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RT² SYBR® Green qPCR Mastermix

SYBR Green/ROX  SYBR Green/Fluorescein  SYBR Green
 
Cat. # Compatible Instruments Size Price
330500 
  • Bio-Rad CFX96, CFX384, Chromo4, Opticon 2
  • Roche LightCycler 480 (96-well & 384-well)
  • All other real-time instruments
2 Arrays (2.7 mL)
330502 12 Arrays (16.2 mL)
330503  24 Arrays (32.4 mL)
330501 4-pack 384-well Arrays
330509 2000 reactions (25 mL)
 

Description Protocol Components
 
The RT² SYBR® Green qPCR Mastermixes contain all of the optimized reagents and buffers needed for SYBR® Green based real-time polymerase chain reactions in all real-time PCR instrumentation including ABI® Bio-Rad® Stratagene® Roche, and Eppendorf® Each Mastermix includes real-time PCR buffer, a high-performance HotStart DNA Taq polymerase, nucleotides, and ROX® or Fluorescein reference dye if and when needed to normalize a specific instruments' optics. Simply add the Mastermix to PCR tubes along with your template and primers. The chemically-modified and tightly controlled HotStart enzyme uniquely provides more accurate SYBR® Green results by preventing the amplification of primer dimers and other non-specific products. These RT² SYBR® Green qPCR Mastermixes are best suited for real-time PCR applications using SYBR® Green based detection like the RT² Profiler™, RT² miRNA, EpiTectChip and EpiTectMethyl DNA Methylation PCR Arrays and qPCR Assays from SABiosciences.

RT² SYBR® Green qPCR Mastermixes, from the experts in high-performance quantitative PCR, provide:

Accuracy: Guaranteed single gene-specific products with minimal primer dimers
Reliability: Maximized amplification efficiencies
Convenience: Matched to the instrument in your lab with specially formulated all-in-one mixes

 

Description Protocol Components
 

Ensure that you do not contaminate the 2X real-time PCR cocktail by dividing into aliquots containing the amount of cocktail necessary for the number of reactions you are preparing each day. The rest of the cocktail should be kept in storage away from any sources of template DNA.

When using RT² qPCR Mastermix with RT² ProfilerTM PCR Arrays, RT² qPCR Primer Assays, EpiTect Methyl PCR Arrays/Assays, EpiTect ChIP PCR Arrays/Assays, please refer to the appropriate User Manual for reaction mixtures.

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Description Protocol Components
  Please check the kit components immediately after you receive this package. We are not responsible for any missing items not reported within two (2) business days upon receipt.

Contents:

Catalog #  Contents
330500 Two (2) tubes each containing 1.35 ml of 2X solution and enough for 100 standard 25-µl reactions
330502 Twelve (12) tubes each containing 1.35 ml of 2X solution and enough for one PCR Array
330503 Twenty-four (24) tubes each containing 1.35 ml of 2X solution and enough for one PCR Array
330501 Eight (8) tubes each containing 1.35 ml of 2X solution and enough for 4 384-well PCR Arrays
330509 One (1) bottle containing 25 ml of 2X solution and enough for 2000 standard 25-µl reactions

Storage Conditions:
The kit is shipped on dry ice or cold packs. For long-term storage, keep at -20 ºC. If entire volume is not be used all at once, divide into aliquots and store at -20 ºC. Avoid repeated freezing and thawing.

Shelf Life:
All reagents are stable for 6 months after receipt if stored at the recommended temperature.

SYBR® is a registered trademark of Molecular Probes, Inc.; SmartCycler® is a registered trademark of Cepheid; LightCycler® is a registered trademark of Roche Diagnostics; ABI® is a registered trademark of Applera Corporation; FAM™ is a trademark of Applera Corporation; ROX™ is a trademark of Applera Corporation; Bio-Rad® and iCycler® are registered trademarks of Bio-Rad Laboratories; Chromo4™ iQ™, MyiQ™ and Opticon™ are trademarks of Bio-Rad Laboratories; Stratagene® is a registered trademark of Stratagene Corporation; Eppendorf® and Mastercycler® are registered trademarks of Eppendorf AG; RT² and RT² Profiler™ are trademarks of SABiosciences Corporation.

Use of this product is covered by one of more of the following US patents and corresponding patent claims outside the US: 5,079,352, 5,789,224, 5,618,711, 6,127,155, 5,677,152 (claims 1 to 23 only), 5,773,258 (claims 1 and 6 only), 5,407,800, 5,322,770, 5,310,652, 5,994,056, 6,171,785, and claims outside the US corresponding to US Patent No. 4,889,818. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

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