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Online Seminars

SABiosciences is pleased to provide free, on-line educational resources covering relevant tools and techniques for gene and protein expression analysis, and cell-based gene function analysis. We offer live webinars, prerecorded and PDF formatted presentations.

See the instructions for the Web Seminars

September 2017
Monday
Tuesday
Wednesday
Thursday
Friday

1

4

5

Nucleic acid purification on QIAcube

6

Targeted RNA-seq for gene expression

Targeted RNA-seq for gene expression

7

8

Introducing Unique Molecular Indices technology

11

Targeted DNA-seq for mutation detection

12

Genotyping and microbial identification on Rotor-Gene Q

miRNA-seq in liquid biopsy

13

RNAseq data analysis with Biomedical GW

RNAseq data analysis with Biomedical GW

14

gDNA collection, storage, and purification

NGS in single-cell applications

15

Practical hints for successful PCR

Practical hints for successful PCR

18

Circulating biomarker isolation from liquid biopsy samples

19

Sample QC and reproducibility

20

Biologically interpret RNAseq data with IPA

Biologically interpret RNAseq data with IPA

21

gDNA isolation from solid tissue samples

22

25

Circulating miRNA analysis with NGS

Single-cell RNA-seq - QIAscout & QIAseq FX Single Cell RNA Library Kit

26

DNA methylation analysis on PyroMark Q48 Autoprep

27

28

Critical factors for successful multiplex qPCR

Critical factors for successful multiplex qPCR

29

Current Seminar Titles Available:

Focus Title
1.  Multiplex PCRPractical hints and new solutions for successful real-time PCR studies
2.  Multiplex PCRCritical Factors for Successful Multiplex Real-Time PCR
3.  DNA sample isolationTips and Tricks for Rapid Isolation of Genomic DNA from Solid Tissue Samples
4.  DNA sample isolationFundamental Concepts and Special Considerations in gDNA Isolation for Better Insight
5.  QIAseq miRNA Library KitmiRNA-seq from liquid biopsy: robust detection from the lowest sample amounts
6.  QIAseq NGSDigital sequencing: Introduction to Unique Molecular Index (UMI) technology for targeted sequencing
7.  QIAseq NGSTargeted DNA-seq for mutation detection
8.  QIAseq NGSGene expression analysis using targeted RNA-seq and desktop NGS instruments
9.  QIAseq NGSNext-generation sequencing in single-cell applications
10.  NGS: Now integrated and connected with your lab operationsNGS: Now integrated and connected with your lab operations
11.  QIAseq and QIAscoutSingle-cell RNA-seq using live cells and microrafts – linking cell morphology to transcriptomics
12.  Liquid BiopsiesIdentification and interpretation of pathogenic variants in cell-free DNA – what are the challenges and how to overcome them
13.  AutomationAutomated nucleic acid purification from diverse sample types using dedicated microbiome kits on the QIAcube
14.  AutomationRotor-Gene Q: A Rapid, Automated Real-Time PCR Instrument for Genotyping and Microbial Identification with Excellent Efficiency, Reproducibility and Support
15.  AutomationKeeping control over the quality and reproducibility of your research – know how
16.  AutomationDNA methylation analysis in a single day – the new PyroMark Q48 Autoprep
17.  BioinformaticsAnalyzing QIAseq Targeted RNA Panels data using the Biomedical Genomics Workbench
18.  BioinformaticsBiologically interpret your RNA-seq data with knowledge-based IPA
19.  ExosomesCirculating biomarkers – what matters for the isolation of biomarkers from liquid biopsy samples?
20.  QIAseq NGSCirculating miRNA and RNA identification for biomarker assessment

Practical hints and new solutions for successful real-time PCR studies

In this webinar we will cover the following topics which are critical steps for efficient and precise gene expression studies using real-time PCR technology: - Effect of RNA integrity on real-time PCR results – tips to achieve a true RNA profiling suitable for real-time PCR studies - Improved methods for cDNA synthesis, optimized for real-time PCR - Real-time PCR analysis • Real-time PCR essentials and background information on different quantification strategies • SYBR Green real-time PCR – factors influencing specificity • Introduction to probe technology • New, fast and efficient real-time PCR solutions

Duration: 45 minutes followed by Q&A session.

Schedule:

Friday, September 15, 2017 at 9:30 AM Eastern    Status: Available Reserve
Friday, September 15, 2017 at 1:00 PM Eastern    Status: Available Reserve

Critical Factors for Successful Multiplex Real-Time PCR

Multiplex real-time PCR is a powerful tool for gene expression analysis, viral load monitoring, genotyping, and many other applications. The ability to amplify and detect several genomic DNA, cDNA, or RNA targets in the same reaction offers many benefits: • Conservation of precious samples – more quantification data per sample • Increased throughput – more targets analyzed per run on a cycler • Reliable results – no well-to-well variability due to co-amplification of internal control • Reduced costs – save time and reagents The QuantiFast Multiplex PCR and RT-PCR kits are optimized for reliable amplification of many different templates despite a high variability in abundance. Thus they enable successful amplification of multiple targets on the first attempt without optimization. This webinar explains the principles of the QIAGEN multiplex technologies and shows data demonstrating the exceptional multiplex real-time PCR performance of the QuantiFast Multiplex kits.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, September 28, 2017 at 9:30 AM Eastern    Status: Available Reserve
Thursday, September 28, 2017 at 1:00 PM Eastern    Status: Available Reserve

Tips and Tricks for Rapid Isolation of Genomic DNA from Solid Tissue Samples

Obtaining reliable results from genotyping or sequencing experiments using solid tissue is not always easy. In order to have a successful experiment, you must start with sufficient amounts of high-quality DNA. An inefficient lysis results in poor yields, low DNA integrity and consequently, inferior quality results, thereby limiting the insights you can attain from your sample. In this webinar, we will talk about solutions to overcome the challenges of lysis of fibrous or hard tissue so that you can achieve the insights you value.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, September 21, 2017 at 1:00 PM Eastern    Status: Available Reserve

Fundamental Concepts and Special Considerations in gDNA Isolation for Better Insight

"How are your gDNA yields? Some sample types present special challenges in DNA purification and analysis. This webinar provides tips for a whole range of sample types that require special consideration. We will cover the following topics in detail: • The basic methods and challenges of gDNA purification • Working with DNA: good laboratory practice • Special considerations for challenging sample types • Performing DNA quality checks"

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, September 14, 2017 at 1:00 PM Eastern    Status: Available Reserve

miRNA-seq from liquid biopsy: robust detection from the lowest sample amounts

miRNAs impact virtually all areas of biology, and in circulation, they are promising biomarker candidates for both normal and disease biology. miRNAs are protected from degradation in virtually all biofluids by exosomes, Ago2, HDL or other protective proteins, but are expressed at low levels. As a result, expression analysis, particularly using next-generation sequencing (NGS), has proven to be difficult. Traditional small RNA library kits lack the sensitivity or specificity to adequately assess miRNA expression. Libraries prepared with using these kits have been fraught with background products, such as adapter dimers and other RNAs, including hY4 Y RNA. These problems collectively manifest as a low mapping percentage to miRNA, a limited dynamic range and lost discovery potential. QIAGEN’s QIAseq miRNA Library Kit is specifically designed to overcome these challenges. The innovative, gel-free workflow enables the preparation of robust libraries from even the most difficult, low RNA content biofluids. QIAseq miRNA maximizes your on-target miRNA reads, dynamic range and, mostly importantly, your discovery potential.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, September 12, 2017 at 4:00 PM Eastern    Status: Available Reserve

Digital sequencing: Introduction to Unique Molecular Index (UMI) technology for targeted sequencing

Errors related to the use of PCR in library construction have plagued targeted DNA and RNA sequencing experiments, calling into question the accuracy of results. Digital sequencing overcomes these challenges by introducing Unique Molecular Indices, or UMIs. These molecular barcodes are incorporated into the DNA or RNA sequences before any amplification takes place, eliminating errors such as PCR duplication and amplification bias. This webinar features an in-depth discussion of how UMI technology works and an introduction to how it is used in QIAGEN’s QIAseq sequencing solutions. Find out how to improve your NGS data with the accuracy of UMIs!

Duration: 45 minutes followed by Q&A session.

Schedule:

Friday, September 8, 2017 at 4:00 PM Eastern    Status: Available Reserve

Targeted DNA-seq for mutation detection

The utilization of targeted DNA sequencing with enrichment panels is on the rise for detecting genetic variants in cancers, inherited diseases and more. In this webinar, we introduce the QIAseq Targeted DNA Panels, which use Unique Molecular Indices (UMIs) and Single Primer Extension (SPE) technology to improve downstream analysis of difficult-to-sequence genes on Illumina and Thermo Fisher NGS instruments. QIAGEN uses its proprietary knowledge base to design panels for different cancer types, carrier testing, cardiomyopathy and other areas of interest. In addition, QIAGEN offers full coverage of the human genome, allowing researchers to build custom panels for their specific applications. Join us to find out how you can quickly and reliably detect genetic variants in your research from FFPE and fresh samples.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, September 11, 2017 at 4:00 PM Eastern    Status: Available Reserve

Gene expression analysis using targeted RNA-seq and desktop NGS instruments

Not every experiment requires whole transcriptome sequencing, but until recently, there hasn’t been a convenient way to profile transcriptomes in biological research areas such as cancer or immunity. In this webinar, we discuss the strategy and technology of targeted RNA sequencing, including the use of novel targeted RNA-seq panels covering the transcriptomes of 8 different pathways for human and mouse, as well as 170+ smaller panels. Using Single Primer Extension (SPE) and Unique Molecular Index (UMI) technology, the QIAseq panels overcome traditional RNA-seq limitations and ensure increased precision and accuracy. Join us and learn how you can easily upgrade your RNA-seq data and move beyond the limits of qPCR, microarray and traditional RNA-seq.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, September 6, 2017 at 9:30 AM Eastern    Status: Available Reserve
Wednesday, September 6, 2017 at 1:00 PM Eastern    Status: Available Reserve

Next-generation sequencing in single-cell applications

Single-cell sequencing is revolutionizing how we understand biology by revealing the individual contributions of cells rather than simply analyzing in bulk. QIAGEN has developed complete cell-to-library solutions for DNA and RNA sequencing libraries from single cells, as well as the novel QIAscout system for reliable single-cell isolation. In this webinar, we discuss several applications for single-cell NGS, along with application data. Find out how to apply NGS to your single-cell research!

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, September 14, 2017 at 4:00 PM Eastern    Status: Available Reserve

NGS: Now integrated and connected with your lab operations

Are you choosing an NGS solution that offers connectivity with the rest of your laboratory operations? Imagine being able to track samples, record reagent lots, store results and manage workflows, all with the help of one easy software interface. In this webinar you will learn how to do this with the QIAGEN GeneRead Link Software.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, August 23, 2017 at 4:00 AM Eastern    Status: Available Reserve

Single-cell RNA-seq using live cells and microrafts – linking cell morphology to transcriptomics

"Dose-response studies and the analysis of involved genes and pathways is a key application in multiple research areas, especially in drug toxicity and cancer research. Single-cell analysis enables scientists to understand responses of individual cells by providing valuable information about gene expression variability. In this webinar, we will introduce a robust and easy-to-use single-cell RNA-seq workflow for studying cells selected for analysis based on morphological alterations after drug treatment. We will demonstrate an innovative microraft-based technology, the QIAscout, which is used with a standard microscope to isolate live single cells after being treated with PMA (phorbol 12-myristate 13-acetate). Transcriptome data from RNA-seq of responder versus non-responder cells analyzed using the QIAseq FX Single Cell RNA Library Kit will be presented. We will also provide further details on QIAGEN’s single-cell analysis workflow, which offers a simple and ideal platform to perform dose-response and time-course studies based on live cell morphology, allowing scientists to link cell biology to transcriptomics. If you’re working with live eukaryotic cells, join us and learn how you can quickly adapt this workflow to your lab."

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, September 25, 2017 at 1:00 PM Eastern    Status: Available Reserve

Identification and interpretation of pathogenic variants in cell-free DNA – what are the challenges and how to overcome them

"Several studies have shown that the analysis of cfDNA enables the detection of pathogenic variants, which can be important for treatment decisions. The use of cfDNA has many advantages as it does not require surgery. Moreover, it may help in the detection of early tumor recurrence and upcoming resistance. However, due to dilution from non-cancerous cells, variants are expected at a very low allele percentage, which makes it very hard to detect them and to separate them from false positives. In addition, the interpretation of data in terms of being able to identify pathogenic variants can be challenging as well. In this webinar, we will show how cfDNA data from next-generation sequencing can be analyzed with a high level of accuracy. In a case study, we will compare variants identified in cfDNA with those identified in the buffy coat and tumor, and show potential new candidates. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, August 29, 2017 at 9:30 AM Eastern    Status: Available Reserve

Automated nucleic acid purification from diverse sample types using dedicated microbiome kits on the QIAcube

"The field of microbiome research continues to expand and operate at a much larger scale, creating unique challenges for analysis. Inherent complexities in the composition of microbiome samples, such as stool, soil, water and biofilm, can lead to inefficient lysis and result in an inaccurate representation of the microbial content. Additionally, these samples contain small molecule inhibitors that may cause unreliable quantification of nucleic acids and negatively impact downstream applications such as quantitative PCR (qPCR) and next-generation sequencing (NGS). Optimized bead beating and patented Inhibitor Removal Technology (IRT) are two innovative features of the new microbiome kits that have enabled lysis of even the toughest samples and successful removal of inhibitors during the purification process. This webinar will focus on the automation of QIAGEN’s new line of DNA and RNA sample prep kits for the microbiome. The microbiome of samples as diverse as soil, stool, water and biofilm can be purified using dedicated QIAcube compatible kits. Traditional, labor-intensive and time-consuming manual purification steps can now be replaced by automating the IRT and purification steps of these new microbiome kits, saving valuable time and ensuring standardized results. We will show how automation on the QIAcube enables efficient and reliable use of these samples for sensitive downstream applications such as qPCR and NGS. In addition, you will learn how to successfully use the CLC Microbial Genomics Module for metagenome sequencing and identification of microbial composition and diversity."

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, September 5, 2017 at 1:00 PM Eastern    Status: Available Reserve

Rotor-Gene Q: A Rapid, Automated Real-Time PCR Instrument for Genotyping and Microbial Identification with Excellent Efficiency, Reproducibility and Support

"Molecular testing for the identification of bacterial and viral infections as well as gene mutations from raw livestock samples and medical research specimens is always a challenging task. It requires a multistep approach to optimize assays and to eliminate inhibitory effects in PCR amplification. Laboratory benchtop PCR assay setup procedures can be very labor intensive and unreliable, which is reflected in its prolonged assay time, poor reproducibility and increasing total assay costs. The need for automation has led to the development and introduction of robotic laboratory instruments, aiming to decrease operator errors and process time, and to increase process safety. We have developed a selection of robust, novel chemistries to prevent PCR crosstalk. We can successfully measure target abundance and fold change in real-time assays, and perform sub-genotyping using a fast, high-throughput and powerful High-Resolution Melting (HRM) statistical analysis program. We have also tested a liquid handler for quick PCR assay setup to eliminate manual steps and increase productivity. In this presentation, we will demonstrate these features and benefits with examples."

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, September 12, 2017 at 1:00 PM Eastern    Status: Available Reserve

Keeping control over the quality and reproducibility of your research – know how

From starting material to final results, every analysis workflow is a journey to unlock the biological information within the samples without altering it. Within each step of the workflow lies challenges directly related to the sample type and analysis technologies, and at each step, lies a potential chance of error, jeopardizing your experiments and results. Therefore, performing relevant quality control after critical steps and standardizing sample parameters are of utmost importance to ensure reproducibility of the results and their reliable interpretation. In this webinar, we will introduce you to the key sample quality parameters, discuss their respective impact on downstream applications and how to monitor them, and present the advantages of automating quality control along complex workflows.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, September 19, 2017 at 1:00 PM Eastern    Status: Available Reserve

DNA methylation analysis in a single day – the new PyroMark Q48 Autoprep

"Based on the sequencing-by-synthesis principle, Pyrosequencing is a highly flexible technology for rapid and quantitative analysis of any type of sequence variation. The real-time output delivers high-resolution sequence information, making Pyrosequencing highly suitable for various applications, particularly for DNA methylation quantification. Combined with the latest EpiTect Fast bisulfite conversion technology, the new PyroMark Q48 Autoprep can now provide highly automated methylation analysis in a single day. This webinar will focus on the following topics: • How bisulfite conversion can be improved for more reliable methylation results • How 5-hmC can be differentiated from 5-mC • Why Pyrosequencing is ideally suited for sensitive methylation analysis • What does “advanced” Pyrosequencing offer for methylation analysis • How the new PyroMark Q48 Autoprep streamlines the workflow for methylation analysis"

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, September 26, 2017 at 1:00 PM Eastern    Status: Available Reserve

Analyzing QIAseq Targeted RNA Panels data using the Biomedical Genomics Workbench

"This webinar will provide details on how to analyze data obtained from QIAseq Targeted RNA Panels with tools in the Biomedical Genomics Workbench. The session will include a live demo involving analysis of a small dataset. We will be covering the following topics during the webinar: • Recap of QIAseq Targeted RNA Panels and UMI technology • Download of reference data and import of raw sequencing reads and target regions • Installing plugins • Trimming and quantification of data using a ready-to-use workflow • Differential expression analysis • Visualization of data • Upload of results to Ingenuity Pathway Analysis (IPA)"

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, September 13, 2017 at 9:30 AM Eastern    Status: Available Reserve
Wednesday, September 13, 2017 at 1:00 PM Eastern    Status: Available Reserve

Biologically interpret your RNA-seq data with knowledge-based IPA

"In this webinar, we will show the Expression Analysis results generated from a QIAseq Targeted RNA-seq panel dataset. You can find out how to view and interpret your Expression Analysis results in IPA and learn about the multiple ways of relating the molecules in your dataset to the body of information in the Ingenuity Knowledge Base. Discover more about: • Signaling and metabolic canonical pathways enriched in your data • Biological functions and diseases that are over-represented in your data • Predicted upstream regulators that may explain the expression changes observed in your data • How IPA’s integration of analysis results creates causal hypotheses about how upstream regulators influence downstream phenotypes and biological functions • Interaction networks (algorithmically-generated pathways describing potential molecular interactions in your experimental system) • Molecules and biological processes that are predicted to be activated or inhibited in your experimental system"

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, September 20, 2017 at 9:30 AM Eastern    Status: Available Reserve
Wednesday, September 20, 2017 at 1:00 PM Eastern    Status: Available Reserve

Circulating biomarkers – what matters for the isolation of biomarkers from liquid biopsy samples?

"The analysis of circulating biomarkers in a patient’s blood holds significant potential for early disease detection and monitoring. Circulating DNA is the most commonly studied biomarker in the context of liquid biopsy research. However, in the last couple of years, free-circulating RNA has gained more attention for biomarker analysis and interpretation. In this webinar, we will discuss the different types of circulating RNA biomarkers studied in liquid biopsy samples. A special focus will be given to the challenges and solutions for isolating circulating RNA."

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, September 18, 2017 at 9:30 AM Eastern    Status: Available Reserve

Circulating miRNA and RNA identification for biomarker assessment

"Circulating RNA can serve as a powerful biomarker for patient stratification and assessment of overall health and prognosis. In this webinar, we will focus on using next-generation sequencing (NGS) for identification of miRNA and mRNAs from biofluids, including best practices for designing and executing a successful experiment. While RNA is vulnerable to degradation in serum/plasma due to the abundance of RNAseq and other degrading enzymes, mRNA and miRNA are protected within exosomes and other type of vesicles. Enrichment of exosomes from biofluids provides a supply of RNA biomarkers which can be profiled by NGS and further verified using targeted sequencing or qPCR. In this webinar, we will show how QIAGEN’s QIAseq miRNA Library Kit with Unique Molecular Indexes (UMIs) gives researchers several advantages compared to other small RNA sequencing kits. QIAseq miRNA delivers a gel-free workflow down to 1 ng of total RNA input and its proprietary chemistry minimizes ligation and increases the number of miRNA reads by actively blocking non-relevant, contaminating side products and RNAs. Other kits on the market may include a gel-free workflow or reduced ligation bias, but their protocols and methods fall short on delivering these promises. With the inclusion of UMIs in the QIAseq miRNA Library Kit, researchers are ensured that they have sequenced deep enough to cover the entire library and PCR and sequencing bias and errors are removed. In addition, we will address workflows for exosome mRNA discovery using stranded RNAseq and subsequent verification using targeted RNA-seq. QIAGEN’s exosome RNA discovery pipeline starts with a global view of the expressed RNA which is accurately identified using QIAGEN Biomedical Workbench’s tunable algorithm and verified using QIAseq Targeted RNA Panels which employ UMIs to ensure accurate quantification and adequate sequencing depth for each sample you run. Come listen to the RNA experts discuss the ‘next generation’ of sequencing-based workflows for biomarker detection and verification!"

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, September 25, 2017 at 9:30 AM Eastern    Status: Available Reserve

Note: Viewers will be asked to register before viewing the previously recorded webinars.