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Cignal Finder Array - Tube Format
The tube format of the Cignal Finder Arrays are delivered in 12-tube strips,
along with important negative and positive controls. The assays are used right
out of the box for the transfection or reverse transfection of the reporter
assays into your cell lines of interest.
Simple Procedure:
- Transfect Cignal Reporter Assays and test nucleic acids into cells
- Treat with protein, peptide, or small molecule of interest
- Perform reporter quantitation using luciferase activity assays
All reporter assays are based on dual-luciferase technology. Each reporter
consists of a mixture of a pathway-focused transcription factor-responsive
firefly luciferase construct and a constitutively expressing Renilla luciferase
construct.
Dual-luciferase results are calculated for each transfectant. The change in
the activity of each signaling pathway is determined by comparing the normalized
luciferase activities of the reporter in treated versus untreated transfectants.
The identically treated negative control serves as a specificity control. The
positive control serves as a control for transfection efficiency, by monitoring
GFP expression, as well as a positive control for both the firefly and Renilla
luciferase assays.
Cignal Array Example Experiments:
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