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Online Seminars

SABiosciences is pleased to provide free, on-line educational resources covering relevant tools and techniques for gene and protein expression analysis, and cell-based gene function analysis. We offer live webinars, prerecorded and PDF formatted presentations.

See the instructions for the Web Seminars

March 2017
Monday
Tuesday
Wednesday
Thursday
Friday

1

Introduction to IPA

Introduction to IPA

2

3

6

gDNA collection, storage, and purification

7

NGS in oncology: digital NGS technology intro

Targeted RNA Sequencing for Gene Expression

8

Formatting and uploading your data into IPA

Formatting and uploading your data into IPA

9

Targeted RNA Sequencing for Gene Expression

CLC Genomics Workbench: Introduction

CLC Genomics Workbench: Introduction

10

13

FFPE sample QC

14

NGS in oncology: digital DNA enrichment

NGS live in your lab in 30 days’

15

Interpreting the results of your Core Analysis in IPA

Interpreting the results of your Core Analysis in IPA

16

CLC Genomics Workbench: Variant analysis

CLC Genomics Workbench: Variant analysis

17

20

miRNA-seq in liquid biopsy

Multiplex PCR as a tool for genotyping

21

NGS in oncology: detecting fusion genes

22

23

CLC Genomics Workbench: RNA-seq

CLC Genomics Workbench: RNA-seq

24

27

Methylome sequencing

28

Controls and novel solutions for real-time qPCR

Controls and novel solutions for real-time qPCR

29

Tools for microbial detection and host-response analysis

30

CLC Genomics Workbench: RNA-seq and mutation analysis

CLC Genomics Workbench: RNA-seq and mutation analysis

31

Current Seminar Titles Available:

Focus Title
1.  Ingenuity Pathway Analysis (IPA)Introduction to IPA and the powerful knowledge base behind it
2.  Ingenuity Pathway Analysis (IPA)Formatting and uploading your data into IPA
3.  Ingenuity Pathway Analysis (IPA)Interpreting the results of your Core Analysis in IPA
4.  QIAscoutQIAscout applications in identification of subpopulation-specific variants
5.  QIAseq NGS Library KitQIAseq Technologies for Low Input Whole Genome Sequencing
6.  QuantiFERON-TBAn Update: Breakthrough Research on IGRA Use in Pediatric TB testing
7.  QIAseq NGSDigital sequencing: Introduction to Unique Molecular Index (UMI) technology for targeted sequencing
8.  QIAseq NGSTargeted DNA-seq for mutation detection
9.  QIAseq NGSGene expression analysis using targeted RNA-seq and desktop NGS instruments
10.  QIAseq NGSLinking miRNA and gene expression using digital NGS
11.  QIAseq NGSNext-generation sequencing in single-cell applications
12.  AutomationReproducibility, quality control and importance of automation
13.  Liquid BiopsiesIsolation and molecular characterization of single CTCs after AdnaTest immunomagnetic pre-enrichment
14.  QIAseq NGSDigital sequencing technology for hematologic malignancies and solid tumors

Introduction to IPA and the powerful knowledge base behind it

Explore how IPA’s knowledge and discovery tools allow you to relate the most recent literature findings to your research and help you in hypothesis generation. Learn how to use IPA to gain detailed information about genes and isoforms and create interactive and customized pathways using tools such as the BioProfiler. You can leverage all of this information instantly without needing to upload any of your data and create interactive and customized pathways using tools such as the BioProfiler.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, May 3, 2017 at 4:00 AM Eastern    Status: Available Reserve
Wednesday, May 3, 2017 at 1:00 PM Eastern    Status: Available Reserve

Formatting and uploading your data into IPA

"Learn how to format and upload your own data into IPA. IPA can upload your experimental data to enable you to perform pathways visualization, literature searches on the molecules in the dataset and allow you to conduct the many different types of analyses offered in IPA. In this webinar, we will use RNA-sequencing data as an example dataset. Learn how to: • Format the incoming data to be analyzed by IPA • Upload the data to be analyzed • Explore your uploaded data and start a Core Analysis "

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, May 10, 2017 at 4:00 AM Eastern    Status: Available Reserve
Wednesday, May 10, 2017 at 1:00 PM Eastern    Status: Available Reserve

Interpreting the results of your Core Analysis in IPA

"Learn how to view and interpret your Core Analysis results in IPA. You can find out how to understand your Core Analysis and the multiple ways of relating the molecules in your dataset to the body of information in the Ingenuity Knowledge Base. Discover more about: • Biological functions and diseases that are over-represented in your data • Signaling and metabolic canonical pathways enriched in your data • Predicted upstream regulators that might explain the changes observed in your data • How IPA’s integration of analysis results creates causal hypotheses about how upstream regulators influence downstream phenotypes and biological functions • Molecular networks (algorithmically generated pathways describing potential molecular interactions in your experimental system) • Molecules and biological processes that are predicted to be activated or inhibited in your experimental system institutions. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, May 17, 2017 at 4:00 AM Eastern    Status: Available Reserve
Wednesday, May 17, 2017 at 1:00 PM Eastern    Status: Available Reserve

QIAscout applications in identification of subpopulation-specific variants

"Although single-cell research is currently gaining momentum, the lack of affordable methods to precisely isolate a single cell from a heterogeneous cell population – without manipulating the cellular status – remains a challenge. QIAscout provides an effective and fast method to isolate and recover viable single cells, ensuring minimal disturbance to the cells. Cells are seeded on an array consisting of 12,000 microrafts, which can be selectively dislodged and transferred to reaction tubes for further processing. This novel single-cell isolation method works in conjunction with most inverted microscopes and is considered the ideal method for separating viable and pure single cells for further downstream analysis or cultivation of clonal subpopulations. Due to the heterogeneity of a cell population, optimized tools are needed to detect cell type-specific variants and to reliably identify isolated single cells based on these specific variants. In this study, we used innovative QIAscout technology to isolate 44 single cells from two different cell lines (22 cells each from LoVo and SW48) and detected cell type-specific variants using whole genome amplification and low-pass sequencing, followed by census-based variant identification. In a second experiment, we were able to selectively isolate single cells of one cell type from a heterogeneous cell population using the QIAscout. Pyrosequencing technology was used to detect cell line-specific SNPs that were identified in the initial experiment and was used as a basis to confirm successful and specific isolation of single cells. In summary, we demonstrated that QIAscout technology allows efficient and reliable isolation of single, targeted cells. We also showed that single cells isolated using the QIAscout can be used to detect subpopulation-specific SNPs using whole genome amplification and low-pass sequencing, followed by the census-based variant calling method."

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, May 9, 2017 at 9:30 AM Eastern    Status: Available Reserve

QIAseq Technologies for Low Input Whole Genome Sequencing

"Rapidly developing next-generation sequencing (NGS) technologies provide highly sensitive methods in discovering and characterizing the genetic information of a variety of samples. However, DNA samples are often limited in quantity, as well as compromised in quality. Such samples are not suitable for standard NGS library construction methods, which commonly require hundreds of nanograms of good-quality DNA. Examples of such challenging clinical samples include circulating DNA, laser capture microdissection (LCM) samples, formalin-fixed paraffin-embedded (FFPE) samples, ancient DNA and chromatin immunoprecipitation (ChIP) samples. In this webinar, we describe the measures that should be taken into consideration while sequencing such challenging samples. We will also present methods that can be used to optimize library construction to efficiently convert small amounts of DNA samples into sequencing libraries, especially for whole genome sequencing applications. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, May 8, 2017 at 9:30 AM Eastern    Status: Available Reserve
Monday, May 15, 2017 at 9:30 AM Eastern    Status: Available Reserve

An Update: Breakthrough Research on IGRA Use in Pediatric TB testing

"A number of recent research studies detail the potential use of IGRAs to test for TB infection in pediatric populations. These include a milestone study that indicated, for the first time, a test’s ability to predict a group of children who were more likely to progress to active tuberculosis using QuantiFERON-TB Gold. Join Dr. Islam as he reviews recent studies and current pediatric guidance on IGRAs and discusses the topic, “TST preferred in children under 5: Is there enough data to say otherwise?” Shamim Islam, MD is Clinical Assistant Professor and Attending Physician at the University at Buffalo SUNY. Dr. Islam is a former Pediatric Infectious Disease Physician as well as a former Clinic Physician in San Francisco TB Control Program. The performance of the USA format of the QuantiFERON-TB Gold test has not been extensively evaluated with specimens from individuals younger than age 17 years"

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, May 22, 2017 at 9:30 AM Eastern    Status: Available Reserve

Digital sequencing: Introduction to Unique Molecular Index (UMI) technology for targeted sequencing

Errors related to the use of PCR in library construction have plagued targeted DNA and RNA sequencing experiments, calling into question the accuracy of results. Digital sequencing overcomes these challenges by introducing Unique Molecular Indices, or UMIs. These molecular barcodes are incorporated into the DNA or RNA sequences before any amplification takes place, eliminating errors such as PCR duplication and amplification bias. This webinar features an in-depth discussion of how UMI technology works and an introduction to how it is used in QIAGEN’s QIAseq sequencing solutions. Find out how to improve your NGS data with the accuracy of UMIs!

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, May 22, 2017 at 11:00 AM Eastern    Status: Available Reserve

Targeted DNA-seq for mutation detection

The utilization of targeted DNA sequencing with enrichment panels is on the rise for detecting genetic variants in cancers, inherited diseases and more. In this webinar, we introduce the QIAseq Targeted DNA Panels, which use Unique Molecular Indices (UMIs) and Single Primer Extension (SPE) technology to improve downstream analysis of difficult-to-sequence genes on Illumina and Thermo Fisher NGS instruments. QIAGEN uses its proprietary knowledge base to design panels for different cancer types, carrier testing, cardiomyopathy and other areas of interest. In addition, QIAGEN offers full coverage of the human genome, allowing researchers to build custom panels for their specific applications. Join us to find out how you can quickly and reliably detect genetic variants in your research from FFPE and fresh samples.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, May 23, 2017 at 11:00 AM Eastern    Status: Available Reserve

Gene expression analysis using targeted RNA-seq and desktop NGS instruments

Not every experiment requires whole transcriptome sequencing, but until recently, there hasn’t been a convenient way to profile transcriptomes in biological research areas such as cancer or immunity. In this webinar, we discuss the strategy and technology of targeted RNA sequencing, including the use of novel targeted RNA-seq panels covering the transcriptomes of 8 different pathways for human and mouse, as well as 170+ smaller panels. Using Single Primer Extension (SPE) and Unique Molecular Index (UMI) technology, the QIAseq panels overcome traditional RNA-seq limitations and ensure increased precision and accuracy. Join us and learn how you can easily upgrade your RNA-seq data and move beyond the limits of qPCR, microarray and traditional RNA-seq.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, May 24, 2017 at 11:00 AM Eastern    Status: Available Reserve

Linking miRNA and gene expression using digital NGS

Total RNA discovery includes exploring both gene expression and regulation. This means analyzing both mRNA and noncoding regulatory RNA species such as microRNA. Until recently, successful miRNA-seq was difficult due to challenges such as contamination with adapter dimers and other RNA species, PCR bias and high sample input requirements. In this webinar, we discuss how new technological advances have helped overcome the challenges of miRNA-seq and RNA-seq, and why NGS is replacing qPCR as the technology of choice for total RNA discovery studies. Join us to find out about the recent advances in RNA sequencing and to learn about a case study in which NGS links microRNA with gene expression analysis.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, May 25, 2017 at 11:00 AM Eastern    Status: Available Reserve

Next-generation sequencing in single-cell applications

Single-cell sequencing is revolutionizing how we understand biology by revealing the individual contributions of cells rather than simply analyzing in bulk. QIAGEN has developed complete cell-to-library solutions for DNA and RNA sequencing libraries from single cells, as well as the novel QIAscout system for reliable single-cell isolation. In this webinar, we discuss several applications for single-cell NGS, along with application data. Find out how to apply NGS to your single-cell research!

Duration: 45 minutes followed by Q&A session.

Schedule:

Friday, May 26, 2017 at 11:00 AM Eastern    Status: Available Reserve

Reproducibility, quality control and importance of automation

"From starting material to final results, every analysis workflow is a journey to unlock the biological information within your samples without altering it, and quality results are only achieved from quality samples. Within each step lie challenges directly related to the sample type and analysis technologies, and at each step, there is potential for many things to go wrong, jeopardizing your experiments, results and reputation. Therefore, standardizing samples and performing relevant quality control after critical steps is of utmost importance to ensure quality and reproducibility of results as well as reliable interpretation. In this webinar, we will introduce you to the main sample quality parameters, their respective impact on downstream applications, how to monitor them and what are the advantages automating quality control along complex workflows."

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, May 18, 2017 at 9:30 AM Eastern    Status: Available Reserve

Isolation and molecular characterization of single CTCs after AdnaTest immunomagnetic pre-enrichment

Circulating tumor cells (CTCs) are present in different types, including cytokeratin-positive CTCs, stem cell CTCs, apoptotic CTCs and small CTCs. The ability to isolate single CTCs and characterize their patient-specific expression profile is promising for diagnosis and clinical treatment. In this webinar, Dr. Norbert Hochstein and Dr. Siegfried Hauch introduce the latest development – QIAscout single cell technology and AdnaTest technology, for isolation and molecular characterization of single CTCs. Detailed experimental design and data will be presented to demonstrate that single CTCs that have been pre-enriched with AdnaTest ProstateCancerSelect technology are suitable for isolation using the QIAscout system. Furthermore, the single CTCs isolated with the QIAscout system are compatible with downstream mRNA profiling using AdnaTest ProstateCancerDetect. The combined technologies offer an efficient workflow and a powerful tool for analysis of single CTCs to help elucidate the heterogeneous character of single cells and the analysis of rare cells. Come and learn how you can apply these advanced technologies for your liquid biopsy research.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, May 24, 2017 at 9:30 AM Eastern    Status: Available Reserve

Digital sequencing technology for hematologic malignancies and solid tumors

Cancer is the result of the accumulation of multiple abnormalities over time that can disrupt normal cellular function & allow cells to proliferate unregulated, survive, invade, metastasize. The cause of this unregulated growth can be due to a number of genomic changes, including fusion genes – hybrid genes formed from 2 originally separate genes – and DNA alterations including somatic mutations, SNPs and copy number variations. In this webinar, we will review the history and mechanisms of hematologic malignancies and solid tumors, as well as discussing current detection technologies and new digital NGS technologies for cancer research. We will introduce the new digital sequencing approach, which is based on the unique molecular indices (UMIs) method to provide high specificity, sensitivity and limit PCR duplicates and errors. Detailed workflow and applications in hematologic malignancies and solid tumors will be highlighted. Join us and learn about this exciting new digital sequencing technology.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, May 11, 2017 at 1:00 PM Eastern    Status: Available Reserve

Note: Viewers will be asked to register before viewing the previously recorded webinars.