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RT² SYBR® Green/Fluorescein FAST Master Mixes

ABI / Stratagene  BioRad All Others
 
Cat. # Product Description Size Price
330610 RT² SYBR® Green/Fluorescein FAST Master Mixes 2 Tubes (1.35 mL each)
200 reactions
330612 12 Tubes (1.35 mL each)
1200 reactions
330613 24 Tubes (1.35 mL each)
2400 reactions
330611 8 Tubes (1.35 mL each)
800 reactions
330619 2000 reactions (25 mL)
Instruments Supported:

BioRad® iCycler®, iQ™5, MyiQ™, MyiQ2

 

Description Protocol Component
 
The RT² SYBR Green/Fluorescein Fast master mix has been optimized to use a fast PCR protocol, without compromising specificity or sensitivity, using SYBR Green based detection on instrumentation using the Fluorescein reference dye, such as the:
  • BioRad® iCycler®, iQ™5, MyiQ™

The RT² SYBR/Fluorescein Green Fast master mix contains all of the reagents and buffers required for fast real-time polymerase chain reactions on instrumentation requiring the Fluorescein reference dye, including an improved proprietary real-time PCR buffer, a high-performance HotStart DNA Taq polymerase, nucleotides, SYBR Green dye, and the Fluorescein reference dye needed to normalize the instruments' optics. Simply add the master mix to PCR tubes or plates along with your template and primers. The chemically-modified and tightly controlled HotStart enzyme uniquely provides more accurate SYBR Green results by preventing the amplification of primer dimers and other non-specific products.

 

Description Protocol Components
 

Ensure that you do not contaminate the 2X real-time PCR cocktail by dividing into aliquots containing the amount of cocktail necessary for the number of reactions you are preparing each day. The rest of the cocktail should be kept in storage away from any sources of template DNA.

When using RT² qPCR Mastermix with RT² ProfilerTM PCR Arrays, RT² qPCR Primer Assays, EpiTect Methyl PCR Arrays/Assays, EpiTect ChIP PCR Arrays/Assays, please refer to the appropriate User Manual for reaction mixtures.

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Description Protocol Components
 

Please check the kit components immediately after you receive this package. We are not responsible for any missing items not reported within two (2) business days upon receipt.

Please put the appropriate table below with the corresponding selection from above.

Catalog Number  Contents
330600 Two (2) tubes, each containing 1.35 mL of 2X solution and enough for 200 25-µl reactions
330602 Twelve (12) tubes, each containing 1.35 mL of 2X solution and enough for 1200 25-µl reactions
330603 Twenty-four (24) tubes, each containing 1.35 mL of 2X solution and enough for 2400 25-µl reactions
330601 Eight (8) tubes, each containing 1.35 mL of 2X solution and enough for 800 25-µl reactions
330609 One (1) bottle containing 25 ml of 2X solution and enough for 2000 25-µl reactions

Catalog Number  Contents
330610 Two (2) tubes, each containing 1.35 mL of 2X solution and enough for 200 25-µl reactions
330612 Twelve (12) tubes, each containing 1.35 mL of 2X solution and enough for 1200 25-µl reactions
330613 Twenty-four (24) tubes, each containing 1.35 mL of 2X solution and enough for 2400 25-µl reactions
330611 Eight (8) tubes, each containing 1.35 mL of 2X solution and enough for 800 25-µl reactions
330619 One (1) bottle containing 25 ml of 2X solution and enough for 2000 25-µl reactions

Catalog Number  Contents
330620 Two (2) tubes, each containing 1.35 mL of 2X solution and enough for 200 25-µl reactions
330622 Twelve (12) tubes, each containing 1.35 mL of 2X solution and enough for 1200 25-µl reactions
330623 Twenty-four (24) tubes, each containing 1.35 mL of 2X solution and enough for 2400 25-µl reactions
330621 Eight (8) tubes, each containing 1.35 mL of 2X solution and enough for 800 25-µl reactions
330629 One (1) bottle containing 25 ml of 2X solution and enough for 2000 25-µl reactions

Storage Conditions
The kit is shipped on dry ice or cold packs. For long-term storage, keep at -20ºC. If entire volume is not be used all at once, divide into aliquots and store at -20ºC. Avoid repeated freezing and thawing.

Shelf Life
All reagents are stable for 6 months after receipt if stored at the recommended temperature.

NOTICE TO PURCHASER: LIMITED LICENSE

Use of this product is covered by one of more of the following US patents and corresponding patent claims outside the US: 5,079,352, 5,789,224, 5,618,711, 6,127,155, 5,677,152 (claims 1 to 23 only), 5,773,258 (claims 1 and 6 only), 5,407,800, 5,322,770, 5,310,652, 5,994,056, 6,171,785, and claims outside the US corresponding to US Patent No. 4,889,818. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

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