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Online Seminars

SABiosciences is pleased to provide free, on-line educational resources covering relevant tools and techniques for gene and protein expression analysis, and cell-based gene function analysis. We offer live webinars, prerecorded and PDF formatted presentations.

See the instructions for the Web Seminars

September 2012
Monday
Tuesday
Wednesday
Thursday
Friday

3

4

Anticancer Immunity

PCR Arrays for Pathway Analysis

5

miRNAs in Serum

Functional analysis of genes, biologics & small molecules

6

QPCR Introduction

EMT and ECM

7

RNAi Screen Design & Optimzation

miRNA overview

10

Metabolism and cancer

11

Oncogenomics & cancer mutation

12

Gene Network Central Tutorial

13

PCR Array Data Analysis Tutorial

14

miRNAs expression and quantitation

17

Epigenetic Technologies for Biomedical Research

18

Copy number: variation & alteration analysis strategies

Oxidative Stress and ROS signaling

19

Profiling DNA Methylation with PCR System

Innate immune system

20

Drug induced toxicity and metabolism

PCR Arrays for Pathway Analysis

21

RNAi Gene Knockdown with shRNA

miRNAs in Serum

24

Cell Death Research Solutions

25

Fibrosis and wound healing

PCR Array Data Analysis Tutorial

26

ChIP and Real-Time PCR Technology Overview

Research tools for inflammation regulation

27

Discover miRNA, Proteins and Pathways that Regulate Any Gene

Copy number: variation & alteration analysis strategies

28

ELISArray- Analyze Multiple Cytokines and Chemokines

Cancer and Inflammation

Current Seminar Titles Available:

Focus Title
1.  QPCR IntroductionIntroduction to Real Time PCR (Q-PCR/qPCR/qrt-PCR)
2.  PCR Arrays in ImmunologyExploring the first line of defense: research tools for the innate immune system
3.  Next-generation sequencingNext-generation sequencing, an introduction to technology and applications
4.  Microbial PCR ArraysMicrobiome: From identification to characterization
5.  Multiplex PCRPractical hints and new solutions for successful real-time PCR studies
6.  RNA IsolationBack to Basics: Fundamental Concepts and Special Considerations in RNA Isolation
7.  RNA isolationRNA Integrity and Quality – Standardize RNA Quality Control
8.  DNA sample isolationTips and Tricks for Rapid Isolation of Genomic DNA from Solid Tissue Samples
9.  RNAseq technologyDigital RNAseq for gene expression profiling: Workflow and applications
10.  QuantiNova PCR kit The importance of controls and novel solutions for successful real-time qPCR
11.  Liquid BiopsiesNew Technology and workflow for integrated collection, stabilization, and purification of circulating cell-free DNA
12.  Liquid BiopsiesAnalysis and interpretation of cell free DNA
13.  Ingenuity Pathway Analysis (IPA) Interpret Your Gene Expression Analysis Results with Advanced Bioinformatics Tools
14.  Adaptive immunityStudy the Adaptive Immune Response: Tools for T and B cell Research
15.  Liquid BiopsiesLiquid biopsy: overcome challenges of circulating DNA with automated and standardized extraction processes
16.  Multiplex PCR Multiplex End-Point PCR for Genotyping: Critical factors and applications
17.  Pyrosequencing
18.  EpiTect Fast Bisulfite KitsAccurate DNA methylation analysis with successful bisulfite conversion
19.  RNA Quality ControlRNA quality control for accurate results - Interpret your gene-expression results with confidence
20.  PAXgene Tissue PAXgene Tissue – New workflows and applications
21.  Next-generation sequencingNew and highly integrated tools for sensitive next-generation sequencing: Analysis of circulating cell-free DNA
22.  Single cell analysisSingle cell technology introduction
23.  Microbial PCR ArraysProfiling Hospital-Acquired Pathogens and Antibiotic Resistance Genes
24.  QIAxcel systemImprove the reproducibility in your lab by standardizing your sample quality
25.  Microbial DNA IsolationNucleic acid isolation from PCR inhibitor-rich sample types
26.  Liquid BiopsiesCirculating Tumor Cells (CTC) Technology overview, advantages and challenges
27.  EpigeneticsEpigenetics Introduction to research methods with focus on DNA methylation techniques
28.  QIAseq RNAScanAnalyzing fusion genes with next-generation sequencing technology

Introduction to Real Time PCR (Q-PCR/qPCR/qrt-PCR)

Real-time polymerase chain reaction (qPCR) is the most sensitive and reliable method for the detection and quantification of nucleic acids. This 45-minute webinar introduces the concepts of real-time PCR and how to conduct a real-time PCR assay. The topics that will be covered include an overview of real-time PCR chemistries, protocols, quantification methods, real-time PCR applications, and factors for success. This webinar is for both beginners that are new to real-time PCR as well as experienced users.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, August 29, 2016 at 9:30 AM Eastern    Status: Available Reserve

Exploring the first line of defense: research tools for the innate immune system

The innate immune system executes crucial and unique functions for host defense against infection. This webinar provides an overview of the most important cellular and molecular players of innate immunity and discusses their functions in a variety of disease states. We also introduce research technologies for exploring innate immune activity in your system through profiling of gene expression and cytokines, signal transduction pathway analysis and more, all in the context.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, August 23, 2016 at 9:30 AM Eastern    Status: Available Reserve

Next-generation sequencing, an introduction to technology and applications

Next-generation sequencing (NGS) technology has revolutionized the study of the genomes, transcriptomes and epigenomes of any species. NGS employs massively parallel sequencing to poduce millions of sequences at once and delivering fast, inexpensive, and accurate genome information. This webinar will provide a technical overview of DNA/RNA preprocessing, template preparation, sequencing, and data analysis. It will also cover the applications for NGS technologies, including guidelines for how to select the technology that will best address your biological question of interest.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, August 3, 2016 at 9:30 AM Eastern    Status: Available Reserve

Microbiome: From identification to characterization

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, August 8, 2016 at 1:00 PM Eastern    Status: Available Reserve

Practical hints and new solutions for successful real-time PCR studies

In this webinar we will cover the following topics which are critical steps for efficient and precise gene expression studies using real-time PCR technology: - Effect of RNA integrity on real-time PCR results – tips to achieve a true RNA profiling suitable for real-time PCR studies - Improved methods for cDNA synthesis, optimized for real-time PCR - Real-time PCR analysis • Real-time PCR essentials and background information on different quantification strategies • SYBR Green real-time PCR – factors influencing specificity • Introduction to probe technology • New, fast and efficient real-time PCR solutions

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, August 16, 2016 at 1:00 PM Eastern    Status: Available Reserve

Back to Basics: Fundamental Concepts and Special Considerations in RNA Isolation

How are your RNA yields? Some sample types present special challenges in RNA purification and analysis. In this webinar, we will discuss and provide tips for the following topics: • The basic methods and challenges of RNA purification • Special considerations for challenging sample types • Isolating miRNA and extracellular RNA • Performing RNA quality checks Join us to learn how you can apply these practical tips to high quality and quantity of RNA.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, August 17, 2016 at 9:30 AM Eastern    Status: Available Reserve

RNA Integrity and Quality – Standardize RNA Quality Control

RNA integrity and quality are critical to obtain meaningful and reliable downstream data. This webinar discusses the challenges and considerations of handling RNA samples, the need for quality control analysis and common methods for RNA integrity and quality assessment. The QIAxcel Advanced System will be introduced to automate the process of RNA sample integrity analysis and obtain objective quality measurement. Application data will be presented.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, August 31, 2016 at 9:30 AM Eastern    Status: Available Reserve

Tips and Tricks for Rapid Isolation of Genomic DNA from Solid Tissue Samples

Obtaining reliable results from genotyping or sequencing experiments using solid tissue is not always easy. In order to have a successful experiment, you must start with sufficient amounts of high-quality DNA. An inefficient lysis results in poor yields, low DNA integrity and consequently, inferior quality results, thereby limiting the insights you can attain from your sample. In this webinar, we will talk about solutions to overcome the challenges of lysis of fibrous or hard tissue so that you can achieve the insights you value.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, August 8, 2016 at 9:30 AM Eastern    Status: Available Reserve

Digital RNAseq for gene expression profiling: Workflow and applications

Traditional RNA sequencing (RNA-Seq) is a powerful tool for expression profiling, but is hindered by PCR amplification bias and inaccuracy at low expressing genes. QIAseq RNA is a flexible and precise tool developed for mitigating these complications, allowing digital gene expression analysis. This in-depth webinar will cover sample requirements, experimental design, NGS platform-specific challenges and workflow for gene enrichment, library prep and sequencing. The applications of QIASeq RNA Panels in cancer research, stem cell differentiation and elucidating the effects small molecules on signaling pathways will be highlighted.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, August 10, 2016 at 1:00 PM Eastern    Status: Available Reserve

The importance of controls and novel solutions for successful real-time qPCR

"The increasing demand for streamlined, monitored, and ultrafast qPCR procedures requires high-performance, real-time quantitative RT and PCR chemistries. Particularly procedures utilizing generic kits for gene expression analysis should include in-process safety measures to avoid variables and control accuracy of procedures and results. This webinar presents innovative solutions for one-step and two-step RT-PCR that significantly enhance performance and reliability in qRT-PCR. The new QuantiNova kit family offers a combination of various integrated safety features to remove variables and prevent artifacts. Internal Control RNA, removal of genomic DNA, room temperature set up capability for RT-PCR, and a built-in visual pipetting control verify accurate procedures, ensuring reliable gene expression profiling. This webinar explains the principles of the technologies and shows data demonstrating performance in qRT-PCR. Find out how you can verify accurate performance in qRT-PCR and improve your results."

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, August 23, 2016 at 1:00 PM Eastern    Status: Available Reserve

New Technology and workflow for integrated collection, stabilization, and purification of circulating cell-free DNA

Research into non-invasive prenatal testing (NIPT) and circulating tumor DNA (ctDNA) testing based on circulating cell-free DNA (ccfDNA) is rapidly expanding. However, detection and quantification of ccfDNA is compromised by the release of genomic DNA (gDNA) from lymphocytes due to mechanical lysis or apoptosis during blood collection, storage and transport. PreAnalytiX has developed the PAXgene® Blood ccfDNA System, consisting of the PAXgene Blood ccfDNA Tube, a plastic blood collection tube with a unique, non-crosslinking chemistry that preserves extracellular levels of ccfDNA and prevents the release of intracellular DNA from cells into the plasma, and the QIAsymphony® PAXgene Blood ccfDNA Kit for automated ccfDNA extraction from up to 5 ml of plasma. In this webinar, this new technology development will be presented in comparison to other existing technologies.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, August 11, 2016 at 1:00 PM Eastern    Status: Available Reserve

Analysis and interpretation of cell free DNA

Identification and monitoring of cancer mutations from cell free DNA-Seq data is a key application in liquid biopsy. In this part of the webinar we will show how mutations can be best identified from this type of data and how they can be interpreted. Furthermore, potential challenges when analyzing this type of data will be discussed together with relevant strategies.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, August 22, 2016 at 1:00 PM Eastern    Status: Available Reserve

Interpret Your Gene Expression Analysis Results with Advanced Bioinformatics Tools

"You’ve got raw Ct data and fold changes from your gene expression analysis experiment. How do you make sense of the results to build a model that explains the biology and helps you design the next best experiment? In this webinar, we will introduce a gene expression workflow and an easy-to-use yet advanced Ingenuity Pathway Analysis (IPA) bioinformatics tool. With a case study, we will explore the potential that RT-PCR array and bioinformatics combined to help understanding the regulatory roles of some genes in diseases and get better insight into the biological function and find potential biomarkers. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, August 30, 2016 at 1:00 PM Eastern    Status: Available Reserve

Study the Adaptive Immune Response: Tools for T and B cell Research

Adaptive immunity, powered by T cells and B cells, provides specific, long-lasting protection of the host from harmful invaders. This webinar will provide an overview of T cells and B cells and their role in cell-mediated immune responses and antibody responses, respectively, against pathogens. We will explore tools that enable analysis of T and B cell gene expression and regulation, genotyping and signal transduction pathway activation.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, July 28, 2016 at 1:00 PM Eastern    Status: Available Reserve
Tuesday, August 30, 2016 at 9:30 AM Eastern    Status: Available Reserve

Liquid biopsy: overcome challenges of circulating DNA with automated and standardized extraction processes

"Circulating, cell-free DNA (cfDNA) originating from malignant tumors, a developing fetus and also from inflammatory tissues, is present in the cell-free nucleic acids in plasma, serum and other body fluids and is considered a “liquid biopsy”. Access to cfDNA for analysis allows for specific detection of certain disease states based on a simple blood sample. Circulating cell-free DNA shows distinctive properties – it is present mostly as shorter fragments of less than 500 bp and the concentration of cfDNA in a plasma or serum sample is low (approximately 1–100 ng/ml) compared to cellular materials and varies considerably between different individuals. Because of their fragmented nature and low concentration, cfDNA presents a particular challenge for efficient extraction / purification and quantification, such as by qPCR. We present data on solutions for the following critical problems concerning the purification of cfDNA for research and molecular diagnostic applications: • Pre-analytical workflow (blood processing) for analyzing cfDNA • Optimization of cfDNA extraction from plasma samples: low target concentrations require efficient cfDNA enrichment from larger sample volumes • Novel automated extraction of cfDNA using the QIAsymphony SP instrument for liquid biopsy diagnostic applications "

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, August 18, 2016 at 1:00 PM Eastern    Status: Available Reserve

Multiplex End-Point PCR for Genotyping: Critical factors and applications

"Multiplex endpoint PCR is a powerful tool for genotyping and many other applications. The ability to amplify and detect several DNA targets in the same reaction offers many benefits. It enables generation of more data out of less sample material, which is especially beneficial in routines with limited and precious sample material. It also offers the opportunity to run internal controls in every reaction, thus increasing the overall reliability of data acquisition. Most importantly, switching PCR-based routine lab testing to multiplex PCR allows you to save time, materials and cost. This webinar discusses the critical factors in planning and performing multiplex PCR. We will describe an optimized multiplex PCR chemistry for reliable amplification of multiple templates with high variability in copy numbers. Come and learn how QIAGEN's multiplex PCR chemistry allows you to easily set up multiplex PCR. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, July 26, 2016 at 1:00 PM Eastern    Status: Available Reserve
Monday, August 22, 2016 at 9:30 AM Eastern    Status: Available Reserve

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, August 25, 2016 at 9:30 AM Eastern    Status: Available Reserve

Accurate DNA methylation analysis with successful bisulfite conversion

"Bisulfite conversion is a popular used method for DNA methylation analysis. It is the most convenient and effective way to map DNA methylation to individual bases. The efficiency of bisulfite conversion has a huge impact on the reliability of downstream analysis methods and complete conversion is a prerequisite for the correct determination of methylation. However, standard methods to achieve complete conversion require harsh conditions with long incubation times at high temperatures. This harsh treatment can lead to DNA degradation, lowering the yields and sensitivity of the subsequent analysis. This webinar will: • Explain the principle of bisulfite conversion • Point out the challenges and critical factors for successful bisulfite conversion • Describe how to overcome the challenges with QIAGEN’s EpiTect Fast Bisulfite Kits • Give general recommendations for planning successful bisulfite conversion experiments "

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, August 18, 2016 at 9:30 AM Eastern    Status: Available Reserve

RNA quality control for accurate results - Interpret your gene-expression results with confidence

"Quality results can only be achieved from quality samples. By their very nature, RNA molecules, especially mRNA and regulator RNA, are labile and can be highly unstable and sensitive to heat, UV and contamination. The final results’ quality directly depends on the ability to extract RNA without losing any fraction of interest while preserving the integrity of the biological information it carries. RNA quality control is thus critical to ensure quality results and turning these results into actionable insights with confidence. In this webinar, Dr. Pierre-Henri Ferdinand will review the different QC steps along the gene-expression workflow, their associated challenges and relevance for downstream applications. He will introduce technical solutions to track the quality of your RNA samples along your workflow so you can analyse and interpret your gene-expression results with confidence."

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, August 24, 2016 at 9:30 AM Eastern    Status: Available Reserve

PAXgene Tissue – New workflows and applications

The PAXgene Tissue product line is a formaldehyde-free solution for collection, stabilization and purification of tissue specimens for simultaneous preservation of biomolecules and histomorphology. This webinar will introduce a complete portfolio that will focus on new workflows and applications. In particular, we will present the generation of fixed and cryo-embedded (PFCE) tissue blocks using the PAXgene Tissue product line, along with a comparison of histological features and purification of biomolecules (DNA, RNA, miRNA, proteins). Also, Laser-Microdissection (LMD) of PAXgene Tissue fixed and stabilized samples for isolation of DNA and RNA will be addressed as a new application for PAXgene Tissue. These new workflows and protocols complete PreAnalytiX’s and QIAGEN’s unique solutions for formaldehyde-free processing and stabilization of tissue specimens.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, August 1, 2016 at 9:30 AM Eastern    Status: Available Reserve

New and highly integrated tools for sensitive next-generation sequencing: Analysis of circulating cell-free DNA

"Next-generation sequencing of free circulating DNA from plasma, serum and urine has been quickly adopted for cancer testing as well as for noninvasive prenatal testing (NIPT). For cancer patients, this method enables noninvasive diagnoses of actionable mutants to personalize anti-tumor therapies. In addition, this approach could be used to monitor molecular changes during cancer treatment or tumor progression. The concentration of free circulating DNA in liquid biopsies is usually very low. This highlights the critical need to use optimized protocols for cfDNA extraction to construct NGS DNA libraries. In this webinar, we will introduce new technical solutions for Ion Torrent as well as Illumina sequencing platforms that combine optimized cfDNA isolation, highly advanced library construction, unbiased library amplification and integrated data analysis to reliably analyze cfDNA samples with high sensitivity. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, August 25, 2016 at 1:00 PM Eastern    Status: Available Reserve

Single cell technology introduction

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, August 24, 2016 at 1:00 PM Eastern    Status: Available Reserve

Profiling Hospital-Acquired Pathogens and Antibiotic Resistance Genes

Hospital-acquired infections (HAIs) are caused by bacterial, viral and fungal pathogens that easily spread through the body. The most common HAIs include urinary tract infections, bloodstream infections and pneumonia. HAIs are becoming more virulent and more resistant to the antibiotics typically used to fight them, making antibiotic resistance a serious public health concern. In this webinar, we will provide an overview of hospital-acquired pathogens and antibiotic resistance. We will also present tools to help you identify and characterize hospital-acquired bacterial species and antibiotic resistance genes in your research samples.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, August 15, 2016 at 1:00 PM Eastern    Status: Available Reserve

Improve the reproducibility in your lab by standardizing your sample quality

"Build-up trust into your DNA samples, save time, money, peace of mind and ensure reproducible results by implementing appropriate Quality Control in your lab. A recently conducted survey by Nature asked over 1500 scientists for their opinion on the state of reproducibility in research. Strikingly, the survey reveals that more than 70% of the researchers have attempted but failed to reproduce another scientist's experiments, and more than half even admitted having failed to reproduce their own experiments. By their very nature, large nucleic acid molecules such as gDNA can be unstable and damaged by heat, UV, contamination with DNase but also formaldehyde of FFPE samples thus jeopardizing the quality of the results and the reproducibility of the experiments. The final results’ quality directly depends on the ability to extract DNA without losing any fraction of interest while preserving the integrity of the biological information it carries. gDNA quality control is thus critical to ensure quality results and turning these results into actionable insights with confidence. In this webinar, Dr. Pierre-Henri Ferdinand will review the different QC steps, their associated challenges and relevance for downstream applications. He will introduce technical solutions to track the quality of your samples along your workflow so you can analyse and interpret your results with confidence."

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, August 16, 2016 at 9:30 AM Eastern    Status: Available Reserve

Nucleic acid isolation from PCR inhibitor-rich sample types

An accurate molecular analysis of microbial constituents within a particular community is contingent upon nucleic acid extraction methodologies that are non-biased and have high-yield. Only by ensuring that all species and classes of microorganisms present in a sample are effectively lysed during extraction will one be able to reliably assess the composition of that sample. An additional challenge faced in nucleic acid extraction is the presence of persistent, co-purifying polymerase inhibitors endogenous to one’s sample. This presentation will focus on nucleic acid extraction tools developed by MO BIO Laboratories that facilitate accurate non-biased community analysis and eliminate common amplification problems via the depletion of endogenous polymerase inhibitors using our patented Inhibitor Removal Technology.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, August 1, 2016 at 1:00 PM Eastern    Status: Available Reserve

Circulating Tumor Cells (CTC) Technology overview, advantages and challenges

CTCs, or circulating tumor cells, are present in the blood of cancer patients. This provides investigators an ideal source of non-invasive samples to better understand cancer. However, studying rare cells in these samples have key challenges, namely their detection and isolation. In this webinar, we will provide an overview of the different platforms on the market, their strategies in how they are used and finally, we will discuss the advantages and disadvantages of each system.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, August 9, 2016 at 1:00 PM Eastern    Status: Available Reserve

Epigenetics Introduction to research methods with focus on DNA methylation techniques

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, August 11, 2016 at 9:30 AM Eastern    Status: Available Reserve

Analyzing fusion genes with next-generation sequencing technology

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, August 17, 2016 at 1:00 PM Eastern    Status: Available Reserve

Note: Viewers will be asked to register before viewing the previously recorded webinars.