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Online Seminars

SABiosciences is pleased to provide free, on-line educational resources covering relevant tools and techniques for gene and protein expression analysis, and cell-based gene function analysis. We offer live webinars, prerecorded and PDF formatted presentations.

See the instructions for the Web Seminars

March 2015
Monday
Tuesday
Wednesday
Thursday
Friday

2

Analysis of somatic BRCA1 and BRCA2 variants

3

Cancer Stem Cells

4

Exosomes in liquid biopsy

5

Research Solutions for Liquid Biopsies

6

9

Host–pathogen interactions

10

NGS: an introduction to technology and applications

11

Maximize quantity and purity of exosomal RNA

12

Genomic Biomarker Discovery

13

QPCR Introduction

long noncoding RNA (lncRNA)

16

Microbiome: From identification to characterization

17

Addressing the challenges of NGS workflow

18

Biomarker discovery in biofluids

19

Circulating Biomarkers - PreAMP technology

20

PCR Arrays for Pathway Analysis

23

Research tools for the innate immune system

24

Target NGS for cancer research

25

Meeting the challenges of biomarker research

26

lncRNAs in Cancer & miRNA Regulation

27

PCR Array Data Analysis Tutorial

Optimize NGS conditions based on your DNA sample

30

Toll-like Receptors in Inflammation

31

NGS data anlaysis

Current Seminar Titles Available:

Focus Title
1.  PCR ArrayPCR Array Data Analysis Tutorial
2.  PCR ArrayPCR Arrays: The Real Pioneer in Real-Time PCR Analysis of Biological Pathways
3.  QPCR IntroductionIntroduction to Real Time PCR (Q-PCR/qPCR/qrt-PCR)
4.  PCR Arrays in ImmunologyExploring the first line of defense: research tools for the innate immune system
5.  Inflammation and TLRToll-like Receptors in Inflammation
6.  Next-generation sequencingNext-generation sequencing, an introduction to technology and applications
7.  Next-generation sequencingNext-generation sequencing data analysis for genetic profiling
8.  Microbial PCR ArraysMicrobiome: From identification to characterization
9.  Host–Pathogen InteractionsHost–Pathogen Interactions: Molecular Basis and Host Defense Mechanisms
10.  PreAMP for qBiomarker somatic mutationCirculating Biomarkers: New solutions for RNA and DNA
11.  lncRNANew advances in long noncoding RNA (lncRNA) research
12.  Next-generation sequencingNext-Generation Sequencing — Targeted Enrichment Technology in Cancer Research
13.  Liquid BiopsiesStep by Step: From a Liquid Biopsy to a Genomic Biomarker
14.  Liquid BiopsiesRole of Long Non-coding RNAs (lncRNAs) in Cancer and microRNA Regulation
15.  Liquid BiopsiesExosome Research – Maximize Quantity and Purity of Exosomal RNA
16.  miRNA BiomarkerBiomarker Discovery in Biofluids: from Sample to Biomarker
17.  miRNA BiomarkerMeeting the Challenges of Biomarker Research: miRNA Function, Profiling and Data Analysis
18.  Next-generation sequencingAddressing the challenges of NGS workflow: sample preparation, quality control and automation
19.  Next-generation sequencingNot All DNA is Created Equal: Optimize NGS Conditions Based on Your DNA Sample

PCR Array Data Analysis Tutorial

With a live demonstration using actual PCR array data, learn how easy it is to use our data analysis Web portal to calculate fold-differences in gene expression from your raw real-time PCR threshold cycles.

Duration: 45 minutes followed by Q&A session.

Schedule:

Friday, March 27, 2015 at 1:00 PM Eastern    Status: Available Reserve

PCR Arrays: The Real Pioneer in Real-Time PCR Analysis of Biological Pathways

In this 45-minute Webinar, see how the PCR array has led the way toward simple and accurate real-time PCR analysis of relevant biological pathways and diseases. Learn about the stringent performance built into the technology to ensure its sensitivity, specificity, reproducibility, and reliability. Find out how researchers like you have applied PCR arrays to their cancer, immunology, and toxicology research.

Duration: 45 minutes followed by Q&A session.

Schedule:

Friday, March 20, 2015 at 1:00 PM Eastern    Status: Available Reserve

Introduction to Real Time PCR (Q-PCR/qPCR/qrt-PCR)

Real-time polymerase chain reaction (qPCR) is the most sensitive and reliable method for the detection and quantification of nucleic acids. This 45-minute webinar introduces the concepts of real-time PCR and how to conduct a real-time PCR assay. The topics that will be covered include an overview of real-time PCR chemistries, protocols, quantification methods, real-time PCR applications, and factors for success. This webinar is for both beginners that are new to real-time PCR as well as experienced users.

Duration: 45 minutes followed by Q&A session.

Schedule:

Friday, March 13, 2015 at 1:00 PM Eastern    Status: Available Reserve

Exploring the first line of defense: research tools for the innate immune system

The innate immune system executes crucial and unique functions for host defense against infection. This webinar provides an overview of the most important cellular and molecular players of innate immunity and discusses their functions in a variety of disease states. We also introduce research technologies for exploring innate immune activity in your system through profiling of gene expression and cytokines, signal transduction pathway analysis and more, all in the context.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, March 23, 2015 at 9:30 AM Eastern    Status: Available Reserve

Toll-like Receptors in Inflammation

"Toll-like receptors (TLRs) have been implicated in both innate and adaptive immunity-induced inflammation, thereby playing critical roles in providing the host with short- and long-term protection against infections.. This webinar will provide an overview of the roles that TLRs play in the regulation of Inflammation and solutions for studying these roles. An overview of TLR-mediated inflammation, the key signaling players involved in TLR-mediated inflammation, and the contribution of TLR-mediated inflammation to various physiological processes will be presented. Please come and learn about the state-of-the-art research tools for analyzing TLR-mediated inflammation and its regulation. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, March 30, 2015 at 9:30 AM Eastern    Status: Available Reserve

Next-generation sequencing, an introduction to technology and applications

Next-generation sequencing (NGS) technology has revolutionized the study of the genomes, transcriptomes and epigenomes of any species. NGS employs massively parallel sequencing to poduce millions of sequences at once and delivering fast, inexpensive, and accurate genome information. This webinar will provide a technical overview of DNA/RNA preprocessing, template preparation, sequencing, and data analysis. It will also cover the applications for NGS technologies, including guidelines for how to select the technology that will best address your biological question of interest.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, March 10, 2015 at 1:00 PM Eastern    Status: Available Reserve

Next-generation sequencing data analysis for genetic profiling

Next-generation employs parallel sequencing process to produce millions of raw sequencing reads. Several steps including assembly, alignment, variant calling, filtering, and annotation are involved in converting raw sequencing reads into an understanding of functional biology. In this webinar, we will discuss the challenges of NGS data analysis and solution provided by the QIAGEN NGS data analysis portal for identifying genetic variants from cell, tissue, and FFPE samples. Using raw sequencing data, we will demonstrate how our web-based data analysis portal can be used to discover known as well as novel variants and derive a biological meaningful interpretation of NGS data.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, March 31, 2015 at 1:00 PM Eastern    Status: Available Reserve

Microbiome: From identification to characterization

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, March 16, 2015 at 9:30 AM Eastern    Status: Available Reserve

Host–Pathogen Interactions: Molecular Basis and Host Defense Mechanisms

Host–pathogen interactions are strikingly complex during infection. This webinar provides an overview of the molecular basis of these intricate interactions; the impact of microbiota on innate and adaptive immunity, metabolism, and insulin resistance; and host defense mechanisms, including newly reported inflammasome-mediated responses and miRNA-mediated responses. Various research tools will be introduced to simplify and streamline each step of studying the host response, enabling analysis of gene expression and regulation, epigenetic modification, genotyping, and signal transduction pathway activation.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, March 9, 2015 at 9:30 AM Eastern    Status: Available Reserve

Circulating Biomarkers: New solutions for RNA and DNA

The promise of circulating biomarkers is to enable minimally-invasive monitoring of specific molecules that yield predictive or prognostic information. However, that promise cannot be achieved until reliable screening and profiling tools become available to identify specific RNA or DNA molecules with informational value. This process is complicated by the dilution of these valuable molecules by the vast excess of nucleic acids that are not changing or not relevant as biomarkers. QIAGEN has developed solutions to help enrich, amplify and profile nucleic acids that are in circulation, such as tumor-derived DNA that carries a specific mutation or miRNAs that establish a specific expression signature. The webinar will describe solutions from sample preparation to molecule detection that will screen, profile and monitor putative biomarkers. The application examples will highlight new tools for exosomes, cfDNA, and circulating miRNA.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, March 19, 2015 at 1:00 PM Eastern    Status: Available Reserve

New advances in long noncoding RNA (lncRNA) research

"Recent improvements in RNA-sequencing have led to the discovery of thousands of RNAs that do not contain any open reading frames, termed “long noncoding RNA (lncRNA)”. lncRNA expression is thought to be important for the regulation of gene (mRNA) expression through several different mechanisms, some involving competition with transcription and others involving the recruitment of specific epigenetic factors to promoter regions. lncRNA may also indirectly affect gene expression by interacting with miRNA and other cellular factors. In this webinar, we will review what is known about lncRNA and use application examples in cancer research to illustrate how expression profiling is being used to identify lncRNA biomarkers from cells, tissues, blood, and exosomes. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Friday, March 13, 2015 at 11:00 AM Eastern    Status: Available Reserve

Next-Generation Sequencing — Targeted Enrichment Technology in Cancer Research

Genetic variance analysis plays a critical role cancer diagnosis, prognosis and treatment of cancer. Low sample volume and poor DNA quality are nagging issues with cancer samples making the results unreliable and expensive. This webinar discusses the most biologically efficient, cost-effective method for successful NGS. The GeneRead DNA QuantiMIZE Kits enable determination of the optimum conditions for targeted enrichment of DNA isolated from biological samples, while the GeneRead DNAseq Panels V2 allow you to quickly and reliably deep sequence your genes of interest. Applications in translational and clinical research will be highlighted.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, March 24, 2015 at 1:00 PM Eastern    Status: Available Reserve

Step by Step: From a Liquid Biopsy to a Genomic Biomarker

Liquid biopsies enable us to monitor the evolution of genetic aberrations in primary tumors as they shed tumor cells into the circulation. The major limitation of this strategy is the ability to detect these low-frequency genetic aberrations in a consistent manner. The webinar will cover the challenges and solutions associated with each step, beginning with liquid biopsy and moving towards finding a new biomarker.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, March 12, 2015 at 1:00 PM Eastern    Status: Available Reserve

Role of Long Non-coding RNAs (lncRNAs) in Cancer and microRNA Regulation

It is becoming evident that long non-coding RNAs (lncRNA) play important roles in gene regulation. Many lncRNAs are dysregulated in various cancer types and closely related to tumorigenesis, metastasis, and prognosis or diagnosis. This webinar will discuss the regulation and functional roles of lncRNA in cancers, with a particular focus on how lncRNAs impact microRNA expression and function. A new qPCR-based lncRNA platform for lncRNA detection and profiling will be introduced. An application for serum lncRNA detection as a potential biomarker of lung cancer will also be highlighted.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, March 26, 2015 at 1:00 PM Eastern    Status: Available Reserve

Exosome Research – Maximize Quantity and Purity of Exosomal RNA

Recent years have seen an increased interest in the significance of RNA and other molecules carried by exosomes and other extracellular vesicles (EVs). Specifically, these vesicles may be the key to identifying circulating biomarkers. The major limitation of this strategy is the ability to isolate these exosomes in a standardized manner. Until now, methods for purifying exosomes for RNA isolation have been time-consuming and inconsistent. QIAGEN has developed solutions to help isolate and enrich exosomes and other extracellular vesicles, to allow the detection of low-abundance RNAs that are in circulation. The seminar will describe new solutions for sample preparation and discuss how to maximize quantity and purity of low-abundance fragments.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, March 11, 2015 at 9:30 AM Eastern    Status: Available Reserve

Biomarker Discovery in Biofluids: from Sample to Biomarker

Developing novel non-invasive biomarkers holds great promise in improving detection and prognostic evaluation of cancer. Circulating microRNAs are promising candidates, as they reflect the key characteristics of cancer biomarkers and are highly stable in biofluids. This webinar presents an integrated system for circulating biomarker discovery from sample to insight. Advanced techniques for exosomal miRNA isolation and miRNA detection will be discussed.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, March 18, 2015 at 9:30 AM Eastern    Status: Available Reserve

Meeting the Challenges of Biomarker Research: miRNA Function, Profiling and Data Analysis

Recent studies have shown strong evidence that aberration in microRNA regulation is associated with many disease processes including cancer and neurodegenerative diseases, indicating the huge potential of miRNAs as diagnostic and prognostic biomarkers. This webinar addresses the current challenges associated with miRNA quantification by real-time PCR, and the latest strategies for miRNA quantification and profiling. The critical parameters of profiling miRNA gene expression and a free web-based miRNA data analysis tool will also be presented.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, March 25, 2015 at 9:30 AM Eastern    Status: Available Reserve

Addressing the challenges of NGS workflow: sample preparation, quality control and automation

The rapid technological advances in NGS have revolutionized the field of genomics, and broaden the applications from basic research, translational to clinical research. However, there exist enormous challenges in terms of sample preparation, sample quality control, and library preparation, which affect sequencing quality. This webinar will focus on these challenges and discuss various solutions to tackle these challenges and maximize your NGS workflow.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, March 17, 2015 at 1:00 PM Eastern    Status: Available Reserve

Not All DNA is Created Equal: Optimize NGS Conditions Based on Your DNA Sample

Formalin-fixed paraffin-embedded (FFPE) tissue archives are an invaluable resource for the molecular characterization of disease using next-generation sequencing (NGS). Unfortunately, heavy damage and fragmentation from the preservation process often results in samples that cannot be sequenced. Commonly used methods, such as fluorometry and spectrometry, are extremely limited in utility for NGS applications because they measure total amounts of DNA, and do not differentiate between functional (amplifiable) and non-functional (non-amplifiable) DNA, leading to inaccurate quantification and qualification of DNA samples, and poor sequencing of these samples. Real-time PCR, however, has the ability to accurately quantify and qualify DNA samples for NGS analysis, leading to improved library yields and successful NGS runs. Attend this webinar to learn how to use a novel qPCR-based method for quantifying and qualifying FFPE samples for NGS, without the need for serial dilutions or multiple standards. This method also optimizes conditions for successful targeted sequencing of low-quality DNA samples. By targeting 40 randomly-distributed genomic loci, this novel approach greatly reduces bias associated with assays that target single genomic loci.

Duration: 45 minutes followed by Q&A session.

Schedule:

Friday, March 27, 2015 at 11:00 AM Eastern    Status: Available Reserve

Note: Viewers will be asked to register before viewing the previously recorded webinars.