qBiomarker SYBR Green Mastermixes
Master Mixes for SYBR® Green Real-time PCR
- Available with ROX, Fluorescein
- Chemically modified HotStart
|SYBR® Green Master Mix
- Superior Performance
ABI, Bio-Rad, Agilent/Stratagene, Eppendorf, TaKaRa
|FAST SYBR® Green Master Mix
- Shorter Protocols
- Higher Sensitivity
- Sharper Dissociation Curves
QIAGEN Rotor-Gene Q
Benefits of qBiomarker Mastermix:
- Accuracy: Guarantees single locus-specific product with minimal primer
- Reliability: Maximizes amplification efficiencies.
- Convenience: Specially formulated all-in-one mixes that match the
instrument in your lab.
Learn how we developed the qBiomarker Mastermix
Unique Elements of qBiomarker Mastermix
Master mix quality plays a critical role in the performance of real-time PCR.
The key to obtaining accurate results is a tightly-controlled hot start enzyme
that eliminates the amplification of primer dimers and other secondary products.
Antibody-based technologies have worked well in the past for most PCR
applications. However, these enzymes too often "leak" and synthesize
too many non-specific amplicons to meet the more rigorous and demanding
standards set by QIAGEN for real-time detection of message transcripts. After
years of experience in designing and experimentally testing thousands of PCR
primers for entire genomes, QIAGEN has developed a qPCR master mix formulation
that provides the most accurate real-time PCR results.
Unique HotStart Polymerase and Buffer Components
The qBiomarker Mastermix from QIAGEN offer superior specificity and
amplification efficiency thanks to our high-quality chemically-modified HotStart
Taq DNA polymerase. Hot start enzymes found in other sources of master mix, such
as the antibody-based technologies, still retain residual activity in their
"inactive" state at low temperature. Under these conditions,
non-specific priming events are extended to form templates which are then
amplified into non-specific products that artificially contribute to signal
intensity, resulting in unnecessary false positive results. Our tightly
controlled HotStart enzyme lacks any activity before being heated and therefore
minimizes the amplification of non-specific PCR products.
The qBiomarker Mastermix formulations also include other proprietary chemical
components that significantly minimize primer dimer formation ensuring high
amplification efficiencies for even the most difficult to amplify genes.
All-in-One Master Mixes: Instrument Reference Dyes Included
The qBiomarker Mastermix are tailor-made and optimized for the specific real
time system in your lab. These cost-effective all-in-one PCR master mixes not
only contain optimized buffers, nucleotides, and our high-quality HotStart DNA
polymerase, but they also include the appropriate reference dye specifically
used by individual real time systems to normalize their optics. Just add your
primer pairs and templates, and you're ready for PCR.
Complete your copy number experiments with qBiomarker Copy Number Assays,
qBiomarker Copy Number PCR Arrays, with the appropriate qBiomarker Mastermix
tailor-made to the instrument in your laboratory.
SYBR® is a registered trademark of Molecular Probes. ABI® is a registered
trademark of Applied Biosystems. iCycler® is a registered trademark of BioRad
Laboratories, Inc. SmartCycler® is a registered trademark of Cepheid Purchase
of this product includes an immunity from suit under patents specified in the
product insert to use only the amount purchased for the purchasers own internal
research. No other patent rights are conveyed expressly, by implication, or by
estoppel. Further information on purchasing licenses may be obtained by
contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre
Drive, Foster City, California 94404, USA.