SABiosciences Seminars

SABiosciences is pleased to provide free, on-line educational resources covering relevant tools and techniques for gene and protein expression analysis, and cell-based gene function analysis. These presentations can also help you get started with our pathway-focused and genome-wide technologies. Choose from:

Web Seminars:

SABiosciences offers free, on-line seminars covering relevant tools and techniques for gene and protein expression analysis, and cell-based gene function analysis. Attendance is free, but we do ask you to register with us. These educational seminars require just Internet access (we use the WebEx conferencing service) and a TOLL-FREE phone call to hear the presentation and ask questions. After registering, you will receive confirmation e-mail, then a reminder e-mail on the morning of the seminar. We rotate the seminar topics and update the schedule, so please check back periodically for new topics and new dates.

Current Seminar Titles Available:

Focus	Title
1.  PCR Array	PCR Array Data Analysis Tutorial
2.  PCR Array	PCR Arrays: The Real Pioneer in Real-Time PCR Analysis of Biological Pathways
3.  Lentiviral-Based Pathway      Reporter System	Measure Signaling Pathway Activity in ANY Mammalian Cell
4.  MicroRNA PCR Array	Simultaneous Detection of Genome-Wide or Pathway-Focused miRNA
5.  Pathway Reporter Assay	Cell-Based Assays for Rapidly Analyzing Pathway Signaling Activity
6.  RNA Interference	Knock Down Your Favorite Genes or Pathways with Ease & Confidence
7.  ELISArray	Analyze 12 Cytokines or Chemokines Simultaneously with ELISArrays
8.  Chromatin IP & Real-Time PCR	ChampionChIP Chromatin Immunoprecipitation & Real-Time PCR Technology Overview
9.  DNA Methylation PCR System	Profile the Methylation Status of Multiple Genes Simultaneously Without Bisulfite
10.  FFPE & Small RNA Sample PCR Systems	FFPE and Small Sample Gene Expression Profiling with PCR Arrays

PCR Array Data Analysis Tutorial With a live demonstration using actual PCR Array data, learn how easy it is to use our data analysis web portal to calculate fold-differences in gene expression from your raw real-time PCR threshold cycles. Duration: 45 minutes followed by Q&A session. Schedule: Tuesday, July 7, 2009 at 1:00 PM Eastern    Status: Available Reserve Thursday, July 16, 2009 at 1:00 PM Eastern    Status: Available Reserve Wednesday, July 22, 2009 at 1:00 PM Eastern    Status: Available Reserve Friday, July 31, 2009 at 1:00 PM Eastern    Status: Available Reserve

PCR Arrays: The Real Pioneer in Real-Time PCR Analysis of Biological Pathways In this 45-minute seminar, see how the PCR Array has truly led the way toward the simple and accurate real-time PCR analysis of relevant biological pathways and diseases. Learn about the stringent performance built into the technology to insure its sensitivity, specificity, reproducibility, and reliability. Find out how researchers like you have applied the PCR Arrays to their cancer, immunology, and toxicology research. Duration: 45 minutes followed by Q&A session. Schedule: Friday, July 10, 2009 at 1:00 PM Eastern    Status: Available Reserve Wednesday, July 15, 2009 at 1:00 PM Eastern    Status: Available Reserve Thursday, July 23, 2009 at 1:00 PM Eastern    Status: Available Reserve Tuesday, July 28, 2009 at 1:00 PM Eastern    Status: Available Reserve

Measure Signaling Pathway Activity in ANY Mammalian Cell Would you like to measure signaling pathway activity in your favorite cell? Now you can! Attend this webinar to learn about our new breakthrough cellular assay technology. Cignal Lenti Pathway Reporters are the newest addition to the Cignal Reporter cell-based assay system. The combined power of a lentiviral delivery system with our robust transcription factor reporter technology enables you to study signal pathways (NF-kB, Wnt, MAPK, hypoxia, Notch, and many more) in virtually any cell type. A technology overview, protocol tutorial, and application examples will be presented. Duration: 45 minutes followed by Q&A session. Schedule: Thursday, July 30, 2009 at 1:00 PM Eastern    Status: Available Reserve

Simultaneous Detection of Genome-Wide or Pathway-Focused miRNA Your genes or pathways of interest are most likely regulated by micro RNA. How can you tell which miRNA sequences might be responsible? Lean how to apply a simple and accurate real-time PCR technique to identify miRNA expression patterns that correlate with your biological phenotypes of interest. See application examples for discovery experiments with most of the human miRNA genome, and cancer and development research using focused miRNA panels. Duration: 45 minutes followed by Q&A session. Schedule: Friday, July 24, 2009 at 1:00 PM Eastern    Status: Available Reserve

Cell-Based Assays for Rapidly Analyzing Pathway Signaling Activity Are you interested in studying the regulation of cell signaling pathways? Learn how to successfully apply convenient and robust reporter assays to your RNA interference, gene over-expression, protein, or small molecule studies. These assays are an excellent tool for studying pathway signaling activity in cells that are amenable to transfection. Cignal Pathway Reporter Assays are available for studying numerous pathways (Wnt, NF-kB, Notch, cAMP/PKA, TGFβ, and many more) relevant to cancer, immunology, and development. Duration: 45 minutes followed by Q&A session. Schedule: Thursday, July 9, 2009 at 1:00 PM Eastern    Status: Available Reserve

Knock Down Your Favorite Genes or Pathways with Ease & Confidence Off-target effects and knockdown efficiency are two major concerns for gene function studies using RNA interference. Learn how our SureSilencing shRNA Plasmids and siRNA Arrays address these concerns with rigorous knockdown validation, multiple plasmid-based shRNA designs, and knocking down genes in the same pathway. Studies focused on cancer and immunology will be discussed to illustrate the applications of these gene knockdown products. Duration: 45 minutes followed by Q&A session. Schedule: Friday, July 17, 2009 at 1:00 PM Eastern    Status: Available Reserve

Analyze 12 Cytokines or Chemokines Simultaneously with ELISArrays We worked hard to make multi-analyte ELISA easy! Learn how to profile twelve cytokines or chemokines in six samples all at the same time with our new Multi-Analyte and Single Analyte ELISArray technology. The ELISArray kits are optimized assays for measuring human or mouse cytokine or chemokine expression in cell culture supernatants, serum or plasma. A technology overview, protocol tutorial, and application data will be presented. Duration: 45 minutes followed by Q&A session. Schedule: Tuesday, July 14, 2009 at 1:00 PM Eastern    Status: Available Reserve

ChampionChIP Chromatin Immunoprecipitation & Real-Time PCR Technology Overview Interested in studying how specific transcription factors and histone modifications regulate the expression of genes? Learn how to measure in vivo protein and genomic DNA interactions with the proven and quantitative ChampionChIP System for real-time PCR analysis of chromatin immunoprecipitation (ChIP). In this 45-minute seminar, see how easy a ChIP experiment can really be with our One-Day ChIP preparation kit, how our search portal finds the right PCR primers for your transcription factor and/or gene of interest, and how the system can be applied to your research. Duration: 45 minutes followed by Q&A session. Schedule: Wednesday, July 29, 2009 at 1:00 PM Eastern    Status: Available Reserve

Profile the Methylation Status of Multiple Genes Simultaneously Without Bisulfite The DNA methylation profiles of promoter CpG islands are important epigenetic biomarkers to both cancer and development research. Traditional bisulfite-based methods are too tedious and time-consuming to effectively keep pace with the needed rate of biomarker discovery and validation. In this 45-minute seminar, learn about the new Methyl-Profiler DNA Methylation PCR Arrays, a faster, easier, and higher-throughput DNA methylation profiling method that combines the reliability of well-controlled restriction digestions with quantitative PCR detection. Duration: 45 minutes followed by Q&A session. Schedule: Wednesday, July 8, 2009 at 1:00 PM Eastern    Status: Available Reserve

FFPE and Small Sample Gene Expression Profiling with PCR Arrays Learn how to analyze gene expression profiles of RNA from FFPE samples and samples containing as little as 1 nanogram of recoverable RNA (ex. Fine Needle Biopsies (FNB), Laser Capture Microdissection (LCM), Fluorescent Activated Cell Sorting (FACS), and Stem Cells).  SABiosciences' FFPE Extraction Kit and FFPE PreAMP/Nano PreAMP technology enables the use of RT2 Profiler PCR Array-based gene expression analysis on these samples.  Log on to this webinar to learn more about this technology, the protocol, and view application examples. Duration: 45 minutes followed by Q&A session. Schedule: Tuesday, July 21, 2009 at 1:00 PM Eastern    Status: Available Reserve

Podcasts:

SABiosciences is now Podcasting! Download a pre-recorded seminar. Watch and listen on your iPod or on your computer on your own time - on the train or the bus, at home, during lunch, or while working in the lab.

How It Works:

• You don't even need an iPod or the latest versions of any media player!
• Just click the link for each topic.
• Go a web-based media player to view the recorded session.

Current Seminar Titles Available:

Focus	Title	Download
PCR Array	PCR Array Data Analysis Tutorial
PCR Array	The Real Pioneer in Real-Time PCR Analysis of Biological Pathways
Small Sample Analysis	FFPE and Small Sample Gene Expression Profiling with PCR Arrays
miRNA qPCR Arrays & Assays	Simultaneous Detection of Genome-Wide or Pathway-Focused miRNA
RNA Interference	Knock Down Your Favorite Genes or Pathways with Ease & Confidence
ELISArrays	Analyzing Multiple Cytokines or Chemokines Simultaneously with ELISA
Reporter Assay	Cell-Based Assays for Rapidly Analyzing Pathway Signaling Activity
DNA Methylation	Profile the Methylation Status of Multiple Genes Simultaneously Without Bisulfite
Chromatin Immunoprecipitation	ChampionChIP Chromatin Immunoprecipitation & Real-Time PCR Technology Overview

PowerPoint Presentations

Download PDF versions of the PowerPoint presentations used for our previous or existing live on-line seminars and podcasts.

Current Seminar Titles Available:

Focus	Title	Download
PCR Array	How the PCR Arrays Work: Simple & Accurate Pathway Profiling
PCR Array	PCR Array Protocol Tutorial
Small Sample Analysis	FFPE and Small Sample Gene Expression Profiling with PCR Arrays
Custom PCR Arrays	Complete Real-Time PCR Gene Expression Analysis Solution for Your Genes of Interest
miRNA qPCR Arrays & Assays	Simultaneous Detection of Genome-Wide or Pathway-Focused miRNA
RNA Interference	Knock Down Your Favorite Genes or Pathways with Ease & Confidence
ELISArrays	Analyzing Multiple Cytokines or Chemokines Simultaneously with ELISA
Reporter Assay	Cell-Based Assays for Rapidly Analyzing Pathway Signaling Activity
Lentiviral-Based Pathway Reporter System	Measure Signaling Pathway Activity in ANY Mammalian Cell
Cell-based Protein Phosphorylation Assay	Directly Measure Protein Phosphorylation without Cell Lysis
DNA Methylation PCR System	Profile the Methylation Status of Multiple Genes Simultaneously Without Bisulfite
ChIP-qPCR System	Quick, High Throughput Chromatin Immunoprecipitation Assay System