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Online Seminars

SABiosciences is pleased to provide free, on-line educational resources covering relevant tools and techniques for gene and protein expression analysis, and cell-based gene function analysis. We offer live webinars, prerecorded and PDF formatted presentations.

See the instructions for the Web Seminars

February 2017
Monday
Tuesday
Wednesday
Thursday
Friday

1

Isolate exosomes for protein analysis

2

QuantiFERON: TB diagnosis and treatment

3

6

Introduction to miRNA-seq

7

QIAcout for single cell isolation

8

Maximize quantity and purity of exosomal RNA

9

Introduction to miRNA-seq

Cancer research and challenges of FFPE

10

13

14

15

Circulating exosomes and exoRNA detection

NGS sample QC

16

Controls and novel solutions for real-time qPCR

miRNA-seq in liquid biopsy

17

20

QIAcout for single cell isolation

21

Methylome sequencing

Microbial sample prep

22

Exosome profiling by RNA-seq Explorer solution

23

Her2 miracle in CTC and EMT correlation

24

27

Her2 miracle in CTC and EMT correlation

28

Current Seminar Titles Available:

Focus Title
1.  Single cell analysisQIAscout: Overcoming challenges in single cell isolation
2.  RNA-seq Explorer solutionTranscriptome analysis of Kupffer cells after uptake of pancreatic cancer exosomes reveal pathways and biological processes involved in metastatic progression
3.  AdnaTestsThe Her2 miracle in CTCs and its correlation to EMT and tumor stemness
4.  Microbial analysis of human stool sampleSemi-automated low-throughput workflow for microbial analyses of human stool
5.  QIAseq Library PrepMethylome sequencing: a high-performance post-bisulfite library construction (PBLC) protocol

QIAscout: Overcoming challenges in single cell isolation

"Single cell analysis is getting into the focus of many research fields as it demonstrates the individual contribution of every cell in a heterogeneous population without obscuring a biological response that may otherwise occur when cells are assessed in bulk. Although single-cell research is currently gaining momentum, yet it remains challenging due to the lack of affordable methods to precisely isolate a single cell from a heterogeneous cell population without causing high cellular stress. The QIAscout system is an effective and fast method to isolate viable single cells ensuring minimal manipulation of the cellular status. The QIAscout array is ideal for various cell types like adherent cells, suspension cells, cell lines, primary cells, and fluorescent cell lines providing an environment suitable for their growth and viability similar to any standard cell culture dish. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, February 20, 2017 at 9:30 AM Eastern    Status: Available Reserve

Transcriptome analysis of Kupffer cells after uptake of pancreatic cancer exosomes reveal pathways and biological processes involved in metastatic progression

Pancreatic cancer is one of the most lethal malignancies with a poor prognosis. Understanding the mechanisms of tumor progression is therefore essential. Liquid biopsies are non-invasive methods for diagnostics to detect early stage cancer resulting in more successful treatment. One liquid biopsy technique is the detection of RNA from tumor- derived exosomes. These exosomes participate in generating a metastatic niche. Using QIAGEN Bioinformatics solutions with a publicly available dataset we have examined the transcriptome of Kupffer cells after uptake of pancreatic tumor-derived exosomes that induce the formation of a liver metastatic niche and analyzed the pathways and biological processes involved in this process.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, February 22, 2017 at 1:00 PM Eastern    Status: Available Reserve

The Her2 miracle in CTCs and its correlation to EMT and tumor stemness

"Breast cancer is the most common cancer in women, accounting for 23% of all cancer diagnoses. Early detection of breast cancer metastasis would allow for changes in treatment strategy before the disease progression becomes apparent. In this webinar, we will demonstrate the enrichment and detection value as well as the prognostic and predictive value of the AdnaTestBreast in breast cancer. Using the Combination of Combinations Principle (COCP), AdnaTest BreastCancer enables highly specific immunomagnetic cell selection for enriching circulating tumor cells (CTCs) from peripheral blood. It also allows sensitive analysis of breast cancer-associated gene expression in enriched CTCs by reverse transcription and PCR. The high performance of AdnaTest BreastCancer has been demonstrated in dozens of peer-reviewed publications. Several studies have reported that HER2 expression is different in primary tumors compared to CTCs. In addition, AdnaTestBreast HER2 overexpression profile corresponds to HER2 metastatic phenotype; and HER2 detected by the AdnaTestBreast and AdnaTest EMT-2/StemCell seems to initiate EMT and tumor stemness."

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, February 23, 2017 at 1:00 PM Eastern    Status: Available Reserve
Monday, February 27, 2017 at 9:30 AM Eastern    Status: Available Reserve

Semi-automated low-throughput workflow for microbial analyses of human stool

The gut microbiota composition changes dramatically throughout ageing and disease. A healthy gut microbiota is typically characterized by large bacterial taxonomic diversity and functional capacity, whereas frailty and ageing are associated with loss of diversity and expansion of more pathogenic bacterial species. However, in order to accurately profile changes in microbial communities, the reproducible isolation of high quality DNA is an important step. Automation allows for convenient, reliable and reproducible isolation of high quality DNA, which can be used directly for downstream sequencing applications. In this webinar, we will discuss the development of a semi-automated workflow to profile the gut microbiota of young and old individuals and identify changes in bacterial composition and function that occur with age. This workflow will help to simplify and streamline the DNA extraction process for samples with high inhibitor content and subsequent microbial community analyses.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, February 21, 2017 at 1:00 PM Eastern    Status: Available Reserve

Methylome sequencing: a high-performance post-bisulfite library construction (PBLC) protocol

Epigenetic changes, such as the methylation of cytosine, have been identified as key factors in various diseases. They play a crucial role in the regulation of important cellular processes, such as gene expression and cellular differentiation. Here, we describe a post-bisulfite library construction (PBLC) protocol that can overcome challenges frequently encountered with the classic whole genome bisulfite sequencing (WGBS) method, including low library yields and PCR amplification bias. We explore the establishment of a PBLC protocol that uses the combination of the EpiTect Fast Bisulfite Kit for bisulfite conversion of non-methylated cytosines along with the end-polishing and ligation chemistries of the QIAseq Ultralow Input Library Kit.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, February 21, 2017 at 9:30 AM Eastern    Status: Available Reserve

Note: Viewers will be asked to register before viewing the previously recorded webinars.