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Online Seminars

SABiosciences is pleased to provide free, on-line educational resources covering relevant tools and techniques for gene and protein expression analysis, and cell-based gene function analysis. We offer live webinars, prerecorded and PDF formatted presentations.

See the instructions for the Web Seminars

February 2017
Monday
Tuesday
Wednesday
Thursday
Friday

1

Isolate exosomes for protein analysis

2

QuantiFERON: TB diagnosis and treatment

3

6

Introduction to miRNA-seq

7

QIAcout for single cell isolation

8

Maximize quantity and purity of exosomal RNA

9

Introduction to miRNA-seq

Cancer research and challenges of FFPE

10

13

14

15

Circulating exosomes and exoRNA detection

NGS sample QC

16

Controls and novel solutions for real-time qPCR

miRNA-seq in liquid biopsy

17

20

QIAcout for single cell isolation

21

Methylome sequencing

Microbial sample prep

22

Exosome profiling by RNA-seq Explorer solution

23

Her2 miracle in CTC and EMT correlation

24

27

Her2 miracle in CTC and EMT correlation

28

Current Seminar Titles Available:

Focus Title
1.  DNA sample isolationFundamental Concepts and Special Considerations in gDNA Isolation for Better Insight
2.  QuantiNova PCR kit The importance of controls and novel solutions for successful real-time qPCR
3.  Multiplex PCR Multiplex End-Point PCR for Genotyping: Critical factors and applications
4.  Sample Quality ControlNucleic acids quantification from FFPE samples; are you doing it right?
5.  Microbial DNA PCR ArraysStudying the microbiome: tools for microbial detection and host-response analysis
6.  QIAseq NGSDigital sequencing technology in oncology: introduction and applications
7.  QIAseq NGSTargeted DNA sequencing in oncology – detecting sequence variants with digital sequencing
8.  QIAseq NGSAdvanced NGS technology for oncology – detecting and discovering novel fusion genes with digital sequencing
9.  AdnaTestsThe Her2 miracle in CTCs and its correlation to EMT and tumor stemness
10.  QIAseq miRNA Library KitmiRNA-seq from liquid biopsy: robust detection from the lowest sample amounts
11.  Ingenuity Pathway Analysis (IPA)Introduction to IPA and the powerful knowledge base behind it
12.  Ingenuity Pathway Analysis (IPA)Formatting and uploading your data into IPA
13.  Ingenuity Pathway Analysis (IPA)Interpreting the results of your Core Analysis in IPA
14.  CLC Genomics WorkbenchIntroduction to the CLC Genomics Workbench: A preview
15.  CLC Genomics WorkbenchFishing for high confidence variants from NGS data using the CLC Genomics Workbench
16.  CLC Genomics WorkbenchStatistical analysis, visualization and functional enrichment of RNA-seq data in the CLC Genomics Workbench
17.  CLC Genomics WorkbenchUsing Workflows to perform integrated analysis of mutational pattern and expression in RNA-seq data
18.  QIAseq Targeted RNA PanelsTargeted RNA Sequencing for Gene Expression
19.  GeneReader NGS System Case study: Implementing NGS in your lab in 30 days

Fundamental Concepts and Special Considerations in gDNA Isolation for Better Insight

"How are your gDNA yields? Some sample types present special challenges in DNA purification and analysis. This webinar provides tips for a whole range of sample types that require special consideration. We will cover the following topics in detail: • The basic methods and challenges of gDNA purification • Working with DNA: good laboratory practice • Special considerations for challenging sample types • Performing DNA quality checks"

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, March 6, 2017 at 1:00 PM Eastern    Status: Available Reserve

The importance of controls and novel solutions for successful real-time qPCR

"The increasing demand for streamlined, monitored, and ultrafast qPCR procedures requires high-performance, real-time quantitative RT and PCR chemistries. Particularly procedures utilizing generic kits for gene expression analysis should include in-process safety measures to avoid variables and control accuracy of procedures and results. This webinar presents innovative solutions for one-step and two-step RT-PCR that significantly enhance performance and reliability in qRT-PCR. The new QuantiNova kit family offers a combination of various integrated safety features to remove variables and prevent artifacts. Internal Control RNA, removal of genomic DNA, room temperature set up capability for RT-PCR, and a built-in visual pipetting control verify accurate procedures, ensuring reliable gene expression profiling. This webinar explains the principles of the technologies and shows data demonstrating performance in qRT-PCR. Find out how you can verify accurate performance in qRT-PCR and improve your results."

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, March 28, 2017 at 9:30 AM Eastern    Status: Available Reserve
Tuesday, March 28, 2017 at 1:00 PM Eastern    Status: Available Reserve

Multiplex End-Point PCR for Genotyping: Critical factors and applications

"Multiplex endpoint PCR is a powerful tool for genotyping and many other applications. The ability to amplify and detect several DNA targets in the same reaction offers many benefits. It enables generation of more data out of less sample material, which is especially beneficial in routines with limited and precious sample material. It also offers the opportunity to run internal controls in every reaction, thus increasing the overall reliability of data acquisition. Most importantly, switching PCR-based routine lab testing to multiplex PCR allows you to save time, materials and cost. This webinar discusses the critical factors in planning and performing multiplex PCR. We will describe an optimized multiplex PCR chemistry for reliable amplification of multiple templates with high variability in copy numbers. Come and learn how QIAGEN's multiplex PCR chemistry allows you to easily set up multiplex PCR. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, March 20, 2017 at 1:00 PM Eastern    Status: Available Reserve

Nucleic acids quantification from FFPE samples; are you doing it right?

"Formalin-fixation and paraffin-embedding is a standard method for long-term preservation of tissue biopsies and these stored samples are a valuable tool for studying diseases such as cancer, especially when they are histologically and pathologically well characterized, and follow-up clinical data is available. The quality of nucleic acids extracted from FFPE samples is influenced by a number of factors, including how the samples were handled before, during and after fixation & embedding. Moreover, there are several difficulties when purifying nucleic acids from FFPE samples as the chemicals & temperature used during the process can degrade the DNA. In this webinar we will discuss the variability in quantity and purity of DNA purified from FFPE material. We will show data from different quantification and quality control methods and demonstrate the impact of inaccurate quantification on downstream results and how to overcome these challenges. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, March 13, 2017 at 1:00 PM Eastern    Status: Available Reserve

Studying the microbiome: tools for microbial detection and host-response analysis

The research community has begun correlating the makeup of individual microbiomes with disorders and diseases such as autism, atherosclerosis, obesity and cancer. To accomplish this, researchers must first identify and characterize these microbial communities. This webinar will provide you with a complete overview of the microbiome, metagenomics and host-pathogen interactions. Experimental strategies to facilitate your microbiome research will be discussed. We will also present a variety of Sample to Insight workflows that integrate MO BIO’s nucleic acid isolation technology with QIAGEN’s microbial qPCR and NGS research tools.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, March 29, 2017 at 9:30 AM Eastern    Status: Available Reserve

Digital sequencing technology in oncology: introduction and applications

Next-generation sequencing (NGS) has recently been adopted in the field of oncology to advance personalized treatment of cancer. This webinar discusses the current utilization and challenges of targeted DNA sequencing in oncology. The main challenge with many of today’s targeted DNA sequencing approaches is the generation of errors during amplification steps; these errors limit the ability of a researcher to confidently call low-frequency DNA variants. Here, we will introduce a powerful and novel digital sequencing approach, based on unique molecular indices (UMIs) technology, to detect low-frequency variants with high specificity and sensitivity. UMIs have been used in the development of several sequencing products – including targeted DNA enrichment panels and RNA sequencing – to scan for known and novel fusion genes, as well as sequencing miRNAs.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, March 7, 2017 at 1:00 PM Eastern    Status: Available Reserve

Targeted DNA sequencing in oncology – detecting sequence variants with digital sequencing

Targeted DNA sequencing has enabled efficient and accurate detection of novel and rare somatic mutations in oncology. This powerful approach achieves high coverage of the region of interest while keeping the sequencing cost and the complexity of data interpretation manageable. However, existing PCR-based target enrichment approaches introduce errors due to PCR amplification bias and artifacts. Such errors significantly affect quantification accuracy and limit the ability to confidently detect low-frequency DNA variants. This webinar introduces a new digital sequencing approach that is based on the use of unique molecular indices (UMIs): the QIAseq Targeted DNA Panels. With UMIs, each unique DNA molecule is barcoded before any amplification takes place to correct for PCR errors. Detailed workflow and several applications in oncology research will be presented. Join us and learn about this exciting new digital DNAseq technology.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, March 14, 2017 at 1:00 PM Eastern    Status: Available Reserve

Advanced NGS technology for oncology – detecting and discovering novel fusion genes with digital sequencing

Given their association with tumorigenesis, fusion genes are attractive targets for developing new drugs and identifying relevant biomarkers. And while NGS has recently been used to discover and identify fusion genes, the current method is complicated, expensive and requires relatively large amount of samples. In this webinar, we will introduce the QIAseq Targeted RNAscan Panels – a novel, complete Sample to Insight solution that applies the unique molecular indices (UMIs) strategy to detect known and new fusion genes. The system enables the detection of a large number of fusion genes as well as identification of new fusion gene partners, up to 384 samples per sequencing run. It is suitable for RNA samples from FFPE, blood and plasma with input as low as 15 ng of un-enriched RNA. Experiment workflow and application data will be presented. Join us and learn how you can use this powerful tool to detect and discover known and novel fusion genes.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, March 21, 2017 at 1:00 PM Eastern    Status: Available Reserve

The Her2 miracle in CTCs and its correlation to EMT and tumor stemness

"Breast cancer is the most common cancer in women, accounting for 23% of all cancer diagnoses. Early detection of breast cancer metastasis would allow for changes in treatment strategy before the disease progression becomes apparent. In this webinar, we will demonstrate the enrichment and detection value – as well as the prognostic and predictive value – of the AdnaTestBreast in breast cancer. Using the Combination of Combinations Principle (COCP), AdnaTest BreastCancer enables highly specific immunomagnetic cell selection for enriching circulating tumor cells (CTCs) from peripheral blood. It also allows sensitive analysis of breast cancer-associated gene expression in enriched CTCs by reverse transcription and PCR. The high performance of AdnaTest BreastCancer has been demonstrated in dozens of peer-reviewed publications. Several studies have reported that HER2 expression is different in primary tumors compared to CTCs. In addition, AdnaTestBreast HER2 overexpression profile corresponds to HER2 metastatic phenotype; and HER2 detected by the AdnaTestBreast and AdnaTest EMT-2/StemCell seems to initiate EMT and tumor stemness."

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, February 27, 2017 at 9:30 AM Eastern    Status: Available Reserve

miRNA-seq from liquid biopsy: robust detection from the lowest sample amounts

miRNAs impact virtually all areas of biology, and in circulation, they are promising biomarker candidates for both normal and disease biology. miRNAs are protected from degradation in virtually all biofluids by exosomes, Ago2, HDL or other protective proteins, but are expressed at low levels. As a result, expression analysis, particularly using next-generation sequencing (NGS), has proven to be difficult. Traditional small RNA library kits lack the sensitivity or specificity to adequately assess miRNA expression. Libraries prepared with using these kits have been fraught with background products, such as adapter dimers and other RNAs, including hY4 Y RNA. These problems collectively manifest as a low mapping percentage to miRNA, a limited dynamic range and lost discovery potential. QIAGEN’s QIAseq miRNA Library Kit is specifically designed to overcome these challenges. The innovative, gel-free workflow enables the preparation of robust libraries from even the most difficult, low RNA content biofluids. QIAseq miRNA maximizes your on-target miRNA reads, dynamic range and, mostly importantly, your discovery potential.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, March 20, 2017 at 9:30 AM Eastern    Status: Available Reserve

Introduction to IPA and the powerful knowledge base behind it

Explore how IPA’s knowledge and discovery tools allow you to relate the most recent literature findings to your research and help you in hypothesis generation. Learn how to use IPA to gain detailed information about genes and isoforms and create interactive and customized pathways using tools such as the BioProfiler. You can leverage all of this information instantly without needing to upload any of your data and create interactive and customized pathways using tools such as the BioProfiler.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, March 1, 2017 at 4:00 AM Eastern    Status: Available Reserve
Wednesday, March 1, 2017 at 1:00 PM Eastern    Status: Available Reserve

Formatting and uploading your data into IPA

"Learn how to format and upload your own data into IPA. IPA can upload your experimental data to enable you to perform pathways visualization, literature searches on the molecules in the dataset and allow you to conduct the many different types of analyses offered in IPA. In this webinar, we will use RNA-sequencing data as an example dataset. Learn how to: • Format the incoming data to be analyzed by IPA • Upload the data to be analyzed • Explore your uploaded data and start a Core Analysis "

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, March 8, 2017 at 4:00 AM Eastern    Status: Available Reserve
Wednesday, March 8, 2017 at 1:00 PM Eastern    Status: Available Reserve

Interpreting the results of your Core Analysis in IPA

"Learn how to view and interpret your Core Analysis results in IPA. You can find out how to understand your Core Analysis and the multiple ways of relating the molecules in your dataset to the body of information in the Ingenuity Knowledge Base. Discover more about: • Biological functions and diseases that are over-represented in your data • Signaling and metabolic canonical pathways enriched in your data • Predicted upstream regulators that might explain the changes observed in your data • How IPA’s integration of analysis results creates causal hypotheses about how upstream regulators influence downstream phenotypes and biological functions • Molecular networks (algorithmically generated pathways describing potential molecular interactions in your experimental system) • Molecules and biological processes that are predicted to be activated or inhibited in your experimental system institutions. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, March 15, 2017 at 4:00 AM Eastern    Status: Available Reserve
Wednesday, March 15, 2017 at 1:00 PM Eastern    Status: Available Reserve

Introduction to the CLC Genomics Workbench: A preview

"This introductory webinar will provide new users with a sneak peek on the basic features of the Genomics Workbench. It will also cover tips and tricks as well as new highlights in the Workbench that will be useful for our more seasoned users. The webinar includes a brief presentation as well as a short live demo with a small NGS dataset. We will be focusing on the following topics: • Introduction to QIAGEN’s bioinformatics portfolio • Overview of the Workbench user interface • Plugins • Customer self-help resources • Running individual tools • Import, QC and pre-processing of NGS data • Import of reference genome data • Export of data • Introduction to Workflows • Batch analysis of data "

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, March 9, 2017 at 4:00 AM Eastern    Status: Available Reserve
Thursday, March 9, 2017 at 9:30 AM Eastern    Status: Available Reserve

Fishing for high confidence variants from NGS data using the CLC Genomics Workbench

"Speaker: Dr. Prakriti Mudvari In this webinar, we will demonstrate how to identify high confidence genetic variants starting from raw sequencing reads in the Genomics Workbench. The session will include a brief presentation as well as a short live demo with a small NGS dataset. We will be covering the topics below during the webinar: • Mapping of raw reads to reference • QC tools • Pipeline for improving confidence of variant detection • Overview of variant detectors • Basic and noise filters • Comparison and annotation of variants • Examining functional consequences of identified variants • Visualization of data "

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, March 16, 2017 at 4:00 AM Eastern    Status: Available Reserve
Thursday, March 16, 2017 at 1:00 PM Eastern    Status: Available Reserve

Statistical analysis, visualization and functional enrichment of RNA-seq data in the CLC Genomics Workbench

"This webinar presents how to analyze RNA-seq data starting from raw sequencing reads in the Genomics Workbench. The session will include a brief presentation as well as a short live demo with a small NGS dataset. We will be covering the topics below during the webinar: • Mapping of reads to the reference and abundance estimation • Principal component analysis (PCA) of RNA-seq data • Statistical analysis of differential expression • Visualization of results using volcano plots and Venn diagrams • Creating RNA-seq expression table and adding GO annotations • Gene set enrichment analysis using hypergeometric test "

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, March 23, 2017 at 4:00 AM Eastern    Status: Available Reserve
Thursday, March 23, 2017 at 1:00 PM Eastern    Status: Available Reserve

Using Workflows to perform integrated analysis of mutational pattern and expression in RNA-seq data

"In this webinar, we demonstrate how to perform integrated analysis of resequencing and expression data arising from a RNA-seq experiment using Workflows in the Genomics Workbench. The session will include a brief presentation as well as a short live demo with a small NGS dataset. The following topics will be covered during this session: • Creating and running end-to-end Workflows in Genomics • Overview of resequencing tools • Overview of RNA-seq expression analysis • Viewing results using Track List "

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, March 30, 2017 at 4:00 AM Eastern    Status: Available Reserve
Thursday, March 30, 2017 at 1:00 PM Eastern    Status: Available Reserve

Targeted RNA Sequencing for Gene Expression

"Quantitative gene expression on NGS instruments has been crippled by amplicon based target capture which results in large amounts of biased library due to PCR duplicates and PCR based amplicon problems. QIAseq Targeted RNA Panels solve this limitation by using unique molecular indexes (UMIs) and single primer extension (SPE). QIAGEN’s simple 1-day library construction protocol can be utilized by any lab with a pipette, thermocycler, magnet and access to an NGS instrument. Our integrated data analysis options allow researchers to quickly turn raw sequencing files into biologically relevant information. T In this seminar, we will review the principles of digital sequencing with a focus on gene expression applications for illumina and Thermo Fisher sequencing instruments. Qualifications questions during login: 1.) Are you doing gene expression studies with FFPE or less than 10 ng Total RNA? 2.) Do you have access to an NGS instrument in your lab or core facility?"

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, March 7, 2017 at 4:00 PM Eastern    Status: Available Reserve
Thursday, March 9, 2017 at 9:30 AM Eastern    Status: Available Reserve

Case study: Implementing NGS in your lab in 30 days

"Identification of the many complex genetic aberrations associated with cancers, and deciphering their role in disease progression and response to treatment is one of the major challenges faced by oncology researchers. Next-Generation Sequencing has the potential to provide unprecedented insight into the complex molecular pathology of diseases such as cancer. However, broad adoption of NGS technology by small and medium sized laboratories can be daunting due to the inherent complexity of the technology, workflow fragmentation and cost obscurity, all of which lead to protracted investment of time and resources. The QIAGEN GeneReader NGS System was developed specifically with small to medium throughput laboratories in mind. The system is the world’s first truly Sample to Insight NGS workflow integrating all upstream sample processing steps, as well as downstream bioinformatics analysis and interpretation. It has the added benefit of the standardization offered by a single vendor workflow solution, and scalability options to fit laboratories individual throughput needs. This is an all-in-one solution that enables any research lab to chart the complex cancer genetic map while also providing up-to-date information, curated in the QIAGEN world leading Knowledge base that can be used to guide result interpretation. In today’s webinar, Salim Essakali will highlight the benefits of a complete NGS system that can lead you from nucleic acid extraction, through sequencing template preparation and sequencing to analysis and interpretation of the data generated. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, March 14, 2017 at 1:00 PM Eastern    Status: Available Reserve

Note: Viewers will be asked to register before viewing the previously recorded webinars.