QIAGEN Website    Quick Order    Online Seminar    Contact    My Account
Home  >  Products and Services  >  PCR Home  >  PCR Array  >  RNA QC Array

RT² RNA QC PCR Array

Use the RTē RNA QC PCR Array to Detect and Prevent:

  • Reverse transcription inhibition
  • PCR amplification inhibition
  • Genomic DNA contamination
  • False positive signals
  • Multi-peak dissociation curves

The optional RTē RNA QC PCR Arrays are designed to assess the quality of either human, mouse, or rat RNA samples before characterization with the RTē Profiler™ PCR Array as part of the complete PCR Array System. It contains a number of PCR controls that test for RNA integrity, inhibitors of reverse transcription and PCR amplification, genomic and general DNA contamination. Failure of any of these controls would otherwise confound SYBR Green based real-time PCR results by causing false negative or false positive results.

The RTē RNA QC PCR Arrays provide added confidence in your real-time PCR results whether you are new to gene expression analysis and need help with your RNA quality control or you are characterizing a large number of samples in a high-throughput manner and need controls for RNA quality consistency across your entire study. Use the RTē RNA QC PCR Array to test the quality of your RNA samples before wasting your master mix and PCR Arrays on potentially sub-standard samples.

The RTē RNA QC PCR Array controls include:

Element / Well  Controls For
Two housekeeping genes (HK1, HK2) RNA Integrity
Genomic DNA Control (GDC) Genomic DNA Contamination
No Reverse Transcription control (NRT) Genomic DNA Contamination
No Template Control (NTC) General Non-Specific DNA Contamination
Reverse Transcription Control (RTC) Reverse Transcription Inhibition
Positive PCR Control (PPC) PCR Amplification Inhibition

Please note that the RTē PCR Array First Strand Kit must be used to synthesize cDNA template from your total RNA in order to accurately interpret all control elements on the RT² RNA QC PCR Array. For more information, see the RTē RNA QC PCR Array User Manual [ here ] or the Information Sheet, listed below by catalog number, for the plate representing your species of interest.

RT² RNA QC PCR Array

Species Catalog Number
Human (Homo sapiens) PAHS-999Z
Mouse (Mus musculus) PAMM-999Z
Rat (Rattus norvegicus) PARN-999Z
Cow (Bos taurus) PABT-999Z
Fruit Fly (Drosophila melanogaster) PADM-999
Pig (Sus scrofa) PASS-999Z
Dog (Canis lupus familiaris) PAFD-999Z
Zebrafish (Danio rerio) PAZF-999Z
Rabbit (Oryctolagus cuniculus) PANZ-999Z
Chinese hamster ovary (CHO, Cricetulus griseus) cell PAJJ-999Z
Horse (Equus ferus caballus) PAEC-999Z
Chicken (Gallus gallus) PAGG-999Z
Rhesus macaque (Macaca mulatta) PAQQ-999Z

PRODUCT SUPPORT:

PCR Array System Pricing & Ordering [ here ]
Reagents & Equipment required for the PCR Array System [ here ]
Download the User Manual [ here ].
Read a White Paper [ here ].
View a Slide Presentation [ here ].
Attend a live Web Seminar [here].

Learn more about our other real-time RT-PCR technologies:
Gene-by-Gene Analyses with our RT-PCR Primers [ here ]
Instrument-Specific qPCR Master Mixes [ here ]
Other RT-PCR Accessories [ here ]