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RT² Microfluidics qPCR Reagent System

Catalog Number Product Description Price  
330431  For 96 samples for use with a Fluidigm® BioMark™ Chip
Effective March 8, 2013, now includes microfluidics EvaGreen® qPCR Master Mix in addition to all the reagents needed for cDNA synthesis and preamplification.

Description
The RT² Microfluidics qPCR Reagent System provides a rapid and convenient procedure for processing cDNA samples for RT² Profiler PCR Array analysis using the Fluidigm BioMark Real-Time Instrument with EvaGreen chemistry. Reagents are included for first-strand cDNA synthesis, genomic DNA elimination, and targeted preamplification, as well as a master mix for microfluidic real-time PCR that is specially optimized for use with RT² Profiler PCR Arrays. In total, this system allow simultaneous and easy parallel processing of samples for pathway-focused qPCR analysis on a single Fluidigm 96.96 Dynamic Array™ Integrated Fluidic Circuit. 

See the RT² Profiler PCR Array Handbook for special instructions for the use of this product with the H-format PCR arrays.

Fluidigm, BioMark and Dynamic Array are trademarks of Fluidigm Corporation. EvaGreen is a registered trademark of Biotium, Inc.

Product Contents
Cat. no.

RT˛ Microfluidics qPCR Reagent System

For ninety-six 20 µl first strand cDNA synthesis reactions (including gDNA removal, targeted preamplification and qPCR); Buffer GE2, BC4 Reverse Transcriptase Mix, 2x Microfluidics qPCR Master Mix, Side Reaction Reducer, and RT² PreAMP PCR Mastermix .

330431