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Online Seminars

SABiosciences is pleased to provide free, on-line educational resources covering relevant tools and techniques for gene and protein expression analysis, and cell-based gene function analysis. We offer live webinars, prerecorded and PDF formatted presentations.

See the instructions for the Web Seminars

May 2015
Monday
Tuesday
Wednesday
Thursday
Friday

1

4

Molecular Mechanisms of Neurodegeneration

5

Critical factors for successful multiplex endpoint PCR

QPCR Introduction

6

Exosomes in liquid biopsy

7

NGS: an introduction to technology and applications

8

Host–pathogen interactions

11

Circulating Biomarkers for Alzheimer’s Disease

12

Critical factors for successful multiplex qPCR

Coding & noncoding RNA analysis using qPCR

13

Maximize quantity and purity of exosomal RNA

14

Target NGS for cancer research

15

Microbiome: From identification to characterization

18

Non-Coding RNAs in Neurodegenerative Disorders

19

PCR Array Data Analysis Tutorial

20

Biomarker discovery in biofluids

21

Addressing the challenges of NGS workflow

22

Innate immune system

25

26

Service for biomarker research

27

Meeting the challenges of biomarker research

Integrated sample isolation solutions

28

Advance data Analysis with IVA and CLC Bio Cancer Workbench

29

Toll-like Receptors in Inflammation

Current Seminar Titles Available:

Focus Title
1.  PCR ArrayPCR Array Data Analysis Tutorial
2.  QPCR IntroductionIntroduction to Real Time PCR (Q-PCR/qPCR/qrt-PCR)
3.  miRNAsThe miRNA revolution: An introduction to microRNA biogenesis, function & analysis
4.  miRNAsmiRNA Quantitation: From experimental design through data analysis
5.  PCR Arrays in ImmunologyExploring the first line of defense: research tools for the innate immune system
6.  Inflammation and TLRToll-like Receptors in Inflammation
7.  Neurodegeneration MechanismsMolecular Mechanisms of Neurodegeneration
8.  miRNAsFunctional analysis of miRNAs
9.  Next-generation sequencingNext-generation sequencing, an introduction to technology and applications
10.  Microbial PCR ArraysMicrobiome: From identification to characterization
11.  miRNAsMicroRNA biomarker discovery - overcoming limiting sample material
12.  Host–Pathogen InteractionsHost–Pathogen Interactions: Molecular Basis and Host Defense Mechanisms
13.  Next-generation sequencingNext-Generation Sequencing — Targeted Enrichment Technology in Cancer Research
14.  Next-generation sequencingAdvancing NGS Data Analysis and Interpretation: CLC Cancer Research Workbench and Ingenuity Variant Analysis
15.  Liquid BiopsiesExosome Research – Maximize Quantity and Purity of Exosomal RNA
16.  Next-generation sequencingAddressing the challenges of NGS workflow: sample preparation, quality control and automation
17.  Single cell analysisSingle cell analysis - from sample to insight
18.  Single cell analysisChallenges and solutions of single cell analysis
19.  Service CoreAccelerate your discovery with QIAGEN service solutions for biomarker research
20.  NeurodegenerationCirculating Biomarkers for Alzheimer’s Disease
21.  NeurodegenerationThe Central Roles of Non-Coding RNAs in Neurodegenerative Disorders
22.  Sample IsolationIntegrated sample isolation for cancer, immunology, and microbiome studies
23.  Multiplex PCRPractical hints and new solutions for successful real-time PCR studies
24.  Multiplex PCRCritical factors for successful multiplex endpoint PCR
25.  Multiplex PCRCritical Factors for Successful Multiplex Real-Time PCR
26.  PCR ArraysAdvanced Real-Time PCR Array Technology – Coding & Noncoding RNA Expression Analysis
27.  QuantiNova PCR kit Novel in-process monitoring tools for improving accuracy and reproducibility of qPCR results
28.  RNA IsolationBack to Basics: Fundamental Concepts and Special Considerations in RNA Isolation
29.  QIAmp circulating NA kitGetting Past the Pitfalls: Best Practices in Pre-analytical Sample Handling for Free Circulating DNA Isolation
30.  Cignal Reporter AssaysCell-based Reporter Assays: Measure 45 Signaling Pathway Activity in Any Cell Type
31.  miscript miRNA PCR ArraysCirculating Biomarker Discovery in Biofluids: from Sample to Biomarker
32.  PAXgene RNA KitsWhole Blood RNAseq: A standardized and validated workflow for robust whole transcriptome
33.  lncRNA PCR ArraysLong Non-coding RNAs (lncRNAs) as Novel Circulating Biomarkers: lncRNA Profiling and Detection
34.  Single cell analysisSingle-Cell Mutation Detection – Overcoming Challenges in Single-Cell Analysis

PCR Array Data Analysis Tutorial

With a live demonstration using actual PCR array data, learn how easy it is to use our data analysis Web portal to calculate fold-differences in gene expression from your raw real-time PCR threshold cycles.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, June 23, 2015 at 9:30 AM Eastern    Status: Available Reserve

Introduction to Real Time PCR (Q-PCR/qPCR/qrt-PCR)

Real-time polymerase chain reaction (qPCR) is the most sensitive and reliable method for the detection and quantification of nucleic acids. This 45-minute webinar introduces the concepts of real-time PCR and how to conduct a real-time PCR assay. The topics that will be covered include an overview of real-time PCR chemistries, protocols, quantification methods, real-time PCR applications, and factors for success. This webinar is for both beginners that are new to real-time PCR as well as experienced users.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, June 9, 2015 at 9:30 AM Eastern    Status: Available Reserve

The miRNA revolution: An introduction to microRNA biogenesis, function & analysis

miRNA plays a critical role in many biological processes such as differentiation and development, cell signaling, response to infection, and other challenges. Recent studies have shown overwhelming evidence that aberration in miRNA expression is causal or an indicator of many disease processes including cancer. Currently there is a race to find and develop miRNAs as biomarkers and therapeutics. To win the race, it is important to have a strong understanding of miRNA and the challenges of miRNA research. The webinar will cover the biology of miRNA, the key challenges associated with miRNA research, and the latest advances in miRNA research technology.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, June 10, 2015 at 1:00 PM Eastern    Status: Available Reserve

miRNA Quantitation: From experimental design through data analysis

miRNAs play a critical role in many biological processes including neuronal cell fate, hematology, apoptosis and developmental biology. Increasing evidence has shown that aberrations in microRNA regulation are associated with many disease processes. This webinar will discuss the challenges associated with miRNA quantification by real-time PCR, and the latest strategies for miRNA quantification and profiling. The critical parameters of profiling miRNA gene expression will also be presented. Come and learn how you can apply these tools in your miRNA research!

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, June 17, 2015 at 1:00 PM Eastern    Status: Available Reserve

Exploring the first line of defense: research tools for the innate immune system

The innate immune system executes crucial and unique functions for host defense against infection. This webinar provides an overview of the most important cellular and molecular players of innate immunity and discusses their functions in a variety of disease states. We also introduce research technologies for exploring innate immune activity in your system through profiling of gene expression and cytokines, signal transduction pathway analysis and more, all in the context.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, June 17, 2015 at 9:30 AM Eastern    Status: Available Reserve

Toll-like Receptors in Inflammation

"Toll-like receptors (TLRs) have been implicated in both innate and adaptive immunity-induced inflammation, thereby playing critical roles in providing the host with short- and long-term protection against infections.. This webinar will provide an overview of the roles that TLRs play in the regulation of Inflammation and solutions for studying these roles. An overview of TLR-mediated inflammation, the key signaling players involved in TLR-mediated inflammation, and the contribution of TLR-mediated inflammation to various physiological processes will be presented. Please come and learn about the state-of-the-art research tools for analyzing TLR-mediated inflammation and its regulation. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Friday, May 29, 2015 at 1:00 PM Eastern    Status: Available Reserve
Wednesday, June 24, 2015 at 9:30 AM Eastern    Status: Available Reserve

Molecular Mechanisms of Neurodegeneration

Common molecular mechanisms and pathways leading to neurodegeneration, such as Alzheimer’s Disease, Parkinson’s Disease, Huntington’s Disease or Multiple Sclerosis, will be discussed in this 45-minute webinar. Research and therapeutic strategies will be presented. Please join us to find out how these discoveries and tools can be used to facilitate your neurodegeneration research.

Duration: 45 minutes followed by Q&A session.

Schedule:

Friday, June 12, 2015 at 1:00 PM Eastern    Status: Available Reserve

Functional analysis of miRNAs

Understanding the function and targets of miRNA is important, but challenging research. Since one miRNA can potentially target several genes, finding and validating a specific miRNA-mRNA interaction often involves multiple molecular techniques. In this webinar we will highlight the use of miRNA mimics, inhibitors and target protectors to increase, decrease and adjust the cellular concentration of miRNA and disrupt specific miRNA-mRNA interactions. We will also present a ready-to-use screening tool for identifying miRNA targets in your samples. Lastly, we will show how miRNA expression data can be used to predict mRNA targets and how qPCR can be used to validate predicted interactions.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, June 24, 2015 at 1:00 PM Eastern    Status: Available Reserve

Next-generation sequencing, an introduction to technology and applications

Next-generation sequencing (NGS) technology has revolutionized the study of the genomes, transcriptomes and epigenomes of any species. NGS employs massively parallel sequencing to poduce millions of sequences at once and delivering fast, inexpensive, and accurate genome information. This webinar will provide a technical overview of DNA/RNA preprocessing, template preparation, sequencing, and data analysis. It will also cover the applications for NGS technologies, including guidelines for how to select the technology that will best address your biological question of interest.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, June 1, 2015 at 9:30 AM Eastern    Status: Available Reserve

Microbiome: From identification to characterization

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, June 10, 2015 at 9:30 AM Eastern    Status: Available Reserve

MicroRNA biomarker discovery - overcoming limiting sample material

This webinar presents an integrated, PCR-based system that reduces the amount of sample required for full miRNome profiling and provides unparalleled reproducibility and precision. This advance opens up new possibilities for biomarker development.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, June 3, 2015 at 1:00 PM Eastern    Status: Available Reserve

Host–Pathogen Interactions: Molecular Basis and Host Defense Mechanisms

Host–pathogen interactions are strikingly complex during infection. This webinar provides an overview of the molecular basis of these intricate interactions; the impact of microbiota on innate and adaptive immunity, metabolism, and insulin resistance; and host defense mechanisms, including newly reported inflammasome-mediated responses and miRNA-mediated responses. Various research tools will be introduced to simplify and streamline each step of studying the host response, enabling analysis of gene expression and regulation, epigenetic modification, genotyping, and signal transduction pathway activation.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, June 3, 2015 at 9:30 AM Eastern    Status: Available Reserve

Next-Generation Sequencing — Targeted Enrichment Technology in Cancer Research

Genetic variance analysis plays a critical role cancer diagnosis, prognosis and treatment of cancer. Low sample volume and poor DNA quality are nagging issues with cancer samples making the results unreliable and expensive. This webinar discusses the most biologically efficient, cost-effective method for successful NGS. The GeneRead DNA QuantiMIZE Kits enable determination of the optimum conditions for targeted enrichment of DNA isolated from biological samples, while the GeneRead DNAseq Panels V2 allow you to quickly and reliably deep sequence your genes of interest. Applications in translational and clinical research will be highlighted.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, June 15, 2015 at 9:30 AM Eastern    Status: Available Reserve

Advancing NGS Data Analysis and Interpretation: CLC Cancer Research Workbench and Ingenuity Variant Analysis

Deep-sequencing technologies are enabling critical insights into all aspects of cancer genomics. This webinar will discuss two comprehensive informatics solutions — the CLC Cancer Research Workbench and Ingenuity Knowledge Base Variant Analysis platforms. These NGS analysis platforms provide a user-friendly and customizable solution, enabling you to easily narrow down your focus to causal variants and detect and annotate low-frequency somatic variants. We will show the intuitive user interface of CLC Cancer Research Workbench and demonstrate how the rich biological content from Ingenuity Knowledge Base helps you rapidly identify critical variants in your samples.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, June 29, 2015 at 1:00 PM Eastern    Status: Available Reserve

Exosome Research – Maximize Quantity and Purity of Exosomal RNA

Recent years have seen an increased interest in the significance of RNA and other molecules carried by exosomes and other extracellular vesicles (EVs). Specifically, these vesicles may be the key to identifying circulating biomarkers. The major limitation of this strategy is the ability to isolate these exosomes in a standardized manner. Until now, methods for purifying exosomes for RNA isolation have been time-consuming and inconsistent. QIAGEN has developed solutions to help isolate and enrich exosomes and other extracellular vesicles, to allow the detection of low-abundance RNAs that are in circulation. The seminar will describe new solutions for sample preparation and discuss how to maximize quantity and purity of low-abundance fragments.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, June 15, 2015 at 1:00 PM Eastern    Status: Available Reserve

Addressing the challenges of NGS workflow: sample preparation, quality control and automation

The rapid technological advances in NGS have revolutionized the field of genomics, and broaden the applications from basic research, translational to clinical research. However, there exist enormous challenges in terms of sample preparation, sample quality control, and library preparation, which affect sequencing quality. This webinar will focus on these challenges and discuss various solutions to tackle these challenges and maximize your NGS workflow.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, June 8, 2015 at 1:00 PM Eastern    Status: Available Reserve

Single cell analysis - from sample to insight

What can you do from a single cell? Actually, a lot! Beginning with the genome, you can discover new biomarkers by identifying new genetic variances and their association with specific diseases, including cancers. Moving on to RNA, the recent advances in RNA sequencing technology have made single-cell transcriptomics a possibility. Along with these possibilities come challenges that start from the moment you get the sample to the final step of gaining insights into the cell. This webinar will provide an overview on the multiple steps involved as you move from sample acquisition to analysis and data interpretation in different sample types.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, June 11, 2015 at 1:00 PM Eastern    Status: Available Reserve

Challenges and solutions of single cell analysis

Cell heterogeneity plays a central role both in normal development and in disease. Single cell genomic analysis is essential to dissect the genotypic and phenotypic variability, but the small amounts of DNA and mRNA present unique challenges. In this webinar, you will learn about current approaches for single cell genome and transcriptome analysis.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, June 18, 2015 at 1:00 PM Eastern    Status: Available Reserve

Accelerate your discovery with QIAGEN service solutions for biomarker research

Development of preclinical biomarkers can have several pitfalls, often requiring a large investment in instruments to process samples, time to develop assays, and specialties in data analysis and biomarker algorithm design. QIAGEN’s life sciences service core can provide researchers with the tools, knowledge and specialties to help develop the next generation of biomarkers for cancer, toxicology and inflammation research. This seminar will highlight QIAGEN’s service capabilities in sample isolation, microarray and NGS-sequencing, qPCR panel, and custom assay development and bioinformatics as we look at the identification of potential biomarkers and gene signatures. We will discuss the applications of QIAGEN Service Core in microRNA discovery for toxicology markers in serum and plasma and in identification of RNA signatures for tumor stratification. Join us and learn how you can accelerate your research with QIAGEN service solutions.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, June 30, 2015 at 9:30 AM Eastern    Status: Available Reserve

Circulating Biomarkers for Alzheimer’s Disease

Alzheimer's disease (AD) is a complex neurodegenerative disorder. Circulating miRNAs hold great promise in the discovery of non-invasive and novel biomarkers for AD diagnosis and prognosis. This webinar will review the role of miRNAs in AD and discuss current progress in biomarker discovery. We will present various tools for pathway-focused and genomewide miRNA expression profiling, miRNA functional studies and target identification.

Duration: 45 minutes followed by Q&A session.

Schedule:

Friday, June 26, 2015 at 1:00 PM Eastern    Status: Available Reserve

The Central Roles of Non-Coding RNAs in Neurodegenerative Disorders

Non-coding RNAs (ncRNAs), especially microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) have shown aberrant expression profiles in neurodegenerative disorders. This webinar will review the roles of lncRNAs and their mechanisms of action in the regulation of neurodegeneration. Their potential as circulating biomarkers will be discussed. Novel solutions to isolate RNAs from blood and cerebral spinal fluid (CSF) will be discussed and a new qPCR-based lncRNA platform for lncRNA detection and profiling will also be introduced.

Duration: 45 minutes followed by Q&A session.

Schedule:

Friday, June 19, 2015 at 1:00 PM Eastern    Status: Available Reserve

Integrated sample isolation for cancer, immunology, and microbiome studies

The central dogma of molecular biology, genes to messenger to protein, guides a principle of molecular biology research: that to fully understand gene expression, one should look at each level of the process. However, limitations in sample amount and time can make isolating DNA, RNA, and protein a tricky prospect. In this webinar, we will discuss how you can simultaneously extract DNA and total RNA, or DNA, RNA, and protein, from the same sample. Case studies using this technology in cancer, immunology, and microbiology studies will also be discussed. Come join us to learn how you can simplify your sample preparation and enhance your molecular biology studies!

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, June 22, 2015 at 9:30 AM Eastern    Status: Available Reserve

Practical hints and new solutions for successful real-time PCR studies

In this webinar we will cover the following topics which are critical steps for efficient and precise gene expression studies using real-time PCR technology: - Effect of RNA integrity on real-time PCR results – tips to achieve a true RNA profiling suitable for real-time PCR studies - Improved methods for cDNA synthesis, optimized for real-time PCR - Real-time PCR analysis • Real-time PCR essentials and background information on different quantification strategies • SYBR Green real-time PCR – factors influencing specificity • Introduction to probe technology • New, fast and efficient real-time PCR solutions

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, June 2, 2015 at 1:00 PM Eastern    Status: Available Reserve

Critical factors for successful multiplex endpoint PCR

Multiplex endpoint PCR is a powerful tool for genotyping and many other applications. The ability to amplify and detect several DNA targets in the same reaction offers many benefits. It enables generation of more data out of less sample material, which is especially beneficial in routines with limited and precious sample material. It also offers the opportunity to run internal controls in every reaction, thus increasing the overall reliability of data acquisition. Most importantly of course, switching PCR-based routine lab testing to multiplex PCR offers the opportunity to substantially save on time, materials and cost. QIAGEN’s multiplex PCR chemistry is optimized for reliable amplification of many different templates with high variability in copy numbers. Thus it enables very quick establishment of a new lab routine and instant success for your multiplex PCR strategy. There is a set of critical factors which we recommend to be regarded for planning and performing this kind of PCR. These will be discussed in detail in the webinar. Additionally, our multiplex PCR chemistry has recently been gaining increasing popularity among scientists who are utilizing it for their next-generation sequencing workflows. We will also briefly touch on this topic.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, June 9, 2015 at 1:00 PM Eastern    Status: Available Reserve

Critical Factors for Successful Multiplex Real-Time PCR

Multiplex real-time PCR is a powerful tool for gene expression analysis, viral load monitoring, genotyping, and many other applications. The ability to amplify and detect several genomic DNA, cDNA, or RNA targets in the same reaction offers many benefits: • Conservation of precious samples – more quantification data per sample • Increased throughput – more targets analyzed per run on a cycler • Reliable results – no well-to-well variability due to co-amplification of internal control • Reduced costs – save time and reagents The QuantiFast Multiplex PCR and RT-PCR kits are optimized for reliable amplification of many different templates despite a high variability in abundance. Thus they enable successful amplification of multiple targets on the first attempt without optimization. This webinar explains the principles of the QIAGEN multiplex technologies and shows data demonstrating the exceptional multiplex real-time PCR performance of the QuantiFast Multiplex kits.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, June 16, 2015 at 1:00 PM Eastern    Status: Available Reserve

Advanced Real-Time PCR Array Technology – Coding & Noncoding RNA Expression Analysis

This webinar presents a simple and accurate real-time PCR system for relevant biological pathway- & disease-focused mRNA and long non-coding RNA (lncRNA) expression profiling. Learn about the stringent performance built into the technology to insure its sensitivity, specificity, reproducibility, and reliability. Application examples will be presented.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, June 16, 2015 at 9:30 AM Eastern    Status: Available Reserve

Novel in-process monitoring tools for improving accuracy and reproducibility of qPCR results

The increasing demand for streamlined, monitored, and ultrafast qPCR procedures requires high-performance, real-time quantitative RT and PCR chemistries. Particularly procedures utilizing generic kits for gene expression analysis should include in-process safety measures to avoid variables and control accuracy of procedures and results. This webinar presents innovative solutions for one-step and two-step RT-PCR that significantly enhance performance and reliability in qRT-PCR. The new QuantiNova kit family offers a combination of various integrated safety features to remove variables and prevent artifacts. Internal Control RNA, removal of genomic DNA, room temperature set up capability for RT-PCR, and a built-in visual pipetting control verify accurate procedures, ensuring reliable gene expression profiling. This webinar explains the principles of the technologies and shows data demonstrating performance in qRT-PCR. Find out how you can verify accurate performance in qRT-PCR and improve your results.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, June 23, 2015 at 1:00 PM Eastern    Status: Available Reserve

Back to Basics: Fundamental Concepts and Special Considerations in RNA Isolation

How are your RNA yields? Some sample types present special challenges in RNA purification and analysis. In this webinar, we will discuss and provide tips for the following topics: • The basic methods and challenges of RNA purification • Special considerations for challenging sample types • Isolating miRNA and extracellular RNA • Performing RNA quality checks Join us to learn how you can apply these practical tips to high quality and quantity of RNA.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, June 1, 2015 at 1:00 PM Eastern    Status: Available Reserve

Getting Past the Pitfalls: Best Practices in Pre-analytical Sample Handling for Free Circulating DNA Isolation

Isolation and analysis of free circulating DNA has many challenges. Stabilization and processing samples before the actual isolation has a large impact on downstream results. Best practices for handling samples will be discussed during this webinar. In addition, questions such as how much blood should be taken, what are the best protocols to prepare plasma and what kind of controls can be used will be answered.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, June 2, 2015 at 9:30 AM Eastern    Status: Available Reserve

Cell-based Reporter Assays: Measure 45 Signaling Pathway Activity in Any Cell Type

Would you like to measure signaling pathway activity in your favorite cell? Learn how to successfully apply convenient and robust reporter assays to your RNA interference, gene over-expression, protein, or small molecule studies. The Cignal Reporter Assays are an excellent tool for studying pathway signaling activity in cells that are amenable to transfection, available for studying numerous pathways including (ROS, Wnt, NF-kB, Notch, cAMP/PKA, TGFbeta, and The Cignal Lenti Reporter Assays combines the power of a lentiviral delivery system with our robust transcription factor reporter technology, enabling you to study signal pathways in virtually any cell type. A technology overview, protocol tutorial, and application examples will be presented. Join us and learn how you can apply this advance tool to your research!

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, June 4, 2015 at 1:00 PM Eastern    Status: Available Reserve

Circulating Biomarker Discovery in Biofluids: from Sample to Biomarker

Developing novel non-invasive biomarkers holds great promise in improving detection and prognostic evaluation of cancer. Circulating microRNAs are potential candidates, as they reflect the key characteristics of cancer biomarkers and are highly stable in biofluids. This webinar presents an integrated system for circulating biomarker discovery from sample to insight. Advanced techniques for exosomal miRNA isolation and miRNA detection will be discussed.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, June 8, 2015 at 9:30 AM Eastern    Status: Available Reserve

Whole Blood RNAseq: A standardized and validated workflow for robust whole transcriptome

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, June 22, 2015 at 1:00 PM Eastern    Status: Available Reserve

Long Non-coding RNAs (lncRNAs) as Novel Circulating Biomarkers: lncRNA Profiling and Detection

lncRNAs are dysregulated in various cancer types and closely related to tumorigenesis, metastasis, and prognosis or diagnosis. This webinar will discuss the regulation and functional roles of lncRNA in cancer. A new qPCR-based lncRNA platform for lncRNA detection and profiling will be introduced. An application for serum lncRNA detection as a potential biomarker of lung cancer will also be highlighted.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, June 29, 2015 at 9:30 AM Eastern    Status: Available Reserve

Single-Cell Mutation Detection – Overcoming Challenges in Single-Cell Analysis

Genetic alteration is the driving factor behind various biological processes such as developmental malformation, evolution and cancer. Recent findings of genomic heterogeneity among supposedly homogeneous cell populations such as tumor cells demands genomic characterization at the individual cell level to better understand the underlying biology. Single nucleotide variation (SNV) analysis by next-generation sequencing (NGS) requires relatively high coverage that often necessitates targeted enrichment or amplification to focus on sites of interest. In this webinar, we will present a multiplex PCR-based targeted enrichment sequencing method for detecting mutations in single cells isolated from two colon cancer cell lines, Lovo and HT29. We will discuss the technologies along the workflow as well as the challenges and how to address them.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, June 25, 2015 at 1:00 PM Eastern    Status: Available Reserve

Note: Viewers will be asked to register before viewing the previously recorded webinars.