QIAGEN Website    Quick Order    Online Seminar    Contact    My Account
Bookmark and Share Home  >  Resources  >  Online Seminars

For the most updated product information, visit GeneGlobe on QIAGEN website

Online Seminars

SABiosciences is pleased to provide free, on-line educational resources covering relevant tools and techniques for gene and protein expression analysis, and cell-based gene function analysis. We offer live webinars, prerecorded and PDF formatted presentations.

See the instructions for the Web Seminars

February 2016
Monday
Tuesday
Wednesday
Thursday
Friday

1

Onestep ahead RT-PCR

2

QPCR Introduction

3

miRNA overview

4

Host–pathogen interactions

5

8

Profiling miRNA from single cells

9

Coding & noncoding RNA analysis using qPCR

10

Functional analysis of miRNAs

11

Enrich microbial DNA with our protocol

NGS in mutation identification of cell-free circulating tumor DNA

12

15

long noncoding RNA (lncRNA)

gDNA collection, storage, and purification

16

PCR Array Data Analysis Tutorial

Single cell analysis: sample to insight

17

Introduction to RNAseq

Practical hints for successful PCR

18

Microbiome: From identification to characterization

Clinical sequencing for meningiomas

19

long noncoding RNA (lncRNA)

22

Quality control of DNA - tips & tricks

23

Service for biomarker research

Analysis of Single-Cell Sequencing Data

24

RNAseq targeted panels

Controls and novel solutions for real-time qPCR

25

long noncoding RNA (lncRNA)

26

Target NGS for cancer research

29

gDNA isolation from solid tissue samples

Current Seminar Titles Available:

Focus Title
1.  PCR ArrayPCR Array Data Analysis Tutorial
2.  miRNAsFunctional analysis of miRNAs
3.  Microbial PCR ArraysMicrobiome: From identification to characterization
4.  lncRNANew advances in long noncoding RNA (lncRNA) research
5.  Next-generation sequencingNext-Generation Sequencing — Targeted Enrichment Technology in Cancer Research
6.  Single cell analysisSingle cell analysis - from sample to insight
7.  Service CoreAccelerate your discovery with QIAGEN service solutions for biomarker research
8.  Multiplex PCRPractical hints and new solutions for successful real-time PCR studies
9.  PCR ArraysAdvanced Real-Time PCR Array Technology – Coding & Noncoding RNA Expression Analysis
10.  Single cell analysisAnalysis of Single-Cell Sequencing Data by CLC / Ingenuity
11.  DNA sample isolationTips and Tricks for Rapid Isolation of Genomic DNA from Solid Tissue Samples
12.  DNA sample isolationFundamental Concepts and Special Considerations in gDNA Isolation for Better Insight
13.  DNA sample isolationChallenges of FFPE Sample Materials – Where Does Variation in Quantity of Purified DNA Come From?
14.  Next-generation sequencingUtilization of Next-Generation Sequencing to Identify Clinically-Relevant Mutations in Cell-Free Circulating Tumor DNA
15.  Next-generation sequencingDeveloping a rapid clinical sequencing system to classify meningioma
16.  Microbiome researchMicrobiome: isolate and enrich microbial DNA with our new protocol
17.  RNAseq technologyDigital RNASeq: Technology introduction
18.  RNAseq technologyDigital RNAseq for gene expression profiling: Workflow and applications
19.  RNAseq technologyMolecular insight into gene expression using Digital RNAseq: Data analysis tutorial
20.  QuantiNova PCR kit The importance of controls and novel solutions for successful real-time qPCR

PCR Array Data Analysis Tutorial

With a live demonstration using actual PCR array data, learn how easy it is to use our data analysis Web portal to calculate fold-differences in gene expression from your raw real-time PCR threshold cycles.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, February 16, 2016 at 9:30 AM Eastern    Status: Available Reserve

Functional analysis of miRNAs

Understanding the function and targets of miRNA is important, but challenging research. Since one miRNA can potentially target several genes, finding and validating a specific miRNA-mRNA interaction often involves multiple molecular techniques. In this webinar we will highlight the use of miRNA mimics, inhibitors and target protectors to increase, decrease and adjust the cellular concentration of miRNA and disrupt specific miRNA-mRNA interactions. We will also present a ready-to-use screening tool for identifying miRNA targets in your samples. Lastly, we will show how miRNA expression data can be used to predict mRNA targets and how qPCR can be used to validate predicted interactions.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, February 10, 2016 at 9:30 AM Eastern    Status: Available Reserve

Microbiome: From identification to characterization

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, February 18, 2016 at 9:30 AM Eastern    Status: Available Reserve

New advances in long noncoding RNA (lncRNA) research

"Recent improvements in RNA-sequencing have led to the discovery of thousands of RNAs that do not contain any open reading frames, termed “long noncoding RNA (lncRNA)”. lncRNA expression is thought to be important for the regulation of gene (mRNA) expression through several different mechanisms, some involving competition with transcription and others involving the recruitment of specific epigenetic factors to promoter regions. lncRNA may also indirectly affect gene expression by interacting with miRNA and other cellular factors. In this webinar, we will review what is known about lncRNA and use application examples in cancer research to illustrate how expression profiling is being used to identify lncRNA biomarkers from cells, tissues, blood, and exosomes. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, February 15, 2016 at 9:30 AM Eastern    Status: Available Reserve
Friday, February 19, 2016 at 11:00 AM Eastern    Status: Available Reserve
Thursday, February 25, 2016 at 1:00 PM Eastern    Status: Available Reserve

Next-Generation Sequencing — Targeted Enrichment Technology in Cancer Research

Genetic variance analysis plays a critical role cancer diagnosis, prognosis and treatment of cancer. Low sample volume and poor DNA quality are nagging issues with cancer samples making the results unreliable and expensive. This webinar discusses the most biologically efficient, cost-effective method for successful NGS. The GeneRead DNA QuantiMIZE Kits enable determination of the optimum conditions for targeted enrichment of DNA isolated from biological samples, while the GeneRead DNAseq Panels V2 allow you to quickly and reliably deep sequence your genes of interest. Applications in translational and clinical research will be highlighted.

Duration: 45 minutes followed by Q&A session.

Schedule:

Friday, February 26, 2016 at 11:00 AM Eastern    Status: Available Reserve

Single cell analysis - from sample to insight

What can you do from a single cell? Actually, a lot! Beginning with the genome, you can discover new biomarkers by identifying new genetic variances and their association with specific diseases, including cancers. Moving on to RNA, the recent advances in RNA sequencing technology have made single-cell transcriptomics a possibility. Along with these possibilities come challenges that start from the moment you get the sample to the final step of gaining insights into the cell. This webinar will provide an overview on the multiple steps involved as you move from sample acquisition to analysis and data interpretation in different sample types.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, February 16, 2016 at 1:00 PM Eastern    Status: Available Reserve

Accelerate your discovery with QIAGEN service solutions for biomarker research

Development of preclinical biomarkers can have several pitfalls, often requiring a large investment in instruments to process samples, time to develop assays, and specialties in data analysis and biomarker algorithm design. QIAGEN’s life sciences service core can provide researchers with the tools, knowledge and specialties to help develop the next generation of biomarkers for cancer, toxicology and inflammation research. This seminar will highlight QIAGEN’s service capabilities in sample isolation, microarray and NGS-sequencing, qPCR panel, and custom assay development and bioinformatics as we look at the identification of potential biomarkers and gene signatures. We will discuss the applications of QIAGEN Service Core in microRNA discovery for toxicology markers in serum and plasma and in identification of RNA signatures for tumor stratification. Join us and learn how you can accelerate your research with QIAGEN service solutions.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, February 23, 2016 at 9:30 AM Eastern    Status: Available Reserve

Practical hints and new solutions for successful real-time PCR studies

In this webinar we will cover the following topics which are critical steps for efficient and precise gene expression studies using real-time PCR technology: - Effect of RNA integrity on real-time PCR results – tips to achieve a true RNA profiling suitable for real-time PCR studies - Improved methods for cDNA synthesis, optimized for real-time PCR - Real-time PCR analysis • Real-time PCR essentials and background information on different quantification strategies • SYBR Green real-time PCR – factors influencing specificity • Introduction to probe technology • New, fast and efficient real-time PCR solutions

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, February 17, 2016 at 9:30 AM Eastern    Status: Available Reserve

Advanced Real-Time PCR Array Technology – Coding & Noncoding RNA Expression Analysis

This webinar presents a simple and accurate real-time PCR system for relevant biological pathway- & disease-focused mRNA and long non-coding RNA (lncRNA) expression profiling. Learn about the stringent performance built into the technology to insure its sensitivity, specificity, reproducibility, and reliability. Application examples will be presented.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, February 9, 2016 at 9:30 AM Eastern    Status: Available Reserve

Analysis of Single-Cell Sequencing Data by CLC / Ingenuity

"Single-cell analysis is useful to study genetic heterogeneity between individual cells and can help in result interpretation by looking at the average behavior of a large number of cells. Applications include circulating tumor cells, cells from small biopsies and cells from in vitro fertilized embryos. In this webinar, we will show how single-cell next-generation sequencing data can be analyzed and what challenges needs to be overcome. One of the examples we will use is single cell data from two colorectal cancer cell lines. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, February 23, 2016 at 1:00 PM Eastern    Status: Available Reserve

Tips and Tricks for Rapid Isolation of Genomic DNA from Solid Tissue Samples

Obtaining reliable results from genotyping or sequencing experiments using solid tissue is not always easy. In order to have a successful experiment, you must start with sufficient amounts of high-quality DNA. An inefficient lysis results in poor yields, low DNA integrity and consequently, inferior quality results, thereby limiting the insights you can attain from your sample. In this webinar, we will talk about solutions to overcome the challenges of lysis of fibrous or hard tissue so that you can achieve the insights you value.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, February 29, 2016 at 1:00 PM Eastern    Status: Available Reserve

Fundamental Concepts and Special Considerations in gDNA Isolation for Better Insight

"How are your gDNA yields? Some sample types present special challenges in DNA purification and analysis. This webinar provides tips for a whole range of sample types that require special consideration. We will cover the following topics in detail: • The basic methods and challenges of gDNA purification • Working with DNA: good laboratory practice • Special considerations for challenging sample types • Performing DNA quality checks"

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, February 15, 2016 at 1:00 PM Eastern    Status: Available Reserve

Challenges of FFPE Sample Materials – Where Does Variation in Quantity of Purified DNA Come From?

In this live webinar, we will discuss variability in quantity and purity of DNA purified from FFPE samples manually or with automated procedures, assessed by different quantification and quality control methods. We've carried out a comprehensive analysis, so register today and see the results!

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, February 22, 2016 at 1:00 PM Eastern    Status: Available Reserve

Utilization of Next-Generation Sequencing to Identify Clinically-Relevant Mutations in Cell-Free Circulating Tumor DNA

Pancreatic cancer is a uniquely lethal malignancy characterized by frequent mutations in KRAS, CDKN2A, SMAD4, TP53 and many others. We have shown that KRAS mutation can be detected in cell-free, circulating tumor DNA (ctDNA) isolated from the plasma in a subset of patients and is associated with poor prognosis. The ability to simultaneously detect multiple pancreatic cancer-specific mutations in ctDNA would open a new avenue for detection of clinically-relevant mutations. In this study, we performed ultra-deep sequencing of ctDNA from advanced pancreatic cancer patients prior to treatment with Gemcitabine and Erlotinib following target enrichment. Somatic, non-synonymous variants were identified in 29 different genes at allele frequencies typically <0.5%. Updated results of ultra-deep NGS analysis will be presented.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, February 11, 2016 at 11:00 AM Eastern    Status: Available Reserve

Developing a rapid clinical sequencing system to classify meningioma

Meningioma’s display a broad spectrum of clinical, histological and cytogenetic features even within the same WHO grade often posing a challenge for classification and prognostic stratification. In this webinar, we will describe our experience of using targeted amplicon sequencing to develop rapid clinical sequencing system to identify and confirm the meningioma genotype in just 7 days. In addition the details of the three meningioma categories and the genes involved will be discussed.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, February 18, 2016 at 6:00 PM Eastern    Status: Available Reserve

Microbiome: isolate and enrich microbial DNA with our new protocol

Human microbiome projects have delivered our first glimpse into microbial communities that reside in and on our bodies. However, distinguishing microbial DNA from host DNA remains one of the most problematic challenges facing researchers today. We have developed an easy-to-use workflow that allows selective isolation of microbial DNA from samples that are intrinsically rich in host DNA. This protocol includes steps for efficient depletion of host DNA while providing optimized conditions specific for bacterial lysis. This workflow is also specific for the identification of live bacteria, avoiding false results due to nucleic acids from dead bacteria. Enriched microbial DNA can be directly used in other molecular methods such as whole genome sequencing, qPCR and microarray assays.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, February 11, 2016 at 9:30 AM Eastern    Status: Available Reserve

Digital RNASeq: Technology introduction

QIAseq RNA is a revolutionary turnkey solution for digital gene expression analysis by NGS. From 10 genes to 1000, from one sample to 100, QIAseq RNA delivers precise results on both ION and Illumina sequencing platforms. The data from QIAseq RNA is directly comparable to expression analysis derived from whole transcriptome sequencing or by qRTPCR, only better, cheaper, faster and more flexible. This webinar will describe the principles of digital expression analysis by NGS, and review the features and benefits of the QIAseq system, options available and the integrated data analysis package.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, February 17, 2016 at 1:00 PM Eastern    Status: Available Reserve

Digital RNAseq for gene expression profiling: Workflow and applications

Traditional RNA sequencing (RNA-Seq) is a powerful tool for expression profiling, but is hindered by PCR amplification bias and inaccuracy at low expressing genes. QIAseq RNA is a flexible and precise tool developed for mitigating these complications, allowing digital gene expression analysis. This in-depth webinar will cover sample requirements, experimental design, NGS platform-specific challenges and workflow for gene enrichment, library prep and sequencing. The applications of QIASeq RNA Panels in cancer research, stem cell differentiation and elucidating the effects small molecules on signaling pathways will be highlighted.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, February 24, 2016 at 1:00 PM Eastern    Status: Available Reserve

Molecular insight into gene expression using Digital RNAseq: Data analysis tutorial

Gene expression profiling is the key to understanding biological pathways and complex cellular systems. In this webinar, we will discuss the challenges of targeted RNA-seq data analysis and present the solutions provided by the QIAGEN automated online data analysis tools. Using raw sequencing data from targeted sequencing, the output of the QIAseq primary data analysis tool and the options in QIAseq secondary analysis, such as normalization strategies, will be described. The use of Ingenuity Pathway Analysis (IPA) to unlock the molecular insights buried in experimental data by quickly identifying relationships, mechanisms, functions, and pathways of relevance will be shown with an example.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, March 2, 2016 at 1:00 PM Eastern    Status: Available Reserve

The importance of controls and novel solutions for successful real-time qPCR

"The increasing demand for streamlined, monitored, and ultrafast qPCR procedures requires high-performance, real-time quantitative RT and PCR chemistries. Particularly procedures utilizing generic kits for gene expression analysis should include in-process safety measures to avoid variables and control accuracy of procedures and results. This webinar presents innovative solutions for one-step and two-step RT-PCR that significantly enhance performance and reliability in qRT-PCR. The new QuantiNova kit family offers a combination of various integrated safety features to remove variables and prevent artifacts. Internal Control RNA, removal of genomic DNA, room temperature set up capability for RT-PCR, and a built-in visual pipetting control verify accurate procedures, ensuring reliable gene expression profiling. This webinar explains the principles of the technologies and shows data demonstrating performance in qRT-PCR. Find out how you can verify accurate performance in qRT-PCR and improve your results."

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, February 24, 2016 at 9:30 AM Eastern    Status: Available Reserve

Note: Viewers will be asked to register before viewing the previously recorded webinars.