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Development 10-pathway Reporter Arrays

Luciferase (Tubes) Luciferase (Plates) Lentiviral Luciferase
 
Development 10-pathway Reporter Luciferase Kit: CCA-003L (Tube Format)

The Development Reporter Array is the first commercial reporter array that allows for a comprehensive measurement of ten development-related signaling pathways. By screening 10 pathway activities simultaneously, scientists can identify relevant pathways and exclude irrelevant pathways from further analysis. Therefore, Cignal Finder Arrays pinpoint the pathways that are regulated by a specific gene or drug. The tube format allows scientists to customize the concentrations of reporter used and the experimental design.

Tube Pathway  Transcription Factor 
1 Notch RBP-Jκ
2 Wnt TCF/LEF
3 Myc/Max Myc/Max
4 NFκB NFκB
5 TGFβ SMAD2/SMAD3/SMAD4
6 Cell Cycle/pRb-E2F E2F/DP1
7 C/EBP C/EBP
8 cAMP/PKA CREB
9 MAPK/ERK Elk-1/SRF
10 MAPK/JNK AP-1
11 Cignal negative control  
12 Cignal positive control  

 

Kit Components How It Works Manual & Resources Related products
 
Component  Specification  Concentration and total volume
Each of the 10 Reporter Assays A mixture of an inducible transcription factor responsive firefly luciferase reporter and constitutively expressing Renilla construct (40:1).
(100 ng/µl 100 µl)
Negative control A mixture of non-inducible firefly luciferase reporter and constitutively expressing Renilla construct (40:1). (100 ng/µl 100 µl)
Positive control A mixture of a constitutively expressing GFP construct, constitutively expressing firefly luciferase construct, and constitutively expressing Renilla luciferase construct (40:1:1). (100 ng/µl 100 µl)
 

Kit Components How It Works Manual & Resources Related Products
 

Cignal Finder Array - Tube Format
The tube format of the Cignal Finder Arrays are delivered in 12-tube strips, along with important negative and positive controls. The assays are used right out of the box for the transfection or reverse transfection of the reporter assays into your cell lines of interest.

Simple Procedure:

  • Transfect Cignal Reporter Assays and test nucleic acids into cells
  • Treat with protein, peptide, or small molecule of interest
  • Perform reporter quantitation using luciferase activity assays

All reporter assays are based on dual-luciferase technology. Each reporter consists of a mixture of a pathway-focused transcription factor-responsive firefly luciferase construct and a constitutively expressing Renilla luciferase construct.

Dual-luciferase results are calculated for each transfectant. The change in the activity of each signaling pathway is determined by comparing the normalized luciferase activities of the reporter in treated versus untreated transfectants.

The identically treated negative control serves as a specificity control. The positive control serves as a control for transfection efficiency, by monitoring GFP expression, as well as a positive control for both the firefly and Renilla luciferase assays.


Cignal Array Example Experiments:

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Kit Components How It Works Manual & Resources Related Products
 
User Manuals Cignal Finder™ 10-Pathway Reporter Arrays (PDF)
White Paper Cignal Reporter Assay Kit: A High Performance Tool for Assessing the Functions of Genes, Biologics and Small Molecule Compounds (PDF)
Web Seminar Attend a live on-line seminar hosted by an Application Scientist about Cignal Reporters
Technical Brochure Cignal Reporter Assay Kits Technical Brochure (PDF)
Power Point Cignal™ Cell-Based Assays for Rapidly Analyzing Signaling Pathway Activity (PDF)
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Kit Components How It Works Manual & Resources Related Products
 
Pathway reporters Complete list of signaling pathway reporters
Attractene Transfection Achieve high transfection efficiencies and minimal cytotoxicity in your transfections
10-Pathway Arrays Simultaneously measure the activities of ten (10) signaling pathways in a single experiment
shRNA Plasmids Pathway related gene knockdowns - Guaranteed 70% gene knockdown

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