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Online Seminars

SABiosciences is pleased to provide free, on-line educational resources covering relevant tools and techniques for gene and protein expression analysis, and cell-based gene function analysis. We offer live webinars, prerecorded and PDF formatted presentations.

See the instructions for the Web Seminars

April 2017
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Current Seminar Titles Available:

Focus Title
1.  QuantiNova PCR kit The importance of controls and novel solutions for successful real-time qPCR
2.  Microbial DNA PCR ArraysStudying the microbiome: tools for microbial detection and host-response analysis
3.  QIAseq Library PrepMethylome sequencing: a high-performance post-bisulfite library construction (PBLC) protocol
4.  CLC Genomics WorkbenchUsing Workflows to perform integrated analysis of mutational pattern and expression in RNA-seq data

The importance of controls and novel solutions for successful real-time qPCR

"The increasing demand for streamlined, monitored, and ultrafast qPCR procedures requires high-performance, real-time quantitative RT and PCR chemistries. Particularly procedures utilizing generic kits for gene expression analysis should include in-process safety measures to avoid variables and control accuracy of procedures and results. This webinar presents innovative solutions for one-step and two-step RT-PCR that significantly enhance performance and reliability in qRT-PCR. The new QuantiNova kit family offers a combination of various integrated safety features to remove variables and prevent artifacts. Internal Control RNA, removal of genomic DNA, room temperature set up capability for RT-PCR, and a built-in visual pipetting control verify accurate procedures, ensuring reliable gene expression profiling. This webinar explains the principles of the technologies and shows data demonstrating performance in qRT-PCR. Find out how you can verify accurate performance in qRT-PCR and improve your results."

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, March 28, 2017 at 9:30 AM Eastern    Status: Available Reserve
Tuesday, March 28, 2017 at 1:00 PM Eastern    Status: Available Reserve

Studying the microbiome: tools for microbial detection and host-response analysis

The research community has begun correlating the makeup of individual microbiomes with disorders and diseases such as autism, atherosclerosis, obesity and cancer. To accomplish this, researchers must first identify and characterize these microbial communities. This webinar will provide you with a complete overview of the microbiome, metagenomics and host-pathogen interactions. Experimental strategies to facilitate your microbiome research will be discussed. We will also present a variety of Sample to Insight workflows that integrate MO BIO’s nucleic acid isolation technology with QIAGEN’s microbial qPCR and NGS research tools.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, March 29, 2017 at 9:30 AM Eastern    Status: Available Reserve

Methylome sequencing: a high-performance post-bisulfite library construction (PBLC) protocol

Epigenetic changes, such as the methylation of cytosine, have been identified as key factors in various diseases. They play a crucial role in the regulation of important cellular processes, such as gene expression and cellular differentiation. Here, we describe a post-bisulfite library construction (PBLC) protocol that can overcome challenges frequently encountered with the classic whole genome bisulfite sequencing (WGBS) method, including low library yields and PCR amplification bias. We explore the establishment of a PBLC protocol that uses the combination of the EpiTect Fast Bisulfite Kit – for bisulfite conversion of non-methylated cytosines – along with the end-polishing and ligation chemistries of the QIAseq Ultralow Input Library Kit.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, March 27, 2017 at 1:00 PM Eastern    Status: Available Reserve

Using Workflows to perform integrated analysis of mutational pattern and expression in RNA-seq data

"In this webinar, we demonstrate how to perform integrated analysis of resequencing and expression data arising from a RNA-seq experiment using Workflows in the Genomics Workbench. The session will include a brief presentation as well as a short live demo with a small NGS dataset. The following topics will be covered during this session: • Creating and running end-to-end Workflows in Genomics • Overview of resequencing tools • Overview of RNA-seq expression analysis • Viewing results using Track List "

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, March 30, 2017 at 4:00 AM Eastern    Status: Available Reserve
Thursday, March 30, 2017 at 1:00 PM Eastern    Status: Available Reserve

Note: Viewers will be asked to register before viewing the previously recorded webinars.