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Online Seminars

SABiosciences is pleased to provide free, on-line educational resources covering relevant tools and techniques for gene and protein expression analysis, and cell-based gene function analysis. We offer live webinars, prerecorded and PDF formatted presentations.

See the instructions for the Web Seminars

December 2016
Monday
Tuesday
Wednesday
Thursday
Friday

1

Introduction to NGS

Tools for microbial detection and host-response analysis

2

5

QPCR Introduction

Circulating exosomes and exoRNA detection

6

Basics of sample prep in RNA research

Introduction to microRNA

7

Digital DNA-seq for cancer research

RNA-seq for biomarker discovery

NGS applications in oncology

8

Innovative NGS Library Construction Technology

Microbiome bioinformatics

9

12

Coding & noncoding RNA analysis using qPCR

Controls and novel solutions for real-time qPCR

13

gDNA collection, storage, and purification

Exosome profiling by RNA-seq Explorer solution

14

Analyzing fusion genes with NGS

Tips and tricks for end-point PCR

15

Advanced NGS library prep for challenging samples

CTC detection and characterization

16

19

20

21

22

23

26

27

28

29

30

Current Seminar Titles Available:

Focus Title
1.  QPCR IntroductionIntroduction to Real Time PCR (Q-PCR/qPCR/qrt-PCR)
2.  Liquid BiopsiesExosome Research – Maximize Quantity and Purity of Exosomal RNA
3.  Multiplex PCRCritical Factors for Successful Multiplex Real-Time PCR
4.  QuantiNova PCR kit The importance of controls and novel solutions for successful real-time qPCR
5.  miScript PCR ArraysNoncoding RNAs in cardiovascular disease – potential as biomarkers and more
6.  Microbial DNA PCR ArraysStudying the microbiome: tools for microbial detection and host-response analysis
7.  Liquid BiopsiesExosome Research - Roles of Exosomes in Human Disease and Advanced Isolation Tools
8.  ExosomesCharacterization of RNA from Extracellular Vesicles - Challenges and Solutions
9.  RNA-seq Explorer solutionTranscriptome analysis of Kupffer cells after uptake of pancreatic cancer exosomes reveal pathways and biological processes involved in metastatic progression
10.  QIAseq NGSDigital sequencing technology in oncology: introduction and applications
11.  QIAseq miRNA Library KitIntroduction to miRNA-seq using unique molecular indices and gel-free library construction
12.  Microbial analysis of human stool sampleSemi-automated low-throughput workflow for microbial analyses of human stool
13.  QIAseq Library PrepMethylome sequencing: a high-performance post-bisulfite library construction (PBLC) protocol
14.  QIAseq miRNA Library KitmiRNA-seq from liquid biopsy: robust detection from the lowest sample amounts
15.  CLC Genomics WorkbenchUsing Workflows to perform integrated analysis of mutational pattern and expression in RNA-seq data
16.  BioinformaticsIntroduction to HGMD Professional
17.  QIAxcel systemIncreasing productivity in Multiplex PCR applications with QIAxcel
18.  Microbial Genomics Pro SuiteTaxonomic profiling using shotgun metagenome data
19.  QIAscoutQIAscout applications in identification of subpopulation-specific variants

Introduction to Real Time PCR (Q-PCR/qPCR/qrt-PCR)

Real-time polymerase chain reaction (qPCR) is the most sensitive and reliable method for the detection and quantification of nucleic acids. This 45-minute webinar introduces the concepts of real-time PCR and how to conduct a real-time PCR assay. The topics that will be covered include an overview of real-time PCR chemistries, protocols, quantification methods, real-time PCR applications, and factors for success. This webinar is for both beginners that are new to real-time PCR as well as experienced users.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, April 11, 2017 at 1:00 PM Eastern    Status: Available Reserve

Exosome Research – Maximize Quantity and Purity of Exosomal RNA

Recent years have seen an increased interest in the significance of RNA and other molecules carried by exosomes and other extracellular vesicles (EVs). Specifically, these vesicles may be the key to identifying circulating biomarkers. The major limitation of this strategy is the ability to isolate these exosomes in a standardized manner. Until now, methods for purifying exosomes for RNA isolation have been time-consuming and inconsistent. QIAGEN has developed solutions to help isolate and enrich exosomes and other extracellular vesicles, to allow the detection of low-abundance RNAs that are in circulation. The seminar will describe new solutions for sample preparation and discuss how to maximize quantity and purity of low-abundance fragments.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, April 10, 2017 at 1:00 PM Eastern    Status: Available Reserve

Critical Factors for Successful Multiplex Real-Time PCR

Multiplex real-time PCR is a powerful tool for gene expression analysis, viral load monitoring, genotyping, and many other applications. The ability to amplify and detect several genomic DNA, cDNA, or RNA targets in the same reaction offers many benefits: • Conservation of precious samples – more quantification data per sample • Increased throughput – more targets analyzed per run on a cycler • Reliable results – no well-to-well variability due to co-amplification of internal control • Reduced costs – save time and reagents The QuantiFast Multiplex PCR and RT-PCR kits are optimized for reliable amplification of many different templates despite a high variability in abundance. Thus they enable successful amplification of multiple targets on the first attempt without optimization. This webinar explains the principles of the QIAGEN multiplex technologies and shows data demonstrating the exceptional multiplex real-time PCR performance of the QuantiFast Multiplex kits.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, April 20, 2017 at 9:30 AM Eastern    Status: Available Reserve

The importance of controls and novel solutions for successful real-time qPCR

"The increasing demand for streamlined, monitored, and ultrafast qPCR procedures requires high-performance, real-time quantitative RT and PCR chemistries. Particularly procedures utilizing generic kits for gene expression analysis should include in-process safety measures to avoid variables and control accuracy of procedures and results. This webinar presents innovative solutions for one-step and two-step RT-PCR that significantly enhance performance and reliability in qRT-PCR. The new QuantiNova kit family offers a combination of various integrated safety features to remove variables and prevent artifacts. Internal Control RNA, removal of genomic DNA, room temperature set up capability for RT-PCR, and a built-in visual pipetting control verify accurate procedures, ensuring reliable gene expression profiling. This webinar explains the principles of the technologies and shows data demonstrating performance in qRT-PCR. Find out how you can verify accurate performance in qRT-PCR and improve your results."

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, March 28, 2017 at 9:30 AM Eastern    Status: Available Reserve
Tuesday, March 28, 2017 at 1:00 PM Eastern    Status: Available Reserve

Noncoding RNAs in cardiovascular disease – potential as biomarkers and more

"Cardiovascular diseases (CVD) are the leading cause of death worldwide, and are therefore the subject of intense, urgent research. Biomarkers could help physicians diagnose heart diseases early, for example, and better therapies could improve survival or healing following events like myocardial infarction. Small noncoding RNAs called microRNAs have recently stepped into the spotlight as circulating biomarkers for a number of diseases, and may also have utility in someday treating CVD more effectively. In this webinar, Ali Bierly will discuss why and how microRNAs are being investigated as biomarkers for CVD, as well as examining some recent findings in the field. Join us to find out how scientists are investigating noncoding RNA involvement in CVD and how you can do the same in your laboratory! "

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, April 18, 2017 at 1:00 PM Eastern    Status: Available Reserve

Studying the microbiome: tools for microbial detection and host-response analysis

The research community has begun correlating the makeup of individual microbiomes with disorders and diseases such as autism, atherosclerosis, obesity and cancer. To accomplish this, researchers must first identify and characterize these microbial communities. This webinar will provide you with a complete overview of the microbiome, metagenomics and host-pathogen interactions. Experimental strategies to facilitate your microbiome research will be discussed. We will also present a variety of Sample to Insight workflows that integrate MO BIO’s nucleic acid isolation technology with QIAGEN’s microbial qPCR and NGS research tools.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, March 29, 2017 at 9:30 AM Eastern    Status: Available Reserve

Exosome Research - Roles of Exosomes in Human Disease and Advanced Isolation Tools

Exosomes and microvesicles function as tiny shuttles to transfer information between donor and recipient cells. These small vesicles have recently attracted a tremendous amount of attention from the scientific community. In this webinar, we discuss the current understanding of their biogenesis, molecular phenotype and functional mechanisms. We will also summarize their crucial roles in the progression of several human diseases including neurodegenerative disease, cancer, cardiovascular disease, and inflammatory diseases. Challenges of enriching and extracting exosomes and other microvesicles will be discussed and new solutions will be presented.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, April 3, 2017 at 1:00 PM Eastern    Status: Available Reserve

Characterization of RNA from Extracellular Vesicles - Challenges and Solutions

Exosomes and other extracellular vesicles (EVs) such as microvesicles carry functional cargo and play an important role in disease progression. Recently exosomal RNAs, especially microRNAs, have attracted tremendous interest as potential circulating diagnostic biomarkers. This webinar presents an integrated system for identification and characterization of specific RNA molecules from exosomes and other extracellular vesicles (EVs). Advanced assay technologies for characterization of the RNA profiles including mRNA, microRNA and long non-coding RNAs (RNAs) from serum exosomes will be introduced and discussed.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, April 17, 2017 at 1:00 PM Eastern    Status: Available Reserve

Transcriptome analysis of Kupffer cells after uptake of pancreatic cancer exosomes reveal pathways and biological processes involved in metastatic progression

Pancreatic cancer is one of the most lethal malignancies with a poor prognosis. Understanding the mechanisms of tumor progression is therefore essential. Liquid biopsies are non-invasive methods for diagnostics to detect early stage cancer resulting in more successful treatment. One liquid biopsy technique is the detection of RNA from tumor- derived exosomes. These exosomes participate in generating a metastatic niche. Using QIAGEN Bioinformatics solutions with a publicly available dataset we have examined the transcriptome of Kupffer cells after uptake of pancreatic tumor-derived exosomes that induce the formation of a liver metastatic niche and analyzed the pathways and biological processes involved in this process.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, April 24, 2017 at 1:00 PM Eastern    Status: Available Reserve

Digital sequencing technology in oncology: introduction and applications

Next-generation sequencing (NGS) has recently been adopted in the field of oncology to advance personalized treatment of cancer. This webinar discusses the current utilization and challenges of targeted DNA sequencing in oncology. The main challenge with many of today’s targeted DNA sequencing approaches is the generation of errors during amplification steps; these errors limit the ability of a researcher to confidently call low-frequency DNA variants. Here, we will introduce a powerful and novel digital sequencing approach, based on unique molecular indices (UMIs) technology, to detect low-frequency variants with high specificity and sensitivity. UMIs have been used in the development of several sequencing products – including targeted DNA enrichment panels and RNA sequencing – to scan for known and novel fusion genes, as well as sequencing miRNAs.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, April 6, 2017 at 9:30 AM Eastern    Status: Available Reserve

Introduction to miRNA-seq using unique molecular indices and gel-free library construction

"In this webinar, we will introduce the newest NGS solution from QIAGEN, the QIAseq miRNA Library Kit. miRNA sequencing has the potential to uncover new miRNAs, identify processing intermediates and also quantitative differences between samples. However, challenges such as FFPE and serum/plasma samples, the need for high amounts of sample input and tedious workflows using size selection electrophoresis has led to disappointing and often irreproducible results. QIAGEN has developed a revolutionary new miRNA sequencing kit to remove these limitations for miRNA-seq. Our kit ensures highly reproducible and cost-effective miRNA-seq – regardless of the sample. Whether you’re working with serum, cells, tissues or FFPE samples, proprietary technology provided by the QIAseq miRNA Library Kit ensures removal of adapter-based dimers, which allows you to achieve higher levels of usable miRNA mapped reads. In addition, the kit allows a completely gel-free workflow for maximum convenience, starting with only 1 ng of total RNA. The QIAseq miRNA Library Kit includes a free online data analysis portal for mapping reads and interpreting the unique molecular indices (UMI), allowing you to obtain the most unbiased and highest-fidelity results possible. Join us for this webinar to discover what you’ve been missing in your miRNA-seq experiments. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, April 20, 2017 at 1:00 PM Eastern    Status: Available Reserve

Semi-automated low-throughput workflow for microbial analyses of human stool

The gut microbiota composition changes dramatically throughout ageing and disease. A healthy gut microbiota is typically characterized by large bacterial taxonomic diversity and functional capacity, whereas frailty and ageing are associated with loss of diversity and expansion of more pathogenic bacterial species. However, in order to accurately profile changes in microbial communities, the reproducible isolation of high quality DNA is an important step. Automation allows for convenient, reliable and reproducible isolation of high quality DNA, which can be used directly for downstream sequencing applications. In this webinar, we will discuss the development of a semi-automated workflow to profile the gut microbiota of young and old individuals and identify changes in bacterial composition and function that occur with age. This workflow will help to simplify and streamline the DNA extraction process for samples with high inhibitor content and subsequent microbial community analyses.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, April 24, 2017 at 1:00 PM Eastern    Status: Available Reserve

Methylome sequencing: a high-performance post-bisulfite library construction (PBLC) protocol

Epigenetic changes, such as the methylation of cytosine, have been identified as key factors in various diseases. They play a crucial role in the regulation of important cellular processes, such as gene expression and cellular differentiation. Here, we describe a post-bisulfite library construction (PBLC) protocol that can overcome challenges frequently encountered with the classic whole genome bisulfite sequencing (WGBS) method, including low library yields and PCR amplification bias. We explore the establishment of a PBLC protocol that uses the combination of the EpiTect Fast Bisulfite Kit – for bisulfite conversion of non-methylated cytosines – along with the end-polishing and ligation chemistries of the QIAseq Ultralow Input Library Kit.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, March 27, 2017 at 1:00 PM Eastern    Status: Available Reserve
Tuesday, April 25, 2017 at 9:30 AM Eastern    Status: Available Reserve

miRNA-seq from liquid biopsy: robust detection from the lowest sample amounts

miRNAs impact virtually all areas of biology, and in circulation, they are promising biomarker candidates for both normal and disease biology. miRNAs are protected from degradation in virtually all biofluids by exosomes, Ago2, HDL or other protective proteins, but are expressed at low levels. As a result, expression analysis, particularly using next-generation sequencing (NGS), has proven to be difficult. Traditional small RNA library kits lack the sensitivity or specificity to adequately assess miRNA expression. Libraries prepared with using these kits have been fraught with background products, such as adapter dimers and other RNAs, including hY4 Y RNA. These problems collectively manifest as a low mapping percentage to miRNA, a limited dynamic range and lost discovery potential. QIAGEN’s QIAseq miRNA Library Kit is specifically designed to overcome these challenges. The innovative, gel-free workflow enables the preparation of robust libraries from even the most difficult, low RNA content biofluids. QIAseq miRNA maximizes your on-target miRNA reads, dynamic range and, mostly importantly, your discovery potential.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, April 27, 2017 at 1:00 PM Eastern    Status: Available Reserve

Using Workflows to perform integrated analysis of mutational pattern and expression in RNA-seq data

"In this webinar, we demonstrate how to perform integrated analysis of resequencing and expression data arising from a RNA-seq experiment using Workflows in the Genomics Workbench. The session will include a brief presentation as well as a short live demo with a small NGS dataset. The following topics will be covered during this session: • Creating and running end-to-end Workflows in Genomics • Overview of resequencing tools • Overview of RNA-seq expression analysis • Viewing results using Track List "

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, March 30, 2017 at 4:00 AM Eastern    Status: Available Reserve
Thursday, March 30, 2017 at 1:00 PM Eastern    Status: Available Reserve

Introduction to HGMD Professional

"HGMD provides online access to comprehensive information about published inherited disease mutations helping you save significant time on literature searches. In this webinar we will provide an overview of the HGMD content and frequently used features. Learn more about: • Gene and mutation search options • Mutation reports • Classification of HGMD mutations • Advanced search options A 35 minute demonstration will be followed by 10 minutes of Q&A."

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, April 12, 2017 at 4:00 AM Eastern    Status: Available Reserve
Wednesday, April 12, 2017 at 1:00 PM Eastern    Status: Available Reserve

Increasing productivity in Multiplex PCR applications with QIAxcel

"The most commonly used method for analysis of genotyping assays based on end-point PCR is gel electrophoresis, using manually poured slab gels. This method is extremely labor intensive and exposes users to hazardous chemicals such as ethidium bromide. In addition, resolution of such gels is often poor and detailed analysis of the data in terms of determination of fragment size can be tedious — especially when the data are to be compared with previously analyzed PCR products. This webinar focuses on the challenges of typical genotyping applications and describes possible solutions for these, covering aspects from sample purification to detection of end-point PCR products using the QIAxcel system. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, April 27, 2017 at 9:30 AM Eastern    Status: Available Reserve

Taxonomic profiling using shotgun metagenome data

"In this webinar, we will introduce the latest workflows optimized for shotgun metagenome analysis, which we released with the most recent version of Microbial Genomics Pro Suite – our comprehensive, user-friendly and scalable NGS data analysis platform for microbial genomics. The webinar focuses on how users can, with a few simple steps, analyze shotgun metagenome data to obtain and compare taxonomic profiles of microbial communities. You will also learn how to assess and compare microbial diversity between sample categories, and to carry out statistical comparisons of relative abundance between sample groups in the context of experiment-relevant metadata. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, April 17, 2017 at 1:00 PM Eastern    Status: Available Reserve

QIAscout applications in identification of subpopulation-specific variants

"Although single-cell research is currently gaining momentum, the lack of affordable methods to precisely isolate a single cell from a heterogeneous cell population – without manipulating the cellular status – remains a challenge. QIAscout provides an effective and fast method to isolate and recover viable single cells, ensuring minimal disturbance to the cells. Cells are seeded on an array consisting of 12,000 microrafts, which can be selectively dislodged and transferred to reaction tubes for further processing. This novel single-cell isolation method works in conjunction with most inverted microscopes and is considered the ideal method for separating viable and pure single cells for further downstream analysis or cultivation of clonal subpopulations. Due to the heterogeneity of a cell population, optimized tools are needed to detect cell type-specific variants and to reliably identify isolated single cells based on these specific variants. In this study, we used innovative QIAscout technology to isolate 44 single cells from two different cell lines (22 cells each from LoVo and SW48) and detected cell type-specific variants using whole genome amplification and low-pass sequencing, followed by census-based variant identification. In a second experiment, we were able to selectively isolate single cells of one cell type from a heterogeneous cell population using the QIAscout. Pyrosequencing technology was used to detect cell line-specific SNPs that were identified in the initial experiment and was used as a basis to confirm successful and specific isolation of single cells. In summary, we demonstrated that QIAscout technology allows efficient and reliable isolation of single, targeted cells. We also showed that single cells isolated using the QIAscout can be used to detect subpopulation-specific SNPs using whole genome amplification and low-pass sequencing, followed by the census-based variant calling method."

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, April 4, 2017 at 4:00 AM Eastern    Status: Available Reserve
Tuesday, April 4, 2017 at 1:00 PM Eastern    Status: Available Reserve

Note: Viewers will be asked to register before viewing the previously recorded webinars.