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Online Seminars

SABiosciences is pleased to provide free, on-line educational resources covering relevant tools and techniques for gene and protein expression analysis, and cell-based gene function analysis. We offer live webinars, prerecorded and PDF formatted presentations.

See the instructions for the Web Seminars

September 2017
Monday
Tuesday
Wednesday
Thursday
Friday

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Nucleic acid purification on QIAcube

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Targeted RNA-seq for gene expression

Targeted RNA-seq for gene expression

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Introducing Unique Molecular Indices technology

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Targeted DNA-seq for mutation detection

12

Genotyping and microbial identification on Rotor-Gene Q

miRNA-seq in liquid biopsy

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RNAseq data analysis with Biomedical GW

RNAseq data analysis with Biomedical GW

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gDNA collection, storage, and purification

NGS in single-cell applications

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Practical hints for successful PCR

Practical hints for successful PCR

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Circulating biomarker isolation from liquid biopsy samples

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Sample QC and reproducibility

20

Biologically interpret RNAseq data with IPA

Biologically interpret RNAseq data with IPA

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gDNA isolation from solid tissue samples

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Circulating miRNA analysis with NGS

Single-cell RNA-seq - QIAscout & QIAseq FX Single Cell RNA Library Kit

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DNA methylation analysis on PyroMark Q48 Autoprep

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Critical factors for successful multiplex qPCR

Critical factors for successful multiplex qPCR

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Current Seminar Titles Available:

Focus Title
1.  Multiplex PCRCritical Factors for Successful Multiplex Real-Time PCR
2.  Targeted sequencing in cancer clinical researchTargeted sequencing in cancer clinical research
3.  NGS Live in 30 Days (GeneReader)NGS Live in 30 Days: Challenges of, and a solution to, implementing NGS
4.  Bioinformatics for non-bioinformaticiansBioinformatics for non-bioinformaticians in cancer clinical research
5.  NGS: Now integrated and connected with your lab operationsNGS: Now integrated and connected with your lab operations
6.  QIAseq and QIAscoutSingle-cell RNA-seq using live cells and microrafts – linking cell morphology to transcriptomics
7.  AutomationRotor-Gene Q: A Rapid, Automated Real-Time PCR Instrument for Genotyping and Microbial Identification with Excellent Efficiency, Reproducibility and Support
8.  AutomationDNA methylation analysis in a single day – the new PyroMark Q48 Autoprep
9.  ExosomesCirculating biomarkers – what matters for the isolation of biomarkers from liquid biopsy samples?
10.  QIAseq NGSCirculating miRNA and RNA identification for biomarker assessment
11.  QuantiFERON®-TB Gold PlusWhat’s the ‘Plus’ in QuantiFERON®-TB Gold Plus? A webinar program for TB Controllers and public health professionals
12.  Liquid BiopsiesIn vivo selective-sorting of tumor suppressor miR-193a into tumor exosomes leads to promotion of tumor progression

Critical Factors for Successful Multiplex Real-Time PCR

Multiplex real-time PCR is a powerful tool for gene expression analysis, viral load monitoring, genotyping, and many other applications. The ability to amplify and detect several genomic DNA, cDNA, or RNA targets in the same reaction offers many benefits: • Conservation of precious samples – more quantification data per sample • Increased throughput – more targets analyzed per run on a cycler • Reliable results – no well-to-well variability due to co-amplification of internal control • Reduced costs – save time and reagents The QuantiFast Multiplex PCR and RT-PCR kits are optimized for reliable amplification of many different templates despite a high variability in abundance. Thus they enable successful amplification of multiple targets on the first attempt without optimization. This webinar explains the principles of the QIAGEN multiplex technologies and shows data demonstrating the exceptional multiplex real-time PCR performance of the QuantiFast Multiplex kits.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, September 28, 2017 at 9:30 AM Eastern    Status: Available Reserve
Thursday, September 28, 2017 at 1:00 PM Eastern    Status: Available Reserve

Targeted sequencing in cancer clinical research

In recent years, rapid advances in cancer genetics have revealed the importance of mutations in tumor cell proliferation and in determining the likely effectiveness of cancer therapies. As such, many cancer research laboratories recognize the value of interrogating the cancer genome in order to further understand the underlying molecular mechanisms driving tumorigenesis. In order to achieve this, labs are turning to Next-Generation Sequencing (NGS) to provide answers that will aid in the fight against cancer . Targeted sequencing using cancer-specific panels offers an efficient and effective way to capture the most relevant information. In this webinar you will learn how to apply current NGS technologies efficiently and effectively in cancer clinical research.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, October 4, 2017 at 9:30 AM Eastern    Status: Available Reserve

NGS Live in 30 Days: Challenges of, and a solution to, implementing NGS

Despite decreasing costs and increasing awareness of the important insights it can deliver, many barriers still exist to the broad adoption of next-generation sequencing (NGS) technology by smaller research labs. Obstacles to implementing NGS stemming from its inherent complexity, workflow fragmentation, cost obscurity, and protracted investment of time and resources, are often perceived as too great for many labs to overcome. However, as clinical cancer research expands beyond a small number of large academic centers, the implementation of this technology by all institutions is critical to moving research in precision medicine forward. Here we present the GeneReader NGS System, which has been rigorously tested and successfully implemented by multiple laboratories. Their experience can be used to help other labs considering the NGS technology.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, October 11, 2017 at 9:30 AM Eastern    Status: Available Reserve

Bioinformatics for non-bioinformaticians in cancer clinical research

As its cost and complexity continue to decrease, one of the main bottleneck in NGS lies in bioinformatics analysis and interpretation. Typically, specialized knowledge is required to customize a pipeline, fully understand the sequencing results, and ascribe meaning to variant findings. This is not possible in many laboratories which are battling resource and expertise challenges, while faced with the need to rapidly implement an NGS assay. Here we present the GeneReader NGS System comprising all elements required to lead you from sample preparation to generation of an actionable result. Including fully integrated bioinformatics for analysis and interpretation the workflow was specifically designed for labs of any size to conduct cancer clinical research.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, October 18, 2017 at 9:30 AM Eastern    Status: Available Reserve

NGS: Now integrated and connected with your lab operations

Are you choosing an NGS solution that offers connectivity with the rest of your laboratory operations? Imagine being able to track samples, record reagent lots, store results and manage workflows, all with the help of one easy software interface. In this webinar you will learn how to do this with the QIAGEN GeneRead Link Software.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, October 25, 2017 at 9:30 AM Eastern    Status: Available Reserve

Single-cell RNA-seq using live cells and microrafts – linking cell morphology to transcriptomics

"Dose-response studies and the analysis of involved genes and pathways is a key application in multiple research areas, especially in drug toxicity and cancer research. Single-cell analysis enables scientists to understand responses of individual cells by providing valuable information about gene expression variability. In this webinar, we will introduce a robust and easy-to-use single-cell RNA-seq workflow for studying cells selected for analysis based on morphological alterations after drug treatment. We will demonstrate an innovative microraft-based technology, the QIAscout, which is used with a standard microscope to isolate live single cells after being treated with PMA (phorbol 12-myristate 13-acetate). Transcriptome data from RNA-seq of responder versus non-responder cells analyzed using the QIAseq FX Single Cell RNA Library Kit will be presented. We will also provide further details on QIAGEN’s single-cell analysis workflow, which offers a simple and ideal platform to perform dose-response and time-course studies based on live cell morphology, allowing scientists to link cell biology to transcriptomics. If you’re working with live eukaryotic cells, join us and learn how you can quickly adapt this workflow to your lab."

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, September 25, 2017 at 1:00 PM Eastern    Status: Available Reserve

Rotor-Gene Q: A Rapid, Automated Real-Time PCR Instrument for Genotyping and Microbial Identification with Excellent Efficiency, Reproducibility and Support

"Molecular testing for the identification of bacterial and viral infections as well as gene mutations from raw livestock samples and medical research specimens is always a challenging task. It requires a multistep approach to optimize assays and to eliminate inhibitory effects in PCR amplification. Laboratory benchtop PCR assay setup procedures can be very labor intensive and unreliable, which is reflected in its prolonged assay time, poor reproducibility and increasing total assay costs. The need for automation has led to the development and introduction of robotic laboratory instruments, aiming to decrease operator errors and process time, and to increase process safety. We have developed a selection of robust, novel chemistries to prevent PCR crosstalk. We can successfully measure target abundance and fold change in real-time assays, and perform sub-genotyping using a fast, high-throughput and powerful High-Resolution Melting (HRM) statistical analysis program. We have also tested a liquid handler for quick PCR assay setup to eliminate manual steps and increase productivity. In this presentation, we will demonstrate these features and benefits with examples."

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, October 24, 2017 at 1:00 PM Eastern    Status: Available Reserve

DNA methylation analysis in a single day – the new PyroMark Q48 Autoprep

"Based on the sequencing-by-synthesis principle, Pyrosequencing is a highly flexible technology for rapid and quantitative analysis of any type of sequence variation. The real-time output delivers high-resolution sequence information, making Pyrosequencing highly suitable for various applications, particularly for DNA methylation quantification. Combined with the latest EpiTect Fast bisulfite conversion technology, the new PyroMark Q48 Autoprep can now provide highly automated methylation analysis in a single day. This webinar will focus on the following topics: • How bisulfite conversion can be improved for more reliable methylation results • How 5-hmC can be differentiated from 5-mC • Why Pyrosequencing is ideally suited for sensitive methylation analysis • What does “advanced” Pyrosequencing offer for methylation analysis • How the new PyroMark Q48 Autoprep streamlines the workflow for methylation analysis"

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, September 26, 2017 at 1:00 PM Eastern    Status: Available Reserve

Circulating biomarkers – what matters for the isolation of biomarkers from liquid biopsy samples?

"The analysis of circulating biomarkers in a patient’s blood holds significant potential for early disease detection and monitoring. Circulating DNA is the most commonly studied biomarker in the context of liquid biopsy research. However, in the last couple of years, free-circulating RNA has gained more attention for biomarker analysis and interpretation. In this webinar, we will discuss the different types of circulating RNA biomarkers studied in liquid biopsy samples. A special focus will be given to the challenges and solutions for isolating circulating RNA."

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, October 9, 2017 at 1:00 PM Eastern    Status: Available Reserve

Circulating miRNA and RNA identification for biomarker assessment

"Circulating RNA can serve as a powerful biomarker for patient stratification and assessment of overall health and prognosis. In this webinar, we will focus on using next-generation sequencing (NGS) for identification of miRNA and mRNAs from biofluids, including best practices for designing and executing a successful experiment. While RNA is vulnerable to degradation in serum/plasma due to the abundance of RNAseq and other degrading enzymes, mRNA and miRNA are protected within exosomes and other type of vesicles. Enrichment of exosomes from biofluids provides a supply of RNA biomarkers which can be profiled by NGS and further verified using targeted sequencing or qPCR. In this webinar, we will show how QIAGEN’s QIAseq miRNA Library Kit with Unique Molecular Indexes (UMIs) gives researchers several advantages compared to other small RNA sequencing kits. QIAseq miRNA delivers a gel-free workflow down to 1 ng of total RNA input and its proprietary chemistry minimizes ligation and increases the number of miRNA reads by actively blocking non-relevant, contaminating side products and RNAs. Other kits on the market may include a gel-free workflow or reduced ligation bias, but their protocols and methods fall short on delivering these promises. With the inclusion of UMIs in the QIAseq miRNA Library Kit, researchers are ensured that they have sequenced deep enough to cover the entire library and PCR and sequencing bias and errors are removed. In addition, we will address workflows for exosome mRNA discovery using stranded RNAseq and subsequent verification using targeted RNA-seq. QIAGEN’s exosome RNA discovery pipeline starts with a global view of the expressed RNA which is accurately identified using QIAGEN Biomedical Workbench’s tunable algorithm and verified using QIAseq Targeted RNA Panels which employ UMIs to ensure accurate quantification and adequate sequencing depth for each sample you run. Come listen to the RNA experts discuss the ‘next generation’ of sequencing-based workflows for biomarker detection and verification!"

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, September 25, 2017 at 9:30 AM Eastern    Status: Available Reserve
Monday, October 23, 2017 at 1:00 PM Eastern    Status: Available Reserve

What’s the ‘Plus’ in QuantiFERON®-TB Gold Plus? A webinar program for TB Controllers and public health professionals

"QuantiFERON-TB Gold Plus is now FDA approved and will soon launch in the US. The new QFT®-Plus features innovative CD8 technology that provides a more comprehensive picture of a patient’s immune response to TB antigens. Join Dr. Masae Kawamura for a dynamic discussion regarding scientific advancements in TB testing and CD8 technology, including a review of case scenarios applying the insight of QFT-Plus. This program is designed specifically to meet the needs of TB Controllers and others working in the Public Health sector. Presenter: Masae Kawamura, M.D. Senior Director, Medical & Scientific Affairs – TB Diagnostics, QIAGEN Dr. Kawamura is QIAGEN’s Senior Director of Scientific and Medical Affairs for TB diagnostics. She has been a TB clinician for more than 25 years and served as San Francisco’s TB Controller from 1996 to the end of 2011. Over a decade ago, under Dr. Kawamura's leadership to promote public health innovation and operations research, San Francisco became the first jurisdiction in the United States to implement interferon gamma release assay (IGRA) blood testing for TB as part of routine screening practice and eventually, contact investigation. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, October 2, 2017 at 1:00 PM Eastern    Status: Available Reserve
Wednesday, October 25, 2017 at 1:00 PM Eastern    Status: Available Reserve

In vivo selective-sorting of tumor suppressor miR-193a into tumor exosomes leads to promotion of tumor progression

"Cancer cell extracellular microvesicles (EVs) are thought to promote cancer progression through cell-cell communication, however, whether exosome release also has a biological effect on the exosome donor cell is poorly defined. In this webinar, we present data generated from three colon cancer models (mouse CT26 colon tumor model, CT26 liver metastasis model, and human SW620 colon tumor model) and from colon cancer patients, to address this question. We present evidence that colon tumor cells selectively sort tumor suppressor miRNA into tumor exosomes, leading to promotion of tumor progression without pressure. One specific exosomal miRNA, miR193a, was shown to target the 3’UTR of mRNA encoding for caprin1, upregulating Ccnd2 and c-myc, and ultimately causing cell cycle G1 arrest and cell proliferation repression. As a result, miR193a could serve as an excellent potential biomarker candidate for prognosis of colon cancer progression. To summarize, we define a new role for exosomes that allows donor tumor cells to grow under no pressure by oncoprotein-dependent sorting of tumor suppressor miRNA out of tumor cells. This finding provides a solid foundation for further investigation of whether intracellular machinery regulates tumor suppression versus oncogenic miRNA sorting in/out of exosomes. This approach may thus provide new therapeutic strategies for the treatment and/or prevention of cancer due to dysregulation of cellular miRNA homeostasis. Guest Speaker: Prof. Huang-Ge Zhang, MD, PhD Professor of Microbiology & Immunology, James Brown Cancer Center, University of Louisville, Louisville, KY"

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, October 16, 2017 at 9:30 AM Eastern    Status: Available Reserve
Monday, October 16, 2017 at 1:00 PM Eastern    Status: Available Reserve

Note: Viewers will be asked to register before viewing the previously recorded webinars.