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Online Seminars

SABiosciences is pleased to provide free, on-line educational resources covering relevant tools and techniques for gene and protein expression analysis, and cell-based gene function analysis. We offer live webinars, prerecorded and PDF formatted presentations.

See the instructions for the Web Seminars

December 2016
Monday
Tuesday
Wednesday
Thursday
Friday

1

Introduction to NGS

Tools for microbial detection and host-response analysis

2

5

QPCR Introduction

Circulating exosomes and exoRNA detection

6

Basics of sample prep in RNA research

Introduction to microRNA

7

Digital DNA-seq for cancer research

RNA-seq for biomarker discovery

NGS applications in oncology

8

Innovative NGS Library Construction Technology

Microbiome bioinformatics

9

12

Coding & noncoding RNA analysis using qPCR

Controls and novel solutions for real-time qPCR

13

gDNA collection, storage, and purification

Exosome profiling by RNA-seq Explorer solution

14

Analyzing fusion genes with NGS

Tips and tricks for end-point PCR

15

Advanced NGS library prep for challenging samples

CTC detection and characterization

16

19

20

21

22

23

26

27

28

29

30

Current Seminar Titles Available:

Focus Title
1.  PCR ArraysAdvanced Real-Time PCR Array Technology – Coding & Noncoding RNA Expression Analysis
2.  DNA sample isolationFundamental Concepts and Special Considerations in gDNA Isolation for Better Insight
3.  QuantiNova PCR kit The importance of controls and novel solutions for successful real-time qPCR
4.  End-point PCRBack to basics of PCR: comprehensive end-point PCR tips and tricks
5.  QIAseq RNAScanAnalyzing fusion genes with next-generation sequencing technology
6.  QIAseq Library PrepAdvanced NGS library prep for challenging samples
7.  AdnaTestsCTC Detection and molecular characterisation – Challenges and Solutions
8.  RNA-seq Explorer solutionTranscriptome analysis of Kupffer cells after uptake of pancreatic cancer exosomes reveal pathways and biological processes involved in metastatic progression

Advanced Real-Time PCR Array Technology – Coding & Noncoding RNA Expression Analysis

This webinar presents a simple and accurate real-time PCR system for relevant biological pathway- & disease-focused mRNA and long non-coding RNA (lncRNA) expression profiling. Learn about the stringent performance built into the technology to insure its sensitivity, specificity, reproducibility, and reliability. Application examples will be presented.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, December 12, 2016 at 9:30 AM Eastern    Status: Available Reserve

Fundamental Concepts and Special Considerations in gDNA Isolation for Better Insight

"How are your gDNA yields? Some sample types present special challenges in DNA purification and analysis. This webinar provides tips for a whole range of sample types that require special consideration. We will cover the following topics in detail: • The basic methods and challenges of gDNA purification • Working with DNA: good laboratory practice • Special considerations for challenging sample types • Performing DNA quality checks"

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, December 13, 2016 at 9:30 AM Eastern    Status: Available Reserve

The importance of controls and novel solutions for successful real-time qPCR

"The increasing demand for streamlined, monitored, and ultrafast qPCR procedures requires high-performance, real-time quantitative RT and PCR chemistries. Particularly procedures utilizing generic kits for gene expression analysis should include in-process safety measures to avoid variables and control accuracy of procedures and results. This webinar presents innovative solutions for one-step and two-step RT-PCR that significantly enhance performance and reliability in qRT-PCR. The new QuantiNova kit family offers a combination of various integrated safety features to remove variables and prevent artifacts. Internal Control RNA, removal of genomic DNA, room temperature set up capability for RT-PCR, and a built-in visual pipetting control verify accurate procedures, ensuring reliable gene expression profiling. This webinar explains the principles of the technologies and shows data demonstrating performance in qRT-PCR. Find out how you can verify accurate performance in qRT-PCR and improve your results."

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, December 12, 2016 at 1:00 PM Eastern    Status: Available Reserve

Back to basics of PCR: comprehensive end-point PCR tips and tricks

"Ever struggled with primer problems and annealing conditions? Ever wondered why your PCR didn’t work out quite right? Then join this webinar on end-point PCR Beginner’s Guide with tips and tricks you won’t find in the textbooks. At the end we also provide the link to download the updated PCR guide. Some of the highlights: • New feature on multiplex PCR for genotyping • New solutions for PCR fragments analysis • Extensive FAQs and troubleshooting tips"

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, December 14, 2016 at 1:00 PM Eastern    Status: Available Reserve

Analyzing fusion genes with next-generation sequencing technology

"Fusion genes are hybrid genes formed by the fusion of two separate genes. Translocation, interstitial deletion and chromosomal inversions are some of the genetic events that can lead to the formation of fusion genes. The occurrence of fusion genes and its implications in cancer have already been known, but the emergence of NGS technology – especially RNA sequencing – offers the potential to detect novel gene fusions. You can learn more about fusion genes and applying NGS to detect them at our upcoming webinar, presented by Raed Samara, Ph.D., QIAGEN’s Global Product Manager for NGS technologies. This webinar will cover: 1. Fusion genes: what they are and a historical perspective 2. Fusion gene detection: the current status 3. RNA sequencing vs. digital RNA sequencing 4. How to detect and accurately quantify novel fusion genes in your sample ? "

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, December 14, 2016 at 9:30 AM Eastern    Status: Available Reserve

Advanced NGS library prep for challenging samples

"Rapidly developing next-generation sequencing (NGS) technologies provide highly sensitive methods in discovering and characterizing the genetic information of a variety of samples. However, DNA samples are often limited in quantity, as well as compromised in quality. Such samples are not suitable for standard NGS library construction methods, which commonly require hundreds of nanograms of good-quality DNA. Examples of such challenging clinical samples include circulating DNA, laser capture microdissection (LCM) samples, formalin-fixed paraffin-embedded (FFPE) samples, ancient DNA and chromatin immunoprecipitation (ChIP) samples. In this webinar, we describe the measures that should be taken into consideration while sequencing such challenging samples. We will also present methods that can be used to optimize library construction to efficiently convert small amounts of DNA samples into sequencing libraries. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, December 15, 2016 at 1:00 PM Eastern    Status: Available Reserve

CTC Detection and molecular characterisation – Challenges and Solutions

Circulating Tumor Cells (CTCs) have been extensively explored as circulating biomarkers in various cancers. Due to their rarity, heterogeneity and stem cell-like properties, detecting and profiling CTCs from blood samples is very challenging. In this webinar, Dr. Siegfried Hauch will introduce the well-known AdnaTests, which uses the Combination of Combinations Principle (COCP) to enable enriching and detecting CTCs in whole blood with high specificity and sensitivity, and how to overcome challenges in CTC enrichment and detection. The AdnaTests combine an immunomagnetic capturing method that increases purity, and is followed by molecular profiling of the captured CTCs. In addition, leukocyte contamination is another issue in CTCs detection and may lead to false positive results due to illegitimate expression of target genes or false interpretation. The AdnaWash is developed to reduce leukocyte contamination to such a level that whole gene panels can be analyzed while maintaining the required specificity and sensitivity.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, December 15, 2016 at 9:30 AM Eastern    Status: Available Reserve

Transcriptome analysis of Kupffer cells after uptake of pancreatic cancer exosomes reveal pathways and biological processes involved in metastatic progression

Pancreatic cancer is one of the most lethal malignancies with a poor prognosis. Understanding the mechanisms of tumor progression is therefore essential. Liquid biopsies are non-invasive methods for diagnostics to detect early stage cancer resulting in more successful treatment. One liquid biopsy technique is the detection of RNA from tumor- derived exosomes. These exosomes participate in generating a metastatic niche. Using QIAGEN Bioinformatics solutions with a publicly available dataset we have examined the transcriptome of Kupffer cells after uptake of pancreatic tumor-derived exosomes that induce the formation of a liver metastatic niche and analyzed the pathways and biological processes involved in this process.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, December 13, 2016 at 1:00 PM Eastern    Status: Available Reserve

Note: Viewers will be asked to register before viewing the previously recorded webinars.