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Online Seminars

SABiosciences is pleased to provide free, on-line educational resources covering relevant tools and techniques for gene and protein expression analysis, and cell-based gene function analysis. We offer live webinars, prerecorded and PDF formatted presentations.

See the instructions for the Web Seminars

December 2016
Monday
Tuesday
Wednesday
Thursday
Friday

1

Introduction to NGS

Tools for microbial detection and host-response analysis

2

5

QPCR Introduction

Circulating exosomes and exoRNA detection

6

Basics of sample prep in RNA research

Introduction to microRNA

7

Digital DNA-seq for cancer research

RNA-seq for biomarker discovery

NGS applications in oncology

8

Innovative NGS Library Construction Technology

Microbiome bioinformatics

9

12

Coding & noncoding RNA analysis using qPCR

Controls and novel solutions for real-time qPCR

13

gDNA collection, storage, and purification

Exosome profiling by RNA-seq Explorer solution

14

Analyzing fusion genes with NGS

Tips and tricks for end-point PCR

15

Advanced NGS library prep for challenging samples

CTC detection and characterization

16

19

20

21

22

23

26

27

28

29

30

Current Seminar Titles Available:

Focus Title
1.  QuantiNova PCR kit The importance of controls and novel solutions for successful real-time qPCR
2.  QIAseq Library PrepAdvanced NGS library prep for challenging samples
3.  Microbial Genomics Pro SuiteData analysis on day one – taxonomic and functional microbiome profiling with the Microbial Genomics Pro Suite
4.  ExosomesCharacterization of RNA from Extracellular Vesicles - Challenges and Solutions
5.  QIAseq NGSTargeted DNA sequencing in oncology – detecting sequence variants with digital sequencing
6.  QIAseq NGSAdvanced NGS technology for oncology – detecting and discovering novel fusion genes with digital sequencing
7.  AdnaTestsThe Her2 miracle in CTCs and its correlation to EMT and tumor stemness
8.  QIAseq miRNA Library KitIntroduction to miRNA-seq using unique molecular indices and gel-free library construction

The importance of controls and novel solutions for successful real-time qPCR

"The increasing demand for streamlined, monitored, and ultrafast qPCR procedures requires high-performance, real-time quantitative RT and PCR chemistries. Particularly procedures utilizing generic kits for gene expression analysis should include in-process safety measures to avoid variables and control accuracy of procedures and results. This webinar presents innovative solutions for one-step and two-step RT-PCR that significantly enhance performance and reliability in qRT-PCR. The new QuantiNova kit family offers a combination of various integrated safety features to remove variables and prevent artifacts. Internal Control RNA, removal of genomic DNA, room temperature set up capability for RT-PCR, and a built-in visual pipetting control verify accurate procedures, ensuring reliable gene expression profiling. This webinar explains the principles of the technologies and shows data demonstrating performance in qRT-PCR. Find out how you can verify accurate performance in qRT-PCR and improve your results."

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, January 31, 2017 at 1:00 PM Eastern    Status: Available Reserve

Advanced NGS library prep for challenging samples

"Rapidly developing next-generation sequencing (NGS) technologies provide highly sensitive methods in discovering and characterizing the genetic information of a variety of samples. However, DNA samples are often limited in quantity, as well as compromised in quality. Such samples are not suitable for standard NGS library construction methods, which commonly require hundreds of nanograms of good-quality DNA. Examples of such challenging clinical samples include circulating DNA, laser capture microdissection (LCM) samples, formalin-fixed paraffin-embedded (FFPE) samples, ancient DNA and chromatin immunoprecipitation (ChIP) samples. In this webinar, we describe the measures that should be taken into consideration while sequencing such challenging samples. We will also present methods that can be used to optimize library construction to efficiently convert small amounts of DNA samples into sequencing libraries. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, January 26, 2017 at 9:30 AM Eastern    Status: Available Reserve

Data analysis on day one – taxonomic and functional microbiome profiling with the Microbial Genomics Pro Suite

"In this webinar, we will introduce the user-friendly Microbial Genomics Pro Suite offering workflows optimized for microbiome profiling, microbial typing and outbreak analysis. The workflows and tools for microbial genomics introduced with this software package further extendthe comprehensive set of genomics, transcriptomics and epigenomics analysis solutions that researchers know from CLC Genomics Workbench. The webinar will focus on how users can, with few simple steps, analyze 16S rRNA data to obtain and compare taxonomic profiles of microbial communities. You will also learn how to assemble and annotate metagenomes to generate functional profiles, and how to carry out statistical comparisons of relative abundance between sample groups in the context of experiment-relevant metadata. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, January 24, 2017 at 1:00 PM Eastern    Status: Available Reserve

Characterization of RNA from Extracellular Vesicles - Challenges and Solutions

Exosomes and other extracellular vesicles (EVs) such as microvesicles carry functional cargo and play an important role in disease progression. Recently exosomal RNAs, especially microRNAs, have attracted tremendous interest as potential circulating diagnostic biomarkers. This webinar presents an integrated system for identification and characterization of specific RNA molecules from exosomes and other extracellular vesicles (EVs). Advanced assay technologies for characterization of the RNA profiles including mRNA, microRNA and long non-coding RNAs (RNAs) from serum exosomes will be introduced and discussed.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, January 24, 2017 at 9:30 AM Eastern    Status: Available Reserve

Targeted DNA sequencing in oncology – detecting sequence variants with digital sequencing

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, January 23, 2017 at 1:00 PM Eastern    Status: Available Reserve

Advanced NGS technology for oncology – detecting and discovering novel fusion genes with digital sequencing

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, January 30, 2017 at 1:00 PM Eastern    Status: Available Reserve

The Her2 miracle in CTCs and its correlation to EMT and tumor stemness

"Breast cancer is the most common cancer in women, accounting for 23% of all cancer diagnoses. Early detection of breast cancer metastasis would allow for changes in treatment strategy before the disease progression becomes apparent. In this webinar, we will demonstrate the enrichment and detection value – as well as the prognostic and predictive value – of the AdnaTestBreast in breast cancer. Using the Combination of Combinations Principle (COCP), AdnaTest BreastCancer enables highly specific immunomagnetic cell selection for enriching circulating tumor cells (CTCs) from peripheral blood. It also allows sensitive analysis of breast cancer-associated gene expression in enriched CTCs by reverse transcription and PCR. The high performance of AdnaTest BreastCancer has been demonstrated in dozens of peer-reviewed publications. Several studies have reported that HER2 expression is different in primary tumors compared to CTCs. In addition, AdnaTestBreast HER2 overexpression profile corresponds to HER2 metastatic phenotype; and HER2 detected by the AdnaTestBreast and AdnaTest EMT-2/StemCell seems to initiate EMT and tumor stemness."

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, January 23, 2017 at 9:30 AM Eastern    Status: Available Reserve

Introduction to miRNA-seq using unique molecular indices and gel-free library construction

"In this webinar, we will introduce the newest NGS solution from QIAGEN, the QIAseq miRNA Library Kit. miRNA sequencing has the potential to uncover new miRNAs, identify processing intermediates and also quantitative differences between samples. However, challenges such as FFPE and serum/plasma samples, the need for high amounts of sample input and tedious workflows using size selection electrophoresis has led to disappointing and often irreproducible results. QIAGEN has developed a revolutionary new miRNA sequencing kit to remove these limitations for miRNA-seq. Our kit ensures highly reproducible and cost-effective miRNA-seq – regardless of the sample. Whether you’re working with serum, cells, tissues or FFPE samples, proprietary technology provided by the QIAseq miRNA Library Kit ensures removal of adapter-based dimers, which allows you to achieve higher levels of usable miRNA mapped reads. In addition, the kit allows a completely gel-free workflow for maximum convenience, starting with only 1 ng of total RNA. The QIAseq miRNA Library Kit includes a free online data analysis portal for mapping reads and interpreting the unique molecular indices (UMI), allowing you to obtain the most unbiased and highest-fidelity results possible. Join us for this webinar to discover what you’ve been missing in your miRNA-seq experiments. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, January 25, 2017 at 9:30 AM Eastern    Status: Available Reserve
Wednesday, January 25, 2017 at 1:00 PM Eastern    Status: Available Reserve

Note: Viewers will be asked to register before viewing the previously recorded webinars.