QIAGEN Website    Quick Order    Online Seminar    Contact    My Account
Bookmark and Share Home  >  Resources  >  Online Seminars

For the most updated product information, visit GeneGlobe on QIAGEN website

Online Seminars

SABiosciences is pleased to provide free, on-line educational resources covering relevant tools and techniques for gene and protein expression analysis, and cell-based gene function analysis. We offer live webinars, prerecorded and PDF formatted presentations.

See the instructions for the Web Seminars

July 2012
Monday
Tuesday
Wednesday
Thursday
Friday

2

PCR Arrays for Pathway Analysis

3

Identify key players and regulators in cancer

4

5

PCR Array Data Analysis Tutorial

6

Functional analysis of genes, biologics & small molecules

9

RNAi Screen Design & Optimzation

10

Epigenetic Technologies for Biomedical Research

11

Innate immune system

QPCR Introduction

12

ChIP and Real-Time PCR Technology Overview

13

Oncogenomics & cancer mutation

16

Cell Death Research Solutions

17

Wnt Signaling Research Solutions

18

Gene Network Central Tutorial

Research tools for inflammation regulation

19

Anticancer Immunity

20

Copy number: variation & alteration analysis strategies

23

Metabolism and cancer

24

Wnt application- a case study

25

Profiling DNA Methylation with PCR System

PCR Array Data Analysis Tutorial

26

RNAi Gene Knockdown with shRNA

27

Oncogenomics & cancer mutation

miRNA Expression and Function

30

Signal transduction targets

31

Cancer and Inflammation

Current Seminar Titles Available:

Focus Title
1.  QIAseq Library PrepMethylome sequencing: a high-performance post-bisulfite library construction (PBLC) protocol
2.  QIAseq miRNA Library KitmiRNA-seq from liquid biopsy: robust detection from the lowest sample amounts
3.  QIAxcel systemIncreasing productivity in Multiplex PCR applications with QIAxcel
4.  QIAseq NGS Library KitQIAseq Technologies for Low Input Whole Genome Sequencing
5.  QuantiFERON-TBAn Update: Breakthrough Research on IGRA Use in Pediatric TB testing

Methylome sequencing: a high-performance post-bisulfite library construction (PBLC) protocol

Epigenetic changes, such as the methylation of cytosine, have been identified as key factors in various diseases. They play a crucial role in the regulation of important cellular processes, such as gene expression and cellular differentiation. Here, we describe a post-bisulfite library construction (PBLC) protocol that can overcome challenges frequently encountered with the classic whole genome bisulfite sequencing (WGBS) method, including low library yields and PCR amplification bias. We explore the establishment of a PBLC protocol that uses the combination of the EpiTect Fast Bisulfite Kit – for bisulfite conversion of non-methylated cytosines – along with the end-polishing and ligation chemistries of the QIAseq Ultralow Input Library Kit.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, April 25, 2017 at 9:30 AM Eastern    Status: Available Reserve

miRNA-seq from liquid biopsy: robust detection from the lowest sample amounts

miRNAs impact virtually all areas of biology, and in circulation, they are promising biomarker candidates for both normal and disease biology. miRNAs are protected from degradation in virtually all biofluids by exosomes, Ago2, HDL or other protective proteins, but are expressed at low levels. As a result, expression analysis, particularly using next-generation sequencing (NGS), has proven to be difficult. Traditional small RNA library kits lack the sensitivity or specificity to adequately assess miRNA expression. Libraries prepared with using these kits have been fraught with background products, such as adapter dimers and other RNAs, including hY4 Y RNA. These problems collectively manifest as a low mapping percentage to miRNA, a limited dynamic range and lost discovery potential. QIAGEN’s QIAseq miRNA Library Kit is specifically designed to overcome these challenges. The innovative, gel-free workflow enables the preparation of robust libraries from even the most difficult, low RNA content biofluids. QIAseq miRNA maximizes your on-target miRNA reads, dynamic range and, mostly importantly, your discovery potential.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, April 27, 2017 at 1:00 PM Eastern    Status: Available Reserve

Increasing productivity in Multiplex PCR applications with QIAxcel

"The most commonly used method for analysis of genotyping assays based on end-point PCR is gel electrophoresis, using manually poured slab gels. This method is extremely labor intensive and exposes users to hazardous chemicals such as ethidium bromide. In addition, resolution of such gels is often poor and detailed analysis of the data in terms of determination of fragment size can be tedious — especially when the data are to be compared with previously analyzed PCR products. This webinar focuses on the challenges of typical genotyping applications and describes possible solutions for these, covering aspects from sample purification to detection of end-point PCR products using the QIAxcel system. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, April 27, 2017 at 9:30 AM Eastern    Status: Available Reserve

QIAseq Technologies for Low Input Whole Genome Sequencing

"Rapidly developing next-generation sequencing (NGS) technologies provide highly sensitive methods in discovering and characterizing the genetic information of a variety of samples. However, DNA samples are often limited in quantity, as well as compromised in quality. Such samples are not suitable for standard NGS library construction methods, which commonly require hundreds of nanograms of good-quality DNA. Examples of such challenging clinical samples include circulating DNA, laser capture microdissection (LCM) samples, formalin-fixed paraffin-embedded (FFPE) samples, ancient DNA and chromatin immunoprecipitation (ChIP) samples. In this webinar, we describe the measures that should be taken into consideration while sequencing such challenging samples. We will also present methods that can be used to optimize library construction to efficiently convert small amounts of DNA samples into sequencing libraries, especially for whole genome sequencing applications. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, April 26, 2017 at 9:30 AM Eastern    Status: Available Reserve

An Update: Breakthrough Research on IGRA Use in Pediatric TB testing

"A number of recent research studies detail the potential use of IGRAs to test for TB infection in pediatric populations. These include a milestone study that indicated, for the first time, a test’s ability to predict a group of children who were more likely to progress to active tuberculosis using QuantiFERON-TB Gold. Join Dr. Islam as he reviews recent studies and current pediatric guidance on IGRAs and discusses the topic, “TST preferred in children under 5: Is there enough data to say otherwise?” Shamim Islam, MD is Clinical Assistant Professor and Attending Physician at the University at Buffalo SUNY. Dr. Islam is a former Pediatric Infectious Disease Physician as well as a former Clinic Physician in San Francisco TB Control Program. The performance of the USA format of the QuantiFERON-TB Gold test has not been extensively evaluated with specimens from individuals younger than age 17 years"

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, April 26, 2017 at 1:00 PM Eastern    Status: Available Reserve

Note: Viewers will be asked to register before viewing the previously recorded webinars.