPCR master mix formulation that provides the most accurate real-time PCR results.

Unique HotStart Polymerase and Buffer Components
The qBiomarker Mastermix from QIAGEN offer superior specificity and amplification efficiency thanks to our high-quality chemically-modified HotStart Taq DNA polymerase. Hot start enzymes found in other sources of master mix, such as the antibody-based technologies, still retain residual activity in their "inactive" state at low temperature. Under these conditions, non-specific priming events are extended to form templates which are then amplified into non-specific products that artificially contribute to signal intensity, resulting in unnecessary false positive results. Our tightly controlled HotStart enzyme lacks any activity before being heated and therefore minimizes the amplification of non-specific PCR products.
The qBiomarker Mastermix formulations also include other proprietary chemical components that significantly minimize primer dimer formation ensuring high amplification efficiencies for even the most difficult to amplify genes.

All-in-One Master Mixes: Instrument Reference Dyes Included
The qBiomarker Mastermix are tailor-made and optimized for the specific real time system in your lab. These cost-effective all-in-one PCR master mixes not only contain optimized buffers, nucleotides, and our high-quality HotStart DNA polymerase, but they also include the appropriate reference dye specifically used by individual real time systems to normalize their optics. Just add your primer pairs and templates, and you're ready for PCR.

Complete your copy number experiments with qBiomarker Copy Number Assays, qBiomarker Copy Number PCR Arrays, with the appropriate qBiomarker Mastermix tailor-made to the instrument in your laboratory.

SYBR® is a registered trademark of Molecular Probes. ABI® is a registered trademark of Applied Biosystems. iCycler® is a registered trademark of BioRad Laboratories, Inc. SmartCycler® is a registered trademark of Cepheid Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchasers own internal research. No other patent rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.