SABiosciences is pleased to provide free, on-line educational resources covering relevant tools and techniques for gene and protein expression analysis, and cell-based gene function analysis. These presentations can also help you get started with our pathway-focused and genome-wide technologies. Choose from:

Web Seminars:

SABiosciences offers free, on-line seminars covering relevant tools and techniques for gene and protein expression analysis, and cell-based gene function analysis. Attendance is free, but we do ask you to register with us. These educational seminars require just Internet access (we use the WebEx conferencing service) and a TOLL-FREE phone call to hear the presentation and ask questions. After registering, you will receive confirmation e-mail, then a reminder e-mail on the morning of the seminar. We rotate the seminar topics and update the schedule, so please check back periodically for new topics and new dates.

Current Seminar Titles Available:

Focus	Title
1.  Custom PCR Arrays	Personalize a PCR Array for Your Genes of Interest
2.  PCR Array	PCR Array Data Analysis Tutorial
3.  PCR Array	Enhance Your Stem Cell Biology Research Using An Advanced qPCR Technology: PCR Arrays
4.  Lentiviral-Based Pathway      Reporter System	Measure Signaling Pathway Activity in ANY Mammalian Cell
5.  miRNA qPCR Arrays & Assays	Simultaneous Detection of Genome-Wide or Pathway-Focused miRNA
6.  Pathway Reporter Assay	Cell-Based Assays for Rapidly Analyzing Pathway Signaling Activity
7.  RNA Interference	Knock Down Your Favorite Genes or Pathways with Ease & Confidence
8.  ELISArray	Analyze 12 Cytokines or Chemokines Simultaneously with ELISArrays
9.  Chromatin IP & Real-Time PCR	ChampionChIP Chromatin Immunoprecipitation & Real-Time PCR Technology Overview
10.  DNA Methylation PCR System	Profile the Methylation Status of Multiple Genes Simultaneously Without Bisulfite

Personalize a PCR Array for Your Genes of Interest Would you like a way to analyze the expression of genes that you're interested in? With Custom PCR Arrays, you can! Learn how to profile up to 384 different genes with PCR Array technology. Custom PCR Arrays from SABiosciences are optimized under a rigorous set of parameters, providing highly efficient, reproducible, and specific detection of your genes of interest. Free data analysis software is also included with your Custom PCR Arrays. A technology overview and protocol tutorial will be presented here. Duration: 45 minutes followed by Q&A session. Schedule: Tuesday, January 27, 2009 at 1:00 PM Eastern    Status: Available Reserve

PCR Array Data Analysis Tutorial With a live demonstration using actual PCR Array data, learn how easy it is to use our data analysis web portal to calculate fold-differences in gene expression from your raw real-time PCR threshold cycles. Duration: 45 minutes followed by Q&A session. Schedule: Thursday, January 8, 2009 at 1:00 PM Eastern    Status: Available Reserve Friday, January 16, 2009 at 1:00 PM Eastern    Status: Available Reserve Thursday, January 22, 2009 at 1:00 PM Eastern    Status: Available Reserve Wednesday, January 28, 2009 at 1:00 PM Eastern    Status: Available Reserve

Enhance Your Stem Cell Biology Research Using An Advanced qPCR Technology: PCR Arrays Understanding the systems biology of stem cells requires a systematic survey of their gene, protein, and pathway activities. PCR Arrays from SABiosciences can help! This 45-minute seminar will describe research on the differentiation program of adult mesenchymal stem cells into neurons or osteoblasts using RT² microRNA PCR Arrays and RT² Profiler PCR Arrays. The specific genes and microRNA sequences that are differentially up-or down-regulated during neurogenesis or osteogenesis will be presented. The results will highlight the power of PCR Arrays to help delineate the roles of signaling pathway interactions and microRNA in stem cell differentiation. Duration: 45 minutes followed by Q&A session. Schedule: Wednesday, January 7, 2009 at 1:00 PM Eastern    Status: Available Reserve Thursday, January 15, 2009 at 1:00 PM Eastern    Status: Available Reserve

Measure Signaling Pathway Activity in ANY Mammalian Cell Would you like to measure signaling pathway activity in your favorite cell? Now you can! Attend this webinar to learn about our new breakthrough cellular assay technology. Cignal Lenti Pathway Reporters are the newest addition to the Cignal Reporter cell-based assay system. The combined power of a lentiviral delivery system with our robust transcription factor reporter technology enables you to study signal pathways (NF-kB, Wnt, MAPK, hypoxia, Notch, and many more) in virtually any cell type. A technology overview, protocol tutorial, and application examples will be presented. Duration: 45 minutes followed by Q&A session. Schedule: Wednesday, January 21, 2009 at 1:00 PM Eastern    Status: Available Reserve

Simultaneous Detection of Genome-Wide or Pathway-Focused miRNA Your genes or pathways of interest are most likely regulated by micro RNA. How can you tell which miRNA sequences might be responsible? Lean how to apply a simple and accurate real-time PCR technique to identify miRNA expression patterns that correlate with your biological phenotypes of interest. See application examples for discovery experiments with most of the human miRNA genome, and cancer and development research using focused miRNA panels. Duration: 45 minutes followed by Q&A session. Schedule: Wednesday, January 14, 2009 at 1:00 PM Eastern    Status: Available Reserve

Cell-Based Assays for Rapidly Analyzing Pathway Signaling Activity Are you interested in studying the regulation of cell signaling pathways? Learn how to successfully apply convenient and robust reporter assays to your RNA interference, gene over-expression, protein, or small molecule studies. These assays are an excellent tool for studying pathway signaling activity in cells that are amenable to transfection. Cignal Pathway Reporter Assays are available for studying numerous pathways (Wnt, NF-kB, Notch, cAMP/PKA, TGFβ, and many more) relevant to cancer, immunology, and development. Duration: 45 minutes followed by Q&A session. Schedule: Tuesday, January 13, 2009 at 1:00 PM Eastern    Status: Available Reserve

Knock Down Your Favorite Genes or Pathways with Ease & Confidence Off-target effects and knockdown efficiency are two major concerns for gene function studies using RNA interference. Learn how our SureSilencing shRNA Plasmids and siRNA Arrays address these concerns with rigorous knockdown validation, multiple plasmid-based shRNA designs, and knocking down genes in the same pathway. Studies focused on cancer and immunology will be discussed to illustrate the applications of these gene knockdown products. Duration: 45 minutes followed by Q&A session. Schedule: Tuesday, January 6, 2009 at 1:00 PM Eastern    Status: Available Reserve Friday, January 23, 2009 at 1:00 PM Eastern    Status: Available Reserve

Analyze 12 Cytokines or Chemokines Simultaneously with ELISArrays We worked hard to make multi-analyte ELISA easy! Learn how to profile twelve cytokines or chemokines in six samples all at the same time with our new Multi-Analyte and Single Analyte ELISArray technology. The ELISArray kits are optimized assays for measuring human or mouse cytokine or chemokine expression in cell culture supernatants, serum or plasma. A technology overview, protocol tutorial, and application data will be presented. Duration: 45 minutes followed by Q&A session. Schedule: Thursday, January 29, 2009 at 1:00 PM Eastern    Status: Available Reserve

ChampionChIP Chromatin Immunoprecipitation & Real-Time PCR Technology Overview Interested in studying how specific transcription factors and histone modifications regulate the expression of genes? Learn how to measure in vivo protein and genomic DNA interactions with the proven and quantitative ChampionChIP System for real-time PCR analysis of chromatin immunoprecipitation (ChIP). In this 45-minute seminar, see how easy a ChIP experiment can really be with our One-Day ChIP preparation kit, how our search portal finds the right PCR primers for your transcription factor and/or gene of interest, and how the system can be applied to your research. Duration: 45 minutes followed by Q&A session. Schedule: Friday, January 30, 2009 at 1:00 PM Eastern    Status: Available Reserve

Profile the Methylation Status of Multiple Genes Simultaneously Without Bisulfite The DNA methylation profiles of promoter CpG islands are important epigenetic biomarkers to both cancer and development research. Traditional bisulfite-based methods are too tedious and time-consuming to effectively keep pace with the needed rate of biomarker discovery and validation. In this 45-minute seminar, learn about the new Methyl-Profiler DNA Methylation PCR Arrays, a faster, easier, and higher-throughput DNA methylation profiling method that combines the reliability of well-controlled restriction digestions with quantitative PCR detection. Duration: 45 minutes followed by Q&A session. Schedule: Friday, January 9, 2009 at 1:00 PM Eastern    Status: Available Reserve Tuesday, January 20, 2009 at 1:00 PM Eastern    Status: Available Reserve

Podcasts:

SABiosciences is now Podcasting! Download a pre-recorded seminar. Watch and listen on your iPod or on your computer on your own time - on the train or the bus, at home, during lunch, or while working in the lab.

How It Works:

• You don't even need an iPod or the latest versions of any media player!
• Just click the link for each topic.
• Go a web-based media player to view the recorded session.

Current Seminar Titles Available:

Focus	Title	Download
PCR Array	PCR Array Data Analysis Tutorial
PCR Array	How the PCR Arrays Work: Simple & Accurate Pathway Profiling
miRNA qPCR Arrays & Assays	Simultaneous Detection of Genome-Wide or Pathway-Focused miRNA
RNA Interference	Knock Down Your Favorite Genes or Pathways with Ease & Confidence
ELISArrays	Analyzing Multiple Cytokines or Chemokines Simultaneously with ELISA
Reporter Assay	Cell-Based Assays for Rapidly Analyzing Pathway Signaling Activity

PowerPoint Presentations

Download PDF versions of the PowerPoint presentations used for our previous or existing live on-line seminars and podcasts.

Current Seminar Titles Available:

Focus	Title	Download
PCR Array	How the PCR Arrays Work: Simple & Accurate Pathway Profiling
PCR Array	PCR Array Protocol Tutorial
miRNA qPCR Arrays & Assays	Simultaneous Detection of Genome-Wide or Pathway-Focused miRNA
RNA Interference	Knock Down Your Favorite Genes or Pathways with Ease & Confidence
ELISArrays	Analyzing Multiple Cytokines or Chemokines Simultaneously with ELISA
Reporter Assay	Cell-Based Assays for Rapidly Analyzing Pathway Signaling Activity
Lentiviral-Based Pathway Reporter System	Measure Signaling Pathway Activity in ANY Mammalian Cell
Cell-based Protein Phosphorylation Assay	Directly Measure Protein Phosphorylation without Cell Lysis