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Online Seminars

SABiosciences is pleased to provide free, on-line educational resources covering relevant tools and techniques for gene and protein expression analysis, and cell-based gene function analysis. We offer live webinars, prerecorded and PDF formatted presentations.

See the instructions for the Web Seminars

September 2012
Monday
Tuesday
Wednesday
Thursday
Friday

3

4

Anticancer Immunity

PCR Arrays for Pathway Analysis

5

miRNAs in Serum

Functional analysis of genes, biologics & small molecules

6

QPCR Introduction

EMT and ECM

7

RNAi Screen Design & Optimzation

miRNA overview

10

Metabolism and cancer

11

Oncogenomics & cancer mutation

12

Gene Network Central Tutorial

13

PCR Array Data Analysis Tutorial

14

miRNAs expression and quantitation

17

Epigenetic Technologies for Biomedical Research

18

Copy number: variation & alteration analysis strategies

Oxidative Stress Research Solutions

19

Profiling DNA Methylation with PCR System

Innate immune system

20

Drug induced toxicity and metabolism

PCR Arrays for Pathway Analysis

21

RNAi Gene Knockdown with shRNA

miRNAs in Serum

24

Cell Death Research Solutions

25

Fibrosis and wound healing

PCR Array Data Analysis Tutorial

26

ChIP and Real-Time PCR Technology Overview

Research tools for inflammation regulation

27

Discover miRNA, Proteins and Pathways that Regulate Any Gene

Copy number: variation & alteration analysis strategies

28

ELISArray- Analyze Multiple Cytokines and Chemokines

Cancer and Inflammation

Current Seminar Titles Available:

Focus Title
1.  PCR ArrayPCR Array Data Analysis Tutorial
2.  QPCR IntroductionIntroduction to Real Time PCR (Q-PCR/qPCR/qrt-PCR)
3.  miRNAsThe miRNA revolution: An introduction to microRNA biogenesis, function & analysis
4.  miRNAsmiRNA Quantitation: From experimental design through data analysis
5.  PCR Arrays in ImmunologyExploring the first line of defense: research tools for the innate immune system
6.  Inflammation and TLRToll-like Receptors in Inflammation
7.  miRNAsFunctional analysis of miRNAs
8.  Next-generation sequencingNext-generation sequencing, an introduction to technology and applications
9.  Microbial PCR ArraysMicrobiome: From identification to characterization
10.  miRNAsMicroRNA biomarker discovery - overcoming limiting sample material
11.  Host–Pathogen InteractionsHost–Pathogen Interactions: Molecular Basis and Host Defense Mechanisms
12.  Next-generation sequencingNext-Generation Sequencing — Targeted Enrichment Technology in Cancer Research
13.  Next-generation sequencingAdvancing NGS Data Analysis and Interpretation: CLC Cancer Research Workbench and Ingenuity Variant Analysis
14.  Next-generation sequencingAddressing the challenges of NGS workflow: sample preparation, quality control and automation
15.  Single cell analysisSingle cell analysis - from sample to insight
16.  Single cell analysisChallenges and solutions of single cell analysis
17.  Service CoreAccelerate your discovery with QIAGEN service solutions for biomarker research
18.  Multiplex PCRPractical hints and new solutions for successful real-time PCR studies
19.  Multiplex PCRCritical factors for successful multiplex endpoint PCR
20.  Multiplex PCRCritical Factors for Successful Multiplex Real-Time PCR
21.  PCR ArraysAdvanced Real-Time PCR Array Technology – Coding & Noncoding RNA Expression Analysis
22.  QuantiNova PCR kit Novel in-process monitoring tools for improving accuracy and reproducibility of qPCR results
23.  RNA IsolationBack to Basics: Fundamental Concepts and Special Considerations in RNA Isolation
24.  QIAmp circulating NA kitGetting Past the Pitfalls: Best Practices in Pre-analytical Sample Handling for Free Circulating DNA Isolation
25.  Single cell analysisSingle-Cell Mutation Detection – Overcoming Challenges in Single-Cell Analysis
26.  Liquid BiopsiesLiquid biopsy: overcome challenges of circulating DNA with automated and standardized extraction processes
27.  Liquid BiopsiesSensitive mutation detection by sequencing circulating cell-free DNA
28.  AutomationStreamlining the Gene Expression Workflow while Increasing Data Quality
29.  Multiplex PCRMultiplex PCR for Genotyping: Technology Overview and Applications
30.  AutomationGenotyping Workflow: Challenges and Streamlined Solutions with QIAxcel Systems
31.  Liquid BiopsiesExosome Research – New solutions for enrichment and isolation of exosomes and exosomal RNA
32.  Liquid BiopsiesCirculating Tumor Cells: the Seeds of Metastazation
33.  Liquid BiopsiesMolecular Setup of Circulating Tumor Cells Predicts Drug Responsiveness
34.  RNA isolationRNA Integrity and Quality – Standardize RNA Quality Control
35.  RNA isolationChallenges of FFPE Sample Materials – Where Does Variation in Quantity of Purified DNA Come From?

PCR Array Data Analysis Tutorial

With a live demonstration using actual PCR array data, learn how easy it is to use our data analysis Web portal to calculate fold-differences in gene expression from your raw real-time PCR threshold cycles.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, September 17, 2015 at 1:00 PM Eastern    Status: Available Reserve
Monday, October 19, 2015 at 9:30 AM Eastern    Status: Available Reserve

Introduction to Real Time PCR (Q-PCR/qPCR/qrt-PCR)

Real-time polymerase chain reaction (qPCR) is the most sensitive and reliable method for the detection and quantification of nucleic acids. This 45-minute webinar introduces the concepts of real-time PCR and how to conduct a real-time PCR assay. The topics that will be covered include an overview of real-time PCR chemistries, protocols, quantification methods, real-time PCR applications, and factors for success. This webinar is for both beginners that are new to real-time PCR as well as experienced users.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, September 3, 2015 at 1:00 PM Eastern    Status: Available Reserve
Monday, October 5, 2015 at 9:30 AM Eastern    Status: Available Reserve

The miRNA revolution: An introduction to microRNA biogenesis, function & analysis

miRNA plays a critical role in many biological processes such as differentiation and development, cell signaling, response to infection, and other challenges. Recent studies have shown overwhelming evidence that aberration in miRNA expression is causal or an indicator of many disease processes including cancer. Currently there is a race to find and develop miRNAs as biomarkers and therapeutics. To win the race, it is important to have a strong understanding of miRNA and the challenges of miRNA research. The webinar will cover the biology of miRNA, the key challenges associated with miRNA research, and the latest advances in miRNA research technology.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, September 17, 2015 at 9:30 AM Eastern    Status: Available Reserve
Thursday, October 15, 2015 at 1:00 PM Eastern    Status: Available Reserve

miRNA Quantitation: From experimental design through data analysis

miRNAs play a critical role in many biological processes including neuronal cell fate, hematology, apoptosis and developmental biology. Increasing evidence has shown that aberrations in microRNA regulation are associated with many disease processes. This webinar will discuss the challenges associated with miRNA quantification by real-time PCR, and the latest strategies for miRNA quantification and profiling. The critical parameters of profiling miRNA gene expression will also be presented. Come and learn how you can apply these tools in your miRNA research!

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, September 24, 2015 at 9:30 AM Eastern    Status: Available Reserve
Thursday, October 22, 2015 at 1:00 PM Eastern    Status: Available Reserve

Exploring the first line of defense: research tools for the innate immune system

The innate immune system executes crucial and unique functions for host defense against infection. This webinar provides an overview of the most important cellular and molecular players of innate immunity and discusses their functions in a variety of disease states. We also introduce research technologies for exploring innate immune activity in your system through profiling of gene expression and cytokines, signal transduction pathway analysis and more, all in the context.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, October 21, 2015 at 9:30 AM Eastern    Status: Available Reserve

Toll-like Receptors in Inflammation

"Toll-like receptors (TLRs) have been implicated in both innate and adaptive immunity-induced inflammation, thereby playing critical roles in providing the host with short- and long-term protection against infections.. This webinar will provide an overview of the roles that TLRs play in the regulation of Inflammation and solutions for studying these roles. An overview of TLR-mediated inflammation, the key signaling players involved in TLR-mediated inflammation, and the contribution of TLR-mediated inflammation to various physiological processes will be presented. Please come and learn about the state-of-the-art research tools for analyzing TLR-mediated inflammation and its regulation. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, October 28, 2015 at 9:30 AM Eastern    Status: Available Reserve

Functional analysis of miRNAs

Understanding the function and targets of miRNA is important, but challenging research. Since one miRNA can potentially target several genes, finding and validating a specific miRNA-mRNA interaction often involves multiple molecular techniques. In this webinar we will highlight the use of miRNA mimics, inhibitors and target protectors to increase, decrease and adjust the cellular concentration of miRNA and disrupt specific miRNA-mRNA interactions. We will also present a ready-to-use screening tool for identifying miRNA targets in your samples. Lastly, we will show how miRNA expression data can be used to predict mRNA targets and how qPCR can be used to validate predicted interactions.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, October 1, 2015 at 9:30 AM Eastern    Status: Available Reserve
Thursday, October 29, 2015 at 1:00 PM Eastern    Status: Available Reserve

Next-generation sequencing, an introduction to technology and applications

Next-generation sequencing (NGS) technology has revolutionized the study of the genomes, transcriptomes and epigenomes of any species. NGS employs massively parallel sequencing to poduce millions of sequences at once and delivering fast, inexpensive, and accurate genome information. This webinar will provide a technical overview of DNA/RNA preprocessing, template preparation, sequencing, and data analysis. It will also cover the applications for NGS technologies, including guidelines for how to select the technology that will best address your biological question of interest.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, September 8, 2015 at 1:00 PM Eastern    Status: Available Reserve
Tuesday, October 6, 2015 at 9:30 AM Eastern    Status: Available Reserve

Microbiome: From identification to characterization

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, September 1, 2015 at 1:00 PM Eastern    Status: Available Reserve
Wednesday, October 14, 2015 at 9:30 AM Eastern    Status: Available Reserve

MicroRNA biomarker discovery - overcoming limiting sample material

This webinar presents an integrated, PCR-based system that reduces the amount of sample required for full miRNome profiling and provides unparalleled reproducibility and precision. This advance opens up new possibilities for biomarker development.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, September 10, 2015 at 9:30 AM Eastern    Status: Available Reserve
Thursday, October 8, 2015 at 1:00 PM Eastern    Status: Available Reserve

Host–Pathogen Interactions: Molecular Basis and Host Defense Mechanisms

Host–pathogen interactions are strikingly complex during infection. This webinar provides an overview of the molecular basis of these intricate interactions; the impact of microbiota on innate and adaptive immunity, metabolism, and insulin resistance; and host defense mechanisms, including newly reported inflammasome-mediated responses and miRNA-mediated responses. Various research tools will be introduced to simplify and streamline each step of studying the host response, enabling analysis of gene expression and regulation, epigenetic modification, genotyping, and signal transduction pathway activation.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, October 7, 2015 at 9:30 AM Eastern    Status: Available Reserve

Next-Generation Sequencing — Targeted Enrichment Technology in Cancer Research

Genetic variance analysis plays a critical role cancer diagnosis, prognosis and treatment of cancer. Low sample volume and poor DNA quality are nagging issues with cancer samples making the results unreliable and expensive. This webinar discusses the most biologically efficient, cost-effective method for successful NGS. The GeneRead DNA QuantiMIZE Kits enable determination of the optimum conditions for targeted enrichment of DNA isolated from biological samples, while the GeneRead DNAseq Panels V2 allow you to quickly and reliably deep sequence your genes of interest. Applications in translational and clinical research will be highlighted.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, September 22, 2015 at 1:00 PM Eastern    Status: Available Reserve
Tuesday, October 20, 2015 at 9:30 AM Eastern    Status: Available Reserve
Friday, October 23, 2015 at 11:00 AM Eastern    Status: Available Reserve

Advancing NGS Data Analysis and Interpretation: CLC Cancer Research Workbench and Ingenuity Variant Analysis

Deep-sequencing technologies are enabling critical insights into all aspects of cancer genomics. This webinar will discuss two comprehensive informatics solutions — the CLC Cancer Research Workbench and Ingenuity Knowledge Base Variant Analysis platforms. These NGS analysis platforms provide a user-friendly and customizable solution, enabling you to easily narrow down your focus to causal variants and detect and annotate low-frequency somatic variants. We will show the intuitive user interface of CLC Cancer Research Workbench and demonstrate how the rich biological content from Ingenuity Knowledge Base helps you rapidly identify critical variants in your samples.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, September 29, 2015 at 1:00 PM Eastern    Status: Available Reserve
Tuesday, October 27, 2015 at 9:30 AM Eastern    Status: Available Reserve
Friday, October 30, 2015 at 11:00 AM Eastern    Status: Available Reserve

Addressing the challenges of NGS workflow: sample preparation, quality control and automation

The rapid technological advances in NGS have revolutionized the field of genomics, and broaden the applications from basic research, translational to clinical research. However, there exist enormous challenges in terms of sample preparation, sample quality control, and library preparation, which affect sequencing quality. This webinar will focus on these challenges and discuss various solutions to tackle these challenges and maximize your NGS workflow.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, September 15, 2015 at 1:00 PM Eastern    Status: Available Reserve
Tuesday, October 13, 2015 at 9:30 AM Eastern    Status: Available Reserve

Single cell analysis - from sample to insight

What can you do from a single cell? Actually, a lot! Beginning with the genome, you can discover new biomarkers by identifying new genetic variances and their association with specific diseases, including cancers. Moving on to RNA, the recent advances in RNA sequencing technology have made single-cell transcriptomics a possibility. Along with these possibilities come challenges that start from the moment you get the sample to the final step of gaining insights into the cell. This webinar will provide an overview on the multiple steps involved as you move from sample acquisition to analysis and data interpretation in different sample types.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, September 1, 2015 at 9:30 AM Eastern    Status: Available Reserve
Tuesday, October 6, 2015 at 1:00 PM Eastern    Status: Available Reserve

Challenges and solutions of single cell analysis

Cell heterogeneity plays a central role both in normal development and in disease. Single cell genomic analysis is essential to dissect the genotypic and phenotypic variability, but the small amounts of DNA and mRNA present unique challenges. In this webinar, you will learn about current approaches for single cell genome and transcriptome analysis.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, September 8, 2015 at 9:30 AM Eastern    Status: Available Reserve

Accelerate your discovery with QIAGEN service solutions for biomarker research

Development of preclinical biomarkers can have several pitfalls, often requiring a large investment in instruments to process samples, time to develop assays, and specialties in data analysis and biomarker algorithm design. QIAGEN’s life sciences service core can provide researchers with the tools, knowledge and specialties to help develop the next generation of biomarkers for cancer, toxicology and inflammation research. This seminar will highlight QIAGEN’s service capabilities in sample isolation, microarray and NGS-sequencing, qPCR panel, and custom assay development and bioinformatics as we look at the identification of potential biomarkers and gene signatures. We will discuss the applications of QIAGEN Service Core in microRNA discovery for toxicology markers in serum and plasma and in identification of RNA signatures for tumor stratification. Join us and learn how you can accelerate your research with QIAGEN service solutions.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, August 31, 2015 at 9:30 AM Eastern    Status: Available Reserve
Thursday, September 24, 2015 at 1:00 PM Eastern    Status: Available Reserve
Monday, October 26, 2015 at 9:30 AM Eastern    Status: Available Reserve

Practical hints and new solutions for successful real-time PCR studies

In this webinar we will cover the following topics which are critical steps for efficient and precise gene expression studies using real-time PCR technology: - Effect of RNA integrity on real-time PCR results – tips to achieve a true RNA profiling suitable for real-time PCR studies - Improved methods for cDNA synthesis, optimized for real-time PCR - Real-time PCR analysis • Real-time PCR essentials and background information on different quantification strategies • SYBR Green real-time PCR – factors influencing specificity • Introduction to probe technology • New, fast and efficient real-time PCR solutions

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, October 8, 2015 at 9:30 AM Eastern    Status: Available Reserve

Critical factors for successful multiplex endpoint PCR

Multiplex endpoint PCR is a powerful tool for genotyping and many other applications. The ability to amplify and detect several DNA targets in the same reaction offers many benefits. It enables generation of more data out of less sample material, which is especially beneficial in routines with limited and precious sample material. It also offers the opportunity to run internal controls in every reaction, thus increasing the overall reliability of data acquisition. Most importantly of course, switching PCR-based routine lab testing to multiplex PCR offers the opportunity to substantially save on time, materials and cost. QIAGEN’s multiplex PCR chemistry is optimized for reliable amplification of many different templates with high variability in copy numbers. Thus it enables very quick establishment of a new lab routine and instant success for your multiplex PCR strategy. There is a set of critical factors which we recommend to be regarded for planning and performing this kind of PCR. These will be discussed in detail in the webinar. Additionally, our multiplex PCR chemistry has recently been gaining increasing popularity among scientists who are utilizing it for their next-generation sequencing workflows. We will also briefly touch on this topic.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, September 21, 2015 at 1:00 PM Eastern    Status: Available Reserve

Critical Factors for Successful Multiplex Real-Time PCR

Multiplex real-time PCR is a powerful tool for gene expression analysis, viral load monitoring, genotyping, and many other applications. The ability to amplify and detect several genomic DNA, cDNA, or RNA targets in the same reaction offers many benefits: • Conservation of precious samples – more quantification data per sample • Increased throughput – more targets analyzed per run on a cycler • Reliable results – no well-to-well variability due to co-amplification of internal control • Reduced costs – save time and reagents The QuantiFast Multiplex PCR and RT-PCR kits are optimized for reliable amplification of many different templates despite a high variability in abundance. Thus they enable successful amplification of multiple targets on the first attempt without optimization. This webinar explains the principles of the QIAGEN multiplex technologies and shows data demonstrating the exceptional multiplex real-time PCR performance of the QuantiFast Multiplex kits.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, October 22, 2015 at 9:30 AM Eastern    Status: Available Reserve

Advanced Real-Time PCR Array Technology – Coding & Noncoding RNA Expression Analysis

This webinar presents a simple and accurate real-time PCR system for relevant biological pathway- & disease-focused mRNA and long non-coding RNA (lncRNA) expression profiling. Learn about the stringent performance built into the technology to insure its sensitivity, specificity, reproducibility, and reliability. Application examples will be presented.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, September 10, 2015 at 1:00 PM Eastern    Status: Available Reserve
Monday, October 12, 2015 at 9:30 AM Eastern    Status: Available Reserve
Friday, October 16, 2015 at 11:00 AM Eastern    Status: Available Reserve

Novel in-process monitoring tools for improving accuracy and reproducibility of qPCR results

The increasing demand for streamlined, monitored, and ultrafast qPCR procedures requires high-performance, real-time quantitative RT and PCR chemistries. Particularly procedures utilizing generic kits for gene expression analysis should include in-process safety measures to avoid variables and control accuracy of procedures and results. This webinar presents innovative solutions for one-step and two-step RT-PCR that significantly enhance performance and reliability in qRT-PCR. The new QuantiNova kit family offers a combination of various integrated safety features to remove variables and prevent artifacts. Internal Control RNA, removal of genomic DNA, room temperature set up capability for RT-PCR, and a built-in visual pipetting control verify accurate procedures, ensuring reliable gene expression profiling. This webinar explains the principles of the technologies and shows data demonstrating performance in qRT-PCR. Find out how you can verify accurate performance in qRT-PCR and improve your results.

Duration: 45 minutes followed by Q&A session.

Schedule:

Friday, October 9, 2015 at 2:00 PM Eastern    Status: Available Reserve
Thursday, October 15, 2015 at 9:30 AM Eastern    Status: Available Reserve

Back to Basics: Fundamental Concepts and Special Considerations in RNA Isolation

How are your RNA yields? Some sample types present special challenges in RNA purification and analysis. In this webinar, we will discuss and provide tips for the following topics: • The basic methods and challenges of RNA purification • Special considerations for challenging sample types • Isolating miRNA and extracellular RNA • Performing RNA quality checks Join us to learn how you can apply these practical tips to high quality and quantity of RNA.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, September 14, 2015 at 9:30 AM Eastern    Status: Available Reserve
Monday, October 12, 2015 at 1:00 PM Eastern    Status: Available Reserve

Getting Past the Pitfalls: Best Practices in Pre-analytical Sample Handling for Free Circulating DNA Isolation

Isolation and analysis of free circulating DNA has many challenges. Stabilization and processing samples before the actual isolation has a large impact on downstream results. Best practices for handling samples will be discussed during this webinar. In addition, questions such as how much blood should be taken, what are the best protocols to prepare plasma and what kind of controls can be used will be answered.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, September 16, 2015 at 9:30 AM Eastern    Status: Available Reserve
Wednesday, October 14, 2015 at 1:00 PM Eastern    Status: Available Reserve

Single-Cell Mutation Detection – Overcoming Challenges in Single-Cell Analysis

Genetic alteration is the driving factor behind various biological processes such as developmental malformation, evolution and cancer. Recent findings of genomic heterogeneity among supposedly homogeneous cell populations such as tumor cells demands genomic characterization at the individual cell level to better understand the underlying biology. Single nucleotide variation (SNV) analysis by next-generation sequencing (NGS) requires relatively high coverage that often necessitates targeted enrichment or amplification to focus on sites of interest. In this webinar, we will present a multiplex PCR-based targeted enrichment sequencing method for detecting mutations in single cells isolated from two colon cancer cell lines, Lovo and HT29. We will discuss the technologies along the workflow as well as the challenges and how to address them.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, September 15, 2015 at 9:30 AM Eastern    Status: Available Reserve
Tuesday, October 13, 2015 at 1:00 PM Eastern    Status: Available Reserve

Liquid biopsy: overcome challenges of circulating DNA with automated and standardized extraction processes

Circulating, cell-free DNA (cfDNA) originating from malignant tumors, a developing fetus and also from inflammatory tissues, is present in the cell-free nucleic acids in plasma, serum and other body fluids and is considered a “liquid biopsy”. Access to cfDNA for analysis allows for specific detection of certain disease states based on a simple blood sample. Circulating cell-free DNA shows distinctive properties – it is present mostly as shorter fragments of less than 500 bp and the concentration of cfDNA in a plasma or serum sample is low (approximately 1–100 ng/ml) compared to cellular materials and varies considerably between different individuals. Because of their fragmented nature and low concentration, cfDNA presents a particular challenge for efficient extraction / purification and quantification, such as by qPCR. We present data on solutions for the following critical problems concerning the purification of cfDNA for research and molecular diagnostic applications: • Pre-analytical workflow (blood processing) for analyzing cfDNA • Optimization of cfDNA extraction from plasma samples: low target concentrations require efficient cfDNA enrichment from larger sample volumes • Novel automated extraction of cfDNA using the QIAsymphony SP instrument for liquid biopsy diagnostic applications

Duration: 45 minutes followed by Q&A session.

Schedule:

Friday, September 18, 2015 at 2:00 PM Eastern    Status: Available Reserve
Wednesday, September 23, 2015 at 9:30 AM Eastern    Status: Available Reserve
Wednesday, October 21, 2015 at 1:00 PM Eastern    Status: Available Reserve

Sensitive mutation detection by sequencing circulating cell-free DNA

Circulating DNA, the cell-free DNA (cfDNA) found in serum or plasma, has become a powerful tool in non-invasive prenatal testing (NIPT), as well as in cancer liquid biopsy. In cancer testing, it has been shown that the quantity and integrity, as well as the mutation content of the cfDNA in cancer patients may differ from that in healthy controls. Therefore, cfDNA may serve as a biomarker for cancer diagnosis, prognosis and stratification. High-throughput sequence analysis of the cfDNA using next-generation sequencing (NGS) technologies provides a highly sensitive method in detecting and characterizing somatic mutations in cancer samples. However, the cfDNA concentration in serum is normally very low, which makes sequencing library construction challenging. In this webinar, we will describe the technical challenges facing cfDNA sequencing and present an optimized workflow that combines high-efficiency NGS library construction, unbiased library amplification and target enrichment to sensitively and reliably detect mutations in cfDNA samples.

Duration: 45 minutes followed by Q&A session.

Schedule:

Friday, September 25, 2015 at 2:00 PM Eastern    Status: Available Reserve
Wednesday, September 30, 2015 at 9:30 AM Eastern    Status: Available Reserve
Wednesday, October 28, 2015 at 1:00 PM Eastern    Status: Available Reserve

Streamlining the Gene Expression Workflow while Increasing Data Quality

This webinar will focus on overcoming the challenges and optimizing data quality in the gene expression workflow. The importance of efficient cDNA generation for RT-qPCR is a direct result of the input RNA quality and integrity. Implementing RNA QC analysis prior to RT-qPCR is an integral part of streamlining the gene expression workflow. Achieving optimal results and saving time and money by avoiding processing samples of poor quality can be achieved by using the RNA Integrity Score (RIS) on the QIAxcel as a quality control check into your gene expression workflow. After quality RNA is obtained, efficient and precise RT-qPCR is required to ensure optimal gene expression level detection. Optimal RT-qPCR can only be obtained using a real-time PCR instrument with high thermal uniformity in combination with quality reagents. The combination of the Rotor-Gene Q and QIAGEN specialized master mixes provides minimal need for optimization to achieve superior specificity and optimal robustness for gene expression analysis. Utilizing these state of the art technologies, one can ensure a highly streamlined gene expression workflow with optimal data quality.

Duration: 45 minutes followed by Q&A session.

Schedule:

Friday, September 11, 2015 at 11:00 AM Eastern    Status: Available Reserve
Thursday, October 29, 2015 at 9:30 AM Eastern    Status: Available Reserve

Multiplex PCR for Genotyping: Technology Overview and Applications

PCR-based genotyping is widely used in food testing, pharmacogenomics and genetic variation screening. This webinar will describe various applications of PCR-based genotyping assays, with a focus on the applications of multiplex PCR technology. Strategies to improve assay efficiency and sensitivity, reduce turnaround times and simplify workflows will be discussed. We will present various tools to simplify your genotyping process, including sample collection, isolation to assay setup and analysis.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, September 14, 2015 at 1:00 PM Eastern    Status: Available Reserve

Genotyping Workflow: Challenges and Streamlined Solutions with QIAxcel Systems

Genotyping workflows are tedious, time and resource intensive assays often implying a large number of samples. This webinar focuses on the challenges of typical genotyping application and workflows. It will also illustrate how lab automation addresses these challenges, making genotyping workflow seamless in order to shorten time to result, decrease manual errors and standardize results, from sample disruption and gDNA extraction to detection of end-point PCR amplicons or RT-PCR amplification.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, September 28, 2015 at 1:00 PM Eastern    Status: Available Reserve

Exosome Research – New solutions for enrichment and isolation of exosomes and exosomal RNA

Recent years have seen an increased interest in the significance of RNA and other molecules carried by exosomes and other extracellular vesicles (EVs). Specifically, these vesicles may be the key to identifying circulating biomarkers. The major limitation of this strategy is the ability to isolate these exosomes in a standardized manner. Until now, methods for purifying exosomes for RNA isolation have been time-consuming and inconsistent. QIAGEN has developed solutions to help isolate and enrich exosomes and other extracellular vesicles, to allow the detection of low-abundance RNAs that are in circulation. The seminar will describe new solutions for sample preparation and discuss how to maximize quantity and purity of low-abundance fragments.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, September 9, 2015 at 9:30 AM Eastern    Status: Available Reserve
Monday, October 5, 2015 at 1:00 PM Eastern    Status: Available Reserve

Circulating Tumor Cells: the Seeds of Metastazation

Circulating Tumor Cells (CTCs) are widely recognized as a seed for metastazation and have been extensively explored as a prognostic marker in various solid cancers. In addition to the prognostic power of the presence of CTCs, the molecular characterization analysis of these cells has provided important insights into cancer biology. In this webinar, we will discuss the biology and resistance mechanisms of CTC, which include the roles of CTC in epithelial to mesenchymal transition (EMT) and tumor stem cells. We will present recent and ongoing investigations using the AdnaTest CTC Enrichment and Detection System to address the number of opportunities that CTCs provide in clinical diagnostics. The AdnaTest combines an immunomagnetic enrichment of CTCs followed by molecular profiling of the CTCs captured. The AdnaTest technology could demonstrate in many clinical trials and in different tumor entities its prognostic power in primary disease as well as in the metastatic situation. The AdnaTest's molecular profiling allows easy access to phenotype changes like EMT and tumor stemness in CTCs but also comprehensive molecular profiling of therapeutic targets, which makes the setup useful in the context of companion diagnostics.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, September 22, 2015 at 9:30 AM Eastern    Status: Available Reserve
Tuesday, October 20, 2015 at 1:00 PM Eastern    Status: Available Reserve

Molecular Setup of Circulating Tumor Cells Predicts Drug Responsiveness

"One major obstacle for clinical utility of CTCs is the lack of consensus in methodology and therapeutic guidance. In this webinar, we will discuss the molecular pathways involved in tumor growth and metastazation. In addition, we will present three clinical applications of AdnaTest that have provided important predictive information in cancer patients. • AdnaTest ProstateCancer in detection of the AR-V7 androgen receptor variant in castrate resistant prostate cancer (CRPC) patients • AdnaTest OvarianCancer in detection of ERCC1 positive CTCs to predict platinum resistance before starting the first line platinum therapy • AdnaPanel BreastCancer in detection of Her2 positive CTCs in metastatic breast cancer as an indicator for anti-Her2 targeted regimens Join us and learn how you can accelerate your CTC research with the AdnaTest CTC Enrichment and Detection System."

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, September 29, 2015 at 9:30 AM Eastern    Status: Available Reserve
Tuesday, October 27, 2015 at 1:00 PM Eastern    Status: Available Reserve

RNA Integrity and Quality – Standardize RNA Quality Control

RNA integrity and quality are critical to obtain meaningful and reliable downstream data. This webinar discusses the challenges and considerations of handling RNA samples, the need for quality control analysis and common methods for RNA integrity and quality assessment. The QIAxcel Advanced System will be introduced to automate the process of RNA sample integrity analysis and obtain objective quality measurement. Application data will be presented.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, September 21, 2015 at 9:30 AM Eastern    Status: Available Reserve
Monday, October 19, 2015 at 1:00 PM Eastern    Status: Available Reserve

Challenges of FFPE Sample Materials – Where Does Variation in Quantity of Purified DNA Come From?

"In this live webinar, we will discuss variability in quantity and purity of DNA purified from FFPE samples manually or with automated procedures, assessed by different quantification and quality control methods. We've carried out a comprehensive analysis, so register today and see the results! "

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, September 28, 2015 at 9:30 AM Eastern    Status: Available Reserve
Monday, October 26, 2015 at 1:00 PM Eastern    Status: Available Reserve

Note: Viewers will be asked to register before viewing the previously recorded webinars.