See the instructions for the Web Seminars

May 2013
Monday	Tuesday	Wednesday	Thursday	Friday
		1 Epigenetic Technologies for Biomedical Research	2 miRNA overview QPCR Introduction	3 NGS: an introduction to technology and applications
6 RNAi Screen Design & Optimzation Cell Death Research Solutions	7 QPCR Introduction Oxidative Stress Research Solutions	8 ChIP and Real-Time PCR Technology Overview	9 miRNAs expression and quantitation PCR Arrays for Pathway Analysis	10 NGS for cancer research
13	14 PCR Arrays for Pathway Analysis Insulin resistance	15 Profiling DNA Methylation with PCR System	16 miRNAs in Serum PCR Array Data Analysis Tutorial	17 NGS data anlaysis
20 RNAi Gene Knockdown with shRNA Metabolism and cancer	21 PCR Array Data Analysis Tutorial Copy number: variation & alteration analysis strategies	22 Functional analysis of miRNAs	23 miRNA Data Analysis Biologically Interpret Your Gene Expression Analysis Results	24 Functional analysis of genes, biologics & small molecules
27	28 Biologically Interpret Your Gene Expression Analysis Results	29 Molecular Mechanisms of Neurodegeneration Stem cell overview	30 Oncogenomics & cancer mutation PCR Arrays for Pathway Analysis	31 Cancer and Inflammation

Current Seminar Titles Available:

Focus	Title
1.  PCR Array	PCR Array Data Analysis Tutorial
2.  PCR Array	PCR Arrays: The Real Pioneer in Real-Time PCR Analysis of Biological Pathways
3.  Epigenetic Products	New Epigenetic Technologies for Biomedical Research: microRNA, Chromatin IP, and DNA Methylation PCR Arrays
4.  Somatic Mutation	Cancer Mutation Analysis Made Easy – qBiomarker Somatic Mutation PCR Arrays
5.  miRNA Data Analysis	miRNA Data Analysis
6.  QPCR Introduction	Introduction to Real Time PCR (Q-PCR/qPCR/qrt-PCR)
7.  miRNAs	The miRNA revolution: An introduction to microRNA biogenesis, function & analysis
8.  miRNAs	miRNA Quantitation: From experimental design through data analysis
9.  miRNAs	Profiling miRNA expression in cells, FFPE samples, and serum: on the road to biomarker development.
10.  Chromatin Regulation	Chromatin-based regulation of gene expression
11.  Copy number analysis	Copy Number: Variation and Alteration Analysis Strategies
12.  Cancer and Inflammation	Cancer and Inflammation: How Strong is the Link?
13.  PCR Array Analysis	Biologically Interpret Your Gene Expression Analysis Results
14.  Inflammation and TLR	Toll-like Receptors in Inflammation
15.  Neurodegeneration Mechanisms	Molecular Mechanisms of Neurodegeneration
16.  miRNAs	Functional analysis of miRNAs
17.  Next-generation sequencing	Next-generation sequencing, an introduction to technology and applications
18.  Next-generation sequencing	Next-generation sequencing for cancer research
19.  Next-generation sequencing	Next-generation sequencing data analysis for genetic profiling
20.  Insulin resistance	Insulin resistance regulation and energy metabolism
21.  Stem cell	Stem cell: overview and key stem cell signaling pathways
22.  PCR Arrays	Biology-Focused Research: How QIAGEN Develops Scientifically Relevant Array-Based Products

PCR Array Data Analysis Tutorial With a live demonstration using actual PCR array data, learn how easy it is to use our data analysis Web portal to calculate fold-differences in gene expression from your raw real-time PCR threshold cycles. Duration: 45 minutes followed by Q&A session. Schedule: Monday, June 17, 2013 at 1:00 PM Eastern    Status: Available Reserve Thursday, June 27, 2013 at 9:30 AM Eastern    Status: Available Reserve

PCR Arrays: The Real Pioneer in Real-Time PCR Analysis of Biological Pathways In this 45-minute Webinar, see how the PCR array has led the way toward simple and accurate real-time PCR analysis of relevant biological pathways and diseases. Learn about the stringent performance built into the technology to ensure its sensitivity, specificity, reproducibility, and reliability. Find out how researchers like you have applied PCR arrays to their cancer, immunology, and toxicology research. Duration: 45 minutes followed by Q&A session. Schedule: Thursday, May 30, 2013 at 11:00 PM Eastern    Status: Available Reserve Monday, June 10, 2013 at 1:00 PM Eastern    Status: Available Reserve Thursday, June 20, 2013 at 9:30 AM Eastern    Status: Available Reserve

New Epigenetic Technologies for Biomedical Research: microRNA, Chromatin IP, and DNA Methylation PCR Arrays Epigenetics has grabbed the attention of many researchers by providing new insights into cell differentiation and oncogenesis. These rapidly expanding studies examine how heritable factors not coded within genomic DNA sequences regulate gene expression. Such factors include DNA methylation, chromatin remodeling due to histone modification, and even, in some cases, microRNA. The experimental methods designed to discover and correlate these factors with biological phenotypes have evolved. However, many still suffer from poor sensitivity and reliability, low-throughput, and unnecessary complexity. This Webinar introduces advanced yet easy-to-use real-time PCR array technologies that analyze microRNA as well as mRNA expression profiles, CpG island DNA methylation, and chromatin immunoprecipitation fractions. Find out how easily real-time PCR-based epigenetics experiments can be performed in cancer and stem cell research to better understand the molecular epigenetic mechanisms regulating key genes of interest. Duration: 45 minutes followed by Q&A session. Schedule: Wednesday, June 5, 2013 at 1:00 PM Eastern    Status: Available Reserve

Cancer Mutation Analysis Made Easy – qBiomarker Somatic Mutation PCR Arrays How can you quickly and accurately characterize somatic mutations associated with cancer signaling pathways? Come join the Webinar to learn about the newest real-time, PCR-based product: qBiomarker Somatic Mutation PCR Arrays. Somatic mutations occur as single or multiple mutations within a gene or a panel of genes associated with tumorigenesis, such as genes in the EGFR, ErbB2, FLT3, and PDGF pathways. Responses to treatment regimens or biological phenotypes may differ based on a cells somatic mutation profile. Each qBiomarker Somatic Mutation PCR Array contains a panel of 5' nuclease assays for a stringently selected set of pathway focused somatic mutations, gene copy number controls, and PCR quality controls. Learn how you can profile the somatic mutation status in your samples with a simple 2-step protocol with the pathway-focused qBiomarker Somatic Mutation PCR Arrays. Duration: 45 minutes followed by Q&A session. Schedule: Thursday, May 30, 2013 at 1:00 PM Eastern    Status: Available Reserve Friday, June 21, 2013 at 1:00 PM Eastern    Status: Available Reserve

miRNA Data Analysis miRNAs are small functional RNAs which regulate gene expression post-transcriptionally. The RT2 miRNA PCR Array System is a sensitive and reliable technology for detection of mature miRNAs in any laboratory with access to a qPCR instrument. In this Webinar, we will discuss the challenges of miRNA data analysis and solutions that the RT2 miRNA PCR Arrays provide for researchers interested in identifying miRNA from cells, tissues, and FFPE samples. Using data generated with the RT2 miRNA PCR Arrays, we will demonstrate how our Web-based data analysis portal can be used to calculate fold-change values from raw Ct values. Learn how easy it is to identify miRNAs that may be important in your favorite biological pathway or disease. Duration: 45 minutes followed by Q&A session. Schedule: Thursday, June 27, 2013 at 1:00 PM Eastern    Status: Available Reserve

Introduction to Real Time PCR (Q-PCR/qPCR/qrt-PCR) Real-time polymerase chain reaction (qPCR) is the most sensitive and reliable method for the detection and quantification of nucleic acids. This 45-minute webinar introduces the concepts of real-time PCR and how to conduct a real-time PCR assay. The topics that will be covered include an overview of real-time PCR chemistries, protocols, quantification methods, real-time PCR applications, and factors for success. This webinar is for both beginners that are new to real-time PCR as well as experienced users. Duration: 45 minutes followed by Q&A session. Schedule: Monday, June 3, 2013 at 1:00 PM Eastern    Status: Available Reserve Thursday, June 6, 2013 at 9:30 AM Eastern    Status: Available Reserve

The miRNA revolution: An introduction to microRNA biogenesis, function & analysis miRNA plays a critical role in many biological processes such as differentiation and development, cell signaling, response to infection, and other challenges. Recent studies have shown overwhelming evidence that aberration in miRNA expression is causal or an indicator of many disease processes including cancer. Currently there is a race to find and develop miRNAs as biomarkers and therapeutics. To win the race, it is important to have a strong understanding of miRNA and the challenges of miRNA research. The webinar will cover the biology of miRNA, the key challenges associated with miRNA research, and the latest advances in miRNA research technology. Duration: 45 minutes followed by Q&A session. Schedule: Thursday, June 6, 2013 at 1:00 PM Eastern    Status: Available Reserve

miRNA Quantitation: From experimental design through data analysis miRNAs play a critical role in many biological processes including neuronal cell fate, hematology, apoptosis and developmental biology. Increasing evidence has shown that aberrations in microRNA regulation are associated with many disease processes. This webinar will discuss the challenges associated with miRNA quantification by real-time PCR, and the latest strategies for miRNA quantification and profiling. The critical parameters of profiling miRNA gene expression will also be presented. Come and learn how you can apply these tools in your miRNA research! Duration: 45 minutes followed by Q&A session. Schedule: Thursday, June 13, 2013 at 1:00 PM Eastern    Status: Available Reserve

Profiling miRNA expression in cells, FFPE samples, and serum: on the road to biomarker development. The holy grail of disease diagnosis is to use minimally invasive patient sampling to detect and analyze a stable biomarker with characteristics that are strongly prognostic of a particular disease or condition. Recent studies show overwhelming evidence that miRNAs are a normal component of the extracellular compartment of the blood, and are surprisingly stable in this state. How they get there, or what their normal role might be, remains under intensive investigation. However, multiple reports profiling circulating miRNAs from people with and without disease suggest that particular miRNAs may be ideal biomarkers. This webinar will cover the latest advances in miRNA research tools, as well as strategies and challenges for profiling miRNAs in cells, FFPE tissue, and serum samples.   Duration: 45 minutes followed by Q&A session. Schedule: Thursday, June 20, 2013 at 1:00 PM Eastern    Status: Available Reserve

Chromatin-based regulation of gene expression We know that gene expression requires the transcription of DNA sequences into RNA molecules which become translated into the proteins that facilitate biological pathways. But first, transcription factors, RNA polymerase, and other proteins must all be able to bind genomic DNA in the context of chromatin, a complex of DNA with structural DNA-binding proteins known as histones. Post-translational modifications of histones define whether local chromatin regions are in an open or a closed state thereby controlling other proteins’ accessibility to specific genes. Fully understanding the biology behind a phenotype requires analyzing not only the expression of the responsible genes, but also the chromatin-based epigenetic mechanisms regulating that expression. This webinar will review the importance of chromatin status to gene expression and provide examples of how studying both have led to a deeper understanding of biological phenotypes. Duration: 45 minutes followed by Q&A session. Schedule: Tuesday, June 4, 2013 at 1:00 PM Eastern    Status: Available Reserve

Copy Number: Variation and Alteration Analysis Strategies Structural changes in the genome result in either increasing or decreasing the number of copies present for different genomic loci. These changes can occur in the germ line and are heritable or can occur in somatic cells de novo. Alterations in gene copy number have profound effects on human biology and are associated with both developmental disorders and diseases. This webinar will begin with a general introduction of structural genomics followed by a review of historical approaches to measure changes in copy number. The webinar will conclude with an introduction of today’s most advanced techniques for measuring copy number and strategies to improve your genomic and translational studies. Learn how to apply these genomics tools to your research! Duration: 45 minutes followed by Q&A session. Schedule: Friday, June 7, 2013 at 1:00 PM Eastern    Status: Available Reserve Thursday, June 13, 2013 at 9:30 AM Eastern    Status: Available Reserve

Cancer and Inflammation: How Strong is the Link? This webinar will discuss the connection between inflammation and cancer, the major roles of inflammation in cancer development, and how oncogenic signaling can induce inflammation. State-of-the-art research tools will be introduced for analyzing inflammation response and contributors involved in cancer development and progression. Application examples will be presented. Come and learn how to use these tools in your research model system. Duration: 45 minutes followed by Q&A session. Schedule: Friday, May 31, 2013 at 1:00 PM Eastern    Status: Available Reserve Wednesday, June 19, 2013 at 1:00 PM Eastern    Status: Available Reserve

Biologically Interpret Your Gene Expression Analysis Results So you’ve got fold changes and p values from your gene expression analysis experiment. Now what? How do you make sense of the results to build a model that explains the biology and helps you design the next best experiment? Attend this webinar to learn how to take advantage of easy-to-use and free systems biology software to understand how genes might be co-expressed and co-regulated. Simply input your list of up-regulated and down-regulated genes. Then, use an automatically generated picture of the interactions between the differentially expressed genes and other regulatory genes to develop your hypotheses. Order the reagents needed for experiments designed to test them. Let us show you how to easily use your gene expression analysis results to interpret your biological experiment. Duration: 45 minutes followed by Q&A session. Schedule: Tuesday, May 28, 2013 at 9:30 AM Eastern    Status: Available Reserve Monday, June 24, 2013 at 1:00 PM Eastern    Status: Available Reserve

Toll-like Receptors in Inflammation "Toll-like receptors (TLRs) have been implicated in both innate and adaptive immunity-induced inflammation, thereby playing critical roles in providing the host with short- and long-term protection against infections.. This webinar will provide an overview of the roles that TLRs play in the regulation of Inflammation and solutions for studying these roles. An overview of TLR-mediated inflammation, the key signaling players involved in TLR-mediated inflammation, and the contribution of TLR-mediated inflammation to various physiological processes will be presented. Please come and learn about the state-of-the-art research tools for analyzing TLR-mediated inflammation and its regulation. " Duration: 45 minutes followed by Q&A session. Schedule: Wednesday, June 12, 2013 at 1:00 PM Eastern    Status: Available Reserve

Molecular Mechanisms of Neurodegeneration Common molecular mechanisms and pathways leading to neurodegeneration, such as Alzheimer’s Disease, Parkinson’s Disease, Huntington’s Disease or Multiple Sclerosis, will be discussed in this 45-minute webinar. Research and therapeutic strategies will be presented. Please join us to find out how these discoveries and tools can be used to facilitate your neurodegeneration research. Duration: 45 minutes followed by Q&A session. Schedule: Wednesday, May 29, 2013 at 9:30 AM Eastern    Status: Available Reserve Friday, June 14, 2013 at 1:00 PM Eastern    Status: Available Reserve

Functional analysis of miRNAs Understanding the function and targets of miRNA is important, but challenging research. Since one miRNA can potentially target several genes, finding and validating a specific miRNA-mRNA interaction often involves multiple molecular techniques. In this webinar we will highlight the use of miRNA mimics, inhibitors and target protectors to increase, decrease and adjust the cellular concentration of miRNA and disrupt specific miRNA-mRNA interactions. We will also present a ready-to-use screening tool for identifying miRNA targets in your samples. Lastly, we will show how miRNA expression data can be used to predict mRNA targets and how qPCR can be used to validate predicted interactions. Duration: 45 minutes followed by Q&A session. Schedule: Wednesday, June 26, 2013 at 1:00 PM Eastern    Status: Available Reserve

Next-generation sequencing, an introduction to technology and applications Next-generation sequencing (NGS) technology has revolutionized the study of the genomes, transcriptomes and epigenomes of any species. NGS employs massively parallel sequencing to poduce millions of sequences at once and delivering fast, inexpensive, and accurate genome information. This webinar will provide a technical overview of DNA/RNA preprocessing, template preparation, sequencing, and data analysis. It will also cover the applications for NGS technologies, including guidelines for how to select the technology that will best address your biological question of interest. Duration: 45 minutes followed by Q&A session. Schedule: Tuesday, June 4, 2013 at 9:30 AM Eastern    Status: Available Reserve

Next-generation sequencing for cancer research DNA sequence information is important for virtually all branches of biomedical research. Given the genetic aspect of the disease, cancer research is at the forefront of genome sequencing. Next-generation sequencing (NGS) has been adapted as a technology of choice for cataloguing mutations in multiple cancer types that can be translated into new diagnostic, prognostic, and therapeutic targets. In this webinar, we will discuss the most biologically efficient and cost-effective way to use NGS technology in cancer research. A new QIAGEN GeneRead DNAseq targeted enrichment system will be introduced that will allow you to quickly and reliably sequence your genes of interest. Duration: 45 minutes followed by Q&A session. Schedule: Tuesday, June 11, 2013 at 9:30 AM Eastern    Status: Available Reserve

Next-generation sequencing data analysis for genetic profiling Next-generation employs parallel sequencing process to produce millions of raw sequencing reads. Several steps including assembly, alignment, variant calling, filtering, and annotation are involved in converting raw sequencing reads into an understanding of functional biology. In this webinar, we will discuss the challenges of NGS data analysis and solution provided by the QIAGEN NGS data analysis portal for identifying genetic variants from cell, tissue, and FFPE samples. Using raw sequencing data, we will demonstrate how our web-based data analysis portal can be used to discover known as well as novel variants and derive a biological meaningful interpretation of NGS data. Duration: 45 minutes followed by Q&A session. Schedule: Tuesday, June 18, 2013 at 9:30 AM Eastern    Status: Available Reserve

Insulin resistance regulation and energy metabolism Insulin resistance and glucose intolerance are a hallmark of Type II diabetes and have been closely associated with obesity. PPARgamma and mTORC2 are two key regulators of insulin sensitivity. Regulation of glucose metabolism, along with the relationship between glucose, lipid and energy metabolism will be discussed in this webinar. Research solutions and application examples will be presented. Join us to learn how these technologies can be applied to your research. Duration: 45 minutes followed by Q&A session. Schedule: Friday, June 28, 2013 at 1:00 PM Eastern    Status: Available Reserve

Stem cell: overview and key stem cell signaling pathways The webinar will overview the key signaling pathways critical for embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) maintenance and differentiation, including Fibroblast Growth Factor, Hedgehog, Notch, TGFb and WNT. The major receptors and transcription factors of these signaling pathways, and stem-cell specific markers, and stem cell differentiation markers will also be presented. Qiagen has developed various techniques for profiling, screening and validating discrete biological processes during the various steps from reprogramming through terminal differentiation. Application examples will be presented. Come and learn how you can apply these tools to your research. Duration: 45 minutes followed by Q&A session. Schedule: Wednesday, May 29, 2013 at 1:00 PM Eastern    Status: Available Reserve Tuesday, June 18, 2013 at 1:00 PM Eastern    Status: Available Reserve

Biology-Focused Research: How QIAGEN Develops Scientifically Relevant Array-Based Products Every scientist knows the gene or genes that are the major focus of their research. However, the latest technologies allow and expect scientists to expand their research horizons, often requiring array-based products. Since it can be difficult to quickly identify 96 or 384 genes that could be relevant for your research, QIAGEN does the work for you. These 96 or 384 well products will allow you to quickly identify new research directions. In this webinar we will present how we design our biologically-focused arrays. We will present specific examples of cataloged array design from some of our key technologies (mRNA analysis, miRNA analysis, and mutation detection) using our unique bioinformatics tools combined with peer-reviewed literature. We will also cover our additional efforts that can help you choose the most relevant cataloged array for your research, and if necessary, how we can help you design the best custom array for your experimental needs. Duration: 45 minutes followed by Q&A session. Schedule: Tuesday, June 25, 2013 at 1:00 PM Eastern    Status: Available Reserve