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Shelf Life: All reagents are
stable for 6 months after receipt if stored at the recommended temperature.
The GE2 and BC4 tubes included in this kit are shipped on dry ice and
must be stored at -20ºC upon receipt. When stored properly at -20ºC,
their quality is guaranteed for 6 months.
The foil sealing films, 96-well plate, compression mat, and reagent
reservoir can be stored at room temperature.
All reagents are stable for 6 months after receipt if stored at the
Considerations of RNA amount to be used:
The RT² HT First Strand Kit yields results with as little as as 25 ng
or as much as 5 µg total RNA per well reaction. However, the optimal amount
of starting material depends on the relative abundance of the transcripts of
interest. Lower abundance transcripts require more RNA; higher abundance
transcripts require less RNA. Greater amounts of input RNA yield greater
number of positive calls; that is, genes expressed in the linear dynamic range
of the method.