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Online Seminars

SABiosciences is pleased to provide free, on-line educational resources covering relevant tools and techniques for gene and protein expression analysis, and cell-based gene function analysis. We offer live webinars, prerecorded and PDF formatted presentations.

See the instructions for the Web Seminars

September 2012
Monday
Tuesday
Wednesday
Thursday
Friday

3

4

Anticancer Immunity

PCR Arrays for Pathway Analysis

5

miRNAs in Serum

Functional analysis of genes, biologics & small molecules

6

QPCR Introduction

EMT and ECM

7

RNAi Screen Design & Optimzation

miRNA overview

10

Metabolism and cancer

11

Oncogenomics & cancer mutation

12

Gene Network Central Tutorial

13

PCR Array Data Analysis Tutorial

14

miRNAs expression and quantitation

17

Epigenetic Technologies for Biomedical Research

18

Copy number: variation & alteration analysis strategies

Oxidative Stress and ROS signaling

19

Profiling DNA Methylation with PCR System

Innate immune system

20

Drug induced toxicity and metabolism

PCR Arrays for Pathway Analysis

21

RNAi Gene Knockdown with shRNA

miRNAs in Serum

24

Cell Death Research Solutions

25

Fibrosis and wound healing

PCR Array Data Analysis Tutorial

26

ChIP and Real-Time PCR Technology Overview

Research tools for inflammation regulation

27

Discover miRNA, Proteins and Pathways that Regulate Any Gene

Copy number: variation & alteration analysis strategies

28

ELISArray- Analyze Multiple Cytokines and Chemokines

Cancer and Inflammation

Current Seminar Titles Available:

Focus Title
1.  QPCR IntroductionIntroduction to Real Time PCR (Q-PCR/qPCR/qrt-PCR)
2.  PCR ArraysAdvanced Real-Time PCR Array Technology – Coding & Noncoding RNA Expression Analysis
3.  RNA IsolationBack to Basics: Fundamental Concepts and Special Considerations in RNA Isolation
4.  DNA sample isolationFundamental Concepts and Special Considerations in gDNA Isolation for Better Insight
5.  QuantiNova PCR kit The importance of controls and novel solutions for successful real-time qPCR
6.  End-point PCRBack to basics of PCR: comprehensive end-point PCR tips and tricks
7.  QIAseq RNAScanAnalyzing fusion genes with next-generation sequencing technology
8.  QIAseq Targeted DNA PanelsDigital DNA-seq Technology - Targeted Enrichment for Cancer Research
9.  QIAseq Library PrepInnovative NGS Library Construction Technology
10.  QIAseq Library PrepAdvanced NGS library prep for challenging samples
11.  miScript miRNA ArraysIntroduction to microRNA
12.  AdnaTestsCTC Detection and molecular characterisation – Challenges and Solutions
13.  QIAGEN ServiceRNA-seq for biomarker discovery
14.  QIAseq NGSApplications of NGS in Oncology: A perspective on targeted sequencing in a clinical setting
15.  Microbiome bioinformaticsClinical Metagenomics for Rapid Detection of Enteric Pathogens and Characterization of the Intestinal Microbiome
16.  ExosomesCharacterization of RNA from Extracellular Vesicles - Challenges and Solutions
17.  RNA-seq Explorer solutionTranscriptome analysis of Kupffer cells after uptake of pancreatic cancer exosomes reveal pathways and biological processes involved in metastatic progression

Introduction to Real Time PCR (Q-PCR/qPCR/qrt-PCR)

Real-time polymerase chain reaction (qPCR) is the most sensitive and reliable method for the detection and quantification of nucleic acids. This 45-minute webinar introduces the concepts of real-time PCR and how to conduct a real-time PCR assay. The topics that will be covered include an overview of real-time PCR chemistries, protocols, quantification methods, real-time PCR applications, and factors for success. This webinar is for both beginners that are new to real-time PCR as well as experienced users.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, December 5, 2016 at 9:30 AM Eastern    Status: Available Reserve

Advanced Real-Time PCR Array Technology – Coding & Noncoding RNA Expression Analysis

This webinar presents a simple and accurate real-time PCR system for relevant biological pathway- & disease-focused mRNA and long non-coding RNA (lncRNA) expression profiling. Learn about the stringent performance built into the technology to insure its sensitivity, specificity, reproducibility, and reliability. Application examples will be presented.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, December 12, 2016 at 9:30 AM Eastern    Status: Available Reserve

Back to Basics: Fundamental Concepts and Special Considerations in RNA Isolation

How are your RNA yields? Some sample types present special challenges in RNA purification and analysis. In this webinar, we will discuss and provide tips for the following topics: • The basic methods and challenges of RNA purification • Special considerations for challenging sample types • Isolating miRNA and extracellular RNA • Performing RNA quality checks Join us to learn how you can apply these practical tips to high quality and quantity of RNA.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, December 6, 2016 at 9:30 AM Eastern    Status: Available Reserve

Fundamental Concepts and Special Considerations in gDNA Isolation for Better Insight

"How are your gDNA yields? Some sample types present special challenges in DNA purification and analysis. This webinar provides tips for a whole range of sample types that require special consideration. We will cover the following topics in detail: • The basic methods and challenges of gDNA purification • Working with DNA: good laboratory practice • Special considerations for challenging sample types • Performing DNA quality checks"

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, December 13, 2016 at 9:30 AM Eastern    Status: Available Reserve

The importance of controls and novel solutions for successful real-time qPCR

"The increasing demand for streamlined, monitored, and ultrafast qPCR procedures requires high-performance, real-time quantitative RT and PCR chemistries. Particularly procedures utilizing generic kits for gene expression analysis should include in-process safety measures to avoid variables and control accuracy of procedures and results. This webinar presents innovative solutions for one-step and two-step RT-PCR that significantly enhance performance and reliability in qRT-PCR. The new QuantiNova kit family offers a combination of various integrated safety features to remove variables and prevent artifacts. Internal Control RNA, removal of genomic DNA, room temperature set up capability for RT-PCR, and a built-in visual pipetting control verify accurate procedures, ensuring reliable gene expression profiling. This webinar explains the principles of the technologies and shows data demonstrating performance in qRT-PCR. Find out how you can verify accurate performance in qRT-PCR and improve your results."

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, December 12, 2016 at 1:00 PM Eastern    Status: Available Reserve

Back to basics of PCR: comprehensive end-point PCR tips and tricks

"Ever struggled with primer problems and annealing conditions? Ever wondered why your PCR didn’t work out quite right? Then join this webinar on end-point PCR Beginner’s Guide with tips and tricks you won’t find in the textbooks. At the end we also provide the link to download the updated PCR guide. Some of the highlights: • New feature on multiplex PCR for genotyping • New solutions for PCR fragments analysis • Extensive FAQs and troubleshooting tips"

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, December 14, 2016 at 1:00 PM Eastern    Status: Available Reserve

Analyzing fusion genes with next-generation sequencing technology

"Fusion genes are hybrid genes formed by the fusion of two separate genes. Translocation, interstitial deletion and chromosomal inversions are some of the genetic events that can lead to the formation of fusion genes. The occurrence of fusion genes and its implications in cancer have already been known, but the emergence of NGS technology – especially RNA sequencing – offers the potential to detect novel gene fusions. You can learn more about fusion genes and applying NGS to detect them at our upcoming webinar, presented by Raed Samara, Ph.D., QIAGEN’s Global Product Manager for NGS technologies. This webinar will cover: 1. Fusion genes: what they are and a historical perspective 2. Fusion gene detection: the current status 3. RNA sequencing vs. digital RNA sequencing 4. How to detect and accurately quantify novel fusion genes in your sample ? "

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, December 14, 2016 at 9:30 AM Eastern    Status: Available Reserve

Digital DNA-seq Technology - Targeted Enrichment for Cancer Research

Targeted DNA sequencing has become a powerful approach by achieving high coverage of the region of interest while keeping the cost of sequencing and complexity of data interpretation manageable. However, existing PCR-based target enrichment approaches introduce errors due to PCR amplification bias and artifacts, which significantly affects quantification accuracy and limit the ability to confidently detect low-frequency DNA variants. This webinar introduces a new digital sequencing approach that is based on the use of unique molecular indices (UMIs) - QIAseq Targeted DNA Panels. With UMIs, each unique DNA molecule is barcoded before any amplification takes place to correct for PCR errors. Detailed workflow and applications in cancer research will be presented. Join us and learn about this exciting novel digital DNAseq technology.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, December 7, 2016 at 9:30 AM Eastern    Status: Available Reserve

Innovative NGS Library Construction Technology

"Next-generation sequencing (NGS) is a driving force for numerous new and exciting applications, including cancer research, stem cell research, metagenomics, population genetics, medical research and single cell analysis. While NGS technology is continuously improving, library preparation remains one of the biggest bottlenecks in the NGS workflow and includes several time-consuming steps that can result in considerable sample loss and the potential to introduce handling errors. Moreover conducting single-cell genomic analysis using NGS methods has traditionally been challenging since the amount of genomic DNA present in a single cell is very limited. In this webinar we will discuss methods for the preparation of high-quality DNA libraries for use on Illumina NGS platforms using a novel streamlined one-step enzymatic fragmentation, end-repair and A-addition protocol. We will discuss complete PCR-free solutions for whole genome and whole transcriptome sequencing from isolated single cells and low amounts of genomic DNA or RNA combined with a time-saving, one-tube library preparation protocol that does not require extra nucleic acids fragmentation and sample cleanup between steps, minimizing starting material loss and cross-contamination risk, thereby increasing the resolution of NGS data."

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, December 8, 2016 at 1:00 PM Eastern    Status: Available Reserve

Advanced NGS library prep for challenging samples

"Rapidly developing next-generation sequencing (NGS) technologies provide highly sensitive methods in discovering and characterizing the genetic information of a variety of samples. However, DNA samples are often limited in quantity, as well as compromised in quality. Such samples are not suitable for standard NGS library construction methods, which commonly require hundreds of nanograms of good-quality DNA. Examples of such challenging clinical samples include circulating DNA, laser capture microdissection (LCM) samples, formalin-fixed paraffin-embedded (FFPE) samples, ancient DNA and chromatin immunoprecipitation (ChIP) samples. In this webinar, we describe the measures that should be taken into consideration while sequencing such challenging samples. We will also present methods that can be used to optimize library construction to efficiently convert small amounts of DNA samples into sequencing libraries. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, December 15, 2016 at 1:00 PM Eastern    Status: Available Reserve

Introduction to microRNA

"microRNA plays a critical role in many biological processes such as differentiation and development, cell signaling, response to infection, and other challenges. Recent studies have shown overwhelming evidence that aberration in microRNA expression is causal or an indicator of many disease processes including cancer. Currently there is a race to find and develop microRNAs as biomarkers and therapeutics. To win the race, it is important to have a strong understanding of microRNA and the challenges of microRNA research. The webinar will cover the biology of microRNA, the key challenges associated with microRNA research, and the latest advances in microRNA research technology."

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, December 6, 2016 at 1:00 PM Eastern    Status: Available Reserve

CTC Detection and molecular characterisation – Challenges and Solutions

Circulating Tumor Cells (CTCs) have been extensively explored as circulating biomarkers in various cancers. Due to their rarity, heterogeneity and stem cell-like properties, detecting and profiling CTCs from blood samples is very challenging. In this webinar, Dr. Siegfried Hauch will introduce the well-known AdnaTests, which uses the Combination of Combinations Principle (COCP) to enable enriching and detecting CTCs in whole blood with high specificity and sensitivity, and how to overcome challenges in CTC enrichment and detection. The AdnaTests combine an immunomagnetic capturing method that increases purity, and is followed by molecular profiling of the captured CTCs. In addition, leukocyte contamination is another issue in CTCs detection and may lead to false positive results due to illegitimate expression of target genes or false interpretation. The AdnaWash is developed to reduce leukocyte contamination to such a level that whole gene panels can be analyzed while maintaining the required specificity and sensitivity.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, December 15, 2016 at 9:30 AM Eastern    Status: Available Reserve

RNA-seq for biomarker discovery

"A biomarker is a characteristic that can be measured and evaluated as an indicator of a normal biological process, pathological process or response to therapy. Biomarkers can either be DNA- or RNA- based, or even both. Recently, transcriptional biomarkers, which are based on gene expression analysis, are gaining greater importance in clinical research. Transcriptional biomarkers provide information on the latest physiological status of the microenvironment and the response of cells to external or internal factors. The development of RNA sequencing, digital RNA sequencing and new bioinformatics tools enables us to identify, confirm and validate transcriptional (both mRNA and miRNA) biomarkers, , with a high level of accuracy. Limited sample amount, low-quality sample and large sample numbers are some of the common challenges faced by labs undertaking biomarker discovery. In addition, tight timelines and the lack of in-house expertise and facilities also hamper the use of the latest technologies. In this webinar, we will give you an overview of applying RNA sequencing strategies and techniques to biomarker discovery. The webinar will also discuss when to use whole transcriptome or mRNA sequencing and the application of bioinformatics solutions such as CLC Genomics Workbench and Ingenuity Variant Analysis in biomarker discovery. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, December 7, 2016 at 1:00 PM Eastern    Status: Available Reserve

Applications of NGS in Oncology: A perspective on targeted sequencing in a clinical setting

For the past 20 years, the continuing advancement of NGS technologies and data analysis tools has broadened our understanding of cancer and human health immensely. In spite of their widely-acknowledged utility, the transfer of cutting-edge NGS technologies from research settings into translational and ultimately clinical labs is not trivial. This seminar discusses the adoption of targeted sequencing in a clinical setting, including the use of gene panels for studying sequence variants, copy-number variation, and mutations in the mitochondrial genome. Considerations in sample and library preparation, sequencing, data analysis and interpretation will be discussed, with a particular focus on the implications of these data for patients.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, December 7, 2016 at 11:00 AM Eastern    Status: Available Reserve

Clinical Metagenomics for Rapid Detection of Enteric Pathogens and Characterization of the Intestinal Microbiome

High-throughput sequencing, combined with high-resolution metagenomic analysis, provides a powerful diagnostic tool for clinical management of enteric disease. Forty-five patient samples of known and unknown disease etiology and 20 samples from health individuals were subjected to next-generation sequencing. Subsequent metagenomic analysis identified all microorganisms (bacteria, viruses, fungi and parasites) in the samples, including the expected pathogens in the samples of known etiology. Multiple pathogens were detected in the individual samples, providing evidence for polymicrobial infection. Patients were clearly differentiated from healthy individuals based on microorganism abundance and diversity. The speed, accuracy and actionable features of CosmosID bioinformatics and curated GenBook® databases, implemented in the QIAGEN Microbial Genomics Pro Suite, and the functional analysis, leveraging the QIAGEN functional metagenomics workflow, provide a powerful tool contributing to the revolution in clinical diagnostics, prophylactics and therapeutics that is now in progress globally.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, December 8, 2016 at 9:30 AM Eastern    Status: Available Reserve

Characterization of RNA from Extracellular Vesicles - Challenges and Solutions

Exosomes and other extracellular vesicles (EVs) such as microvesicles carry functional cargo and play an important role in disease progression. Recently exosomal RNAs, especially microRNAs, have attracted tremendous interest as potential circulating diagnostic biomarkers. This webinar presents an integrated system for identification and characterization of specific RNA molecules from exosomes and other extracellular vesicles (EVs). Advanced assay technologies for characterization of the RNA profiles including mRNA, microRNA and long non-coding RNAs (RNAs) from serum exosomes will be introduced and discussed.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, December 5, 2016 at 1:00 PM Eastern    Status: Available Reserve

Transcriptome analysis of Kupffer cells after uptake of pancreatic cancer exosomes reveal pathways and biological processes involved in metastatic progression

Pancreatic cancer is one of the most lethal malignancies with a poor prognosis. Understanding the mechanisms of tumor progression is therefore essential. Liquid biopsies are non-invasive methods for diagnostics to detect early stage cancer resulting in more successful treatment. One liquid biopsy technique is the detection of RNA from tumor- derived exosomes. These exosomes participate in generating a metastatic niche. Using QIAGEN Bioinformatics solutions with a publicly available dataset we have examined the transcriptome of Kupffer cells after uptake of pancreatic tumor-derived exosomes that induce the formation of a liver metastatic niche and analyzed the pathways and biological processes involved in this process.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, December 13, 2016 at 1:00 PM Eastern    Status: Available Reserve

Note: Viewers will be asked to register before viewing the previously recorded webinars.